AU740238B2 - Method for polynucleotide sequencing - Google Patents
Method for polynucleotide sequencing Download PDFInfo
- Publication number
- AU740238B2 AU740238B2 AU38784/97A AU3878497A AU740238B2 AU 740238 B2 AU740238 B2 AU 740238B2 AU 38784/97 A AU38784/97 A AU 38784/97A AU 3878497 A AU3878497 A AU 3878497A AU 740238 B2 AU740238 B2 AU 740238B2
- Authority
- AU
- Australia
- Prior art keywords
- primer
- template
- polynucleotide
- analog
- polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims description 90
- 108091033319 polynucleotide Proteins 0.000 title claims description 66
- 102000040430 polynucleotide Human genes 0.000 title claims description 66
- 239000002157 polynucleotide Substances 0.000 title claims description 66
- 238000012163 sequencing technique Methods 0.000 title claims description 33
- 125000003729 nucleotide group Chemical group 0.000 claims description 66
- 239000002773 nucleotide Substances 0.000 claims description 64
- -1 nucleoside triphosphate Chemical class 0.000 claims description 56
- 239000002777 nucleoside Substances 0.000 claims description 53
- 239000001226 triphosphate Substances 0.000 claims description 47
- 235000011178 triphosphate Nutrition 0.000 claims description 47
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- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 18
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- 101001093748 Homo sapiens Phosphatidylinositol N-acetylglucosaminyltransferase subunit P Proteins 0.000 description 4
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- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- XPDXVDYUQZHFPV-UHFFFAOYSA-N Dansyl Chloride Chemical compound C1=CC=C2C(N(C)C)=CC=CC2=C1S(Cl)(=O)=O XPDXVDYUQZHFPV-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
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- 230000005257 nucleotidylation Effects 0.000 description 2
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- NPEIGRBGMUJNFE-UHFFFAOYSA-N 1-aminohexan-1-ol Chemical compound CCCCCC(N)O NPEIGRBGMUJNFE-UHFFFAOYSA-N 0.000 description 1
- AKIZPWSPNKVOMT-UHFFFAOYSA-N 1-sulfanylhexan-1-ol Chemical compound CCCCCC(O)S AKIZPWSPNKVOMT-UHFFFAOYSA-N 0.000 description 1
- AEUVIXACNOXTBX-UHFFFAOYSA-N 1-sulfanylpropan-1-ol Chemical compound CCC(O)S AEUVIXACNOXTBX-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66521096A | 1996-06-14 | 1996-06-14 | |
| US08/665210 | 1996-06-14 | ||
| PCT/US1997/009664 WO1997047761A1 (en) | 1996-06-14 | 1997-06-13 | Method for polynucleotide sequencing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU3878497A AU3878497A (en) | 1998-01-07 |
| AU740238B2 true AU740238B2 (en) | 2001-11-01 |
Family
ID=24669185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU38784/97A Ceased AU740238B2 (en) | 1996-06-14 | 1997-06-13 | Method for polynucleotide sequencing |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5908755A (OSRAM) |
| EP (1) | EP0912752A1 (OSRAM) |
| JP (1) | JP2000512498A (OSRAM) |
| AU (1) | AU740238B2 (OSRAM) |
| CA (1) | CA2258511A1 (OSRAM) |
| IL (1) | IL127560A0 (OSRAM) |
| WO (1) | WO1997047761A1 (OSRAM) |
Families Citing this family (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5830655A (en) | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
| US6391622B1 (en) | 1997-04-04 | 2002-05-21 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
| US6235471B1 (en) * | 1997-04-04 | 2001-05-22 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
| US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| GB9828785D0 (en) * | 1998-12-30 | 1999-02-17 | Amersham Pharm Biotech Ab | Sequencing systems |
| WO2000058507A1 (en) * | 1999-03-30 | 2000-10-05 | Solexa Ltd. | Polynucleotide sequencing |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US7332275B2 (en) | 1999-10-13 | 2008-02-19 | Sequenom, Inc. | Methods for detecting methylated nucleotides |
| EP1228244A4 (en) * | 1999-11-04 | 2005-02-09 | California Inst Of Techn | METHODS AND DEVICES FOR ANALYZING POLYNUCLEOTIDE SEQUENCES |
| US6958214B2 (en) | 2000-07-10 | 2005-10-25 | Sequenom, Inc. | Polymorphic kinase anchor proteins and nucleic acids encoding the same |
| GB0021286D0 (en) * | 2000-08-30 | 2000-10-18 | Gemini Genomics Ab | Identification of drug metabolic capacity |
| EP1790736A3 (en) | 2000-10-06 | 2007-08-15 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| US6720148B1 (en) * | 2001-02-22 | 2004-04-13 | Caliper Life Sciences, Inc. | Methods and systems for identifying nucleotides by primer extension |
| US7110885B2 (en) | 2001-03-08 | 2006-09-19 | Dnaprint Genomics, Inc. | Efficient methods and apparatus for high-throughput processing of gene sequence data |
| EP1368497A4 (en) * | 2001-03-12 | 2007-08-15 | California Inst Of Techn | METHODS AND APPARATUS FOR ANALYZING ASYNCHRONOUS BASE EXTENSION POLYNUCLEOTIDE SEQUENCES |
| JP3871301B2 (ja) * | 2001-05-15 | 2007-01-24 | インターナショナル・ビジネス・マシーンズ・コーポレーション | データベース検索装置、及びプログラム |
| WO2003054143A2 (en) * | 2001-10-25 | 2003-07-03 | Neurogenetics, Inc. | Genes and polymorphisms on chromosome 10 associated with alzheimer's disease and other neurodegenerative diseases |
| US20030170678A1 (en) * | 2001-10-25 | 2003-09-11 | Neurogenetics, Inc. | Genetic markers for Alzheimer's disease and methods using the same |
| US20030224380A1 (en) * | 2001-10-25 | 2003-12-04 | The General Hospital Corporation | Genes and polymorphisms on chromosome 10 associated with Alzheimer's disease and other neurodegenerative diseases |
| CA2464682A1 (en) * | 2001-10-26 | 2003-05-01 | Sequenom, Inc. | Method and apparatus for high-throughput sample handling process line |
| US7432342B2 (en) | 2002-05-03 | 2008-10-07 | Sequenom, Inc. | Kinase anchor protein muteins, peptides thereof and related documents |
| US20030220844A1 (en) * | 2002-05-24 | 2003-11-27 | Marnellos Georgios E. | Method and system for purchasing genetic data |
| EP2112229A3 (en) | 2002-11-25 | 2009-12-02 | Sequenom, Inc. | Methods for identifying risk of breast cancer and treatments thereof |
| JP4395133B2 (ja) | 2002-12-20 | 2010-01-06 | カリパー・ライフ・サイエンシズ・インク． | Ｄｎａの単一分子増幅および検出 |
| WO2004072294A2 (en) * | 2003-02-12 | 2004-08-26 | Genizon Svenska Ab | Methods and means for nucleic acid sequencing |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| EP1716254B1 (en) | 2004-02-19 | 2010-04-07 | Helicos Biosciences Corporation | Methods for analyzing polynucleotide sequences |
| DE602005027700D1 (de) | 2004-05-25 | 2011-06-09 | Helicos Biosciences Corp | Verfahren zur nukleinsäureimmobilisierung |
| US7476734B2 (en) | 2005-12-06 | 2009-01-13 | Helicos Biosciences Corporation | Nucleotide analogs |
| EP1773860A4 (en) * | 2004-07-22 | 2009-05-06 | Sequenom Inc | METHOD FOR IDENTIFYING THE RISK OF TYPE II DIABETES AND TREATING THEREOF |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| US7397546B2 (en) | 2006-03-08 | 2008-07-08 | Helicos Biosciences Corporation | Systems and methods for reducing detected intensity non-uniformity in a laser beam |
| US20080309926A1 (en) * | 2006-03-08 | 2008-12-18 | Aaron Weber | Systems and methods for reducing detected intensity non uniformity in a laser beam |
| EP3617321B1 (en) * | 2006-05-31 | 2024-10-23 | Sequenom, Inc. | Kit for the extraction and amplification of nucleic acid from a sample |
| US8153369B2 (en) | 2006-06-05 | 2012-04-10 | Cancer Care Ontario | Assessment of risk for colorectal cancer |
| GB2457402B (en) | 2006-12-01 | 2011-10-19 | Univ Columbia | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| US7902345B2 (en) | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
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- 1997-06-13 AU AU38784/97A patent/AU740238B2/en not_active Ceased
- 1997-06-13 CA CA002258511A patent/CA2258511A1/en not_active Abandoned
- 1997-06-13 IL IL12756097A patent/IL127560A0/xx unknown
- 1997-06-13 WO PCT/US1997/009664 patent/WO1997047761A1/en not_active Ceased
- 1997-06-13 JP JP10501677A patent/JP2000512498A/ja active Pending
- 1997-06-13 EP EP97936015A patent/EP0912752A1/en not_active Withdrawn
- 1997-10-15 US US08/950,709 patent/US5908755A/en not_active Expired - Lifetime
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| WO1990013666A1 (en) * | 1989-05-11 | 1990-11-15 | Amersham International Plc | Sequencing method |
| US5403708A (en) * | 1992-07-06 | 1995-04-04 | Brennan; Thomas M. | Methods and compositions for determining the sequence of nucleic acids |
Also Published As
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|---|---|
| AU3878497A (en) | 1998-01-07 |
| EP0912752A1 (en) | 1999-05-06 |
| JP2000512498A (ja) | 2000-09-26 |
| WO1997047761A1 (en) | 1997-12-18 |
| IL127560A0 (en) | 1999-10-28 |
| CA2258511A1 (en) | 1997-12-18 |
| US5908755A (en) | 1999-06-01 |
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