WO1990013666A1 - Sequencing method - Google Patents
Sequencing method Download PDFInfo
- Publication number
- WO1990013666A1 WO1990013666A1 PCT/GB1990/000726 GB9000726W WO9013666A1 WO 1990013666 A1 WO1990013666 A1 WO 1990013666A1 GB 9000726 W GB9000726 W GB 9000726W WO 9013666 A1 WO9013666 A1 WO 9013666A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- template
- polymerisation
- nucleotide
- event
- primer
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8910880.7 | 1989-05-11 | ||
GB898910880A GB8910880D0 (en) | 1989-05-11 | 1989-05-11 | Sequencing method |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1990013666A1 true WO1990013666A1 (en) | 1990-11-15 |
Family
ID=10656602
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB1990/000726 WO1990013666A1 (en) | 1989-05-11 | 1990-05-11 | Sequencing method |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0471732A1 (en) |
JP (1) | JPH04505251A (en) |
CA (1) | CA2045505A1 (en) |
GB (1) | GB8910880D0 (en) |
WO (1) | WO1990013666A1 (en) |
Cited By (94)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991006678A1 (en) * | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
WO1993006241A1 (en) * | 1991-09-16 | 1993-04-01 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Gene probe biosensor method |
WO1993021340A1 (en) * | 1992-04-22 | 1993-10-28 | Medical Research Council | Dna sequencing method |
US5547839A (en) * | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
WO1997004129A1 (en) * | 1995-07-14 | 1997-02-06 | Biacore Ab | Method for nucleic acid sequencing |
US5641634A (en) * | 1995-11-30 | 1997-06-24 | Mandecki; Wlodek | Electronically-indexed solid-phase assay for biomolecules |
WO1997027331A2 (en) * | 1996-01-23 | 1997-07-31 | Rapigene, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
WO1997047761A1 (en) * | 1996-06-14 | 1997-12-18 | Sarnoff Corporation | Method for polynucleotide sequencing |
US5736332A (en) * | 1995-11-30 | 1998-04-07 | Mandecki; Wlodek | Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders |
WO1998039485A2 (en) * | 1997-03-05 | 1998-09-11 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
WO1999005320A1 (en) * | 1997-07-22 | 1999-02-04 | Rapigene, Inc. | Multiple functionalities within an array element and uses thereof |
WO1999005321A1 (en) * | 1997-07-22 | 1999-02-04 | Rapigene, Inc. | Amplification and other enzymatic reactions performed on nucleic acid arrays |
WO1999005315A2 (en) * | 1997-07-28 | 1999-02-04 | Medical Biosystems Ltd. | Nucleic acid sequence analysis |
WO1999054497A1 (en) * | 1998-04-22 | 1999-10-28 | Stefan Seeger | Method and device for determining the concentration, adsorption and binding kinetics and equilibrium and binding constants of molecules by luminescence measurements |
US5981166A (en) * | 1997-04-23 | 1999-11-09 | Pharmaseq, Inc. | Screening of soluble chemical compounds for their pharmacological properties utilizing transponders |
WO1999057321A1 (en) * | 1998-05-01 | 1999-11-11 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and dna molecules |
US6027890A (en) * | 1996-01-23 | 2000-02-22 | Rapigene, Inc. | Methods and compositions for enhancing sensitivity in the analysis of biological-based assays |
WO2000018956A1 (en) * | 1998-09-30 | 2000-04-06 | Molecular Machines & Industries Gmbh | Method for dna- or rna-sequencing |
US6051377A (en) * | 1995-11-30 | 2000-04-18 | Pharmaseq, Inc. | Multiplex assay for nucleic acids employing transponders |
WO2000053805A1 (en) * | 1999-03-10 | 2000-09-14 | Asm Scientific, Inc. | A method for direct nucleic acid sequencing |
WO2000058507A1 (en) * | 1999-03-30 | 2000-10-05 | Solexa Ltd. | Polynucleotide sequencing |
WO2000060114A2 (en) * | 1999-04-06 | 2000-10-12 | Medical Biosystems Ltd. | Polynucleotide sequencing using a helicase |
US6136543A (en) * | 1997-01-31 | 2000-10-24 | Hitachi, Ltd. | Method for determining nucleic acids base sequence and apparatus therefor |
US6153379A (en) * | 1993-06-22 | 2000-11-28 | Baylor College Of Medicine | Parallel primer extension approach to nucleic acid sequence analysis |
US6197557B1 (en) | 1997-03-05 | 2001-03-06 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
WO2001025480A2 (en) * | 1999-10-06 | 2001-04-12 | Medical Biosystems Ltd. | Dna sequencing method |
WO2001032930A1 (en) * | 1999-11-04 | 2001-05-10 | California Institute Of Technology | Methods and apparatuses for analyzing polynucleotide sequences |
US6242193B1 (en) | 1999-07-30 | 2001-06-05 | Hitachi, Ltd. | Apparatus for determining base sequence of nucleic acid |
WO2001057249A1 (en) * | 2000-02-02 | 2001-08-09 | Solexa Ltd. | Synthesis of spatially addressed molecular arrays |
WO2001057248A2 (en) * | 2000-02-01 | 2001-08-09 | Solexa Ltd. | Polynucleotide arrays and their use in sequencing |
WO2001073121A1 (en) * | 2000-03-30 | 2001-10-04 | Toyota Jidosha Kabushiki Kaisha | Method of determining base sequence of single nucleic acid molecule |
US6312893B1 (en) | 1996-01-23 | 2001-11-06 | Qiagen Genomics, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
EP1163369A1 (en) * | 1999-02-23 | 2001-12-19 | Caliper Technologies Corporation | Sequencing by incorporation |
WO2002002813A2 (en) * | 2000-07-05 | 2002-01-10 | Amersham Biosiences Uk Ltd. | Sequencing method |
EP1179085A1 (en) * | 1999-05-19 | 2002-02-13 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US6355433B1 (en) | 2000-06-02 | 2002-03-12 | Dna Sciences, Inc. | Determination of nucleotide sequence variations through limited primer extension |
US6361950B1 (en) | 1995-11-30 | 2002-03-26 | Pharmaseq, Inc. | Multiplex assay for nucleic acids employing transponders |
US6365349B1 (en) | 1997-07-22 | 2002-04-02 | Qiagen Genomics, Inc. | Apparatus and methods for arraying solution onto a solid support |
US6387623B1 (en) | 1995-11-30 | 2002-05-14 | Pharmaseq | Screening of drugs from chemical combinatorial libraries employing transponders |
US6458544B1 (en) | 1999-12-02 | 2002-10-01 | Dna Sciences, Inc. | Methods for determining single nucleotide variations and genotyping |
US6511803B1 (en) * | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
US6573047B1 (en) | 1999-04-13 | 2003-06-03 | Dna Sciences, Inc. | Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation |
US6613508B1 (en) | 1996-01-23 | 2003-09-02 | Qiagen Genomics, Inc. | Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques |
WO2003073088A2 (en) * | 2001-03-09 | 2003-09-04 | Christofer Toumazou | Apparatus and method for the detection of localised fluctuactions of ionic charge by ion sensitive field effect transistors during a chemical reaction |
US6653082B2 (en) | 2001-05-17 | 2003-11-25 | Baylor College Of Medicine | Substrate-bound cleavage assay for nucleic acid analysis |
WO2004029294A1 (en) * | 2002-09-27 | 2004-04-08 | Biotage Ab | New sequencing method for sequencing rna molecules |
WO2004072294A2 (en) * | 2003-02-12 | 2004-08-26 | Genizon Svenska Ab | Methods and means for nucleic acid sequencing |
US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
WO2004106545A1 (en) * | 2003-05-28 | 2004-12-09 | Innogenetics N.V. | Methods for enhanced detection using surface sensitive techniques. |
US6908736B1 (en) | 1999-10-06 | 2005-06-21 | Medical Biosystems, Ltd. | DNA sequencing method |
WO2005118871A1 (en) * | 2004-05-28 | 2005-12-15 | The Arizona Board Of Regents | Surface plasmon resonance sensor for detecting changes in polynucleotides mass |
US7001722B1 (en) | 1993-06-22 | 2006-02-21 | Baylor College Of Medicine | Parallel primer extension approach to nucleic acid sequence analysis |
EP1681356A1 (en) * | 1999-05-19 | 2006-07-19 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US7170050B2 (en) | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
US7270958B2 (en) | 1998-09-10 | 2007-09-18 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
EP1837407A2 (en) * | 2001-09-24 | 2007-09-26 | Intel Corporation | Nucleic acid sequencing by monitoring of uptake of precursors during molecular replication, with molecule dispenser |
US7302146B2 (en) | 2004-09-17 | 2007-11-27 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
US7427673B2 (en) | 2001-12-04 | 2008-09-23 | Illumina Cambridge Limited | Labelled nucleotides |
US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
US7541444B2 (en) | 2002-08-23 | 2009-06-02 | Illumina Cambridge Limited | Modified nucleotides |
US7592435B2 (en) | 2005-08-19 | 2009-09-22 | Illumina Cambridge Limited | Modified nucleosides and nucleotides and uses thereof |
US7645596B2 (en) | 1998-05-01 | 2010-01-12 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
US7772384B2 (en) | 2001-12-04 | 2010-08-10 | Illumina Cambridge Limited | Labelled nucleotides |
US7897345B2 (en) | 2003-11-12 | 2011-03-01 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US7981604B2 (en) | 2004-02-19 | 2011-07-19 | California Institute Of Technology | Methods and kits for analyzing polynucleotide sequences |
US8114591B2 (en) | 2001-03-09 | 2012-02-14 | Dna Electronics Ltd. | Sensing apparatus and method |
US8192961B2 (en) | 1998-12-14 | 2012-06-05 | Pacific Biosciences Of California, Inc. | System and methods for nucleic acid sequencing of single molecules by polymerase synthesis |
US8263364B2 (en) | 1998-05-01 | 2012-09-11 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US8399188B2 (en) | 2006-09-28 | 2013-03-19 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
US8911972B2 (en) | 2009-12-16 | 2014-12-16 | Pacific Biosciences Of California, Inc. | Sequencing methods using enzyme conformation |
US8993230B2 (en) | 2008-12-04 | 2015-03-31 | Pacific Biosciences of Californ, Inc. | Asynchronous sequencing of biological polymers |
US9115163B2 (en) | 2007-10-19 | 2015-08-25 | The Trustees Of Columbia University In The City Of New York | DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
US9127314B2 (en) | 2002-08-23 | 2015-09-08 | Illumina Cambridge Limited | Labelled nucleotides |
US9133511B2 (en) | 2000-10-06 | 2015-09-15 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9175342B2 (en) | 2007-10-19 | 2015-11-03 | The Trustees Of Columbia University In The City Of New York | Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis |
US9243284B2 (en) | 2000-12-01 | 2016-01-26 | Life Technologies Corporation | Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis |
US9528151B2 (en) | 2006-12-01 | 2016-12-27 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
US20170166961A1 (en) | 2013-03-15 | 2017-06-15 | Illumina Cambridge Limited | Modified nucleosides or nucleotides |
US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9951385B1 (en) | 2017-04-25 | 2018-04-24 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
US10077470B2 (en) | 2015-07-21 | 2018-09-18 | Omniome, Inc. | Nucleic acid sequencing methods and systems |
US10161003B2 (en) | 2017-04-25 | 2018-12-25 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
US10246744B2 (en) | 2016-08-15 | 2019-04-02 | Omniome, Inc. | Method and system for sequencing nucleic acids |
US10294514B2 (en) | 2016-04-29 | 2019-05-21 | Omniome, Inc. | Sequencing method employing ternary complex destabilization to identify cognate nucleotides |
US10428378B2 (en) | 2016-08-15 | 2019-10-01 | Omniome, Inc. | Sequencing method for rapid identification and processing of cognate nucleotide pairs |
US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
US10975427B2 (en) | 2017-01-20 | 2021-04-13 | Omniome, Inc. | Process for cognate nucleotide detection in a nucleic acid sequencing workflow |
US10995111B2 (en) | 2003-08-22 | 2021-05-04 | Illumina Cambridge Limited | Labelled nucleotides |
US11193166B2 (en) | 2017-10-19 | 2021-12-07 | Omniome, Inc. | Simultaneous background reduction and complex stabilization in binding assay workflows |
US11248254B2 (en) | 2016-12-30 | 2022-02-15 | Omniome, Inc. | Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides |
WO2022101400A1 (en) * | 2020-11-16 | 2022-05-19 | Illumina, Inc. | Incorporation and imaging mixes |
US11705217B2 (en) | 2008-03-28 | 2023-07-18 | Pacific Biosciences Of California, Inc. | Sequencing using concatemers of copies of sense and antisense strands |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002171999A (en) * | 2000-12-08 | 2002-06-18 | Olympus Optical Co Ltd | Method and apparatus for assaying nucleic acid amplification reaction |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0223618A2 (en) * | 1985-07-18 | 1987-05-27 | New York Medical College | Automatable process for nucleotide sequencing |
EP0233053A2 (en) * | 1986-02-07 | 1987-08-19 | Applied Biosystems, Inc. | Method of detecting electrophoretically separated oligonucleotides |
US4770992A (en) * | 1985-11-27 | 1988-09-13 | Den Engh Gerrit J Van | Detection of specific DNA sequences by flow cytometry |
WO1989003432A1 (en) * | 1987-10-07 | 1989-04-20 | United States Department Of Energy | Method for rapid base sequencing in dna and rna |
-
1989
- 1989-05-11 GB GB898910880A patent/GB8910880D0/en active Pending
-
1990
- 1990-05-11 JP JP2507195A patent/JPH04505251A/en active Pending
- 1990-05-11 CA CA002045505A patent/CA2045505A1/en not_active Abandoned
- 1990-05-11 WO PCT/GB1990/000726 patent/WO1990013666A1/en not_active Application Discontinuation
- 1990-05-11 EP EP90907267A patent/EP0471732A1/en not_active Withdrawn
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0223618A2 (en) * | 1985-07-18 | 1987-05-27 | New York Medical College | Automatable process for nucleotide sequencing |
US4770992A (en) * | 1985-11-27 | 1988-09-13 | Den Engh Gerrit J Van | Detection of specific DNA sequences by flow cytometry |
EP0233053A2 (en) * | 1986-02-07 | 1987-08-19 | Applied Biosystems, Inc. | Method of detecting electrophoretically separated oligonucleotides |
WO1989003432A1 (en) * | 1987-10-07 | 1989-04-20 | United States Department Of Energy | Method for rapid base sequencing in dna and rna |
Cited By (243)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5902723A (en) * | 1989-06-07 | 1999-05-11 | Dower; William J. | Analysis of surface immobilized polymers utilizing microfluorescence detection |
US5547839A (en) * | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
WO1991006678A1 (en) * | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
US7056666B2 (en) | 1990-12-06 | 2006-06-06 | Affymetrix, Inc. | Analysis of surface immobilized polymers utilizing microfluorescence detection |
US5750337A (en) * | 1991-09-16 | 1998-05-12 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Methods for detecting nucleic acid sequences using evanescent wave detection |
WO1993006241A1 (en) * | 1991-09-16 | 1993-04-01 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Gene probe biosensor method |
GB2274710A (en) * | 1991-09-16 | 1994-08-03 | Secr Defence | Gene probe biosensor method |
GB2274710B (en) * | 1991-09-16 | 1995-09-06 | Secr Defence | Detection of nucleic acid sequences in a sample using an oligonucleotide immobilised on a waveguide |
US6087095A (en) * | 1992-04-22 | 2000-07-11 | Medical Research Council | DNA sequencing method |
WO1993021340A1 (en) * | 1992-04-22 | 1993-10-28 | Medical Research Council | Dna sequencing method |
US7001722B1 (en) | 1993-06-22 | 2006-02-21 | Baylor College Of Medicine | Parallel primer extension approach to nucleic acid sequence analysis |
US6153379A (en) * | 1993-06-22 | 2000-11-28 | Baylor College Of Medicine | Parallel primer extension approach to nucleic acid sequence analysis |
WO1997004129A1 (en) * | 1995-07-14 | 1997-02-06 | Biacore Ab | Method for nucleic acid sequencing |
US6686158B2 (en) | 1995-11-30 | 2004-02-03 | Pharma Seg, Inc. | Electronically-indexed solid-phase assay for biomolecules |
US5736332A (en) * | 1995-11-30 | 1998-04-07 | Mandecki; Wlodek | Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders |
US6361950B1 (en) | 1995-11-30 | 2002-03-26 | Pharmaseq, Inc. | Multiplex assay for nucleic acids employing transponders |
US6051377A (en) * | 1995-11-30 | 2000-04-18 | Pharmaseq, Inc. | Multiplex assay for nucleic acids employing transponders |
US5641634A (en) * | 1995-11-30 | 1997-06-24 | Mandecki; Wlodek | Electronically-indexed solid-phase assay for biomolecules |
US6376187B1 (en) | 1995-11-30 | 2002-04-23 | Pharmaseq, Inc. | Electronically-indexed solid-phase assay for biomolecules |
US6387623B1 (en) | 1995-11-30 | 2002-05-14 | Pharmaseq | Screening of drugs from chemical combinatorial libraries employing transponders |
US6046003A (en) * | 1995-11-30 | 2000-04-04 | Pharmaseq, Inc. | Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders |
US7247434B2 (en) | 1996-01-23 | 2007-07-24 | Operon Biotechnologies, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
US6613508B1 (en) | 1996-01-23 | 2003-09-02 | Qiagen Genomics, Inc. | Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques |
US7052846B2 (en) | 1996-01-23 | 2006-05-30 | Operon Biotechnologies, Inc. | Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques |
US6027890A (en) * | 1996-01-23 | 2000-02-22 | Rapigene, Inc. | Methods and compositions for enhancing sensitivity in the analysis of biological-based assays |
US6312893B1 (en) | 1996-01-23 | 2001-11-06 | Qiagen Genomics, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
WO1997027331A3 (en) * | 1996-01-23 | 1998-04-02 | Darwin Molecular Corp | Methods and compositions for determining the sequence of nucleic acid molecules |
US6623928B2 (en) | 1996-01-23 | 2003-09-23 | Qiagen Genomics, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
US7642344B2 (en) | 1996-01-23 | 2010-01-05 | Operon Biotechnologies, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
US6815212B2 (en) | 1996-01-23 | 2004-11-09 | Qiagen Genomics, Inc. | Methods and compositions for enhancing sensitivity in the analysis of biological-based assays |
WO1997027331A2 (en) * | 1996-01-23 | 1997-07-31 | Rapigene, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
US5908755A (en) * | 1996-06-14 | 1999-06-01 | Sarnoff Corporation | Sequential step method for sequencing and identifying polynucleotides |
AU740238B2 (en) * | 1996-06-14 | 2001-11-01 | Sarnoff Corporation | Method for polynucleotide sequencing |
WO1997047761A1 (en) * | 1996-06-14 | 1997-12-18 | Sarnoff Corporation | Method for polynucleotide sequencing |
US6136543A (en) * | 1997-01-31 | 2000-10-24 | Hitachi, Ltd. | Method for determining nucleic acids base sequence and apparatus therefor |
US6197557B1 (en) | 1997-03-05 | 2001-03-06 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
US6762022B2 (en) | 1997-03-05 | 2004-07-13 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
US6117634A (en) * | 1997-03-05 | 2000-09-12 | The Reagents Of The University Of Michigan | Nucleic acid sequencing and mapping |
US6537757B1 (en) | 1997-03-05 | 2003-03-25 | The Regents Of The University Of Michigan | Nucleic acid sequencing and mapping |
WO1998039485A3 (en) * | 1997-03-05 | 1999-02-25 | Univ Michigan | Compositions and methods for analysis of nucleic acids |
WO1998039485A2 (en) * | 1997-03-05 | 1998-09-11 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
US5981166A (en) * | 1997-04-23 | 1999-11-09 | Pharmaseq, Inc. | Screening of soluble chemical compounds for their pharmacological properties utilizing transponders |
US6248521B1 (en) | 1997-07-22 | 2001-06-19 | Qiagen Genomics, Inc. | Amplification and other enzymatic reactions performed on nucleic acid arrays |
WO1999005321A1 (en) * | 1997-07-22 | 1999-02-04 | Rapigene, Inc. | Amplification and other enzymatic reactions performed on nucleic acid arrays |
WO1999005320A1 (en) * | 1997-07-22 | 1999-02-04 | Rapigene, Inc. | Multiple functionalities within an array element and uses thereof |
US6365349B1 (en) | 1997-07-22 | 2002-04-02 | Qiagen Genomics, Inc. | Apparatus and methods for arraying solution onto a solid support |
AU742599B2 (en) * | 1997-07-22 | 2002-01-10 | Qiagen Genomics, Inc. | Multiple functionalities within an array element and uses thereof |
EP1229133A3 (en) * | 1997-07-28 | 2004-10-13 | Medical Biosystems Ltd. | Nucleic acid sequence analysis |
EP1229133A2 (en) * | 1997-07-28 | 2002-08-07 | Medical Biosystems Ltd. | Nucleic acid sequence analysis |
WO1999005315A2 (en) * | 1997-07-28 | 1999-02-04 | Medical Biosystems Ltd. | Nucleic acid sequence analysis |
WO1999005315A3 (en) * | 1997-07-28 | 1999-04-22 | Medical Biosystems Ltd | Nucleic acid sequence analysis |
US7008766B1 (en) | 1997-07-28 | 2006-03-07 | Medical Biosystems, Ltd. | Nucleic acid sequence analysis |
EP2267164A3 (en) * | 1997-07-28 | 2012-08-15 | Gen-Probe Incorporated | Nucleic acid sequence analysis |
EP2267165A3 (en) * | 1997-07-28 | 2012-10-10 | Gen-Probe Incorporated | Nucleic acid sequence analysis |
EP1017848B2 (en) † | 1997-07-28 | 2012-02-22 | Gen-Probe Incorporated | Nucleic acid sequence analysis |
US7785790B1 (en) | 1997-10-10 | 2010-08-31 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
US6511803B1 (en) * | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
WO1999054497A1 (en) * | 1998-04-22 | 1999-10-28 | Stefan Seeger | Method and device for determining the concentration, adsorption and binding kinetics and equilibrium and binding constants of molecules by luminescence measurements |
US7037687B2 (en) | 1998-05-01 | 2006-05-02 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US9725764B2 (en) | 1998-05-01 | 2017-08-08 | Life Technologies Corporation | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US10214774B2 (en) | 1998-05-01 | 2019-02-26 | Life Technologies Corporation | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US8263365B2 (en) | 1998-05-01 | 2012-09-11 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US10208341B2 (en) | 1998-05-01 | 2019-02-19 | Life Technologies Corporation | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US8263364B2 (en) | 1998-05-01 | 2012-09-11 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
WO1999057321A1 (en) * | 1998-05-01 | 1999-11-11 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and dna molecules |
US9458500B2 (en) | 1998-05-01 | 2016-10-04 | Life Technologies Corporation | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US9957561B2 (en) | 1998-05-01 | 2018-05-01 | Life Technologies Corporation | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US7645596B2 (en) | 1998-05-01 | 2010-01-12 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US9212393B2 (en) | 1998-05-01 | 2015-12-15 | Life Technologies Corporation | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US9096898B2 (en) | 1998-05-01 | 2015-08-04 | Life Technologies Corporation | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US9540689B2 (en) | 1998-05-01 | 2017-01-10 | Life Technologies Corporation | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
US7232656B2 (en) | 1998-07-30 | 2007-06-19 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
US7270958B2 (en) | 1998-09-10 | 2007-09-18 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
WO2000018956A1 (en) * | 1998-09-30 | 2000-04-06 | Molecular Machines & Industries Gmbh | Method for dna- or rna-sequencing |
US9845501B2 (en) | 1998-12-14 | 2017-12-19 | Pacific of Biosciences of California, Inc. | System and methods for nucleic acid sequencing of single molecules by polymerase synthesis |
US8980584B2 (en) | 1998-12-14 | 2015-03-17 | Pacific Biosciences Of California, Inc. | System and methods for nucleic acid sequencing of single molecules by polymerase synthesis |
US8192961B2 (en) | 1998-12-14 | 2012-06-05 | Pacific Biosciences Of California, Inc. | System and methods for nucleic acid sequencing of single molecules by polymerase synthesis |
EP1163369A1 (en) * | 1999-02-23 | 2001-12-19 | Caliper Technologies Corporation | Sequencing by incorporation |
US6613513B1 (en) | 1999-02-23 | 2003-09-02 | Caliper Technologies Corp. | Sequencing by incorporation |
US7105300B2 (en) | 1999-02-23 | 2006-09-12 | Caliper Life Sciences, Inc. | Sequencing by incorporation |
EP1163369A4 (en) * | 1999-02-23 | 2002-08-28 | Caliper Techn Corp | Sequencing by incorporation |
US7566538B2 (en) | 1999-02-23 | 2009-07-28 | Caliper Lifesciences Inc. | Sequencing by incorporation |
US7344865B2 (en) | 1999-02-23 | 2008-03-18 | Caliper Life Sciences, Inc. | Sequencing by incorporation |
EP2177627A1 (en) * | 1999-02-23 | 2010-04-21 | Caliper Life Sciences, Inc. | Manipulation of microparticles in microfluidic systems |
US7270951B1 (en) | 1999-03-10 | 2007-09-18 | Asm Scientific, Inc. | Method for direct nucleic acid sequencing |
EP1961826A3 (en) * | 1999-03-10 | 2008-09-17 | ASM Scientific, Inc. | A method for direct nucleic acid sequencing |
WO2000053805A1 (en) * | 1999-03-10 | 2000-09-14 | Asm Scientific, Inc. | A method for direct nucleic acid sequencing |
WO2000058507A1 (en) * | 1999-03-30 | 2000-10-05 | Solexa Ltd. | Polynucleotide sequencing |
WO2000060114A2 (en) * | 1999-04-06 | 2000-10-12 | Medical Biosystems Ltd. | Polynucleotide sequencing using a helicase |
WO2000060114A3 (en) * | 1999-04-06 | 2002-01-31 | Medical Biosystems Ltd | Polynucleotide sequencing using a helicase |
US6573047B1 (en) | 1999-04-13 | 2003-06-03 | Dna Sciences, Inc. | Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation |
US7056676B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US7052847B2 (en) | 1999-05-19 | 2006-05-30 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US7361466B2 (en) | 1999-05-19 | 2008-04-22 | Cornell Research Foundation, Inc. | Nucleic acid analysis using terminal-phosphate-labeled nucleotides |
EP1179085A4 (en) * | 1999-05-19 | 2004-11-10 | Cornell Res Foundation Inc | Method for sequencing nucleic acid molecules |
EP1681356A1 (en) * | 1999-05-19 | 2006-07-19 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US7416844B2 (en) | 1999-05-19 | 2008-08-26 | Cornell Research Foundation, Inc. | Composition for nucleic acid sequencing |
US7485424B2 (en) | 1999-05-19 | 2009-02-03 | Cornell Research Foundation, Inc. | Labeled nucleotide phosphate (NP) probes |
US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US7943305B2 (en) | 1999-05-19 | 2011-05-17 | Cornell Research Foundation | High speed nucleic acid sequencing |
EP1179085A1 (en) * | 1999-05-19 | 2002-02-13 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US7033764B2 (en) | 1999-05-19 | 2006-04-25 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US7943307B2 (en) | 1999-05-19 | 2011-05-17 | Cornell Research Foundation | Methods for analyzing nucleic acid sequences |
US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
US7501245B2 (en) | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
US6242193B1 (en) | 1999-07-30 | 2001-06-05 | Hitachi, Ltd. | Apparatus for determining base sequence of nucleic acid |
WO2001025480A2 (en) * | 1999-10-06 | 2001-04-12 | Medical Biosystems Ltd. | Dna sequencing method |
WO2001025480A3 (en) * | 1999-10-06 | 2002-05-16 | Medical Biosystems Ltd | Dna sequencing method |
US6908736B1 (en) | 1999-10-06 | 2005-06-21 | Medical Biosystems, Ltd. | DNA sequencing method |
US7939264B1 (en) | 1999-10-06 | 2011-05-10 | Gen-Probe Incorporated | DNA sequencing method |
WO2001032930A1 (en) * | 1999-11-04 | 2001-05-10 | California Institute Of Technology | Methods and apparatuses for analyzing polynucleotide sequences |
US6458544B1 (en) | 1999-12-02 | 2002-10-01 | Dna Sciences, Inc. | Methods for determining single nucleotide variations and genotyping |
WO2001057248A3 (en) * | 2000-02-01 | 2002-02-14 | Solexa Ltd | Polynucleotide arrays and their use in sequencing |
WO2001057248A2 (en) * | 2000-02-01 | 2001-08-09 | Solexa Ltd. | Polynucleotide arrays and their use in sequencing |
WO2001057249A1 (en) * | 2000-02-02 | 2001-08-09 | Solexa Ltd. | Synthesis of spatially addressed molecular arrays |
US7384737B2 (en) | 2000-02-02 | 2008-06-10 | Solexa Limited | Synthesis of spatially addressed molecular arrays |
EP1182267A4 (en) * | 2000-03-30 | 2004-09-15 | Toyota Motor Co Ltd | Method of determining base sequence of single nucleic acid molecule |
WO2001073121A1 (en) * | 2000-03-30 | 2001-10-04 | Toyota Jidosha Kabushiki Kaisha | Method of determining base sequence of single nucleic acid molecule |
EP1182267A1 (en) * | 2000-03-30 | 2002-02-27 | Toyota Jidosha Kabushiki Kaisha | Method of determining base sequence of single nucleic acid molecule |
US7223568B2 (en) | 2000-03-30 | 2007-05-29 | Toyota Jidosha Kabushiki Kaisha | Methods for determining nucleotide sequences of single nucleic acid molecules |
US6355433B1 (en) | 2000-06-02 | 2002-03-12 | Dna Sciences, Inc. | Determination of nucleotide sequence variations through limited primer extension |
US7056670B2 (en) | 2000-07-05 | 2006-06-06 | Ge Healthcare Uk Limited | Sequencing method |
WO2002002813A3 (en) * | 2000-07-05 | 2003-03-13 | Amersham Biosiences Uk Ltd | Sequencing method |
WO2002002813A2 (en) * | 2000-07-05 | 2002-01-10 | Amersham Biosiences Uk Ltd. | Sequencing method |
US10633700B2 (en) | 2000-10-06 | 2020-04-28 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10428380B2 (en) | 2000-10-06 | 2019-10-01 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10669582B2 (en) | 2000-10-06 | 2020-06-02 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9868985B2 (en) | 2000-10-06 | 2018-01-16 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10435742B2 (en) | 2000-10-06 | 2019-10-08 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9133511B2 (en) | 2000-10-06 | 2015-09-15 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10570446B2 (en) | 2000-10-06 | 2020-02-25 | The Trustee Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10669577B2 (en) | 2000-10-06 | 2020-06-02 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9725480B2 (en) | 2000-10-06 | 2017-08-08 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9718852B2 (en) | 2000-10-06 | 2017-08-01 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10407459B2 (en) | 2000-10-06 | 2019-09-10 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10577652B2 (en) | 2000-10-06 | 2020-03-03 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10662472B2 (en) | 2000-10-06 | 2020-05-26 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9719139B2 (en) | 2000-10-06 | 2017-08-01 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10407458B2 (en) | 2000-10-06 | 2019-09-10 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10648028B2 (en) | 2000-10-06 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US10457984B2 (en) | 2000-10-06 | 2019-10-29 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9243284B2 (en) | 2000-12-01 | 2016-01-26 | Life Technologies Corporation | Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis |
US8114591B2 (en) | 2001-03-09 | 2012-02-14 | Dna Electronics Ltd. | Sensing apparatus and method |
GB2389424B (en) * | 2001-03-09 | 2004-11-24 | Christofer Toumazou | Sensing apparatus and method |
US7686929B2 (en) | 2001-03-09 | 2010-03-30 | Dna Electronics Limited | Sensing apparatus and method |
US7888015B2 (en) | 2001-03-09 | 2011-02-15 | Dna Electronics Ltd. | qPCR using solid-state sensing |
WO2003073088A2 (en) * | 2001-03-09 | 2003-09-04 | Christofer Toumazou | Apparatus and method for the detection of localised fluctuactions of ionic charge by ion sensitive field effect transistors during a chemical reaction |
WO2003073088A3 (en) * | 2001-03-09 | 2003-11-20 | Christofer Toumazou | Apparatus and method for the detection of localised fluctuactions of ionic charge by ion sensitive field effect transistors during a chemical reaction |
US8685228B2 (en) | 2001-03-09 | 2014-04-01 | Dna Electronics Limited | Sensing apparatus and method |
US8698211B2 (en) | 2001-03-09 | 2014-04-15 | Dna Electronics Ltd. | Sensing apparatus and method |
US6653082B2 (en) | 2001-05-17 | 2003-11-25 | Baylor College Of Medicine | Substrate-bound cleavage assay for nucleic acid analysis |
EP1837407A2 (en) * | 2001-09-24 | 2007-09-26 | Intel Corporation | Nucleic acid sequencing by monitoring of uptake of precursors during molecular replication, with molecule dispenser |
EP1837407A3 (en) * | 2001-09-24 | 2007-11-21 | Intel Corporation | Nucleic acid sequencing by monitoring of uptake of precursors during molecular replication, with molecule dispenser |
US7566537B2 (en) | 2001-12-04 | 2009-07-28 | Illumina Cambridge Limited | Labelled nucleotides |
US8158346B2 (en) | 2001-12-04 | 2012-04-17 | Illumina Cambridge Limited | Labelled nucleotides |
US7427673B2 (en) | 2001-12-04 | 2008-09-23 | Illumina Cambridge Limited | Labelled nucleotides |
US10480025B2 (en) | 2001-12-04 | 2019-11-19 | Illumina Cambridge Limited | Labelled nucleotides |
US9605310B2 (en) | 2001-12-04 | 2017-03-28 | Illumina Cambridge Limited | Labelled nucleotides |
US7785796B2 (en) | 2001-12-04 | 2010-08-31 | Illumina Cambridge Limited | Labelled nucleotides |
US8148064B2 (en) | 2001-12-04 | 2012-04-03 | Illumina Cambridge Limited | Labelled nucleotides |
US7772384B2 (en) | 2001-12-04 | 2010-08-10 | Illumina Cambridge Limited | Labelled nucleotides |
US10519496B2 (en) | 2001-12-04 | 2019-12-31 | Illumina Cambridge Limited | Labelled nucleotides |
US9121062B2 (en) | 2001-12-04 | 2015-09-01 | Illumina Cambridge Limited | Labelled nucleotides |
US9410200B2 (en) | 2001-12-04 | 2016-08-09 | Illumina Cambridge Limited | Labelled nucleotides |
US9388463B2 (en) | 2001-12-04 | 2016-07-12 | Illumina Cambridge Limited | Labelled nucleotides |
US8394586B2 (en) | 2001-12-04 | 2013-03-12 | Illumina Cambridge Limited | Labelled nucleotides |
US7541444B2 (en) | 2002-08-23 | 2009-06-02 | Illumina Cambridge Limited | Modified nucleotides |
US9388464B2 (en) | 2002-08-23 | 2016-07-12 | Illumina Cambridge Limited | Modified nucleotides |
US9127314B2 (en) | 2002-08-23 | 2015-09-08 | Illumina Cambridge Limited | Labelled nucleotides |
US9410199B2 (en) | 2002-08-23 | 2016-08-09 | Illumina Cambridge Limited | Labelled nucleotides |
US8071739B2 (en) | 2002-08-23 | 2011-12-06 | Illumina Cambridge Limited | Modified nucleotides |
US9121060B2 (en) | 2002-08-23 | 2015-09-01 | Illumina Cambridge Limited | Modified nucleotides |
US10513731B2 (en) | 2002-08-23 | 2019-12-24 | Illumina Cambridge Limited | Modified nucleotides |
US11008359B2 (en) | 2002-08-23 | 2021-05-18 | Illumina Cambridge Limited | Labelled nucleotides |
US10487102B2 (en) | 2002-08-23 | 2019-11-26 | Illumina Cambridge Limited | Labelled nucleotides |
WO2004029294A1 (en) * | 2002-09-27 | 2004-04-08 | Biotage Ab | New sequencing method for sequencing rna molecules |
US7771973B2 (en) | 2002-12-23 | 2010-08-10 | Illumina Cambridge Limited | Modified nucleotides |
US8597881B2 (en) | 2002-12-23 | 2013-12-03 | Illumina Cambridge Limited | Modified nucleotides |
WO2004072294A2 (en) * | 2003-02-12 | 2004-08-26 | Genizon Svenska Ab | Methods and means for nucleic acid sequencing |
WO2004072294A3 (en) * | 2003-02-12 | 2005-03-10 | Global Genomics Ab | Methods and means for nucleic acid sequencing |
WO2004106545A1 (en) * | 2003-05-28 | 2004-12-09 | Innogenetics N.V. | Methods for enhanced detection using surface sensitive techniques. |
US11028116B2 (en) | 2003-08-22 | 2021-06-08 | Illumina Cambridge Limited | Labelled nucleotides |
US11028115B2 (en) | 2003-08-22 | 2021-06-08 | Illumina Cambridge Limited | Labelled nucleotides |
US10995111B2 (en) | 2003-08-22 | 2021-05-04 | Illumina Cambridge Limited | Labelled nucleotides |
US9657344B2 (en) | 2003-11-12 | 2017-05-23 | Fluidigm Corporation | Short cycle methods for sequencing polynucleotides |
US7897345B2 (en) | 2003-11-12 | 2011-03-01 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US9012144B2 (en) | 2003-11-12 | 2015-04-21 | Fluidigm Corporation | Short cycle methods for sequencing polynucleotides |
US7981604B2 (en) | 2004-02-19 | 2011-07-19 | California Institute Of Technology | Methods and kits for analyzing polynucleotide sequences |
WO2005118871A1 (en) * | 2004-05-28 | 2005-12-15 | The Arizona Board Of Regents | Surface plasmon resonance sensor for detecting changes in polynucleotides mass |
US7906284B2 (en) | 2004-09-17 | 2011-03-15 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
US7302146B2 (en) | 2004-09-17 | 2007-11-27 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
US9588051B2 (en) | 2004-09-17 | 2017-03-07 | Pacific Biosciences Of California, Inc. | Apparatus and method for performing nucleic acid analysis |
US9709503B2 (en) | 2004-09-17 | 2017-07-18 | Pacific Biosciences Of California, Inc. | Apparatus and method for performing nucleic acid analysis |
US7313308B2 (en) | 2004-09-17 | 2007-12-25 | Pacific Biosciences Of California, Inc. | Optical analysis of molecules |
US7315019B2 (en) | 2004-09-17 | 2008-01-01 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
US7170050B2 (en) | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
US7476503B2 (en) | 2004-09-17 | 2009-01-13 | Pacific Biosciences Of California, Inc. | Apparatus and method for performing nucleic acid analysis |
US8212015B2 (en) | 2005-08-19 | 2012-07-03 | Illumina Cambridge Limited | Modified nucleosides and nucleotides and uses thereof |
US7816503B2 (en) | 2005-08-19 | 2010-10-19 | Illumina Cambridge Limited | Modified nucleosides and nucleotides and uses thereof |
US7592435B2 (en) | 2005-08-19 | 2009-09-22 | Illumina Cambridge Limited | Modified nucleosides and nucleotides and uses thereof |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
US9868978B2 (en) | 2005-08-26 | 2018-01-16 | Fluidigm Corporation | Single molecule sequencing of captured nucleic acids |
US9051612B2 (en) | 2006-09-28 | 2015-06-09 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
US8808988B2 (en) | 2006-09-28 | 2014-08-19 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
US8399188B2 (en) | 2006-09-28 | 2013-03-19 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
US9469873B2 (en) | 2006-09-28 | 2016-10-18 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
US9528151B2 (en) | 2006-12-01 | 2016-12-27 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
US11939631B2 (en) | 2006-12-01 | 2024-03-26 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
US11098353B2 (en) | 2006-12-01 | 2021-08-24 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
US9115163B2 (en) | 2007-10-19 | 2015-08-25 | The Trustees Of Columbia University In The City Of New York | DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
US10144961B2 (en) | 2007-10-19 | 2018-12-04 | The Trustees Of Columbia University In The City Of New York | Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis |
US11208691B2 (en) | 2007-10-19 | 2021-12-28 | The Trustees Of Columbia University In The City Of New York | Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis |
US11242561B2 (en) | 2007-10-19 | 2022-02-08 | The Trustees Of Columbia University In The City Of New York | DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
US9670539B2 (en) | 2007-10-19 | 2017-06-06 | The Trustees Of Columbia University In The City Of New York | Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis |
US9175342B2 (en) | 2007-10-19 | 2015-11-03 | The Trustees Of Columbia University In The City Of New York | Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis |
US10260094B2 (en) | 2007-10-19 | 2019-04-16 | The Trustees Of Columbia University In The City Of New York | DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
US11705217B2 (en) | 2008-03-28 | 2023-07-18 | Pacific Biosciences Of California, Inc. | Sequencing using concatemers of copies of sense and antisense strands |
US8993230B2 (en) | 2008-12-04 | 2015-03-31 | Pacific Biosciences of Californ, Inc. | Asynchronous sequencing of biological polymers |
US8911972B2 (en) | 2009-12-16 | 2014-12-16 | Pacific Biosciences Of California, Inc. | Sequencing methods using enzyme conformation |
US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
US10982277B2 (en) | 2013-03-15 | 2021-04-20 | Illumina Cambridge Limited | Modified nucleosides or nucleotides |
US20170166961A1 (en) | 2013-03-15 | 2017-06-15 | Illumina Cambridge Limited | Modified nucleosides or nucleotides |
US10407721B2 (en) | 2013-03-15 | 2019-09-10 | Illumina Cambridge Limited | Modified nucleosides or nucleotides |
US10077470B2 (en) | 2015-07-21 | 2018-09-18 | Omniome, Inc. | Nucleic acid sequencing methods and systems |
US11499185B2 (en) | 2015-07-21 | 2022-11-15 | Pacific Biosciences Of California, Inc. | Nucleic acid sequencing methods and systems |
US11203778B2 (en) | 2016-04-29 | 2021-12-21 | Omniome, Inc. | Sequencing method employing ternary complex destabilization to identify cognate nucleotides |
US10633692B2 (en) | 2016-04-29 | 2020-04-28 | Omniome, Inc. | Sequencing method employing ternary complex destabilization to identify cognate nucleotides |
US10294514B2 (en) | 2016-04-29 | 2019-05-21 | Omniome, Inc. | Sequencing method employing ternary complex destabilization to identify cognate nucleotides |
US11203779B2 (en) | 2016-08-15 | 2021-12-21 | Omnionie, Inc. | Sequencing method for rapid identification and processing of cognate nucleotide pairs |
US11168364B2 (en) | 2016-08-15 | 2021-11-09 | Omniome, Inc. | Method and system for sequencing nucleic acids |
US10443098B2 (en) | 2016-08-15 | 2019-10-15 | Omniome, Inc. | Method and system for sequencing nucleic acids |
US10246744B2 (en) | 2016-08-15 | 2019-04-02 | Omniome, Inc. | Method and system for sequencing nucleic acids |
US10428378B2 (en) | 2016-08-15 | 2019-10-01 | Omniome, Inc. | Sequencing method for rapid identification and processing of cognate nucleotide pairs |
US11248254B2 (en) | 2016-12-30 | 2022-02-15 | Omniome, Inc. | Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides |
US10975427B2 (en) | 2017-01-20 | 2021-04-13 | Omniome, Inc. | Process for cognate nucleotide detection in a nucleic acid sequencing workflow |
US10655176B2 (en) | 2017-04-25 | 2020-05-19 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
US9951385B1 (en) | 2017-04-25 | 2018-04-24 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
US10161003B2 (en) | 2017-04-25 | 2018-12-25 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
US11447823B2 (en) | 2017-04-25 | 2022-09-20 | Pacific Biosciences Of California, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
US11193166B2 (en) | 2017-10-19 | 2021-12-07 | Omniome, Inc. | Simultaneous background reduction and complex stabilization in binding assay workflows |
WO2022101400A1 (en) * | 2020-11-16 | 2022-05-19 | Illumina, Inc. | Incorporation and imaging mixes |
Also Published As
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CA2045505A1 (en) | 1990-11-12 |
GB8910880D0 (en) | 1989-06-28 |
EP0471732A1 (en) | 1992-02-26 |
JPH04505251A (en) | 1992-09-17 |
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