AU707243B2 - Mutant luciferases - Google Patents

Mutant luciferases Download PDF

Info

Publication number
AU707243B2
AU707243B2 AU43973/96A AU4397396A AU707243B2 AU 707243 B2 AU707243 B2 AU 707243B2 AU 43973/96 A AU43973/96 A AU 43973/96A AU 4397396 A AU4397396 A AU 4397396A AU 707243 B2 AU707243 B2 AU 707243B2
Authority
AU
Australia
Prior art keywords
amino acid
luciola
protein
luciferase
residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU43973/96A
Other languages
English (en)
Other versions
AU4397396A (en
Inventor
Christopher Robin Lowe
James Augustus Henry Murray
David James Squirrell
Peter John White
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
SECR DEFENCE
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9501172.2A external-priority patent/GB9501172D0/en
Priority claimed from GBGB9508301.0A external-priority patent/GB9508301D0/en
Application filed by SECR DEFENCE, UK Secretary of State for Defence filed Critical SECR DEFENCE
Publication of AU4397396A publication Critical patent/AU4397396A/en
Application granted granted Critical
Publication of AU707243B2 publication Critical patent/AU707243B2/en
Assigned to 3M INNOVATIVE PROPERTIES COMPANY reassignment 3M INNOVATIVE PROPERTIES COMPANY Alteration of Name(s) in Register under S187 Assignors: SECRETARY OF STATE FOR DEFENCE, THE
Assigned to PROMEGA CORPORATION reassignment PROMEGA CORPORATION Alteration of Name(s) in Register under S187 Assignors: 3M INNOVATIVE PROPERTIES COMPANY
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AU43973/96A 1995-01-20 1996-01-19 Mutant luciferases Ceased AU707243B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB9501172 1995-01-20
GBGB9501172.2A GB9501172D0 (en) 1995-01-20 1995-01-20 Luciferases
GB9508301 1995-04-24
GBGB9508301.0A GB9508301D0 (en) 1995-04-24 1995-04-24 Luciferases
PCT/GB1996/000099 WO1996022376A1 (en) 1995-01-20 1996-01-19 Mutant luciferases

Publications (2)

Publication Number Publication Date
AU4397396A AU4397396A (en) 1996-08-07
AU707243B2 true AU707243B2 (en) 1999-07-08

Family

ID=26306352

Family Applications (1)

Application Number Title Priority Date Filing Date
AU43973/96A Ceased AU707243B2 (en) 1995-01-20 1996-01-19 Mutant luciferases

Country Status (10)

Country Link
US (1) US6171808B1 (enExample)
EP (1) EP0804587B1 (enExample)
JP (1) JP4342608B2 (enExample)
AU (1) AU707243B2 (enExample)
CA (1) CA2210354C (enExample)
DE (1) DE69633171T2 (enExample)
DK (1) DK0804587T3 (enExample)
IN (1) IN186115B (enExample)
NO (1) NO322134B1 (enExample)
WO (1) WO1996022376A1 (enExample)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
DK1707621T3 (da) * 1997-07-08 2010-03-29 Kikkoman Corp Muteret bioluminescerende protein og fremgangsmåde til fremstilling af det muterede bioluminiscerende protein
EP1707621B1 (en) 1997-07-08 2009-11-18 Kikkoman Corporation Mutant bioluminescent protein and process for producing the mutant bioluminescent protein
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
EP1015601B1 (en) * 1997-09-19 2015-01-07 Promega Corporation Thermostable luciferases and methods of production
GB9722481D0 (en) * 1997-10-25 1997-12-24 Secr Defence Expression system
GB9823468D0 (en) * 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP5154724B2 (ja) * 1999-10-26 2013-02-27 プロメガ コーポレイション 変異型ルシフェラーゼ
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
GB0030727D0 (en) 2000-12-15 2001-01-31 Lumitech Uk Ltd Methods and kits for detecting kinase activity
GB0103622D0 (en) * 2001-02-14 2001-03-28 Univ Cambridge Tech Methods for detecting DNA polymerisation
CA2464942A1 (en) * 2001-11-14 2003-05-22 John Cavendish Day Signal system and elements used therein
US7413874B2 (en) 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
GB2400659B8 (en) 2003-04-17 2007-07-09 Cambrex Bio Science Nottingham Assay methods and materials
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP5188771B2 (ja) 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
JPWO2009066673A1 (ja) * 2007-11-20 2011-04-07 キッコーマン株式会社 核酸分析用組成物
CN103983634A (zh) * 2008-07-22 2014-08-13 普罗美加公司 基于adp检测的发光磷酸转移酶或atp水解酶测定
JP2011120559A (ja) 2008-12-26 2011-06-23 Kikkoman Corp ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
ES2543913T3 (es) 2010-11-02 2015-08-25 Promega Corporation Derivados de coelenterazina y métodos de uso de los mismos
EP3498709B1 (en) 2010-11-02 2025-12-31 Promega Corporation Luciferases from Oplophorus: Novel Coelenterazine Substrates and Their Methods of Use
EP4306649A3 (en) 2013-02-22 2024-04-03 Promega Corporation Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
WO2015116867A1 (en) 2014-01-29 2015-08-06 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6811173B2 (ja) 2014-09-11 2021-01-13 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
JP6510041B2 (ja) 2014-10-08 2019-05-08 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生物発光サクシネート検出アッセイ
DE102015215569A1 (de) 2015-08-14 2017-02-16 Robert Bosch Gmbh Verfahren zur Generierung eines Geheimnisses zwischen Teilnehmern eines Netzwerkes sowie dazu eingerichtete Teilnehmer des Netzwerks
JPWO2021162123A1 (enExample) 2020-02-14 2021-08-19
WO2024019165A1 (ja) 2022-07-22 2024-01-25 キッコーマン株式会社 微生物若しくは細胞又は微生物関連物質若しくは細胞関連物質を検出する方法及びキット
EP4656723A1 (en) 2023-01-27 2025-12-03 Kikkoman Corporation Luciferase variant
CN119931968B (zh) * 2024-05-10 2025-11-18 中国人民解放军军事科学院军事医学研究院 一种荧光素酶突变体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219737A (en) * 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
DE4123672A1 (de) 1991-07-17 1993-01-21 Basf Ag Siegelfaehige, peelbare kunststoffolie
ES2301160T3 (es) * 1994-01-03 2008-06-16 Promega Corporation Luciferasas mutantes.
GB9501170D0 (en) * 1994-03-23 1995-03-08 Secr Defence Luciferases

Also Published As

Publication number Publication date
EP0804587B1 (en) 2004-08-18
CA2210354A1 (en) 1996-07-25
NO322134B1 (no) 2006-08-21
CA2210354C (en) 2008-01-08
JP4342608B2 (ja) 2009-10-14
DK0804587T3 (da) 2004-10-11
IN186115B (enExample) 2001-06-23
JPH10512750A (ja) 1998-12-08
NO973349D0 (no) 1997-07-18
DE69633171D1 (de) 2004-09-23
AU4397396A (en) 1996-08-07
US6171808B1 (en) 2001-01-09
EP0804587A1 (en) 1997-11-05
NO973349L (no) 1997-09-19
DE69633171T2 (de) 2005-08-18
WO1996022376A1 (en) 1996-07-25

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