AU6562098A - Sequence alterations using homologous recombination - Google Patents

Sequence alterations using homologous recombination

Info

Publication number
AU6562098A
AU6562098A AU65620/98A AU6562098A AU6562098A AU 6562098 A AU6562098 A AU 6562098A AU 65620/98 A AU65620/98 A AU 65620/98A AU 6562098 A AU6562098 A AU 6562098A AU 6562098 A AU6562098 A AU 6562098A
Authority
AU
Australia
Prior art keywords
dna
sequence
targeting
recombinase
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU65620/98A
Other languages
English (en)
Inventor
Sushma Pati
David A Zarling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SRI International Inc
Original Assignee
SRI International Inc
Stanford Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SRI International Inc, Stanford Research Institute filed Critical SRI International Inc
Publication of AU6562098A publication Critical patent/AU6562098A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Contacts (AREA)
  • Superconductors And Manufacturing Methods Therefor (AREA)
  • Use Of Switch Circuits For Exchanges And Methods Of Control Of Multiplex Exchanges (AREA)
AU65620/98A 1997-03-21 1998-03-16 Sequence alterations using homologous recombination Abandoned AU6562098A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US4117397P 1997-03-21 1997-03-21
US60041173 1997-03-21
US08910367 1997-08-13
US08/910,367 US5948653A (en) 1997-03-21 1997-08-13 Sequence alterations using homologous recombination
PCT/US1998/005223 WO1998042727A1 (en) 1997-03-21 1998-03-16 Sequence alterations using homologous recombination

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2002300590A Division AU2002300590A1 (en) 1997-03-21 2002-08-09 Sequence alterations using homologous recombination

Publications (1)

Publication Number Publication Date
AU6562098A true AU6562098A (en) 1998-10-20

Family

ID=26717883

Family Applications (1)

Application Number Title Priority Date Filing Date
AU65620/98A Abandoned AU6562098A (en) 1997-03-21 1998-03-16 Sequence alterations using homologous recombination

Country Status (6)

Country Link
US (3) US5948653A (enExample)
EP (1) EP0977771A4 (enExample)
JP (1) JP2001518803A (enExample)
AU (1) AU6562098A (enExample)
CA (1) CA2284280A1 (enExample)
WO (1) WO1998042727A1 (enExample)

Families Citing this family (159)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040019916A1 (en) * 1992-04-24 2004-01-29 Zarling David A. In vivo homologous sequence targeting in eukaryotic cells
US20020108136A1 (en) * 1997-03-21 2002-08-08 Sri Transgenic animals produced by homologous sequence targeting
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
AU743305C (en) 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
EP1025217B1 (en) * 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
EP1038017A2 (en) * 1997-12-11 2000-09-27 Pangene Corporation The use of consensus sequences for targeted homologous gene isolation and recombination in gene families
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
US6004804A (en) * 1998-05-12 1999-12-21 Kimeragen, Inc. Non-chimeric mutational vectors
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
EP1105472A2 (en) * 1998-08-12 2001-06-13 Pangene Corporation Domain specific gene evolution
IL137868A0 (en) 1999-01-19 2001-10-31 Maxygen Inc Oligonucleotide mediated nucleic acid recombination
IL144657A0 (en) 1999-02-11 2002-06-30 Maxygen Inc High throughput mass spectrometry
WO2000056872A2 (en) * 1999-03-22 2000-09-28 Pangene Corporation High-throughput gene cloning and phenotypic screening
US6703240B1 (en) 1999-04-13 2004-03-09 Maxygar, Inc. Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes
US6977295B2 (en) * 1999-04-21 2005-12-20 Invitrogen Corporation Locked nucleic acid hybrids and methods of use
WO2001000819A1 (en) * 1999-06-25 2001-01-04 Chauhan, Sarita Targeted gene replacements in enteric bacteria using linear dna
US20020152494A1 (en) * 1999-09-14 2002-10-17 Sargent Roy Geoffrey Production of recombinant organisms
AU1456101A (en) 1999-11-03 2001-05-14 Maxygen, Inc. Antibody diversity generation
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
US7074983B2 (en) 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
AU1777301A (en) * 1999-11-19 2001-05-30 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
US7414170B2 (en) * 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
US6686180B2 (en) * 2000-04-04 2004-02-03 Baxter International Inc. Method for the production of bacterial toxins
US7045314B2 (en) * 2000-04-04 2006-05-16 Baxter International Inc. Method for the production of bacterial toxins
US20020061530A1 (en) * 2000-07-31 2002-05-23 Belotserkovskii Boris P. Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002018538A2 (en) * 2000-08-28 2002-03-07 Bionox Inc PROTOCOLS FOR THE GENERATION OF HIGH YIELD, SUPER-PRODUCTIVE TRANSGENIC PLANTS DISTURBED IN Ran/Ran-BINDING PROTEINS-MEDIATED CELLULAR PROCESS
AU2001296471A1 (en) * 2000-09-28 2002-04-08 Pangene Corporation High-throughput gene cloning and phenotypic screening
US20030096401A1 (en) * 2000-11-28 2003-05-22 Huse William D. Eukaryotic expression libraries and methods of use
JP4616988B2 (ja) * 2000-12-20 2011-01-19 株式会社アイシン・コスモス研究所 三本鎖ｄｎａ形成技術を応用したｄｎａ多型検出方法
JP4551058B2 (ja) * 2001-01-10 2010-09-22 ロンザ・ケルン・アーゲー モジュラートランスフェクション系
AU2002258603A1 (en) * 2001-03-23 2002-10-15 University Of Michigan Method of inhibiting cancerous cell proliferation using ras mutants of gdp-bound conformation
WO2002079495A2 (en) * 2001-03-27 2002-10-10 University Of Delaware Genomics applications for modified oligonucleotides
ES2305289T3 (es) 2001-04-20 2008-11-01 The Penn State Research Foundation Metodo de manipulacion de acidos nucleicos.
WO2002097059A2 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosome-based platforms
WO2003010333A2 (en) * 2001-07-24 2003-02-06 Affinium Pharmaceuticals Inc. Methods for gene disruption and uses thereof
CA2455013A1 (en) * 2001-07-27 2003-02-13 Francesca Storici Systems for in vivo site-directed mutagenesis using oligonucleotides
AU2002324941A1 (en) * 2001-09-07 2003-03-24 The Regents Of The University Of California Method of generating a transgenic livestock animal
US20030207327A1 (en) * 2001-09-27 2003-11-06 Kmiec Eric B. Coisogenic eukaryotic cell collections
US7468244B2 (en) * 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
US20030082551A1 (en) * 2001-09-28 2003-05-01 Zarling David A. High-throughput gene cloning and phenotypic screening
DK1438400T3 (da) 2001-10-01 2009-10-05 Dyax Corp Flerkædede eukaryote display-vektorer og anvendelser deraf
ES2564570T3 (es) 2002-03-01 2016-03-23 Codexis Mayflower Holdings, Llc Métodos, sistemas y software para la identificación de biomoléculas funcionales
EP1488335A4 (en) 2002-03-09 2006-11-15 Maxygen Inc OPTIMIZING CROSSOVER POINTS FOR THE ADJUSTED EVOLUTION
EP1501943A4 (en) * 2002-04-16 2005-06-22 Promega Corp METHOD FOR IMPROVING APPROVED RECOMBINATION
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
BRPI0309391B1 (pt) 2002-04-19 2018-10-30 Dsm Food Specialties B V vetor compreendendo ácido nucléico que codifica polipeptídeo com atividade de fosfolipase, célula transformada, preparado de proteína, método de preparação e métodos relacionados
WO2003104405A2 (en) * 2002-06-05 2003-12-18 The Regents Of The University Of California Methods for promoting homologous recombination
US20050233993A1 (en) * 2002-06-05 2005-10-20 Kadonaga James T Methods for promoting homologous recombination
EP2298904B1 (en) 2002-06-14 2017-05-24 BP Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
CA2489595C (en) * 2002-06-14 2013-05-28 Simon E. Hufton Recombination of nucleic acid library members
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
MXPA05001287A (es) 2002-08-06 2005-08-03 Verdia Llc Variantes de amino oxidasa ap1.
JP2006505291A (ja) * 2002-11-08 2006-02-16 ヘマテック，エルエルシー プリオンタンパク質活性が低減されたトランスジェニック有蹄動物及びその用途
PT2444102E (pt) * 2003-01-31 2015-09-17 Sinai School Medicine Terapia combinada para o tratamento de distúrbios de deficiência proteica
US7446098B2 (en) * 2003-02-18 2008-11-04 Mount Sinai School Of Medicine Of New York University Combination therapy for treating protein deficiencies
PL3023498T3 (pl) 2003-03-06 2019-05-31 Basf Enzymes Llc Amylazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i zastosowania
AU2004277368B2 (en) 2003-03-07 2011-01-20 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and mehods for making and using them
WO2004090099A2 (en) 2003-04-04 2004-10-21 Diversa Corporation Pectate lyases, nucleic acids encoding them and methods for making and using them
CA2521284C (en) 2003-04-29 2014-07-08 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
EP1484410B1 (en) * 2003-06-05 2011-11-02 Ajinomoto Co., Inc. Fermentation methods using modified bacteria with increased byproduct uptake.
DK1641910T3 (da) 2003-07-02 2013-05-21 Verenium Corp Glucanaser, nucleinsyrer, som koder for disse, og fremgangsmåder til at fremstille og benytte disse
EP2484687A3 (en) 2003-08-08 2012-11-14 Life Technologies Corporation Methods and compositions for seamless cloning of nucleic acid molecules
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
JP2007512838A (ja) 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法
WO2005062812A2 (en) * 2003-12-22 2005-07-14 The Johns Hopkins University A rAAV-BASED SYSTEM FOR SOMATIC CELL GENE DISRUPTION
WO2005104835A2 (en) 2004-04-22 2005-11-10 Kirin Beer Kabushiki Kaisha Transgenic animals and uses thereof
CA2735315A1 (en) 2004-06-09 2005-12-29 Pioneer Hi-Bred International, Inc. Plastid transit peptides
CA2570872A1 (en) 2004-06-16 2006-01-26 Diversa Corporation Compositions and methods for enzymatic decolorization of chlorophyll
EP1614755A1 (en) * 2004-07-07 2006-01-11 ARTEMIS Pharmaceuticals GmbH Target transgenesis of short hairpin RNA expression cassette using recombinase mediated cassette exchange
US20090038023A1 (en) 2005-03-10 2009-02-05 Verenium Corporation Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them
KR101393679B1 (ko) 2005-03-15 2014-05-21 비피 코포레이션 노쓰 아메리카 인코포레이티드 셀룰라제, 이것을 암호화하는 핵산 및 이들을 제조 및사용하는 방법
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
EP2932982B1 (en) 2005-05-17 2018-10-03 Amicus Therapeutics, Inc. A method for the treatment of pompe disease using 1-deoxynojirimycin and derivatives
US20100119525A1 (en) * 2005-08-01 2010-05-13 Mount Sinai Schoool Of Medicine Of New York University Method for extending longevity using npc1l1 antagonists
US20090324574A1 (en) 2006-02-02 2009-12-31 Verenium Corporation Esterases and Related Nucleic Acids and Methods
AU2006338208B2 (en) 2006-02-10 2013-10-24 Bp Corporation North America Inc. Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
EP2548954A1 (en) 2006-02-14 2013-01-23 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
KR101744157B1 (ko) 2006-03-07 2017-06-08 바스프 엔자임스 엘엘씨 알돌라제, 이것을 코딩하는 핵산과 그 제조 방법 및 사용 방법
CN101437954B (zh) 2006-03-07 2015-09-09 嘉吉公司 醛缩酶和编码醛缩酶的核酸以及它们的制备和使用方法
DK2069389T3 (en) 2006-08-04 2015-01-12 Bp Corp North America Inc Glucanases, nucleic acids encoding them, and processes for their preparation and use
ITMO20060269A1 (it) * 2006-09-06 2008-03-07 Sacmi Cooperativa Meccanici Imola Soc Coop Metodi di stampaggio a compressione
EP2617815B1 (en) 2006-09-21 2015-11-18 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
ES2451266T3 (es) 2006-09-21 2014-03-26 Dsm Ip Assets B.V. Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas
ES2531167T5 (es) 2006-12-21 2018-05-21 Basf Enzymes Llc Amilasas y glucoamilasas, ácidos nucleicos que las codifican y métodos para formarlas y utilizarlas
EP2074136A4 (en) 2007-01-30 2012-11-07 Verenium Corp ENZYMES FOR TREATING LIGNOCELLULOSE, FOR SUCH CODING NUCLEIC ACIDS, AND METHOD FOR THE PRODUCTION AND USE THEREOF
MX2010003600A (es) 2007-10-03 2010-12-14 Verenium Corp Xilanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
DK2238261T3 (da) 2008-01-03 2014-02-10 Verenium Corp Isomeraser, nukleinsyrer der koder for dem og fremgangsmåder til fremstilling og anvendelse deraf
DK2238242T5 (en) 2008-01-03 2015-08-03 Basf Enzymes Llc -Transferases and oxidoreductases, nucleic acids encoding FOR THEM, AND PROCESSES FOR THE STILL LING AND USE THEREOF
HUE026543T2 (hu) 2008-02-12 2016-06-28 Amicus Therapeutics Inc Eljárás betegségek gyógyászati chaperonnal történõ kezelésére adott válasz elõrejelzésére
EP2297310A2 (en) 2008-05-23 2011-03-23 E. I. du Pont de Nemours and Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
EP3502256A3 (en) 2008-09-26 2019-09-25 Tocagen Inc. Recombinant vectors
WO2010056746A1 (en) * 2008-11-11 2010-05-20 Amicus Therapeutics, Inc. Therapy regimens, dosing regimens and stable medicaments for the treatment of pompe disease
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
WO2010135588A2 (en) 2009-05-21 2010-11-25 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
EP2462230B1 (en) * 2009-08-03 2015-07-15 Recombinetics, Inc. Methods and compositions for targeted gene modification
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
BR112013003224A2 (pt) 2010-08-13 2016-06-07 Pioneer Hi Bred Int "construto de promotor quimérico, cassete de expressão, vetor de expressão, método de obtenção de uma planta, método para regular a expressão de um polinucleotídeo de interesse, polinucleotídeo, método para expressar um polinucleotídeo de interesse"
CN103370425B (zh) 2010-12-17 2019-03-19 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
EP2678434B1 (en) 2011-02-25 2018-11-07 Recombinetics, Inc. Genetically modified animals and methods for making the same
BR112014027468A2 (pt) 2012-05-04 2017-06-27 Du Pont polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.
AR091482A1 (es) 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
US10058078B2 (en) 2012-07-31 2018-08-28 Recombinetics, Inc. Production of FMDV-resistant livestock by allele substitution
EP3450549B1 (en) 2012-10-29 2020-09-30 University Of Rochester Artemisinin derivatives, methods for their preparation and their use as antimalarial agents
AU2014236154A1 (en) 2013-03-14 2015-09-17 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
AU2014236162A1 (en) 2013-03-14 2015-09-17 Arzeda Corp. Compositions having dicamba decarboxylase activity and methods of use
US10023877B2 (en) 2013-03-15 2018-07-17 Pioneer Hi-Bred International, Inc. PHI-4 polypeptides and methods for their use
CN106232820A (zh) 2013-08-16 2016-12-14 先锋国际良种公司 杀昆虫蛋白及其使用方法
US10667524B2 (en) 2013-09-13 2020-06-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3060682B1 (en) 2013-10-25 2023-08-30 Livestock Improvement Corporation Limited Genetic markers and uses therefor
WO2015100277A2 (en) 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
CN114763376A (zh) 2014-02-07 2022-07-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
PH12016501534B1 (en) 2014-02-07 2024-06-05 Du Pont Insecticidal proteins and methods for their use
UA126192C2 (uk) 2014-10-16 2022-08-31 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний білок та спосіб його застосування
CN114075267B (zh) 2015-01-15 2025-03-18 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN107529763B (zh) 2015-03-11 2021-08-20 先锋国际良种公司 Pip-72的杀昆虫组合及使用方法
JP6851319B2 (ja) * 2015-04-27 2021-03-31 ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア ヒト疾患のＣＲＩＳＰＲ／Ｃａｓ９媒介性の修正のためのデュアルＡＡＶベクター系
NZ738184A (en) 2015-05-18 2019-09-27 Univ California Methods and compositions for treating aging-associated impairments
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
US11198709B2 (en) 2015-08-06 2021-12-14 E. I. Du Pont De Nemours And Company Plant derived insecticidal proteins and methods for their use
EP3390431A1 (en) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3451837B1 (en) 2016-05-04 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX387506B (es) 2016-07-01 2025-03-18 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
WO2018111551A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11213028B2 (en) 2016-12-22 2022-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20190390219A1 (en) 2017-02-08 2019-12-26 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
WO2018208882A1 (en) 2017-05-11 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11634400B2 (en) 2017-08-19 2023-04-25 University Of Rochester Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents
EP3764798B1 (en) 2018-03-14 2025-12-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US11820791B2 (en) 2018-03-14 2023-11-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
AU2020343680A1 (en) 2019-09-06 2022-03-24 Amicus Therapeutics, Inc. Method for capturing and purification of biologics
WO2021142393A1 (en) 2020-01-10 2021-07-15 The Regents Of The University Of California Biosynthetic platform for the production of olivetolic avid and analogues of olivetolic acid
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
US20240043494A1 (en) 2020-08-07 2024-02-08 Amicus Therapeutics, Inc. Vesicle Targeting Proteins And Uses Of Same
EP4363437A1 (en) 2021-07-01 2024-05-08 Amicus Therapeutics, Inc. Neurotensin variants and tagged proteins comprising neurotensin or sortilin propeptide
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) * 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4888274A (en) * 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
US4950599A (en) * 1987-01-29 1990-08-21 Wolf Bertling Method for exchanging homologous DNA sequences in a cell using polyoma encapsulated DNA fragments
EP0437576B1 (en) * 1989-07-25 2002-07-03 Cell Genesys, Inc. Homologous recombination for universal donor cells and chimeric mammalian hosts
US5501967A (en) * 1989-07-26 1996-03-26 Mogen International, N.V./Rijksuniversiteit Te Leiden Process for the site-directed integration of DNA into the genome of plants
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
EP0945515A3 (en) * 1989-11-06 2002-08-21 Cell Genesys, Inc. Production of proteins using homologous recombination
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US5264618A (en) * 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
CA2116215C (en) * 1990-05-07 1998-12-22 Tapestry Pharmaceuticals, Inc. Diagnostic applications of double d-loop formation
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
WO1991019796A1 (en) * 1990-06-12 1991-12-26 Baylor College Of Medicine Method for homologous recombination in animal and plant cells
US5614396A (en) * 1990-06-14 1997-03-25 Baylor College Of Medicine Methods for the genetic modification of endogenous genes in animal cells by homologous recombination
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
US5612205A (en) * 1990-08-29 1997-03-18 Genpharm International, Incorporated Homologous recombination in mammalian cells
CA2095624C (en) * 1990-11-09 2000-06-13 Rafael D. Camerini-Otero Method of targeting dna
US5510473A (en) * 1990-11-09 1996-04-23 The United States Of American As Represented By The Secretary Of Health And Human Services Cloning of the recA gene from thermus aquaticus YT-1
WO1992015694A1 (en) * 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
WO1992020808A1 (en) 1991-05-15 1992-11-26 Cell Genesys, Inc. Genomic modifications with homologous dna targeting
WO1993003736A1 (en) * 1991-08-21 1993-03-04 Microprobe Corporation Cross-linking oligonucleotides for enzyme-mediated triple strand formation
US5506098A (en) * 1991-09-04 1996-04-09 Daikin Industries, Ltd. In situ hybridization method
WO1993007266A1 (en) * 1991-10-07 1993-04-15 Idaho Research Foundation, Inc. Genetic construct for selection of homologous recombinants on a single selective medium
EP0626012B1 (en) * 1992-02-11 2003-07-09 Cell Genesys, Inc. Homogenotization of gene-targeting events
US5459072A (en) * 1992-02-25 1995-10-17 Regents Of The University Of Minnesota Food-grade integration vectors for industrial bacterial strains
DE69333955D1 (de) * 1992-04-24 2006-02-02 Stanford Res Inst Int Targeting homologer sequenzen in eukaryotenzellen
WO1994004032A1 (en) * 1992-08-21 1994-03-03 The Regents Of The University Of California Composition and method for altering dna sequences by homologous recombination
US5468629A (en) * 1993-04-13 1995-11-21 Calhoun; Cornelia Method of promoting in vitro homologous recombination transfection in mammalian cells using the RecA protein
US5571688A (en) * 1993-05-06 1996-11-05 Mekalanos; John J. Method of detecting gene expression
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
CA2178729A1 (en) * 1993-12-09 1995-06-15 Eric B. Kmiec Compounds and methods for site-directed mutations in eukaryotic cells
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
JPH08140685A (ja) * 1994-11-22 1996-06-04 Takara Shuzo Co Ltd 部位特異的変異導入方法
EP0842283A1 (en) * 1995-07-21 1998-05-20 THE UNITED STATES GOVERNMENT as represented by THE DEPARTMENT OF HEALTH AND HUMAN SERVICES RecA-ASSISTED CLONING OF DNA
EP0842289B1 (en) * 1995-07-26 2004-10-20 Mixis France S.A. Homologous recombination in mismatch repair inactivated eukaryotic cells
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination

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