AU598490B2 - Human mutant tPA with modified glycosylation sites - Google Patents
Human mutant tPA with modified glycosylation sites Download PDFInfo
- Publication number
- AU598490B2 AU598490B2 AU65200/86A AU6520086A AU598490B2 AU 598490 B2 AU598490 B2 AU 598490B2 AU 65200/86 A AU65200/86 A AU 65200/86A AU 6520086 A AU6520086 A AU 6520086A AU 598490 B2 AU598490 B2 AU 598490B2
- Authority
- AU
- Australia
- Prior art keywords
- asn
- ser
- ala
- thr
- pro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 230000013595 glycosylation Effects 0.000 title abstract description 37
- 238000006206 glycosylation reaction Methods 0.000 title abstract description 37
- 108050006955 Tissue-type plasminogen activator Proteins 0.000 claims abstract description 286
- 102100033571 Tissue-type plasminogen activator Human genes 0.000 claims abstract description 253
- 101000801481 Homo sapiens Tissue-type plasminogen activator Proteins 0.000 claims abstract description 15
- 102000047823 human PLAT Human genes 0.000 claims abstract description 15
- 108020004414 DNA Proteins 0.000 claims description 153
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 103
- 108090000623 proteins and genes Proteins 0.000 claims description 96
- 239000013598 vector Substances 0.000 claims description 83
- 210000004027 cell Anatomy 0.000 claims description 73
- 150000001413 amino acids Chemical class 0.000 claims description 70
- 238000000034 method Methods 0.000 claims description 66
- 239000013612 plasmid Substances 0.000 claims description 58
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 51
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 42
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 42
- 108020004705 Codon Proteins 0.000 claims description 37
- 239000000203 mixture Substances 0.000 claims description 33
- 230000000694 effects Effects 0.000 claims description 32
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 27
- 230000035772 mutation Effects 0.000 claims description 27
- 210000005253 yeast cell Anatomy 0.000 claims description 27
- 238000002360 preparation method Methods 0.000 claims description 23
- 108091026890 Coding region Proteins 0.000 claims description 22
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 19
- 230000008569 process Effects 0.000 claims description 17
- 239000003795 chemical substances by application Substances 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 201000001441 melanoma Diseases 0.000 claims description 14
- 238000003556 assay Methods 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 12
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 11
- 229910052757 nitrogen Inorganic materials 0.000 claims description 11
- 125000003729 nucleotide group Chemical group 0.000 claims description 11
- 210000000349 chromosome Anatomy 0.000 claims description 10
- 210000004962 mammalian cell Anatomy 0.000 claims description 9
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 claims description 9
- 125000000539 amino acid group Chemical group 0.000 claims description 7
- 230000002759 chromosomal effect Effects 0.000 claims description 6
- 230000001131 transforming effect Effects 0.000 claims description 5
- 125000001433 C-terminal amino-acid group Chemical group 0.000 claims description 4
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000003937 drug carrier Substances 0.000 claims description 4
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims description 4
- FTCGGKNCJZOPNB-WHFBIAKZSA-N Asn-Gly-Ser Chemical compound NC(=O)C[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O FTCGGKNCJZOPNB-WHFBIAKZSA-N 0.000 claims description 3
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 150000007513 acids Chemical class 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 238000002741 site-directed mutagenesis Methods 0.000 claims description 3
- 230000001225 therapeutic effect Effects 0.000 claims description 3
- 238000001262 western blot Methods 0.000 claims description 3
- KLKHFFMNGWULBN-VKHMYHEASA-N Asn-Gly Chemical compound NC(=O)C[C@H](N)C(=O)NCC(O)=O KLKHFFMNGWULBN-VKHMYHEASA-N 0.000 claims description 2
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- LTFSLKWFMWZEBD-IMJSIDKUSA-N Ser-Asn Chemical compound OC[C@H](N)C(=O)N[C@H](C(O)=O)CC(N)=O LTFSLKWFMWZEBD-IMJSIDKUSA-N 0.000 claims description 2
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- 230000002194 synthesizing effect Effects 0.000 claims description 2
- OWQXAJQZLWHPBH-FXQIFTODSA-N Pro-Ser-Asn Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(O)=O OWQXAJQZLWHPBH-FXQIFTODSA-N 0.000 claims 5
- ZJBUILVYSXQNSW-YTWAJWBKSA-N Arg-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N)O ZJBUILVYSXQNSW-YTWAJWBKSA-N 0.000 claims 3
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- BRFJMRSRMOMIMU-WHFBIAKZSA-N Gly-Ala-Asn Chemical compound NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(O)=O BRFJMRSRMOMIMU-WHFBIAKZSA-N 0.000 claims 3
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- YRTOMUMWSTUQAX-FXQIFTODSA-N Asn-Pro-Asp Chemical compound NC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(O)=O YRTOMUMWSTUQAX-FXQIFTODSA-N 0.000 claims 2
- BUXAPSQPMALTOY-WHFBIAKZSA-N Cys-Glu Chemical compound SC[C@H](N)C(=O)N[C@H](C(O)=O)CCC(O)=O BUXAPSQPMALTOY-WHFBIAKZSA-N 0.000 claims 2
- KGVHCTWYMPWEGN-FSPLSTOPSA-N Gly-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)CN KGVHCTWYMPWEGN-FSPLSTOPSA-N 0.000 claims 2
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- OTXBNHIUIHNGAO-UWVGGRQHSA-N Leu-Lys Chemical compound CC(C)C[C@H](N)C(=O)N[C@H](C(O)=O)CCCCN OTXBNHIUIHNGAO-UWVGGRQHSA-N 0.000 claims 2
- OBXVZEAMXFSGPU-FXQIFTODSA-N Ser-Asn-Arg Chemical compound C(C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CO)N)CN=C(N)N OBXVZEAMXFSGPU-FXQIFTODSA-N 0.000 claims 2
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- UQFYNFTYDHUIMI-WHFBIAKZSA-N Ser-Gly-Ala Chemical compound OC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](N)CO UQFYNFTYDHUIMI-WHFBIAKZSA-N 0.000 claims 2
- 108010034529 leucyl-lysine Proteins 0.000 claims 2
- 239000008194 pharmaceutical composition Substances 0.000 claims 2
- CCUAQNUWXLYFRA-IMJSIDKUSA-N Ala-Asn Chemical compound C[C@H]([NH3+])C(=O)N[C@H](C([O-])=O)CC(N)=O CCUAQNUWXLYFRA-IMJSIDKUSA-N 0.000 claims 1
- XCVRVWZTXPCYJT-BIIVOSGPSA-N Ala-Asn-Pro Chemical compound C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N1CCC[C@@H]1C(=O)O)N XCVRVWZTXPCYJT-BIIVOSGPSA-N 0.000 claims 1
- BLIMFWGRQKRCGT-YUMQZZPRSA-N Ala-Gly-Lys Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCCN BLIMFWGRQKRCGT-YUMQZZPRSA-N 0.000 claims 1
- HJGZVLLLBJLXFC-LSJOCFKGSA-N Ala-His-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](C(C)C)C(O)=O HJGZVLLLBJLXFC-LSJOCFKGSA-N 0.000 claims 1
- ZSOICJZJSRWNHX-ACZMJKKPSA-N Ala-Ile Chemical compound CC[C@H](C)[C@@H](C([O-])=O)NC(=O)[C@H](C)[NH3+] ZSOICJZJSRWNHX-ACZMJKKPSA-N 0.000 claims 1
- OQWQTGBOFPJOIF-DLOVCJGASA-N Ala-Lys-His Chemical compound C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N OQWQTGBOFPJOIF-DLOVCJGASA-N 0.000 claims 1
- CHFFHQUVXHEGBY-GARJFASQSA-N Ala-Lys-Pro Chemical compound C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@@H]1C(=O)O)N CHFFHQUVXHEGBY-GARJFASQSA-N 0.000 claims 1
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- XNSKSTRGQIPTSE-ACZMJKKPSA-N Arg-Thr Chemical compound C[C@@H]([C@@H](C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N)O XNSKSTRGQIPTSE-ACZMJKKPSA-N 0.000 claims 1
- RJUHZPRQRQLCFL-IMJSIDKUSA-N Asn-Asn Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(O)=O RJUHZPRQRQLCFL-IMJSIDKUSA-N 0.000 claims 1
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- DATSKXOXPUAOLK-KKUMJFAQSA-N Asn-Tyr-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(O)=O DATSKXOXPUAOLK-KKUMJFAQSA-N 0.000 claims 1
- VHQOCWWKXIOAQI-WDSKDSINSA-N Asp-Gln-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(O)=O VHQOCWWKXIOAQI-WDSKDSINSA-N 0.000 claims 1
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-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Diabetes (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB858528321A GB8528321D0 (en) | 1985-11-18 | 1985-11-18 | Modified fibrinolytic agents |
| GB8528321 | 1985-11-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU6520086A AU6520086A (en) | 1987-05-21 |
| AU598490B2 true AU598490B2 (en) | 1990-06-28 |
Family
ID=10588363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU65200/86A Expired AU598490B2 (en) | 1985-11-18 | 1986-11-17 | Human mutant tPA with modified glycosylation sites |
Country Status (17)
| Country | Link |
|---|---|
| EP (1) | EP0225286B1 (forum.php) |
| JP (1) | JPS62272976A (forum.php) |
| KR (1) | KR920007666B1 (forum.php) |
| AT (1) | ATE78514T1 (forum.php) |
| AU (1) | AU598490B2 (forum.php) |
| DE (1) | DE3686136T2 (forum.php) |
| DK (1) | DK174977B1 (forum.php) |
| ES (1) | ES2043607T3 (forum.php) |
| FI (1) | FI91160C (forum.php) |
| GB (1) | GB8528321D0 (forum.php) |
| GR (1) | GR3006041T3 (forum.php) |
| IE (1) | IE57866B1 (forum.php) |
| IL (1) | IL80659A (forum.php) |
| NO (1) | NO175266C (forum.php) |
| NZ (1) | NZ218305A (forum.php) |
| PT (1) | PT83752B (forum.php) |
| ZA (1) | ZA868689B (forum.php) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU626323B2 (en) * | 1988-09-02 | 1992-07-30 | Genentech Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK175327B1 (da) * | 1984-10-01 | 2004-08-23 | Integrated Genetics Inc | Replicerbar ekspressionsvektor og rekombinant DNA-molekyle, som indkoder aktiv human livmodervævsplasminogen-aktivator, pattedyrscelle transformeret med vektoren og fremgangsmåde til fremstilling af aktiv human vævsplasminogen-aktivator (TPA) |
| ZA869626B (en) * | 1985-12-23 | 1987-10-28 | Chiron Corp | Peptide plasminogen activators |
| US5258298A (en) * | 1986-01-31 | 1993-11-02 | Genetics Institute, Inc. | Deletion and glycosylation mutant of human tissue plasminogen activator |
| US5002887A (en) * | 1986-01-31 | 1991-03-26 | Genetics Institute, Inc. | Truncated thrombolytic proteins |
| US5837518A (en) * | 1986-01-31 | 1998-11-17 | Genetics Institute, Inc. | Thrombolytic proteins |
| IE81073B1 (en) * | 1986-03-18 | 2000-01-12 | Genentech Inc | Modified human tissue-type plasminogen activator and its preparation |
| US5589361A (en) * | 1986-03-18 | 1996-12-31 | Genentech, Inc. | Human tissue-type plasminogen activator variant |
| US5047241A (en) * | 1986-07-11 | 1991-09-10 | American Home Products Corporation | Tris-Kringle plasminogen activator with novel K2 insert |
| EP0276846A3 (en) * | 1987-01-29 | 1989-07-26 | Zymogenetics, Inc. | Colony-stimulating factor derivatives |
| JPH084501B2 (ja) * | 1987-03-20 | 1996-01-24 | エーザイ株式会社 | 糖鎖に関する変異ｔＰＡ |
| SE8702562L (sv) * | 1987-06-18 | 1988-12-19 | Kabigen Ab | Nya fibrinolytiska enzymer |
| US6682733B1 (en) * | 1987-06-18 | 2004-01-27 | Roche Diagnostics, Gmbh | Fibrinolytic enzymes |
| US5266474A (en) * | 1987-06-24 | 1993-11-30 | Genentech, Inc. | Balanced inducible transcription system |
| US5302390A (en) * | 1987-07-01 | 1994-04-12 | Beecham Group Plc | Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment |
| WO1989000191A1 (en) * | 1987-07-06 | 1989-01-12 | Genetics Institute, Inc. | Novel thrombolytic proteins |
| AU1893988A (en) * | 1987-07-13 | 1989-01-19 | Collaborative Research Inc. | Nonglycosylated plasminogen activator and method of preparation |
| US5270198A (en) * | 1988-05-20 | 1993-12-14 | Genentech, Inc. | DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells |
| IL90146A (en) * | 1988-05-20 | 1994-10-07 | Genentech Inc | Glycosylation derivatives of tissue plasminogen activator |
| US5346824A (en) * | 1988-05-20 | 1994-09-13 | Genentech, Inc. | DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof |
| US5258180A (en) * | 1988-09-02 | 1993-11-02 | Genetech, Inc. | Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment |
| US5714145A (en) * | 1988-09-02 | 1998-02-03 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
| US5262170A (en) * | 1988-09-02 | 1993-11-16 | Genentech, Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells |
| WO1991015235A1 (en) * | 1990-03-30 | 1991-10-17 | Survival Technology, Inc. | t-PA ENHANCER PROFILE |
| US5242688A (en) * | 1990-12-24 | 1993-09-07 | Eli Lilly And Company | Method of treating thromboembolic disorders by administration of diglycosylated t-pa variants |
| PT786257E (pt) * | 1992-06-03 | 2003-12-31 | Genentech Inc | Variantes de glicosilacao do activador de plasminogenio tissular com propriedades terapeuticas meloradas |
| EP0663009A1 (en) * | 1993-08-04 | 1995-07-19 | Novartis AG | High molecular weight desulphatohirudin |
| US6706504B1 (en) | 1996-11-12 | 2004-03-16 | The Scripps Research Institute | Tissue type plasminogen activator (t-PA) variants: compositions and methods of use |
| EP2861614B1 (en) * | 2012-06-14 | 2016-11-30 | Portola Pharmaceuticals, Inc. | METHOD FOR PURIFICATION OF RECOMBINANT FACTOR Xa DERIVATIVES |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU563031B2 (en) * | 1982-05-05 | 1987-06-25 | Genentech Inc. | Human tissue plasminogen activator |
| AU572108B2 (en) * | 1983-01-19 | 1988-05-05 | Genentech Inc. | Human tpa production using vectors coding for dhfr protein |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX197183A (es) * | 1982-05-05 | 1994-02-28 | Genentech Inc | Activador de plasminogeno de tejido humano |
| WO1984001786A1 (en) * | 1982-10-28 | 1984-05-10 | Beecham Group Plc | Enzyme derivatives and their use in the treatment of thrombosis |
| DK175327B1 (da) * | 1984-10-01 | 2004-08-23 | Integrated Genetics Inc | Replicerbar ekspressionsvektor og rekombinant DNA-molekyle, som indkoder aktiv human livmodervævsplasminogen-aktivator, pattedyrscelle transformeret med vektoren og fremgangsmåde til fremstilling af aktiv human vævsplasminogen-aktivator (TPA) |
| DE3537176C2 (de) * | 1984-10-18 | 1994-06-09 | Zymogenetics Inc | Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung |
| JPH0811066B2 (ja) * | 1985-09-30 | 1996-02-07 | インテグレーテッド・ジエネティックス・インコーポレーテッド | 組換えｄｎａが生産するヒト子宮プラスミノ−ゲンアクチベ−タ− |
-
1985
- 1985-11-18 GB GB858528321A patent/GB8528321D0/en active Pending
-
1986
- 1986-11-13 ES ES86810518T patent/ES2043607T3/es not_active Expired - Lifetime
- 1986-11-13 AT AT86810518T patent/ATE78514T1/de not_active IP Right Cessation
- 1986-11-13 DE DE8686810518T patent/DE3686136T2/de not_active Expired - Lifetime
- 1986-11-13 EP EP86810518A patent/EP0225286B1/en not_active Expired - Lifetime
- 1986-11-14 FI FI864654A patent/FI91160C/fi not_active IP Right Cessation
- 1986-11-17 NO NO864569A patent/NO175266C/no not_active IP Right Cessation
- 1986-11-17 AU AU65200/86A patent/AU598490B2/en not_active Expired
- 1986-11-17 NZ NZ218305A patent/NZ218305A/en unknown
- 1986-11-17 ZA ZA868689A patent/ZA868689B/xx unknown
- 1986-11-17 PT PT83752A patent/PT83752B/pt not_active IP Right Cessation
- 1986-11-17 IE IE3028/86A patent/IE57866B1/en not_active IP Right Cessation
- 1986-11-17 JP JP61272033A patent/JPS62272976A/ja active Pending
- 1986-11-17 IL IL80659A patent/IL80659A/xx not_active IP Right Cessation
- 1986-11-17 DK DK198605491A patent/DK174977B1/da not_active IP Right Cessation
- 1986-11-18 KR KR1019860009732A patent/KR920007666B1/ko not_active Expired
-
1992
- 1992-10-22 GR GR920401405T patent/GR3006041T3/el unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU563031B2 (en) * | 1982-05-05 | 1987-06-25 | Genentech Inc. | Human tissue plasminogen activator |
| AU572108B2 (en) * | 1983-01-19 | 1988-05-05 | Genentech Inc. | Human tpa production using vectors coding for dhfr protein |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU626323B2 (en) * | 1988-09-02 | 1992-07-30 | Genentech Inc. | Tissue plasminogen activator having zymogenic or fibrin specific properties |
Also Published As
| Publication number | Publication date |
|---|---|
| DK549186A (da) | 1987-05-19 |
| KR920007666B1 (ko) | 1992-09-14 |
| ZA868689B (en) | 1987-06-24 |
| PT83752B (pt) | 1989-06-30 |
| DE3686136D1 (de) | 1992-08-27 |
| EP0225286B1 (en) | 1992-07-22 |
| AU6520086A (en) | 1987-05-21 |
| FI864654A0 (fi) | 1986-11-14 |
| DE3686136T2 (de) | 1992-12-17 |
| FI864654L (fi) | 1987-05-19 |
| FI91160C (fi) | 1994-05-25 |
| GB8528321D0 (en) | 1985-12-24 |
| IE863028L (en) | 1987-05-18 |
| JPS62272976A (ja) | 1987-11-27 |
| ES2043607T3 (es) | 1994-01-01 |
| IL80659A (en) | 1991-12-15 |
| EP0225286A3 (en) | 1987-10-07 |
| PT83752A (en) | 1986-12-01 |
| NO864569L (no) | 1987-05-19 |
| NO175266C (no) | 1994-09-28 |
| IL80659A0 (en) | 1987-02-27 |
| NO175266B (no) | 1994-06-13 |
| DK549186D0 (da) | 1986-11-17 |
| DK174977B1 (da) | 2004-04-05 |
| IE57866B1 (en) | 1993-05-05 |
| NZ218305A (en) | 1990-01-29 |
| KR870005094A (ko) | 1987-06-04 |
| EP0225286A2 (en) | 1987-06-10 |
| NO864569D0 (no) | 1986-11-17 |
| ATE78514T1 (de) | 1992-08-15 |
| FI91160B (fi) | 1994-02-15 |
| GR3006041T3 (forum.php) | 1993-06-21 |
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