AU2023207727A1 - Method of modulating vegf and uses thereof - Google Patents

Method of modulating vegf and uses thereof Download PDF

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Publication number
AU2023207727A1
AU2023207727A1 AU2023207727A AU2023207727A AU2023207727A1 AU 2023207727 A1 AU2023207727 A1 AU 2023207727A1 AU 2023207727 A AU2023207727 A AU 2023207727A AU 2023207727 A AU2023207727 A AU 2023207727A AU 2023207727 A1 AU2023207727 A1 AU 2023207727A1
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Australia
Prior art keywords
vegf
composition
gene
dcas9
dna
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Pending
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AU2023207727A
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English (en)
Inventor
Shaoshuai MAO
Wenbo PENG
Yidi SUN
Changyang ZHOU
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Epigenic Therapeutics Pte Ltd
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Epigenic Therapeutics Pte Ltd
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Publication of AU2023207727A1 publication Critical patent/AU2023207727A1/en
Assigned to EPIGENIC THERAPEUTICS PTE. LTD. reassignment EPIGENIC THERAPEUTICS PTE. LTD. Request for Assignment Assignors: EPIGENIC THERAPEUTICS, INC.
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
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    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01037—DNA (cytosine-5-)-methyltransferase (2.1.1.37)
    • C—CHEMISTRY; METALLURGY
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
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AU2023207727A 2022-01-14 2023-01-10 Method of modulating vegf and uses thereof Pending AU2023207727A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CNPCT/CN2022/071930 2022-01-14
CN2022071930 2022-01-14
PCT/CN2023/071521 WO2023134658A1 (en) 2022-01-14 2023-01-10 Method of modulating vegf and uses thereof

Publications (1)

Publication Number Publication Date
AU2023207727A1 true AU2023207727A1 (en) 2024-07-11

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ID=87280101

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AU2023207727A Pending AU2023207727A1 (en) 2022-01-14 2023-01-10 Method of modulating vegf and uses thereof

Country Status (9)

Country Link
US (1) US20250066772A1 (https=)
EP (1) EP4463551A1 (https=)
JP (1) JP2025502346A (https=)
KR (1) KR20240133745A (https=)
CN (1) CN118525093A (https=)
AU (1) AU2023207727A1 (https=)
CA (1) CA3247727A1 (https=)
TW (1) TW202342743A (https=)
WO (1) WO2023134658A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2025507990A (ja) * 2022-03-04 2025-03-21 エピジェニック・セラピューティクス・インコーポレイテッド ゲノム編集の組成物及び方法
WO2025261475A1 (zh) * 2024-06-21 2025-12-26 益杰立科(上海)生物科技有限公司 靶向血管内皮生长因子的工程化基因转录阻抑工具及用途

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7067317B2 (en) * 2000-12-07 2006-06-27 Sangamo Biosciences, Inc. Regulation of angiogenesis with zinc finger proteins
WO2021243105A1 (en) * 2020-05-28 2021-12-02 University Of Southern California Composition and method for treating retinal vascular disease with vegf gene disruption
CN111748583A (zh) * 2020-07-17 2020-10-09 池嘉栋 一种基于CRISPR/dCas9的诱导型DNA甲基化编辑系统

Also Published As

Publication number Publication date
EP4463551A1 (en) 2024-11-20
JP2025502346A (ja) 2025-01-24
CA3247727A1 (en) 2023-07-20
KR20240133745A (ko) 2024-09-04
CN118525093A (zh) 2024-08-20
WO2023134658A1 (en) 2023-07-20
TW202342743A (zh) 2023-11-01
US20250066772A1 (en) 2025-02-27

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