AU2022282057B2 - Novel dual helper plasmid - Google Patents

Novel dual helper plasmid Download PDF

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Publication number
AU2022282057B2
AU2022282057B2 AU2022282057A AU2022282057A AU2022282057B2 AU 2022282057 B2 AU2022282057 B2 AU 2022282057B2 AU 2022282057 A AU2022282057 A AU 2022282057A AU 2022282057 A AU2022282057 A AU 2022282057A AU 2022282057 B2 AU2022282057 B2 AU 2022282057B2
Authority
AU
Australia
Prior art keywords
gene
plasmid
aspects
rep
aav
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2022282057A
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English (en)
Other versions
AU2022282057A1 (en
AU2022282057A9 (en
Inventor
Joo Seok HAN
Yoon Hyung HWANG
Hoon Young Kong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Elisigen Inc
Original Assignee
Elisigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US17/750,241 external-priority patent/US12305188B2/en
Application filed by Elisigen Inc filed Critical Elisigen Inc
Publication of AU2022282057A1 publication Critical patent/AU2022282057A1/en
Publication of AU2022282057A9 publication Critical patent/AU2022282057A9/en
Application granted granted Critical
Publication of AU2022282057B2 publication Critical patent/AU2022282057B2/en
Assigned to Elisigen Inc. reassignment Elisigen Inc. Request to Amend Deed and Register Assignors: NEURACLE GENETICS INC.
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/50—Vectors for producing vectors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
AU2022282057A 2021-05-27 2022-05-27 Novel dual helper plasmid Active AU2022282057B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR20210068364 2021-05-27
KR10-2021-0068364 2021-05-27
US17/750,241 US12305188B2 (en) 2021-05-27 2022-05-20 Dual helper plasmid
US17/750,241 2022-05-20
PCT/KR2022/007553 WO2022250491A1 (en) 2021-05-27 2022-05-27 Novel dual helper plasmid

Publications (3)

Publication Number Publication Date
AU2022282057A1 AU2022282057A1 (en) 2023-12-21
AU2022282057A9 AU2022282057A9 (en) 2024-01-25
AU2022282057B2 true AU2022282057B2 (en) 2025-01-23

Family

ID=84228978

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2022282057A Active AU2022282057B2 (en) 2021-05-27 2022-05-27 Novel dual helper plasmid

Country Status (6)

Country Link
US (1) US20260092291A1 (https=)
EP (1) EP4347850A4 (https=)
JP (1) JP7854125B2 (https=)
KR (1) KR102685652B1 (https=)
AU (1) AU2022282057B2 (https=)
WO (1) WO2022250491A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024211780A1 (en) * 2023-04-07 2024-10-10 Regenxbio Inc. Compositions and methods for recombinant aav production
WO2025083617A1 (en) * 2023-10-18 2025-04-24 Nanocell Therapeutics Holdings B.V. Methods and compositions for nucleic construct delivery

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040033751A (ko) * 2002-10-15 2004-04-28 주식회사 삼양제넥스 재조합 아데노부속바이러스 유전자 전달체 생산용 벡터시스템

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5872005A (en) 1994-11-03 1999-02-16 Cell Genesys Inc. Packaging cell lines for adeno-associated viral vectors
JP2003501042A (ja) 1999-05-27 2003-01-14 ジェノボ， インコーポレイテッド 非哺乳動物ウイルス由来のキャリアベクターを使用する、組換えウイルスの産生のための組成物および方法
DK2368903T3 (da) * 2010-03-26 2014-05-05 Deutsches Krebsforsch Adenovirusafledt hjælpevirus til fremme af produktion af rekombinant parvovirus
US10550405B2 (en) 2017-03-15 2020-02-04 The University Of North Carolina At Chapel Hill Rational polyploid adeno-associated virus vectors and methods of making and using the same
EP3612635A2 (en) 2017-04-18 2020-02-26 GlaxoSmithKline Intellectual Property Development Limited Methods for adeno-associated viral vector production
GB201706090D0 (en) * 2017-04-18 2017-05-31 Glaxosmithkline Ip Dev Ltd Methods for adeno-associated viral vector production
GB201800903D0 (en) 2018-01-19 2018-03-07 Oxford Genetics Ltd Vectors
CA3112883A1 (en) * 2018-10-25 2020-04-30 Baxalta Incorporated Aav triple-plasmid system
DK3722434T3 (da) * 2019-04-12 2022-10-24 Freeline Therapeutics Ltd Plasmidsystem
WO2022097647A1 (ja) 2020-11-04 2022-05-12 株式会社シンプロジェン 統合型プラスミド

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040033751A (ko) * 2002-10-15 2004-04-28 주식회사 삼양제넥스 재조합 아데노부속바이러스 유전자 전달체 생산용 벡터시스템

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TANG Q. et al., 'Two-Plasmid Packaging System for Recombinant Adeno-Associated Virus', BioResearch Open Access. (2020), vol. 9, no. 1, pages 219-228. *

Also Published As

Publication number Publication date
EP4347850A1 (en) 2024-04-10
JP7854125B2 (ja) 2026-05-01
US20260092291A1 (en) 2026-04-02
AU2022282057A1 (en) 2023-12-21
JP2024519175A (ja) 2024-05-08
KR102685652B1 (ko) 2024-07-19
AU2022282057A9 (en) 2024-01-25
WO2022250491A1 (en) 2022-12-01
KR20230152768A (ko) 2023-11-03
EP4347850A4 (en) 2025-04-02

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SREP Specification republished
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Owner name: ELISIGEN INC.

Free format text: FORMER NAME(S): NEURACLE GENETICS INC.