AU2020214090B2 - Inhibition of unintended mutations in gene editing - Google Patents

Inhibition of unintended mutations in gene editing Download PDF

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Publication number
AU2020214090B2
AU2020214090B2 AU2020214090A AU2020214090A AU2020214090B2 AU 2020214090 B2 AU2020214090 B2 AU 2020214090B2 AU 2020214090 A AU2020214090 A AU 2020214090A AU 2020214090 A AU2020214090 A AU 2020214090A AU 2020214090 B2 AU2020214090 B2 AU 2020214090B2
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editing
fragment
protease
cytidine deaminase
seq
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AU2020214090A1 (en
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Jia Chen
Xingxu HUANG
Lijie Wang
Bei YANG
Li Yang
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ShanghaiTech University
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Shanghai Technology, University of
ShanghaiTech University
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04004—Adenosine deaminase (3.5.4.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
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    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21111—Aqualysin 1 (3.4.21.111)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22044—Nuclear-inclusion-a endopeptidase (3.4.22.44)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)

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  • Crystallography & Structural Chemistry (AREA)
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  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2020214090A 2019-02-02 2020-02-03 Inhibition of unintended mutations in gene editing Active AU2020214090B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2022287550A AU2022287550B2 (en) 2019-02-02 2022-12-12 Inhibition of unintended mutations in gene editing
AU2025256226A AU2025256226A1 (en) 2019-02-02 2025-10-24 Inhibition of unintended mutations in gene editing

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2019074577 2019-02-02
CNPCT/CN2019/074577 2019-02-02
PCT/CN2020/074218 WO2020156575A1 (en) 2019-02-02 2020-02-03 Inhibition of unintended mutations in gene editing

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AU2020214090A1 AU2020214090A1 (en) 2021-09-16
AU2020214090B2 true AU2020214090B2 (en) 2022-09-15

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US (3) US11384353B2 (https=)
EP (1) EP3918083A4 (https=)
JP (3) JP7518549B2 (https=)
KR (1) KR20210126042A (https=)
CN (4) CN116656676A (https=)
AU (3) AU2020214090B2 (https=)
BR (1) BR112021015092A2 (https=)
CA (1) CA3128283A1 (https=)
IL (1) IL285194A (https=)
SG (1) SG11202108303SA (https=)
WO (1) WO2020156575A1 (https=)
ZA (2) ZA202105895B (https=)

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CN116656676A (zh) 2023-08-29
CN113661248A (zh) 2021-11-16
WO2020156575A1 (en) 2020-08-06
JP2024109660A (ja) 2024-08-14
SG11202108303SA (en) 2021-08-30
AU2022287550B2 (en) 2025-07-24
AU2022287550A1 (en) 2023-02-02
ZA202212490B (en) 2024-09-25
US11384353B2 (en) 2022-07-12
JP7518549B2 (ja) 2024-07-18
AU2025256226A1 (en) 2025-11-13
JP7761294B2 (ja) 2025-10-28
AU2020214090A1 (en) 2021-09-16
US20230094769A1 (en) 2023-03-30
CN116355966A (zh) 2023-06-30
JP2026012727A (ja) 2026-01-27
KR20210126042A (ko) 2021-10-19
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ZA202105895B (en) 2024-07-31
BR112021015092A2 (pt) 2022-10-11
IL285194A (en) 2021-09-30
US20220064626A1 (en) 2022-03-03
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