AU2018338781B2 - Click based ligation - Google Patents

Click based ligation Download PDF

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Publication number
AU2018338781B2
AU2018338781B2 AU2018338781A AU2018338781A AU2018338781B2 AU 2018338781 B2 AU2018338781 B2 AU 2018338781B2 AU 2018338781 A AU2018338781 A AU 2018338781A AU 2018338781 A AU2018338781 A AU 2018338781A AU 2018338781 B2 AU2018338781 B2 AU 2018338781B2
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AU
Australia
Prior art keywords
click
reaction
catalyst
molecule
methyl
Prior art date
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Active
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AU2018338781A
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English (en)
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AU2018338781A1 (en
Inventor
Thomas FRISCHMUTH
Sascha SERDJUKOW
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baseclick GmbH
Original Assignee
Baseclick GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP17194093.5A external-priority patent/EP3461832A1/en
Application filed by Baseclick GmbH filed Critical Baseclick GmbH
Publication of AU2018338781A1 publication Critical patent/AU2018338781A1/en
Application granted granted Critical
Publication of AU2018338781B2 publication Critical patent/AU2018338781B2/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/109—Characterised by chemical treatment chemical ligation between nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2018338781A 2017-09-29 2018-09-28 Click based ligation Active AU2018338781B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP17194093.5 2017-09-29
EP17194093.5A EP3461832A1 (en) 2017-09-29 2017-09-29 Click based ligation
EP18164071 2018-03-26
EP18164071.5 2018-03-26
PCT/EP2018/076495 WO2019063803A1 (en) 2017-09-29 2018-09-28 LIGATURE BASED ON A CLICK REACTION

Publications (2)

Publication Number Publication Date
AU2018338781A1 AU2018338781A1 (en) 2020-02-06
AU2018338781B2 true AU2018338781B2 (en) 2023-04-20

Family

ID=63683912

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2018338781A Active AU2018338781B2 (en) 2017-09-29 2018-09-28 Click based ligation

Country Status (9)

Country Link
US (1) US20200361984A1 (enExample)
EP (1) EP3688004A1 (enExample)
JP (1) JP2020536524A (enExample)
KR (1) KR20200057708A (enExample)
CN (1) CN111183144A (enExample)
AU (1) AU2018338781B2 (enExample)
BR (1) BR112020004041A2 (enExample)
CA (1) CA3070514A1 (enExample)
WO (1) WO2019063803A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021130151A1 (en) 2019-12-23 2021-07-01 Baseclick Gmbh Method of amplifying mrnas and for preparing full length mrna libraries
EP3842532A1 (en) 2019-12-23 2021-06-30 baseclick GmbH Method of amplifying mrnas and for preparing full length mrna libraries
MX2022011504A (es) 2020-03-19 2022-10-07 Baseclick Gmbh Arnm modificados para desarrollo de vacunas.
EP4046651A1 (en) 2021-02-23 2022-08-24 baseclick GmbH Method for producing an mrna tumor vaccine
WO2023194331A1 (en) 2022-04-04 2023-10-12 Ecole Polytechnique Federale De Lausanne (Epfl) CONSTRUCTION OF SEQUENCING LIBRARIES FROM A RIBONUCLEIC ACID (RNA) USING TAILING AND LIGATION OF cDNA (TLC)
EP4585695A1 (en) * 2024-01-15 2025-07-16 Leica Microsystems CMS GmbH Label and marker for analysing a biological sample

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA973642B (en) * 1996-04-26 1997-11-25 Merck & Co Inc DNA vaccine formulations.
WO2003101972A1 (en) * 2002-05-30 2003-12-11 The Scripps Research Institute Copper-catalysed ligation of azides and acetylenes
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
GB0706243D0 (en) * 2007-03-30 2007-05-09 Univ Southampton Modified nucleic acids
EP2239053A1 (en) * 2009-04-09 2010-10-13 baseclick GmbH Click chemistry on heterogeneous catalysts
US8680260B2 (en) * 2009-05-15 2014-03-25 Riken 18F-labeled azide compound, reagent for 18F-labeling and method for 18F-labeling of alkyne compound using same
US20110210017A1 (en) * 2010-03-01 2011-09-01 Lai Rebecca Y Fabrication of electrochemical biosensors via click chemistry
US20130046083A1 (en) * 2011-08-16 2013-02-21 Tom Brown Oligonucleotide ligation
WO2014016202A1 (en) * 2012-07-22 2014-01-30 Universität Basel Methods for catalytic alkylation of nucleic acids
EP2940150A1 (en) * 2014-04-29 2015-11-04 Baseclick GmbH Self-assembly of DNA Origami: a new diganostic tool
WO2016196492A1 (en) * 2015-06-01 2016-12-08 Occidental College Methods, compositions, and kits using heterogeneous catalysts
US10155797B2 (en) * 2015-09-11 2018-12-18 The Governors Of The University Of Alberta Binding-induced DNA nanomachines

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Routh, A., et al. "ClickSeq: fragmentation-free next-generation sequencing via click ligation of adaptors to stochastically terminated 3′-azido cDNAs." Journal of Molecular Biology, 2015, 427(16), p: 2610-2616. *

Also Published As

Publication number Publication date
KR20200057708A (ko) 2020-05-26
CN111183144A (zh) 2020-05-19
EP3688004A1 (en) 2020-08-05
WO2019063803A1 (en) 2019-04-04
BR112020004041A2 (pt) 2020-09-08
JP2020536524A (ja) 2020-12-17
US20200361984A1 (en) 2020-11-19
CA3070514A1 (en) 2019-04-04
AU2018338781A1 (en) 2020-02-06

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