AU2018200532B2

AU2018200532B2 - Method for processing high-quality barley grass meal replacement powder rich in barley grass enzyme

Info

Publication number: AU2018200532B2
Application number: AU2018200532A
Authority: AU
Inventors: Yanjun Feng; Min Zhang
Original assignee: Jiangnan University
Current assignee: Jiangnan University
Priority date: 2017-02-21
Filing date: 2018-01-23
Publication date: 2019-01-31
Anticipated expiration: 2038-01-23
Also published as: CN106858345A; AU2018200532A1

Abstract

A method for processing high-quality barley grass meal replacement powder rich in barley grass enzyme belongs to the technical field of research and development of health foods. The present invention prepares a product by preprocessing barley grass, preparing 5 nano barley grass powder, preparing nano bean dreg powder, preparing barley grass enzyme, and preparing barley grass meal replacement powder. The barley grass meal replacement powder produced by using the method in the present invention can be quickly dissolved in 30 s after being brewed with warm water, has an instant property that is improved by 20% compared with like products, and has advantages such as a good 10 anti-adhesion property, high color stability, a good taste, and balanced nutrition. The product has an anti-oxidation capability, and can enhance the immunity and the weight loss effects after being taken for a long time, meeting the development trend of "green, environmentally friendly, and healthy" in the current food industry.

Description

METHOD FOR PROCESSING HIGH-QUALITY BARLEY GRASS MEAL REPLACEMENT POWDER RICH IN BARLEY GRASS ENZYME

BACKGROUND

Technical Field

The present invention belongs to the technical field of research and development of health foods, and relates to a method for processing a high-quality barley grass meal replacement powder rich in barley grass enzyme, and specifically, to a method for processing and producing an instant health food of barley grass meal replacement powder 10 by using nano barley grass powder, nano bean dreg powder, and barley grass enzyme powder that is dried through low-temperature ultrasonic auxiliary spray as main raw materials and then adding other nutriments and food additives (such as sodium hexametaphosphate, maltodextrin, and stevioside) as auxiliary materials for processing.

Related Art

With the rapid development of the social economy and the improvement of people's living standard, people increasingly seek for delicious diets but gradually neglect propemess of trophic structures of the diets, resulting in more phenomena of imbalance trophic structures of the bodies, such as obesity, astriction, three-highs (hypertension, hyperlipidemia, and hyperglycemia), cardiovascular diseases, and even cancers. Obesity 20 significantly affects the work and life. Long-term obesity may further increase risks of having cardiovascular diseases, diabetes, cancers, and a lot of other diseases. Optimizing the dietary structure to reasonably increase dietary fibers, intake necessary fatty acids, and balance nutrition is significantly important in preventing and improving symptoms of obesity and astriction, keeping healthy, and improving life quality.

Meal replacement, as its name suggests, is a type of foods that replace some or all of the main meals. Meal replacement powder is characterized in high fiber, relatively low calories, and strong satiety sensation, and achieves, following the ideas of balancing

2018200532 23 Jan 2018 nutrition and losing weight healthily, an objective of losing weight healthily by adjusting and balancing intake of various nutriments in bodies. The meal replacement powder is safe and effective to be eaten, and therefore is popular with people who want to lose weight. The meal replacement powder does not damage the body if being eaten reasonably, being a 5 truly green diet food. Meal replacement powder made from cereals and meal replacement powder made from fruits and vegetables are common in the market.

Barley grass is rich in various nutritive elements such as plant flavonoid, active ferment, chlorophyll, protein, amino acid, vitamin, and mineral substances, and has great development value. Barley grass powder made by drying barley grass contains rich 10 nutriments, such as plant flavonoid, active ferment, chlorophyll, protein, vitamin, and mineral substances, and is an extraordinary biological antioxidant, immunopotentiator, and cell nutritional agent. Enzyme, as a health food, refers to a microbial agent that is produced by fermenting various probiotics with plants such as fresh vegetables and fruits as raw materials, and that contains rich enzymes, vitamins, mineral substances, and secondary 15 metabolic products. Researches show that enzyme foods have functions of diminishing inflammation, antibiosis, antioxidation, blood purification, promoting cellular regeneration, and enhancing immunity. Some enzyme foods further have the antitumer function. Due to the good health care function, the enzyme foods are widely popular in Japan and Taiwan. Although some enzyme foods appear in the domestic market, the quantities are still very 20 small. Therefore, there is still a bright development prospect. Adding enzymes into the meal replacement powder can add nutritional ingredients of the meal replacement powder, so that the trophic structure thereof is more reasonable, contributing to the health of the people who want to lose weight.

Nano pulverization is a novel pulverization technology. A material pulverized by a 25 nano pulverizer may have a nano-scale particle size, and a product made from this material has a better solubility compared with the conventional pulverization technology. Low-temperature ultrasonic auxiliary spray drying is a novel spray drying technology that increases the surface area of a liquid by using the characteristic that ultrasonic waves may form ultrasonic spray at surfaces of a lot of thermosensitive materials. Within a material,

2018200532 23 Jan 2018 especially on an organization interface, most of the ultrasonic energy is converted into heat, resulting in localized high temperature and promoting moisture leakage, so as to improve the evaporation intensity and efficiency, and to reduce the evaporation temperature. Consequently, the low-temperature ultrasonic auxiliary spray drying has advantages such as a quick drying speed, a low temperature, a low final water content, and materials cannot be damaged or blown away relative to the conventional spray drying, can better protect active substances in the materials, thereby reserving the functions to the greatest extent.

Zhai Junna et al., (2011) take 30 women light physical laborers as experimental subjects, ask them to have nutritional meal replacements of soybean protein (which is mixed nutritional meal replacement powder that uses soybean protein as the main ingredient, where the main ingredients include soybean protein, fructose, cereal fiber, and pectin; and further have multiple types of vitamins and mineral substances, mainly including soybean phospholipid, potassium chloride, calcium caseinate, reduced iron, ascorbic acid, vitamin, niacin, vitamin, riboflavin, and the like. The edible method is mixing 25 g nutritional meal replacement powder into 300 mL warm boiled water and then eating for each meal, energy contained in each meal being 200 Kcal) for breakfasts and dinners; and ask them to have normal lunches, where the energy is between 700 Kcal to 800 Kcal, without any other snacks other than 1000 mL to 1500 mL water for every day. Six weeks later, various indexes such as weight and body girth of the experimental subjects are all reduced, where the indexes of weight and waistline are significantly different, p<0.0l; skinfold thicknesses at all parts are obviously reduced; the moisture percentage in the body is increased; the fat percentage and the body mass index are obviously reduced; the triglyceride and the low-density lipoprotein in the blood are reduced, and the high-density lipoprotein in the blood is increased; the basal metabolic rate and the step index are obviously increased; and the systolic pressure, the diastolic pressure, and the quartile are obviously reduced. These results indicate that in the process of losing weight, the nutritional meal replacement can not only improve the somatotypes of human bodies to significantly optimize the morphological indexes, but also can improve the physical quality, so that body composition indexes, blood indexes, indexes of body function level, and indexes of athletic ability level that represent the physical quality are all significantly 3

2018200532 23 Jan 2018 improved. Dai Yongmei et al., (2015) discussed the impacts of intervention of nutritional matching of low energy, low glycemic index, and high protein (two lows and one high) (energy supply ratios of protein, carbohydrate, and fat are 35%, 35%, and 30% respectively) on body mass and body compositions of women with simple obesity. 65 women with 5 simple obesity are divided into two groups. A test group includes 35 people, and uses an intervention solution of the nutritional matching of two lows and one high, the intervention time being 35 d. A control group includes 30 people, and uses a low-energy intervention solution of nutritional matching. The weight loss effects of the nutritional meals of two lows and one high are discussed by comparing changes in body mass and 10 body compositions of the two groups before and after the intervention. The results indicate that body mass, body mass indexes, body fat, body fat percentages, waist-to-hip ratios, and visceral fat areas of the two groups after the intervention are all reduced. Differences of the test group before and after the intervention are obviously greater than those of the control group, and the differences are statistically significant (p<0.05).

However, differences in skeletal muscles, body protein mass, intracellular water, and extracellular water of the two groups before and after the weight loss are not statistically significant (p>0.05). These results indicate that the intervention means of the nutritional matching of two lows and one high can effectively reduce the body mass, the body fat, and the visceral fat of women with simple obesity, but does not affect the mass, proteins, 20 and water contents of slim bodies, being a scientific and healthy nutritional intervention manner for weight loss. Barley grass meal replacement powder produced by using this method uses bean dreg powder that is also rich in soybean protein as a main raw material, and is added with barley grass powder that is rich in multiple types of vitamins and mineral substances, meeting the standard of the nutritional matching of two lows and one high. 25 Moreover, after being optimized by using a flavor agent, the barley grass meal replacement powder also has the functions of improving somatotypes and physical qualities of human bodies, and has a better taste, being more suitable for being eaten by people.

Zhao Hong et al., disclose a blueberry enzyme meal replacement powder and a preparation method therefor (Chinese Patent Application No. 201510948318.7). Fruits, 30 vegetables, and nuts are cleaned, then are performed with dicing and foreign matter

2018200532 23 Jan 2018 removing, are inserted into a sugar solution to be dehydrated, and then are taken out to be pulverized into 80 meshes after being frozen and dried for a period of time; and obtained mixed powder is physically mixed with amorphophallus konjac powder, and is added with one to five parts of blueberry enzyme powder to mix evenly. In this way, the blueberry enzyme meal replacement powder is obtained. The blueberry enzyme meal replacement powder obtained by using this method has balance nutrition and can eliminate toxins in bodies and have a cosmetic effect. However, because raw materials are all coarsely pulverized, the particle size of the obtained meal replacement powder is relatively large, and the solubility is relatively bad. In this method, the main raw materials of barley grass powder, bean dreg powder, and enzyme powder in the meal replacement powder are respectively performed with nano pulverization and low-temperature ultrasonic auxiliary spray drying. Therefore, the obtained meal replacement powder has tiny particles and a good solubility. Sodium hexametaphosphate is added, so that the dissolution of the meal replacement powder is promoted. Zinc chloride is added, facilitating to keep stability of chlorophyll in the barley grass enzyme.

Dong Dandan et al., disclose a meal replacement powder rich in dietary fibers and a preparation method therefor (Chinese Patent Application No. 201510756947.X). Meal replacement powder prepared by using this method is made from the following mass percentages of substances: 40%-90% of soluble dietary fiber; 5%-15% of resistant starch; 0.05%-1% of sweetening agents; 20%-40% of animal protein; 10%-30% of plant protein; and 0.001%-10% of decavitamin. In this method, the resistant starch, the plant protein, and the decavitamin are first pre-mixed progressively, and then other ingredients are added to mix evenly; water is added into the mixture to prepare a water suspension; and finally the water suspension is performed with spray drying, to select a finished product of a target particle size. Although the meal replacement powder obtained by using this method does not affect the absorptivity of a human body to the dietary fiber while supplementing nutrient substances required for the human body, difficulty level of the spray drying is undoubtedly increased by performing the spray drying after all materials are mixed together. As a result, the yield rate of the product is reduced, and the costs are greatly increased. This method uses the process of first respectively making all substances into powder and 5

2018200532 23 Jan 2018 then mixing the powder. The method is simple with relatively low costs, and uses the process of low-temperature ultrasonic auxiliary spray drying, so as to maintain functional ingredients in the enzyme to the greatest extent. Therefore, the functional loss of the product is relatively small.

Sun Jingbo discloses an ultramicro instant nutritional meal replacement powder of black fungus, blueberry, and red yeast rice, and a preparation method therefor (Chinese Patent Application No. 201610170736.2). The meal replacement powder is prepared according to the following parts by weight: 4.5-5.5 parts of black fungus ultrafine powder, 1.5-2.0 parts of blueberry fruit powder, 0.5-0.7 parts of red yeast powder, 0.3-0.4 parts of 10 defatted black bean powder, 0.2-0.3 parts of haw powder, 0.3-0.5 parts of chitosan, 0.04-0.06 parts of dried orange peel powder, 0.02-0.03 parts of lily flower powder, 0.0002-0.0003 parts of ethyl maltol, and 3.5-4.0 parts of sorbitol. This method also uses the process of making all substances into powder and then mixing the powder. However, the main ingredient black fungus is performed with superfine grinding, significantly 15 increasing solubility of the meal replacement powder. Yang Wei et al., disclose a fruit and vegetable enzyme nutritional meal replacement powder (Chinese Patent Application No. 201510357807.5) that is composed of components in the following percentages by weight: 20%-40% of amorphophallus konjac gum, 10%-30% of juice powder, 10%-30% of water-soluble dietary fibers, 30%-50% of isomaltooligosaccharide, l%-20% of composite 20 plant enzymes, and 0.1%-0.5% of sucralose. The fruit and vegetable enzyme nutritional meal replacement powder provided in the present invention fully reserves fragrance and nutrition of fruits, and may also bring in a strong satiety sensation to consumers, so as to achieve the effect of losing weight by reducing intake of foods. In addition, the composite plant enzymes are added, so that surplus fat stored in the body can be quickly and 25 effectively decomposed and burned to be promoted to be evacuated, so that the consumers achieve effects of weight loss and being slim. This method uses nano pulverization that has better effects than superfine grinding, and the obtained meal replacement powder has better solubility. In addition, self-made barley grass enzyme is added, enhancing the weight loss effects and achieving better effects.

2018200532 23 Jan 2018

SUMMARY

An objective of the present invention is to provide a method for processing high-quality barley grass meal replacement powder rich in barley grass enzyme. An enzyme is dried to obtain power and is added into meal replacement powder, thereby 5 enriching uses of an enzyme product, and enhancing the weight loss effects of the meal replacement powder. The process used in the method is simple, and the costs are low. An obtained product has a good instant property, a good anti-adhesion property, high color stability, a good taste, and balanced nutrition, has an anti-oxidation capability, and can enhance the immunity and the weight loss effects after being taken for a long time.

A method for processing high-quality barley grass meal replacement powder rich in barley grass enzyme, including the steps of preparation of nano barley grass powder, preparation of nano bean dreg powder, preparation of barley grass enzyme powder and compounding, wherein the steps are:

(1) preprocessing of barley grass: freezing and drying the barley grass after the barley grass is performed with foreign matter removing, cleaning, and dicing, wherein conditions for the freezing and drying are that the temperature of a heating board is 23-25°C, the drying time is 24 h, a vacuum degree is 129.8-130.2 Pa, a material thickness is 4.5-5.5 mm, prefreezing is performed for 4 h at -6°C, and the freezing temperature is -28 to -32°C;

(2) preparation of nano barley grass powder: grinding the barley grass that is frozen 20 and dried in step (1), passing the same through an 80-mesh sieve to obtain coarse barley grass powder, and then grinding the coarse barley grass powder to a nano scale in an impact mill by using a grinding ball, so as to prepare the nano barley grass powder, wherein a mass ratio of the grinding ball to the coarse barley grass powder is 4:1, the temperature is reduced to 0 to -5 °C in a cooling circulation system that uses 5% of aqueous glycerol 25 solution as a cooling liquid, an impact and vibration frequency is 150-200 Hz, the time is

8-10 h, and the coarse barley grass powder is pulverized to have an average particle size of 100-500 nm, so that the nano barley grass powder is prepared;

(3) preparation of nano bean dreg powder: initially grinding bean dregs and then

2018200532 23 Jan 2018 grinding coarse bean dreg powder to the nano scale in an impact mill, wherein a mass ratio of the grinding ball to the coarse bean dreg powder is 4:1, the temperature is reduced to 0 to -5 °C in a cooling circulation system that uses 5% of aqueous glycerol solution as the cooling liquid, an impact and vibration frequency is 150-200 Hz, the time is 8-10 h, and the coarse bean dreg powder is pulverized to have an average particle size of 100-500 nm, so that the nano bean dreg powder is prepared;

(4) preparation of barley grass enzyme powder: adding maltodextrin into barley grass enzyme, a ratio of an addition amount of the maltodextrin to a content of an enzyme solid matter being 1:1; and performing low-temperature ultrasonic auxiliary spray drying to 10 obtain barley grass enzyme powder, wherein the temperature of the auxiliary spray drying is 10-15°C, ultrasonic processing at 30-45 W is performed for 25-40 min, a feeding concentration when the auxiliary spray drying is performed is 6%-9%, an inlet temperature is 140±3°C, an outlet temperature is 90±3°C, and a rotational speed of an atomizer is 15000 r/min;

(5) preparation of barley grass meal replacement powder: evenly mixing the foregoing materials and other materials according to the following parts by mass, to obtain the barley grass meal replacement powder: 25-35 parts by mass of nano bean dreg powder, 25-35 parts by mass of refined amorphophallus konjac powder, 15-25 parts by mass of nano barley grass powder, 5-10 parts by mass of protein powder, 6-8 parts by mass of fish collagen, 0.5 parts by mass of sodium hexametaphosphate, 0.3 parts by mass of zinc chloride, 0.5-0.7 parts by mass of citric acid, 0.2-0.4 parts by mass of stevioside, and 0.2 parts by mass of flavoring essence.

The beneficial effects of the present invention are:

1. Compared with a production process of first mixing raw materials and then drying 25 the same, the process of first respectively making the raw materials into powder and then mixing the powder that is used in this method is simpler, is convenient for operation, has low costs, and is easy to be implemented and promoted.

2. The average particle size of the meal replacement powder is reduced by performing

2018200532 23 Jan 2018 nano pulverization to main raw materials of barley grass powder and bean dreg powder, so that the meal replacement powder has a better solubility, and is more suitable for being brewed to take by people.

3. Functional ingredients in the barley grass enzyme are reserved to the greatest extent by using a low-temperature ultrasonic auxiliary spray drying process, so that the functional loss of the barley grass enzyme is relatively small, and the weight loss effects of the meal replacement powder are enhanced to the greatest extent.

DETAILED DESCRIPTION

Embodiment 1: a method for processing a high-quality instant barley grass meal 10 replacement powder (in small batches)

Nano barley grass powder is prepared by freezing and drying barley grass after the barley grass is performed with foreign matter removing, cleaning, and dicing; grinding the frozen and dried barley grass, and passing the same through an 80-mesh sieve to obtain coarse barley grass powder; and then grinding the coarse barley grass powder to a nano 15 scale in an impact mill. After bean dregs are pulverized preliminarily, and coarse bean dreg powder is pulverized to the nano scale in the impact mill, so as to prepare nano bean dreg powder. An amount of maltodextrin is added into self-made barley grass enzyme, and low-temperature ultrasonic auxiliary spray drying is performed to obtain barley grass enzyme powder. The foregoing materials and other materials are evenly mixed according 20 to the following parts by mass, to obtain the barley grass meal replacement powder: 30 g bean dreg powder, 30 g refined amorphophallus konjac powder, 20 g barley grass powder, 10 g protein powder, 8 g fish collagen, 0.5 g sodium hexametaphosphate, 0.3 g zinc chloride, 0.7 g citric acid, 0.3 g stevioside, and 0.2 g flavoring essence.

The product obtained can be quickly dissolved in 30 s after being brewed with warm 25 water. After being tested, in three main indexes that represent the instant property, wettability and dispersibility of this product are respectively 21.6% and 95.3% higher than like products sold in the market, and solubility of this product is equivalent to that of the like products, representing a good instant property. After being assessed by sense organs,

2018200532 23 Jan 2018 this product has a relatively good taste, and has relatively strong satiety sensation after being taken. After being tested, a total phenol content in this product reaches 1.864 mg/g; SOD activity reaches 340.5 U/g; and a clearance rate of hydroxyl radicals may reach 52.8% and a clearance rate of DPPH radicals may reach 65.7%, being respectively 25.7% and 5 29.8% higher than the like products sold in the market. In this way, this product shows better anti-oxidation effects. After the product is stored for one week, changes in a color difference value δΕ are smaller than 5%, representing relatively good color stability. After an amount of the meal replacement powder is dissolved in water, the adhesion property (indicated by the mass of remaining adhesive blocks after being filtered) is 10 reduced by 18.7% compared with the like products sold in the market, representing a relatively good anti-adhesion property.

Embodiment 2: a method for processing a high-quality instant barley grass meal replacement powder (in medium batches)

Nano barley grass powder is prepared by freezing and drying barley grass after the barley grass is performed with foreign matter removing, cleaning, and dicing; grinding the frozen and dried barley grass, and passing the same through an 80-mesh sieve to obtain coarse barley grass powder; and then grinding the coarse barley grass powder to a nano scale in an impact mill. After bean dregs are pulverized preliminarily, and coarse bean dreg powder is pulverized to the nano scale in the impact mill, so as to prepare nano bean 20 dreg powder. An amount of maltodextrin is added into self-made barley grass enzyme, and low-temperature ultrasonic auxiliary spray drying is performed to obtain barley grass enzyme powder. The foregoing materials and other materials are evenly mixed according to the following parts by mass, to obtain the barley grass meal replacement powder: 300 g bean dreg powder, 300 g refined amorphophallus konjac powder, 200 g barley grass 25 powder, 100 g protein powder, 80 g fish collagen, 5.0 g sodium hexametaphosphate, 3.0 g zinc chloride, 7.0 g citric acid, 3.0 g stevioside, and 2.0 g flavoring essence.

The product obtained can be quickly dissolved in 30 s after being brewed with warm water. After being tested, in three main indexes that represent the instant property, wettability and dispersibility of this product are respectively 22.3% and 94.6% higher than

2018200532 23 Jan 2018 like products sold in the market, and solubility of this product is equivalent to that of the like products, representing a good instant property. After being assessed by sense organs, this product has a relatively good taste, and has relatively strong satiety sensation after being taken. After being tested, a total phenol content in this product reaches 1.864 mg/g;

SOD activity reaches 340.5 U/g; and a clearance rate of hydroxyl radicals may reach 50.9% and a clearance rate of DPPH radicals may reach 63.2%, being respectively 22.5% and 27.6% higher than the like products sold in the market. In this way, this product shows better anti-oxidation effects. After the product is stored for one week, changes in a color difference value δΕ are smaller than 5%, representing relatively good color stability.

After an amount of the meal replacement powder is dissolved in water, the adhesion property (indicated by the mass of remaining adhesive blocks after being filtered) is reduced by 18.7% compared with the like products sold in the market, representing a relatively good anti-adhesion property.

Embodiment 3: a method for processing a high-quality instant barley grass meal 15 replacement powder (in larger batches)

Nano barley grass powder is prepared by freezing and drying barley grass after the barley grass is performed with foreign matter removing, cleaning, and dicing; grinding the frozen and dried barley grass, and passing the same through an 80-mesh sieve to obtain coarse barley grass powder; and then grinding the coarse barley grass powder to a nano 20 scale in an impact mill. After bean dregs are pulverized preliminarily, and coarse bean dreg powder is pulverized to the nano scale in the impact mill, so as to prepare nano bean dreg powder. An amount of maltodextrin is added into self-made barley grass enzyme, and low-temperature ultrasonic auxiliary spray drying is performed to obtain barley grass enzyme powder. The foregoing materials and other materials are evenly mixed according 25 to the following parts by mass, to obtain the barley grass meal replacement powder: 3 kg bean dreg powder, 3 kg refined amorphophallus konjac powder, 2 kg barley grass powder, 1 kg protein powder, 0.8 kg fish collagen, 0.05 kg sodium hexametaphosphate, 0.03 kg zinc chloride, 0.07 kg citric acid, 0.03 kg stevioside, and 0.02 kg flavoring essence.

The product obtained can be quickly dissolved in 30 s after being brewed with warm

2018200532 23 Jan 2018 water. After being tested, in three main indexes that represent the instant property, wettability and dispersibility of this product are respectively 22.3% and 94.6% higher than like products sold in the market, and solubility of this product is equivalent to that of the like products, representing a good instant property. After being assessed by sense organs, 5 this product has a relatively good taste, and has relatively strong satiety sensation after being taken. After being tested, a total phenol content in this product reaches 1.972 mg/g;

SOD activity reaches 360.4 U/g; and a clearance rate of hydroxyl radicals may reach 53.51% and a clearance rate of DPPH radicals may reach 61.32%, being respectively 23.9% and 30.4% higher than the like products sold in the market. In this way, this 10 product shows better anti-oxidation effects. After the product is stored for one week, changes in a color difference value 5E are smaller than 5%, representing relatively good color stability. After an amount of the meal replacement powder is dissolved in water, the adhesion property (indicated by the mass of remaining adhesive blocks after being filtered) is reduced by 18.7% compared with the like products sold in the market, representing a 15 relatively good anti-adhesion property.

The reference to any prior art in this specification is not, and should not be taken as, an acknowledgement of any form of suggestion that such prior art forms part of the common general knowledge.

It will be understood that the term “comprise” and any of its derivatives (eg 20 comprises, comprising) as used in this specification is to be taken to be inclusive of features to which it refers, and is not meant to exclude the presence of any additional features unless otherwise stated or implied.

Claims

(1) preprocessing of barley grass: freezing and drying the barley grass after the barley

5 grass is performed with foreign matter removing, cleaning, and dicing, wherein conditions for the freezing and drying are that the temperature of a heating board is 23-25°C, the drying time is 24 h, a vacuum degree is 129.8-130.2 Pa, a material thickness is 4.5-5.5 mm, prefreezing is performed for 4 h at -6°C, and the freezing temperature is -28 to -32°C;

1. A method for processing high-quality barley grass meal replacement powder rich in barley grass enzyme, comprising the steps:
(2) preparation of nano barley grass powder: grinding the barley grass that is frozen 10 and dried in step (1), passing the same through an 80-mesh sieve to obtain coarse barley grass powder, and then grinding the coarse barley grass powder to a nano scale in an impact mill by using a grinding ball, so as to prepare the nano barley grass powder, wherein a mass ratio of the grinding ball to the coarse barley grass powder is 4:1, the temperature is reduced to 0 to -5 °C in a cooling circulation system that uses 5% of aqueous glycerol 15 solution as a cooling liquid, an impact and vibration frequency is 150-200 Hz, the time is

8-10 h, and the coarse barley grass powder is pulverized to have an average particle size of 100-500 nm, so that the nano barley grass powder is prepared;

(3) preparation of nano bean dreg powder: initially grinding bean dregs and then grinding coarse bean dreg powder to the nano scale in an impact mill, wherein a mass ratio

20 of the grinding ball to the coarse bean dreg powder is 4:1, the temperature is reduced to 0 to -5 °C in a cooling circulation system that uses 5% of aqueous glycerol solution as the cooling liquid, an impact and vibration frequency is 150-200 Hz, the time is 8-10 h, and the coarse bean dreg powder is pulverized to have an average particle size of 100-500 nm, so that the nano bean dreg powder is prepared;

25 (4) preparation of barley grass enzyme powder: adding maltodextrin into barley grass enzyme, a ratio of an addition amount of the maltodextrin to a content of an enzyme solid matter being 1:1; and performing low-temperature ultrasonic auxiliary spray drying to obtain barley grass enzyme powder, wherein the temperature of the auxiliary spray drying

2018200532 23 Jan 2018 is 10-15°C, ultrasonic processing at 30-45 W is performed for 25-40 min, a feeding concentration when the auxiliary spray drying is performed is 6%-9%, an inlet temperature is 140±3°C, an outlet temperature is 90±3°C, and a rotational speed of an atomizer is 15000 r/min;

5 the barley grass enzyme being formed by fermenting a saccharomycete for 12 h at the temperature of 32 °C with an inoculum size of 0.67% and with 5.3% of sucrose added; and (5) preparation of barley grass meal replacement powder: evenly mixing the foregoing materials and other materials according to the following parts by mass, to obtain the barley grass meal replacement powder: 25-35 parts by mass of nano bean dreg powder, 25-35 10 parts by mass of refined amorphophallus konjac powder, 15-25 parts by mass of nano barley grass powder, 5-10 parts by mass of protein powder, 6-8 parts by mass of fish collagen, 0.5 parts by mass of sodium hexametaphosphate, 0.3 parts by mass of zinc chloride, 0.5-0.7 parts by mass of citric acid, 0.2-0.4 parts by mass of stevioside, and 0.2 parts by mass of flavoring essence.

15 2. The method for processing high-quality barley grass meal replacement powder rich in barley grass enzyme according to claim 1, wherein the barley grass is barley grass has a length of 5-15 cm.
3. The method for processing high-quality barley grass meal replacement powder rich in barley grass enzyme according to claim 1, wherein the barley grass enzyme in step (4) is 20 formed by fermenting a saccharomycete, and key indexes thereof comprise that a content of a solid matter is 31%, a content of chlorophyll is 2.72 mg/mL, and SOD activity is 295 U/mL.