AU2017391167B2 - Non-human animals having an engineered immunoglobulin lambda light chain locus - Google Patents

Non-human animals having an engineered immunoglobulin lambda light chain locus Download PDF

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Publication number
AU2017391167B2
AU2017391167B2 AU2017391167A AU2017391167A AU2017391167B2 AU 2017391167 B2 AU2017391167 B2 AU 2017391167B2 AU 2017391167 A AU2017391167 A AU 2017391167A AU 2017391167 A AU2017391167 A AU 2017391167A AU 2017391167 B2 AU2017391167 B2 AU 2017391167B2
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Australia
Prior art keywords
human
rodent
light chain
gene segments
gene
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AU2017391167A
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AU2017391167A1 (en
Inventor
Chunguang GUO
Faith HARRIS
Natasha Levenkova
Lynn Macdonald
John Mcwhirter
Andrew J. Murphy
Naxin Tu
Vera VORONINA
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AU2017391167A 2016-11-04 2017-11-03 Non-human animals having an engineered immunoglobulin lambda light chain locus Active AU2017391167B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2024200582A AU2024200582A1 (en) 2016-11-04 2024-01-31 Non-human animals having an engineered immunoglobulin lambda light chain locus

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662417845P 2016-11-04 2016-11-04
US62/417,845 2016-11-04
US201762567932P 2017-10-04 2017-10-04
US62/567,932 2017-10-04
PCT/US2017/060006 WO2018128691A1 (en) 2016-11-04 2017-11-03 Non-human animals having an engineered immunoglobulin lambda light chain locus

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2024200582A Division AU2024200582A1 (en) 2016-11-04 2024-01-31 Non-human animals having an engineered immunoglobulin lambda light chain locus

Publications (2)

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AU2017391167A1 AU2017391167A1 (en) 2019-05-30
AU2017391167B2 true AU2017391167B2 (en) 2024-02-15

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AU2017391167A Active AU2017391167B2 (en) 2016-11-04 2017-11-03 Non-human animals having an engineered immunoglobulin lambda light chain locus
AU2024200582A Pending AU2024200582A1 (en) 2016-11-04 2024-01-31 Non-human animals having an engineered immunoglobulin lambda light chain locus

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Country Status (26)

Country Link
US (2) US10820582B2 (OSRAM)
EP (4) EP3407709B9 (OSRAM)
JP (5) JP6884860B2 (OSRAM)
KR (4) KR20240055863A (OSRAM)
CN (2) CN109996441B (OSRAM)
AU (2) AU2017391167B2 (OSRAM)
BR (1) BR112019008675A2 (OSRAM)
CA (1) CA3038720A1 (OSRAM)
CY (2) CY1123491T1 (OSRAM)
DK (3) DK3766343T3 (OSRAM)
ES (3) ES2915609T3 (OSRAM)
FI (1) FI4082334T3 (OSRAM)
HR (3) HRP20250695T1 (OSRAM)
HU (3) HUE061980T2 (OSRAM)
IL (2) IL308197A (OSRAM)
LT (3) LT4082334T (OSRAM)
MA (2) MA53935A (OSRAM)
MX (2) MX385857B (OSRAM)
PL (3) PL3766343T3 (OSRAM)
PT (3) PT3766343T (OSRAM)
RS (3) RS66884B1 (OSRAM)
RU (2) RU2757665C2 (OSRAM)
SG (2) SG11201903344XA (OSRAM)
SI (3) SI3407709T1 (OSRAM)
SM (3) SMT202000558T1 (OSRAM)
WO (1) WO2018128691A1 (OSRAM)

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DK3766343T3 (da) 2016-11-04 2022-06-20 Regeneron Pharma Ikke-humane dyr med et manipuleret immunoglobulin-lambda-letkædelocus
LT3772927T (lt) 2018-03-24 2025-02-10 Regeneron Pharmaceuticals, Inc. Genetiškai modifikuotos pelės arba žiurkės, skirtos gaminti terapinius antikūnus prieš peptidų- mhc kompleksus, jų gamybos būdai ir panaudojimas
CN116420679B (zh) 2018-03-26 2025-10-03 瑞泽恩制药公司 用于测试治疗剂的人源化啮齿动物
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LT3407709T (lt) 2020-10-12
US20180125043A1 (en) 2018-05-10
IL266282B1 (en) 2023-12-01
SG11201903344XA (en) 2019-05-30
EP4567119A3 (en) 2025-09-24
DK3766343T3 (da) 2022-06-20
MA44091A (fr) 2018-10-24
JP2021090455A (ja) 2021-06-17
SI3407709T1 (sl) 2020-10-30
PL3407709T3 (pl) 2021-01-11
WO2018128691A1 (en) 2018-07-12
MX2019005256A (es) 2019-08-05
PT3766343T (pt) 2022-05-30
EP3766343A1 (en) 2021-01-20
HRP20250695T1 (hr) 2025-08-01
KR102658529B1 (ko) 2024-04-19
RU2019112589A (ru) 2020-12-04
RU2757665C2 (ru) 2021-10-20
ES2823305T3 (es) 2021-05-06
SMT202000558T1 (it) 2020-11-10
CN109996441A (zh) 2019-07-09
IL266282A (en) 2019-06-30
JP2023053400A (ja) 2023-04-12
EP3407709B9 (en) 2021-10-06
US12433264B2 (en) 2025-10-07
DK3407709T3 (da) 2020-08-31
IL308197A (en) 2024-01-01
NZ753456A (en) 2021-08-27
IL266282B2 (en) 2024-04-01
RS66884B1 (sr) 2025-07-31
SI4082334T1 (sl) 2025-06-30
LT3766343T (lt) 2022-06-10
ES2915609T3 (es) 2022-06-23
CY1123491T1 (el) 2022-03-24
SMT202500204T1 (it) 2025-07-22
PT3407709T (pt) 2020-08-20
HRP20201403T1 (hr) 2020-11-27
HUE052087T2 (hu) 2021-04-28
KR20240055863A (ko) 2024-04-29
EP4082334B1 (en) 2025-03-26
BR112019008675A2 (pt) 2019-07-09
EP3407709B1 (en) 2020-07-29
CY1125474T1 (el) 2025-05-09
EP3407709A1 (en) 2018-12-05
KR102492433B1 (ko) 2023-01-30
HUE061980T2 (hu) 2023-09-28
PT4082334T (pt) 2025-05-21
FI4082334T3 (fi) 2025-06-10
CN114369157A (zh) 2022-04-19
RS63390B1 (sr) 2022-08-31
KR20210134059A (ko) 2021-11-08
SI3766343T1 (sl) 2022-06-30
JP7386292B2 (ja) 2023-11-24
MA53935A (fr) 2021-12-22
CN109996441B (zh) 2022-02-08
JP6884860B2 (ja) 2021-06-09
KR102319069B1 (ko) 2021-11-01
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