AU2017286096B2 - Cell culture device and methods - Google Patents

Cell culture device and methods Download PDF

Info

Publication number
AU2017286096B2
AU2017286096B2 AU2017286096A AU2017286096A AU2017286096B2 AU 2017286096 B2 AU2017286096 B2 AU 2017286096B2 AU 2017286096 A AU2017286096 A AU 2017286096A AU 2017286096 A AU2017286096 A AU 2017286096A AU 2017286096 B2 AU2017286096 B2 AU 2017286096B2
Authority
AU
Australia
Prior art keywords
gel
microfluidic
cells
organoid
microfluidic channel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2017286096A
Other languages
English (en)
Other versions
AU2017286096A1 (en
Inventor
Arnaud Nicolas
Sebastiaan Johannes TRIETSCH
Paul Vulto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mimetas BV
Original Assignee
Mimetas BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NL2016965A external-priority patent/NL2016965B1/en
Application filed by Mimetas BV filed Critical Mimetas BV
Publication of AU2017286096A1 publication Critical patent/AU2017286096A1/en
Application granted granted Critical
Publication of AU2017286096B2 publication Critical patent/AU2017286096B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/28—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Vascular Medicine (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2017286096A 2016-06-15 2017-06-12 Cell culture device and methods Active AU2017286096B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
NL2016965 2016-06-15
NL2016965A NL2016965B1 (en) 2016-06-15 2016-06-15 Cell culture device.
NL2018033 2016-12-21
NL2018033 2016-12-21
PCT/EP2017/064302 WO2017216113A2 (en) 2016-06-15 2017-06-12 Cell culture device and methods

Publications (2)

Publication Number Publication Date
AU2017286096A1 AU2017286096A1 (en) 2019-01-17
AU2017286096B2 true AU2017286096B2 (en) 2022-01-20

Family

ID=59215734

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017286096A Active AU2017286096B2 (en) 2016-06-15 2017-06-12 Cell culture device and methods

Country Status (9)

Country Link
US (1) US11629319B2 (enExample)
EP (1) EP3472301B1 (enExample)
JP (1) JP6967535B2 (enExample)
CN (1) CN109804057B (enExample)
AU (1) AU2017286096B2 (enExample)
BR (1) BR112018075920A2 (enExample)
CA (1) CA3027616A1 (enExample)
RU (1) RU2741806C2 (enExample)
WO (1) WO2017216113A2 (enExample)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11760965B2 (en) 2017-10-19 2023-09-19 Georgia Tech Research Corporation Mesofluidic device for culture of cell aggregates
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
CN108486035B (zh) * 2018-03-26 2021-11-26 西北大学 一种三维类器官的液滴培养方法
NL2022085B1 (en) * 2018-11-28 2020-06-25 Mimetas B V Device for assessing mechanical strain induced in or by cells
KR102209346B1 (ko) * 2019-03-13 2021-01-29 재단법인대구경북과학기술원 미세관통막 기반의 수화젤 마이크로웰 어레이 및 이를 이용한 3차원 단일세포 분리 및 배양방법
US12024696B2 (en) * 2019-05-08 2024-07-02 Molecular Devices (Austria) GmbH System and method for organoid culture
US20220238042A1 (en) * 2019-05-10 2022-07-28 Oregon Health & Science University Engineered bone marrow model
AU2020283390A1 (en) * 2019-05-28 2021-12-23 Duke University Methods and apparatuses for patient-derived micro-organospheres
KR102233992B1 (ko) * 2019-06-28 2021-03-29 건양대학교 산학협력단 면역화학진단 세포 칩 구조체
WO2021040874A1 (en) * 2019-08-28 2021-03-04 Massachusetts Institute Of Technology In vitro tissue plate
CN110564674B (zh) * 2019-09-29 2021-05-11 广东工业大学 一种干细胞铺底工作液及其应用
CN114585746A (zh) * 2019-10-17 2022-06-03 公立大学法人横滨市立大学 药物毒性评价方法
NL2024202B1 (en) * 2019-11-08 2021-07-20 Mimetas B V Microfluidic cell culture system
WO2021113924A1 (en) * 2019-12-12 2021-06-17 The Walter And Eliza Hall Institute Of Medical Research Organoid cultures
EP3907007A1 (de) 2020-05-08 2021-11-10 Technische Universität Wien Mikrofluidische vorrichtung
US20210394177A1 (en) * 2020-06-19 2021-12-23 The Texas A&M University System Systems and methods for mimicking a blood vessel of a patient
NL2026038B1 (en) * 2020-07-09 2022-03-15 Mimetas B V Microfluidic cell culture device
CN112143642B (zh) * 2020-08-28 2022-06-14 上海交通大学 用于体外培养的血管化肿瘤微流控器官芯片及其制备方法
CA3173455A1 (en) 2020-09-04 2022-03-10 9493662 Canada Inc. Multi-well device, kits and methods for analysis of cells
EP3998332A4 (en) * 2020-09-30 2022-09-28 Beijing Daxiang Biotech Co., Ltd 3D MULTIORGAN COCULTURE CHIP
JP7565008B2 (ja) * 2020-10-07 2024-10-10 ウシオ電機株式会社 マイクロ流体デバイス及びマイクロ流体デバイスの使用方法
WO2022087382A1 (en) * 2020-10-22 2022-04-28 The Board Of Regents Of The University Of Texas System High throughput cell migration assay plates and methods of fabrication
US20220127553A1 (en) * 2020-10-22 2022-04-28 Molecular Devices (Austria) GmbH Microplates for automating organoid cultivation
CN112358966B (zh) * 2020-10-23 2022-03-22 上海交通大学 一种滑动式微流控器官芯片及其制备方法
EP4255633A1 (en) * 2020-12-01 2023-10-11 Agency for Science, Technology and Research A device and method for vascularising a cell aggregate
US20240076593A1 (en) * 2021-02-19 2024-03-07 Molecular Devices (Austria) GmbH Methods for organoid passaging using microplate well units
KR20230156765A (ko) * 2021-03-16 2023-11-14 에이전시 포 사이언스, 테크놀로지 앤드 리서치 3d 세포 배양 및 인 비트로 조직 모델을 위한 웰 인서트 및 장치
JP2024513007A (ja) * 2021-04-09 2024-03-21 セルアートジェン インコーポレイテッド 細胞集合体の培養のための微細流体の懸滴培養デバイス
US12480079B2 (en) 2021-04-23 2025-11-25 Massachusetts Institute Of Technology Fluidic platforms for perfusable vascularized tissues with infiltrates
CN113755328A (zh) * 2021-09-08 2021-12-07 中山大学 一种类器官培养装置及类器官培养方法
EP4423234A1 (en) 2021-10-29 2024-09-04 Universität Bern Microfluidic cell culturing device
USD1028280S1 (en) 2021-12-27 2024-05-21 Molecular Devices (Austria) GmbH Organoid microplate
US12544754B2 (en) 2022-01-18 2026-02-10 Molecular Devices (Austria) GmbH 3D printing of organoid passaging plate
US12360102B2 (en) 2022-01-28 2025-07-15 Glaxosmithkline Intellectual Property Development Limited High-throughput assay for cell migration, chemotaxis, and function
CN114591836A (zh) * 2022-02-18 2022-06-07 清华大学深圳国际研究生院 体外血管床微流控芯片及其应用
EP4493670A1 (en) * 2022-03-18 2025-01-22 Molecular Devices (Austria) GmbH Cell culture application methods of using a separation well microplate
WO2023183734A1 (en) * 2022-03-21 2023-09-28 Molecular Devices (Austria) GmbH Systems and methods for co-culture in microplates
WO2023218024A1 (en) * 2022-05-13 2023-11-16 Ecole Polytechnique Federale De Lausanne (Epfl) System for automated and dynamic cell culture
US20230383225A1 (en) * 2022-05-27 2023-11-30 University Of Utah Research Foundation Lung-on-chip device with integrated extracellular matrix membrane
DE102022209416B3 (de) * 2022-09-09 2023-12-21 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidische Vorrichtung
WO2024129942A1 (en) * 2022-12-14 2024-06-20 President And Fellows Of Harvard College Organoids in extracellular matrix droplets
DE102023200837A1 (de) * 2023-02-02 2024-08-08 Carl Zeiss Ag Probengefäß zur Kultivierung biologischer Proben, Vorrichtung zu dessen Betrieb und Mikroskop
WO2024182622A1 (en) * 2023-03-01 2024-09-06 Research Institute At Nationwide Children's Hospital Systems, devices, and processes for miniaturized physiological mimicry
NL2034328B1 (en) 2023-03-13 2024-09-24 Mimetas B V Liver vasculature
WO2024191299A1 (en) 2023-03-13 2024-09-19 Mimetas B.V. Liver vasculature
CN117586876B (zh) * 2023-10-07 2024-10-25 丹望医疗科技(上海)有限公司 一种血管化类器官芯片及血管化类器官
WO2025080829A1 (en) * 2023-10-10 2025-04-17 The Trustees Of The University Of Pennsylvania A model of the blood-brain barrier on a chip
AU2024402761A1 (en) * 2023-12-21 2026-04-30 F. Hoffmann-La Roche Ag Cell culturing device
WO2025186340A1 (de) * 2024-03-05 2025-09-12 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Vorrichtung zum erzeugen von drei-dimensionalen zellstrukturen in einem hydrogel, mikrosystem und/oder mikrofluidische zellkulturplattform und verfahren zum herstellen der vorrichtung
CN118374350A (zh) * 2024-04-15 2024-07-23 丹望医疗科技(上海)有限公司 血管化类器官芯片及其应用
CN118086053B (zh) * 2024-04-29 2024-07-09 四川迪亚生物科技集团有限公司 一种自灌流培养板及使用方法
TR2024007044A1 (tr) * 2024-06-04 2025-12-22 Izmir Yueksek Teknoloji Enstituesue Rektoerluegue 2 ve 3 boyutlu hücre ve doku kültürünleri̇nde kullanilmak üzere üstü açik bi̇r mi̇krofi̇zyoloji̇k si̇stem ve bu si̇stemi̇n çalişma yöntemi̇
GB202409293D0 (en) 2024-06-27 2024-08-14 Momo Biotech Ltd Device
CN120591192A (zh) * 2024-07-15 2025-09-05 苏州先觉生物科技有限公司 一种基于牺牲材料的3d细胞培养体的制备方法、3d细胞培养体及应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010149292A1 (en) * 2009-06-16 2010-12-29 Universiteit Leiden A biological microfluidics chip and related methods
WO2013085909A1 (en) * 2011-12-05 2013-06-13 Research Triangle Institute Human conducting airway model comprising multiple fluidic pathways
WO2016077277A1 (en) * 2014-11-10 2016-05-19 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Modular microtube network for vascularized organ-on-a-chip models
WO2016081751A1 (en) * 2014-11-19 2016-05-26 Nortis, Inc. Method for vascularizing in-vitro generated or ex-vivo tissue fragments in a microfluidic device

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829000A (en) 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
US7534601B2 (en) * 2002-08-27 2009-05-19 Vanderbilt University Capillary perfused bioreactors with multiple chambers
GB0321158D0 (en) * 2003-09-10 2003-10-08 Central Research Lab Ltd Apparatus and method for handling cells,embryos or oocytes
EP1904619A4 (en) 2005-07-07 2012-05-02 Univ California METHOD AND DEVICE FOR CELL CULTURE ARRAY
US20110129850A1 (en) 2006-12-22 2011-06-02 UCLA Office of Intellectual Property Microfluidic platform for cell culture and assay
US8114289B2 (en) 2008-01-29 2012-02-14 California Institute Of Technology Method and apparatus for microfiltration to perform cell separation
WO2009130694A2 (en) * 2008-04-21 2009-10-29 Cell Kinetics Ltd. Flat cell carriers with cell traps
RU2517046C2 (ru) 2008-06-04 2014-05-27 Тиссюз Гмбх Устройство "орган-на-чипе"
DK3404093T3 (da) * 2008-07-16 2020-03-16 Childrens Medical Center Anordning og fremgangsmåde til overvågning af celleadfærd
CN102112594A (zh) * 2008-08-01 2011-06-29 智能生物系统公司 细胞培养系统的样品端口
GB0815427D0 (en) * 2008-08-22 2008-10-01 Capsant Neurotechnologies S A Device
US9126199B2 (en) 2008-09-22 2015-09-08 Universitat Zurich Prorektorat Forschung Hanging drop plate
WO2010062911A2 (en) 2008-11-26 2010-06-03 Hurel Corporation Compositions and methods of functionally enhanced in vitro cell culture system
EP2213364A1 (en) 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Phase guide patterns for liquid manipulation
JP2010253572A (ja) 2009-04-21 2010-11-11 Makita Corp 切断機における割り刃の固定具脱落防止構造
JP6170431B2 (ja) 2010-09-29 2017-07-26 マサチューセッツ インスティテュート オブ テクノロジー 細胞間相互作用のハイスループット研究のためのデバイス
EP2681306B1 (en) * 2011-02-28 2019-01-09 President and Fellows of Harvard College Cell culture system
GB201103917D0 (en) 2011-03-08 2011-04-20 Univ Leiden Apparatus for and methods of processing liquids or liquid based substances
US20140348706A1 (en) * 2011-10-24 2014-11-27 Agency For Science, Technology And Research Microfluidic Device and Microfluidic System
US20130267961A1 (en) * 2012-03-09 2013-10-10 Richard G. Mauldin Impactor
SG10201607963SA (en) 2012-04-01 2016-11-29 Emd Millipore Corp Cell culture and gradient migration assay method and devices
GB2505706A (en) 2012-09-10 2014-03-12 Univ Leiden Apparatus comprising meniscus alignment barriers
US10012640B2 (en) * 2013-02-14 2018-07-03 Cfd Research Corporation Cell culture device with an array of microfluidic networks
EP2811012A1 (en) 2013-06-07 2014-12-10 Eidgenossisch Technische Hochschule Zürich Hanging network plate
CN114134042A (zh) * 2013-09-05 2022-03-04 伯尔尼大学 用于体内器官组织的体外模型化的装置
GB2583047B (en) 2013-12-20 2021-03-24 Harvard College Organomimetic devices and methods of use and manufacturing thereof
RU2584598C1 (ru) * 2015-03-27 2016-05-20 Общество с ограниченной ответственностью Научно-технический центр "БиоКлиникум" Микрофлюидный чип для создания клеточных моделей органов млекопитающих
CN104877905A (zh) 2015-05-04 2015-09-02 清华大学深圳研究生院 细胞体外共培养微流控芯片、系统及方法
NL2014840B1 (en) * 2015-05-21 2017-01-31 Intecrypt B V Microfluidic device for in vitro 3D cell culture experimentation.
WO2016195480A1 (en) 2015-06-05 2016-12-08 Mimetas B.V. Microfluidic plate

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010149292A1 (en) * 2009-06-16 2010-12-29 Universiteit Leiden A biological microfluidics chip and related methods
WO2013085909A1 (en) * 2011-12-05 2013-06-13 Research Triangle Institute Human conducting airway model comprising multiple fluidic pathways
WO2016077277A1 (en) * 2014-11-10 2016-05-19 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Modular microtube network for vascularized organ-on-a-chip models
WO2016081751A1 (en) * 2014-11-19 2016-05-26 Nortis, Inc. Method for vascularizing in-vitro generated or ex-vivo tissue fragments in a microfluidic device

Also Published As

Publication number Publication date
RU2019100603A (ru) 2020-07-15
US11629319B2 (en) 2023-04-18
WO2017216113A3 (en) 2018-02-01
EP3472301C0 (en) 2023-08-02
JP6967535B2 (ja) 2021-11-17
EP3472301A2 (en) 2019-04-24
CN109804057B (zh) 2022-09-09
US20200063081A1 (en) 2020-02-27
JP2019517808A (ja) 2019-06-27
BR112018075920A2 (pt) 2019-03-26
CA3027616A1 (en) 2017-12-21
RU2019100603A3 (enExample) 2020-08-12
CN109804057A (zh) 2019-05-24
AU2017286096A1 (en) 2019-01-17
RU2741806C2 (ru) 2021-01-28
EP3472301B1 (en) 2023-08-02
WO2017216113A2 (en) 2017-12-21

Similar Documents

Publication Publication Date Title
US11629319B2 (en) Cell culture device and methods
NL2022085B1 (en) Device for assessing mechanical strain induced in or by cells
Wang et al. In vitro generation of colonic epithelium from primary cells guided by microstructures
Wang et al. Capture and 3D culture of colonic crypts and colonoids in a microarray platform
WO2009126524A2 (en) Three-dimensional microfluidic platforms and methods of use thereof
EP4148117A1 (en) Cell culture apparatus, methods for cell cultivation by using the same, cell culture incubator comprising the same, and uses of the cell culture apparatus
AU2022289996B2 (en) Method and apparatus for forming a microfluidic gel structure
Wang et al. 3D anastomosed microvascular network model with living capillary networks and endothelial cell-lined microfluidic channels
WO2023073178A1 (en) Microfluidic cell culturing device
US20260117161A1 (en) Cell culture apparatus, methods for cell cultivation by using the same, cell culture incubator comprising the same, and uses of the cell culture apparatus
NL2016965B1 (en) Cell culture device.
US20230250377A1 (en) Microfluidic cell culture device
US20260109925A1 (en) Microfluidic device for forming three-dimensional tissue barrier
NL2035770B1 (en) Titerplate
CN117957303A (zh) 细胞培养装置、使用该细胞培养装置的细胞培养方法、包括该细胞培养装置的细胞培养孵箱以及该细胞培养装置的用途
IT202100025460A1 (it) Dispositivo lab-on-chip per studiare la migrazione cellulare in sistemi tridimensionali e relativo metodo di utilizzo
Wang et al. 3D anastomosed microvascular network model with living capillary

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)