AU2017258523B2 - Method of Nucleic Acid sequence determination - Google Patents

Method of Nucleic Acid sequence determination Download PDF

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Publication number
AU2017258523B2
AU2017258523B2 AU2017258523A AU2017258523A AU2017258523B2 AU 2017258523 B2 AU2017258523 B2 AU 2017258523B2 AU 2017258523 A AU2017258523 A AU 2017258523A AU 2017258523 A AU2017258523 A AU 2017258523A AU 2017258523 B2 AU2017258523 B2 AU 2017258523B2
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AU
Australia
Prior art keywords
nucleotide
nucleic acid
polymerase
dna polymerase
optionally
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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AU2017258523A
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English (en)
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AU2017258523A1 (en
Inventor
Pinar IYIDOGAN
Kandaswamy Vijayan
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Pacific Biosciences of California Inc
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Pacific Biosciences of California Inc
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Assigned to PACIFIC BIOSCIENCES OF CALIFORNIA, INC. reassignment PACIFIC BIOSCIENCES OF CALIFORNIA, INC. Request for Assignment Assignors: Omniome, Inc.
Ceased legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Kinetics & Catalysis (AREA)
AU2017258523A 2016-04-29 2017-04-28 Method of Nucleic Acid sequence determination Ceased AU2017258523B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662329489P 2016-04-29 2016-04-29
US62/329,489 2016-04-29
US201762444733P 2017-01-10 2017-01-10
US62/444,733 2017-01-10
PCT/US2017/030135 WO2017190012A1 (en) 2016-04-29 2017-04-28 Method of nucleic acid sequence determination

Publications (2)

Publication Number Publication Date
AU2017258523A1 AU2017258523A1 (en) 2018-11-22
AU2017258523B2 true AU2017258523B2 (en) 2020-08-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017258523A Ceased AU2017258523B2 (en) 2016-04-29 2017-04-28 Method of Nucleic Acid sequence determination

Country Status (9)

Country Link
US (2) US10584379B2 (OSRAM)
EP (1) EP3449011B1 (OSRAM)
JP (1) JP6915939B2 (OSRAM)
KR (1) KR102231977B1 (OSRAM)
CN (1) CN109154024B (OSRAM)
AU (1) AU2017258523B2 (OSRAM)
CA (1) CA3022431C (OSRAM)
SG (1) SG11201809522WA (OSRAM)
WO (1) WO2017190012A1 (OSRAM)

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US10975427B2 (en) 2017-01-20 2021-04-13 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
WO2019161381A1 (en) 2018-02-16 2019-08-22 Illumina, Inc. Charge-tagged nucleotides and methods of use thereof
EP4234718A3 (en) 2018-04-26 2023-11-29 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
IL278769B2 (en) 2018-05-17 2025-06-01 Stuart Lindsay Device, system and method for direct electrical measurement of enzyme activity
AU2019276719B2 (en) 2018-05-31 2025-11-13 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
CN112567048A (zh) 2018-07-24 2021-03-26 欧姆尼欧美公司 三元复合物种类的连续形成
US10731141B2 (en) 2018-09-17 2020-08-04 Omniome, Inc. Engineered polymerases for improved sequencing
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
WO2020117653A1 (en) 2018-12-04 2020-06-11 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
US11041199B2 (en) 2018-12-20 2021-06-22 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
EP4269604A3 (en) 2019-01-30 2024-03-20 Arizona Board of Regents on behalf of Arizona State University Bioelectronic circuits, systems and methods for preparing and using them
EP3924513B1 (en) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
US12313627B2 (en) 2019-05-01 2025-05-27 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
WO2020242901A1 (en) * 2019-05-24 2020-12-03 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
AU2020294800A1 (en) * 2019-06-20 2022-02-10 Arizona Board Of Regents On Behalf Of Arizona State University Direct electrical readout of nucleic acid sequences
EP3986907A1 (en) * 2019-06-21 2022-04-27 Thermo Fisher Scientific Baltics UAB Oligonucleotide-tethered triphosphate nucleotides useful for labelling nucleic acids for preparing next-generation sequencing libraries
US11180520B2 (en) 2019-09-10 2021-11-23 Omniome, Inc. Reversible modifications of nucleotides
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
CN118186063A (zh) 2019-10-18 2024-06-14 加利福尼亚太平洋生物科学股份有限公司 用于对核酸加帽的方法和组合物
WO2021092431A1 (en) 2019-11-08 2021-05-14 Omniome, Inc. Engineered polymerases for improved sequencing by binding
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
AU2021230282A1 (en) 2020-03-03 2022-09-22 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
EP4146822A1 (en) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
AU2021299216A1 (en) * 2020-06-30 2022-12-08 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless DNA
EP4337789A1 (en) 2021-05-10 2024-03-20 Pacific Biosciences of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
WO2022240764A1 (en) 2021-05-10 2022-11-17 Pacific Biosciences Of California, Inc. Single-molecule seeding and amplification on a surface
AU2021463802A1 (en) * 2021-09-07 2024-03-28 Mgi Tech Co., Ltd. Method for analyzing sequence of target polynucleotide
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
WO2025244989A1 (en) 2024-05-21 2025-11-27 Pacific Biosciences Of California, Inc. Selectively functionalized nanoscale wells

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Also Published As

Publication number Publication date
EP3449011A1 (en) 2019-03-06
JP6915939B2 (ja) 2021-08-11
KR20190003660A (ko) 2019-01-09
US20200199668A1 (en) 2020-06-25
KR102231977B1 (ko) 2021-03-24
JP2019526225A (ja) 2019-09-19
US20170314072A1 (en) 2017-11-02
CA3022431C (en) 2023-03-21
CN109154024A (zh) 2019-01-04
EP3449011B1 (en) 2020-11-04
AU2017258523A1 (en) 2018-11-22
SG11201809522WA (en) 2018-11-29
CA3022431A1 (en) 2017-11-02
WO2017190012A1 (en) 2017-11-02
CN109154024B (zh) 2022-03-04
US10584379B2 (en) 2020-03-10
US11976323B2 (en) 2024-05-07

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FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.

Free format text: FORMER OWNER(S): OMNIOME, INC.

MK14 Patent ceased section 143(a) (annual fees not paid) or expired