AU2017246827B2 - Microfluidic siphoning array for nucleic acid quantification - Google Patents

Microfluidic siphoning array for nucleic acid quantification Download PDF

Info

Publication number
AU2017246827B2
AU2017246827B2 AU2017246827A AU2017246827A AU2017246827B2 AU 2017246827 B2 AU2017246827 B2 AU 2017246827B2 AU 2017246827 A AU2017246827 A AU 2017246827A AU 2017246827 A AU2017246827 A AU 2017246827A AU 2017246827 B2 AU2017246827 B2 AU 2017246827B2
Authority
AU
Australia
Prior art keywords
microchambers
microchannel
microfluidic device
thin film
less
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2017246827A
Other languages
English (en)
Other versions
AU2017246827A1 (en
Inventor
Megan Dueck
Ju-Sung HUNG
Andrew Zayac
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Combinati Inc
Original Assignee
Combinati Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Combinati Inc filed Critical Combinati Inc
Publication of AU2017246827A1 publication Critical patent/AU2017246827A1/en
Application granted granted Critical
Publication of AU2017246827B2 publication Critical patent/AU2017246827B2/en
Priority to AU2022202227A priority Critical patent/AU2022202227B2/en
Priority to AU2024205417A priority patent/AU2024205417A1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Fluid Mechanics (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micromachines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
AU2017246827A 2016-04-04 2017-04-04 Microfluidic siphoning array for nucleic acid quantification Active AU2017246827B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2022202227A AU2022202227B2 (en) 2016-04-04 2022-04-01 Microfluidic siphoning array for nucleic acid quantification
AU2024205417A AU2024205417A1 (en) 2016-04-04 2024-08-02 Microfluidic siphoning array for nucleic acid quantification

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662317993P 2016-04-04 2016-04-04
US62/317,993 2016-04-04
US15/363,896 US9845499B2 (en) 2016-04-04 2016-11-29 Microfluidic siphoning array for nucleic acid quantification
US15/363,896 2016-11-29
PCT/US2017/025873 WO2017176699A1 (en) 2016-04-04 2017-04-04 Microfluidic siphoning array for nucleic acid quantification

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2022202227A Division AU2022202227B2 (en) 2016-04-04 2022-04-01 Microfluidic siphoning array for nucleic acid quantification

Publications (2)

Publication Number Publication Date
AU2017246827A1 AU2017246827A1 (en) 2018-10-18
AU2017246827B2 true AU2017246827B2 (en) 2022-01-06

Family

ID=59958599

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2017246827A Active AU2017246827B2 (en) 2016-04-04 2017-04-04 Microfluidic siphoning array for nucleic acid quantification
AU2022202227A Active AU2022202227B2 (en) 2016-04-04 2022-04-01 Microfluidic siphoning array for nucleic acid quantification
AU2024205417A Pending AU2024205417A1 (en) 2016-04-04 2024-08-02 Microfluidic siphoning array for nucleic acid quantification

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2022202227A Active AU2022202227B2 (en) 2016-04-04 2022-04-01 Microfluidic siphoning array for nucleic acid quantification
AU2024205417A Pending AU2024205417A1 (en) 2016-04-04 2024-08-02 Microfluidic siphoning array for nucleic acid quantification

Country Status (8)

Country Link
US (1) US9845499B2 (https=)
EP (2) EP3439779B1 (https=)
JP (2) JP6970448B2 (https=)
KR (4) KR20260003197A (https=)
CN (2) CN109310980B (https=)
AU (3) AU2017246827B2 (https=)
CA (1) CA3019385A1 (https=)
WO (1) WO2017176699A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11285478B2 (en) 2016-04-04 2022-03-29 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
US9845499B2 (en) * 2016-04-04 2017-12-19 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
AU2017363076B2 (en) 2016-11-17 2021-04-29 Combinati Incorporated Methods and systems for nucleic acid analysis and quantification
CN110997900B (zh) * 2017-07-14 2024-06-14 多伦多大学管理委员会 用于快速产生用于化合物筛选的类器官/球状体的微流体平台
EP3774052A4 (en) * 2018-04-04 2022-01-05 Combinati Incorporated MICROFLUIDIC SUCTION ARRANGEMENT FOR NUCLEIC ACID QUANTIFICATION
AU2019397371B2 (en) 2018-12-10 2025-09-18 Combinati Incorporated Microfluidic array for sample digitization
BR112022014571A2 (pt) * 2020-01-24 2022-10-04 Combinati Incorporated Métodos e sistemas para fabricação de dispositivo microfluídico
CN116368213A (zh) * 2020-09-28 2023-06-30 康比纳蒂股份有限公司 用于样品处理的装置和方法
CN114644976A (zh) * 2020-12-18 2022-06-21 湖南乐准智芯生物科技有限公司 一种微反应室阵列结构及进样方法
WO2023043848A1 (en) 2021-09-14 2023-03-23 Combinati, Inc. Microfluidic device and method for processing biological samples
CN115093962B (zh) * 2022-08-24 2023-03-24 翊新诊断技术(苏州)有限公司 基于柔性薄膜的微流控芯片及其在核酸扩增中的应用
US20240198336A1 (en) 2022-12-19 2024-06-20 Anchorline Biolabs, Inc. Capillary partitioning microfluidics

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030190608A1 (en) * 1999-11-12 2003-10-09 Gary Blackburn Microfluidic devices comprising biochannels
US20050009101A1 (en) * 2001-05-17 2005-01-13 Motorola, Inc. Microfluidic devices comprising biochannels

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US6977145B2 (en) * 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6569674B1 (en) * 1999-12-15 2003-05-27 Amersham Biosciences Ab Method and apparatus for performing biological reactions on a substrate surface
US6361958B1 (en) * 1999-11-12 2002-03-26 Motorola, Inc. Biochannel assay for hybridization with biomaterial
AU2082701A (en) * 1999-12-09 2001-06-18 Motorola, Inc. Multilayered microfluidic devices for analyte reactions
JP2002018271A (ja) 2000-07-05 2002-01-22 Kawamura Inst Of Chem Res 微小ケミカルデバイス
WO2004040001A2 (en) 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US7445926B2 (en) 2002-12-30 2008-11-04 The Regents Of The University Of California Fluid control structures in microfluidic devices
US20050226779A1 (en) 2003-09-19 2005-10-13 Oldham Mark F Vacuum assist for a microplate
WO2005080606A1 (en) * 2004-02-18 2005-09-01 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
JP2005329333A (ja) * 2004-05-20 2005-12-02 Japan Science & Technology Agency マイクロ脱気デバイス
WO2006116455A2 (en) 2005-04-26 2006-11-02 Applera Corporation System for genetic surveillance and analysis
KR100785016B1 (ko) * 2006-05-22 2007-12-12 삼성전자주식회사 단일 마이크로 챔버에서 핵산의 농축 및 증폭을 수행하는방법 및 장치
GB2445738A (en) * 2007-01-16 2008-07-23 Lab901 Ltd Microfluidic device
JP2009236555A (ja) * 2008-03-26 2009-10-15 Shimadzu Corp 流体デバイス及びその製造方法
US20090250347A1 (en) 2008-04-03 2009-10-08 Protea Biosciences, Inc. Microfluidic devices & processes for electrokinetic transport
KR20120063162A (ko) 2010-12-07 2012-06-15 삼성전자주식회사 유전자 분석 장치 및 이를 이용한 유전자 분석 방법
EP3236260B1 (en) * 2011-04-13 2020-09-30 Akonni Biosystems, Inc. Method for making a microarray assembly
EP2761304A4 (en) * 2011-09-30 2015-01-28 Univ California MICROFLUIDIC APPARATUS AND METHOD FOR EXAMINING A LIQUID SAMPLE THEREWITH
US9738887B2 (en) 2012-02-13 2017-08-22 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
ITTO20120703A1 (it) 2012-08-03 2014-02-04 Biomerieux Sa Dispositivo microfluidico monouso, cartuccia includente il dispositivo microfluidico, apparecchio per eseguire una amplificazione di acido nucleico, metodo di fabbricazione del dispositivo microfluidico, e metodo di uso del dispositivo microfluidico
DE102013205412A1 (de) 2013-03-27 2014-10-02 Robert Bosch Gmbh Normal-geschlossenes Ventil für mikrofluidische Bauteile aus einem polymeren Schichtsystem sowie Verfahren
EP3077806B1 (en) 2013-12-06 2019-09-11 Ortho Clinical Diagnostics, Inc. Assay device having a wash port
WO2015085171A1 (en) 2013-12-06 2015-06-11 Adventist Health Systems/Sunbelt, Inc. Role of mcp-1 in relapse of acute myeloid leukemia after hematopoietic stem cell transplantation
KR101562318B1 (ko) * 2014-02-10 2015-10-22 나노바이오시스 주식회사 미세유체 칩 및 이를 이용한 실시간 분석 장치
CN204727866U (zh) * 2014-03-31 2015-10-28 博奥生物集团有限公司 一种集核酸扩增和微阵列检测于一体的微流控装置
KR101486413B1 (ko) * 2014-08-06 2015-01-26 경희대학교 산학협력단 마이크로 포스트를 이용한 미세유동 칩 및 그 제조방법
CN104877905A (zh) * 2015-05-04 2015-09-02 清华大学深圳研究生院 细胞体外共培养微流控芯片、系统及方法
CN104894106A (zh) * 2015-05-23 2015-09-09 浙江大学 高集成度等距等分核酸扩增微流控芯片及应用
US9845499B2 (en) * 2016-04-04 2017-12-19 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030190608A1 (en) * 1999-11-12 2003-10-09 Gary Blackburn Microfluidic devices comprising biochannels
US20050009101A1 (en) * 2001-05-17 2005-01-13 Motorola, Inc. Microfluidic devices comprising biochannels

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
XU JIE et al., "Use of a porous membrane for gas bubble removal in microfluidic channels: physical mechanisms and design criteria", (2010), vol. 9, pages 765-772. *

Also Published As

Publication number Publication date
CN115232707A (zh) 2022-10-25
CA3019385A1 (en) 2017-10-12
EP4512527A3 (en) 2025-04-16
KR102438312B1 (ko) 2022-09-01
US9845499B2 (en) 2017-12-19
US20170283855A1 (en) 2017-10-05
JP7528048B2 (ja) 2024-08-05
KR20240010561A (ko) 2024-01-23
EP4512527A2 (en) 2025-02-26
AU2022202227A1 (en) 2022-04-21
KR20180131601A (ko) 2018-12-10
EP3439779A1 (en) 2019-02-13
EP3439779B1 (en) 2024-11-13
AU2022202227B2 (en) 2024-05-02
CN109310980A (zh) 2019-02-05
JP2019515660A (ja) 2019-06-13
KR102631090B1 (ko) 2024-01-29
AU2024205417A1 (en) 2024-10-24
AU2017246827A1 (en) 2018-10-18
KR20260003197A (ko) 2026-01-06
KR20220122799A (ko) 2022-09-02
EP3439779A4 (en) 2019-11-27
KR102893830B1 (ko) 2025-12-03
JP6970448B2 (ja) 2021-11-24
CN109310980B (zh) 2022-07-19
WO2017176699A1 (en) 2017-10-12
JP2022028671A (ja) 2022-02-16

Similar Documents

Publication Publication Date Title
US12551890B2 (en) Microfluidic siphoning array for nucleic acid quantification
AU2022202227B2 (en) Microfluidic siphoning array for nucleic acid quantification
AU2021206903C1 (en) Methods and systems for nucleic acid analysis and Quantification
US20230035727A1 (en) Methods and systems for microfluidic device manufacturing
US20210197202A1 (en) Microfluidic siphoning array for nucleic acid quantification

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired
NA Applications received for extensions of time, section 223

Free format text: AN APPLICATION TO EXTEND THE TIME FROM 04 APR 2023 TO 04 DEC 2023 IN WHICH TO PAY A RENEWAL FEE HAS BEEN FILED

NB Applications allowed - extensions of time section 223(2)

Free format text: THE TIME IN WHICH TO PAY A RENEWAL FEE HAS BEEN EXTENDED TO 04 DEC 2023