AU2017216864B2 - Method for the production of immunoglobulin single variable domains - Google Patents

Method for the production of immunoglobulin single variable domains Download PDF

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Publication number
AU2017216864B2
AU2017216864B2 AU2017216864A AU2017216864A AU2017216864B2 AU 2017216864 B2 AU2017216864 B2 AU 2017216864B2 AU 2017216864 A AU2017216864 A AU 2017216864A AU 2017216864 A AU2017216864 A AU 2017216864A AU 2017216864 B2 AU2017216864 B2 AU 2017216864B2
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leu
ala
gly
glu
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AU2017216864A
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AU2017216864A1 (en
Inventor
Manu De Groeve
Peter Schotte
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Ablynx NV
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Ablynx NV
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AU2017216864A 2016-02-12 2017-02-10 Method for the production of immunoglobulin single variable domains Active AU2017216864B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2023233070A AU2023233070A1 (en) 2016-02-12 2023-09-19 Method for the production of immunoglobulin single variable domains

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662294470P 2016-02-12 2016-02-12
US62/294,470 2016-02-12
PCT/EP2017/053034 WO2017137579A1 (en) 2016-02-12 2017-02-10 Method for the production of immunoglobulin single variable domains

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2023233070A Division AU2023233070A1 (en) 2016-02-12 2023-09-19 Method for the production of immunoglobulin single variable domains

Publications (2)

Publication Number Publication Date
AU2017216864A1 AU2017216864A1 (en) 2018-07-26
AU2017216864B2 true AU2017216864B2 (en) 2023-06-22

Family

ID=58018106

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2017216864A Active AU2017216864B2 (en) 2016-02-12 2017-02-10 Method for the production of immunoglobulin single variable domains
AU2023233070A Abandoned AU2023233070A1 (en) 2016-02-12 2023-09-19 Method for the production of immunoglobulin single variable domains

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2023233070A Abandoned AU2023233070A1 (en) 2016-02-12 2023-09-19 Method for the production of immunoglobulin single variable domains

Country Status (7)

Country Link
US (2) US10975141B2 (enExample)
EP (1) EP3414268A1 (enExample)
JP (2) JP7540877B2 (enExample)
CN (1) CN108699151B (enExample)
AU (2) AU2017216864B2 (enExample)
CA (1) CA3014443A1 (enExample)
WO (1) WO2017137579A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO2768984T3 (enExample) * 2015-11-12 2018-06-09
EP3414268A1 (en) 2016-02-12 2018-12-19 Ablynx NV Method for the production of immunoglobulin single variable domains
EP3778865A4 (en) 2018-03-26 2021-12-22 National University Corporation Kobe University Novel cell and method for making a protein of interest using it
EP4069733A1 (en) * 2019-12-06 2022-10-12 Ablynx N.V. POLYPEPTIDES COMPRISING IMMUNOGLOBULIN SINGLE VARIABLE DOMAINS TARGETING TNFalpha AND IL-23
CN110964113B (zh) * 2019-12-24 2021-09-03 北京纽安博生物技术有限公司 介导结合免疫球蛋白的单域抗体、由其构建的双功能抗体及其应用
JP7714233B2 (ja) * 2020-03-19 2025-07-29 国立大学法人神戸大学 内在性遺伝子の発現が増強された遺伝子組換え細胞の製造方法
MX2022012278A (es) * 2020-03-30 2022-10-27 Ablynx Nv Metodo para la produccion y purificacion de dominios variables unicos de inmunoglobulina multivalentes.
FR3120877B1 (fr) 2021-03-19 2025-03-14 Univ Toulouse 3 Paul Sabatier Cellule immunitaire comprenant un récepteur Fc à sa surface, et sur lequel est greffé une molécule hybride comprenant un fragment Fc d’anticorps et au moins un peptide citrulliné dérivé de la fibrine, et ses utilisations
FR3120866B1 (fr) 2021-03-19 2025-02-28 Univ Toulouse 3 Paul Sabatier Molécule hybride comprenant un peptide citrulliné dérivé de la fibrine et un anticorps ou fragment d’anticorps se liant à CD38 et/ou CD138, et ses utilisations
FR3120865B1 (fr) 2021-03-19 2025-02-28 Univ Toulouse 3 Paul Sabatier Molécule hybride comprenant un fragment Fc d’anticorps et au moins un peptide citrulliné dérivé de la fibrine, et ses utilisations
CN114920839B (zh) * 2022-06-17 2023-03-10 天津大学 特异性结合il-23的纳米抗体及其用途

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2022855B1 (en) 2006-05-16 2015-08-19 Daiichi Sankyo Company, Limited High secretion production method of protein
US9212226B2 (en) 2008-05-16 2015-12-15 Ablynx N.V. Amino acid sequences directed against CXCR4 and other GPCRs and compounds comprising the same
CA2759370C (en) 2009-04-30 2020-02-11 Peter Schotte Method for the production of domain antibodies
US8440456B2 (en) * 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
WO2011161266A1 (en) 2010-06-25 2011-12-29 Ablynx Nv Improved immunoglobulin single variable domains and constructs thereof directed against cxcr4
AU2012234284B2 (en) * 2011-03-28 2015-10-08 Ablynx Nv Bispecific anti-CXCR7 immunoglobulin single variable domains
LT3248986T (lt) 2014-05-16 2022-03-25 Ablynx Nv Imunoglobulino kintami domenai
EP3414268A1 (en) 2016-02-12 2018-12-19 Ablynx NV Method for the production of immunoglobulin single variable domains

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
K.E. ROYLE: "Modelling as a tool for increasing the specific productivity of single-chain antibody fragments from Pichia pastoris", 2013-09-01, <URL:https://spiral.imperial.ac.uk/bitstream/10044/1/24959/1/Kate Elizabeth Royle 060414.pdf> *

Also Published As

Publication number Publication date
WO2017137579A1 (en) 2017-08-17
CA3014443A1 (en) 2017-08-17
US20190194302A1 (en) 2019-06-27
AU2023233070A1 (en) 2023-10-05
JP2019507748A (ja) 2019-03-22
CN108699151B (zh) 2022-08-12
US10975141B2 (en) 2021-04-13
JP2024073531A (ja) 2024-05-29
US11851480B2 (en) 2023-12-26
JP7540877B2 (ja) 2024-08-27
AU2017216864A1 (en) 2018-07-26
EP3414268A1 (en) 2018-12-19
CN108699151A (zh) 2018-10-23
US20210047392A1 (en) 2021-02-18
JP7849405B2 (ja) 2026-04-21

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