CN108699151B - 用于制备免疫球蛋白单可变结构域的方法 - Google Patents

用于制备免疫球蛋白单可变结构域的方法 Download PDF

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CN108699151B
CN108699151B CN201780010990.9A CN201780010990A CN108699151B CN 108699151 B CN108699151 B CN 108699151B CN 201780010990 A CN201780010990 A CN 201780010990A CN 108699151 B CN108699151 B CN 108699151B
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nucleic acid
single variable
pichia
polypeptide
immunoglobulin single
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CN108699151A (zh
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彼得·肖特
马努·德格罗依夫
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Ablynx NV
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201780010990.9A 2016-02-12 2017-02-10 用于制备免疫球蛋白单可变结构域的方法 Active CN108699151B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662294470P 2016-02-12 2016-02-12
US62/294,470 2016-02-12
PCT/EP2017/053034 WO2017137579A1 (en) 2016-02-12 2017-02-10 Method for the production of immunoglobulin single variable domains

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Publication Number Publication Date
CN108699151A CN108699151A (zh) 2018-10-23
CN108699151B true CN108699151B (zh) 2022-08-12

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US (2) US10975141B2 (enExample)
EP (1) EP3414268A1 (enExample)
JP (2) JP7540877B2 (enExample)
CN (1) CN108699151B (enExample)
AU (2) AU2017216864B2 (enExample)
CA (1) CA3014443A1 (enExample)
WO (1) WO2017137579A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO2768984T3 (enExample) * 2015-11-12 2018-06-09
EP3414268A1 (en) 2016-02-12 2018-12-19 Ablynx NV Method for the production of immunoglobulin single variable domains
WO2019187911A1 (ja) * 2018-03-26 2019-10-03 国立大学法人神戸大学 新規細胞及びそれを用いた目的タンパク質の製造方法
CN114981300B (zh) * 2019-12-06 2025-10-31 艾伯霖克斯公司 包含靶向TNFα和IL-23的免疫球蛋白单个可变结构域的多肽
CN110964113B (zh) * 2019-12-24 2021-09-03 北京纽安博生物技术有限公司 介导结合免疫球蛋白的单域抗体、由其构建的双功能抗体及其应用
WO2021187560A1 (ja) * 2020-03-19 2021-09-23 国立大学法人神戸大学 内在性遺伝子の発現が増強された遺伝子組換え細胞の製造方法
EP4126971A1 (en) 2020-03-30 2023-02-08 Ablynx N.V. Method for the production and purification of multivalent immunoglobulin single variable domains
FR3120866B1 (fr) 2021-03-19 2025-02-28 Univ Toulouse 3 Paul Sabatier Molécule hybride comprenant un peptide citrulliné dérivé de la fibrine et un anticorps ou fragment d’anticorps se liant à CD38 et/ou CD138, et ses utilisations
FR3120877B1 (fr) 2021-03-19 2025-03-14 Univ Toulouse 3 Paul Sabatier Cellule immunitaire comprenant un récepteur Fc à sa surface, et sur lequel est greffé une molécule hybride comprenant un fragment Fc d’anticorps et au moins un peptide citrulliné dérivé de la fibrine, et ses utilisations
FR3120865B1 (fr) 2021-03-19 2025-02-28 Univ Toulouse 3 Paul Sabatier Molécule hybride comprenant un fragment Fc d’anticorps et au moins un peptide citrulliné dérivé de la fibrine, et ses utilisations
CN114920839B (zh) * 2022-06-17 2023-03-10 天津大学 特异性结合il-23的纳米抗体及其用途

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2022855A1 (en) * 2006-05-16 2009-02-11 Kirin Pharma Kabushiki Kaisha High secretion production method of protein
WO2010135678A1 (en) * 2009-05-22 2010-11-25 Research Corporation Technologies, Inc. Nucleic acids of pichia pastoris and use thereof for recombinant production of proteins
CN103917560A (zh) * 2011-03-28 2014-07-09 埃博灵克斯股份有限公司 双特异性抗-cxcr7免疫球蛋白单可变结构域

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9212226B2 (en) 2008-05-16 2015-12-15 Ablynx N.V. Amino acid sequences directed against CXCR4 and other GPCRs and compounds comprising the same
DK2982690T3 (da) 2009-04-30 2021-04-12 Ablynx Nv Fremgangsmåde til fremstilling af domæne-antistoffer
WO2011161266A1 (en) 2010-06-25 2011-12-29 Ablynx Nv Improved immunoglobulin single variable domains and constructs thereof directed against cxcr4
PT3248986T (pt) 2014-05-16 2022-04-05 Ablynx Nv Domínios variáveis de imunoglobulina
EP3414268A1 (en) 2016-02-12 2018-12-19 Ablynx NV Method for the production of immunoglobulin single variable domains

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2022855A1 (en) * 2006-05-16 2009-02-11 Kirin Pharma Kabushiki Kaisha High secretion production method of protein
WO2010135678A1 (en) * 2009-05-22 2010-11-25 Research Corporation Technologies, Inc. Nucleic acids of pichia pastoris and use thereof for recombinant production of proteins
CN103917560A (zh) * 2011-03-28 2014-07-09 埃博灵克斯股份有限公司 双特异性抗-cxcr7免疫球蛋白单可变结构域

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Engineering of Pichia pastoris for Improved Production of Antibody Fragments;Brigitte Gasser 等;《Biotechnology and Bioengineering》;20060605;第94卷(第2期);摘要、第358页右栏第1段、图6、第360页右栏第4段 *
Fusion of a Short Peptide that Binds Immunoglobulin G to a Recombinant Protein Substantially Increases Its Plasma Half-Life in Mice;Jonathan T. Sockolosky 等;《PLOS ONE》;20140724;第9卷(第7期);摘要 *
Modelling as a tool for increasing the specific productivity of single-chain antibody fragments from Pichia pastoris;Kate Elizabeth Royle;《A thesis submitted for the degree of Doctor of Philosophy of Imperial College London and the Diploma of Imperial College London》;20130930;摘要 *
The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins;Mouna Guerfal1 等;《Microbial Cell Factories》;20101231;第9卷;摘要、图2 *

Also Published As

Publication number Publication date
AU2017216864A1 (en) 2018-07-26
CA3014443A1 (en) 2017-08-17
WO2017137579A1 (en) 2017-08-17
JP2019507748A (ja) 2019-03-22
AU2023233070A1 (en) 2023-10-05
US11851480B2 (en) 2023-12-26
US20210047392A1 (en) 2021-02-18
EP3414268A1 (en) 2018-12-19
CN108699151A (zh) 2018-10-23
JP2024073531A (ja) 2024-05-29
AU2017216864B2 (en) 2023-06-22
JP7540877B2 (ja) 2024-08-27
US10975141B2 (en) 2021-04-13
US20190194302A1 (en) 2019-06-27

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