AU2016389495B2 - Systems and methods for in vivo dual recombinase-mediated cassette exchange (dRMCE) and disease models thereof - Google Patents

Systems and methods for in vivo dual recombinase-mediated cassette exchange (dRMCE) and disease models thereof Download PDF

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AU2016389495B2
AU2016389495B2 AU2016389495A AU2016389495A AU2016389495B2 AU 2016389495 B2 AU2016389495 B2 AU 2016389495B2 AU 2016389495 A AU2016389495 A AU 2016389495A AU 2016389495 A AU2016389495 A AU 2016389495A AU 2016389495 B2 AU2016389495 B2 AU 2016389495B2
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rna
cells
transgene
promoter
recombinase
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AU2016389495A1 (en
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Joshua BREUNIG
Moise Danielpour
Gi Bum Kim
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Cedars Sinai Medical Center
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

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AU2016389495A 2016-01-26 2016-12-30 Systems and methods for in vivo dual recombinase-mediated cassette exchange (dRMCE) and disease models thereof Active AU2016389495B2 (en)

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US201662287197P 2016-01-26 2016-01-26
US62/287,197 2016-01-26
PCT/US2016/069442 WO2017131926A1 (en) 2016-01-26 2016-12-30 SYSTEMS AND METHODS FOR IN VIVO DUAL RECOMBINASE-MEDIATED CASSETTE EXCHANGE (dRMCE) AND DISEASE MODELS THEREOF

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AU2016389495B2 true AU2016389495B2 (en) 2023-03-30

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US (2) US11466290B2 (https=)
EP (1) EP3408396A4 (https=)
JP (3) JP2019511243A (https=)
KR (1) KR102811061B1 (https=)
CN (1) CN108884472A (https=)
AU (1) AU2016389495B2 (https=)
CA (1) CA3012042A1 (https=)
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JP2019511243A (ja) 2016-01-26 2019-04-25 シーダーズ−サイナイ メディカル センター インビボのデュアルリコンビナーゼ媒介性カセット交換（ｄＲＭＣＥ）のためのシステム及び方法ならびにその疾患モデル
US12258571B2 (en) 2018-03-16 2025-03-25 Cedars-Sinai Medical Center Methods and compositions for inducible expression of neurotrophic factors
CN109321599B (zh) * 2018-09-27 2023-08-22 同济大学 人多能干细胞中的谱系示踪系统的构建与应用
CN109943564B (zh) * 2019-03-28 2022-03-15 上海市中医老年医学研究所 Amh基因定点敲入2A-Cre的杂合子小鼠模型构建方法及其应用
CA3143981A1 (en) * 2019-06-17 2020-12-24 Cedars-Sinai Medical Center Systems and methods for in vivo dual recombinase-mediated cassette exchange (drmce) and disease models thereof
CN118547004A (zh) * 2019-06-19 2024-08-27 豪夫迈·罗氏有限公司 通过使用Cre mRNA进行的靶向整合来产生蛋白质表达细胞的方法
US20250354186A1 (en) 2022-06-06 2025-11-20 Kyowa Hakko Bio Co., Ltd. Microorganism having improved 3'-sialyllactose productivity and production method of 3'-sialyllactose

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EP3408396A1 (en) 2018-12-05
KR20180101535A (ko) 2018-09-12
US20230084201A1 (en) 2023-03-16
AU2016389495A1 (en) 2018-08-16
JP2019511243A (ja) 2019-04-25
WO2017131926A1 (en) 2017-08-03
KR102811061B1 (ko) 2025-05-21
EP3408396A4 (en) 2019-10-09
CA3012042A1 (en) 2017-08-03
CN108884472A (zh) 2018-11-23
US11466290B2 (en) 2022-10-11
JP2022091801A (ja) 2022-06-21
JP2024063065A (ja) 2024-05-10
US20190390222A1 (en) 2019-12-26

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