AU2016357478B2 - Methods of identifying immune cells in PD-L1 positive tumor tissue - Google Patents

Methods of identifying immune cells in PD-L1 positive tumor tissue Download PDF

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AU2016357478B2
AU2016357478B2 AU2016357478A AU2016357478A AU2016357478B2 AU 2016357478 B2 AU2016357478 B2 AU 2016357478B2 AU 2016357478 A AU2016357478 A AU 2016357478A AU 2016357478 A AU2016357478 A AU 2016357478A AU 2016357478 B2 AU2016357478 B2 AU 2016357478B2
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enzyme
detection probe
detection
dye
tissue sample
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AU2016357478A1 (en
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Chandler Morgan BIRCH
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Ventana Medical Systems Inc
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    • G—PHYSICS
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2812—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD4
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    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
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    • C07K16/289—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD45
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    • C07K16/2896—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
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    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
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    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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    • G01N2035/00099—Characterised by type of test elements
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    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
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AU2016357478A 2015-11-22 2016-11-21 Methods of identifying immune cells in PD-L1 positive tumor tissue Active AU2016357478B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562258493P 2015-11-22 2015-11-22
US62/258,493 2015-11-22
PCT/EP2016/078237 WO2017085307A1 (en) 2015-11-22 2016-11-21 Methods of identifying immune cells in pd-l1 positive tumor tissue

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AU2016357478A1 AU2016357478A1 (en) 2018-05-10
AU2016357478B2 true AU2016357478B2 (en) 2023-07-06

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US (1) US11899016B2 (https=)
EP (1) EP3377530B1 (https=)
JP (1) JP6832930B2 (https=)
AU (1) AU2016357478B2 (https=)
CA (1) CA3005804C (https=)
ES (1) ES2886600T3 (https=)
WO (1) WO2017085307A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102389677B1 (ko) 2013-03-15 2022-04-21 제넨테크, 인크. Pd-1 및 pd-l1 관련 상태를 치료하기 위한 바이오마커 및 방법
ES2916923T3 (es) * 2014-07-11 2022-07-06 Ventana Med Syst Inc Anticuerpos anti-PD-L1 y usos diagnósticos de los mismos
EP3596114A2 (en) 2017-03-16 2020-01-22 Alpine Immune Sciences, Inc. Cd80 variant immunomodulatory proteins and uses thereof
US12065476B2 (en) 2018-06-15 2024-08-20 Alpine Immune Sciences, Inc. PD-1 variant immunomodulatory proteins and uses thereof
AU2019345151B2 (en) 2018-09-19 2025-04-17 Alpine Immune Sciences, Inc. Methods and uses of variant CD80 fusion proteins and related constructs
JP7259019B2 (ja) 2018-09-20 2023-04-17 ヴェンタナ メディカル システムズ， インク． フローサイトメトリデータを分析するためのサイズに基づくゲーティング
CN112789684B (zh) 2018-10-01 2025-05-09 文塔纳医疗系统公司 用于预测对pd-1轴导向疗法的应答的方法和系统
CN113383089A (zh) 2018-11-29 2021-09-10 文塔纳医疗系统公司 经由代表性DNA测序进行个性化ctDNA疾病监测
WO2021224293A1 (en) * 2020-05-07 2021-11-11 Ventana Medical Systems, Inc. Histochemical systems and methods for evaluating egfr and egfr ligand expression in tumor samples
CN111830256A (zh) * 2020-07-01 2020-10-27 北京基谱生物科技有限公司 一种石蜡切片免疫荧光多重染色试剂盒及使用方法
JPWO2022059601A1 (https=) * 2020-09-16 2022-03-24
CN118786346A (zh) * 2022-03-07 2024-10-15 阿斯利康(瑞典)有限公司 用于预测患者对免疫疗法的应答的方法
WO2023172883A1 (en) 2022-03-07 2023-09-14 Alpine Immune Sciences, Inc. Immunomodulatory proteins of variant cd80 polypeptides, cell therapies thereof and related methods and uses
JP2025526652A (ja) * 2022-08-08 2025-08-15 ヴェンタナ メディカル システムズ， インク． デジタル化された病理スライドの定量化のためのシステムおよび方法
CN121368715A (zh) 2023-04-13 2026-01-20 文塔纳医疗系统公司 用于固定的实体瘤的增殖测定
WO2025193604A1 (en) 2024-03-11 2025-09-18 Ventana Medical Systems, Inc. Non-enzymatic dissociation of ffpe tissue and generation of single cells with intact cell surface markers

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA01004287A (es) * 1998-10-26 2002-06-04 Ventana Med Syst Inc Deteccion del virus del papiloma humano en frotis de papanicolau (pap).
US6544798B1 (en) 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6195451B1 (en) * 1999-05-13 2001-02-27 Advanced Pathology Ststems, Inc. Transformation of digital images
US8071322B2 (en) * 2002-11-12 2011-12-06 Epitomics, Inc. Method for identifying differentially expressed proteins
WO2012003476A2 (en) * 2010-07-02 2012-01-05 Ventana Medical Systems, Inc. Hapten conjugates for target detection
EP2831587B1 (en) * 2012-03-27 2018-05-16 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
CN113967253A (zh) * 2012-05-15 2022-01-25 百时美施贵宝公司 通过破坏pd-1/pd-l1信号传输的免疫治疗
WO2014194293A1 (en) * 2013-05-30 2014-12-04 Amplimmune, Inc. Improved methods for the selection of patients for pd-1 or b7-h4 targeted therapies, and combination therapies thereof
AU2014293154A1 (en) * 2013-07-24 2016-02-18 Dana-Farber Cancer Institute, Inc. Anti-galectin-1 monoclonal antibodies and fragments thereof
EP3079772B1 (en) * 2013-12-10 2020-02-05 Merck Sharp & Dohme Corp. Immunohistochemical proximity assay for pd-1 positive cells and pd-ligand positive cells in tumor tissue
WO2015124703A1 (en) * 2014-02-24 2015-08-27 Ventana Medical Systems, Inc. Quinone methide analog signal amplification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
B. J. CHEN ET AL: "PD-L1 Expression Is Characteristic of a Subset of Aggressive B-cell Lymphomas and Virus-Associated Malignancies", CLINICAL CANCER RESEARCH, vol. 19, no. 13, 14 May 2013, pages 3462 - 3473 *

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JP2018536163A (ja) 2018-12-06
EP3377530B1 (en) 2021-06-30
AU2016357478A1 (en) 2018-05-10
ES2886600T3 (es) 2021-12-20
EP3377530A1 (en) 2018-09-26
CA3005804C (en) 2022-03-29
JP6832930B2 (ja) 2021-02-24
US20180372747A1 (en) 2018-12-27
CA3005804A1 (en) 2017-05-26
WO2017085307A1 (en) 2017-05-26
US11899016B2 (en) 2024-02-13

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