AU2016258980B2 - Potency assay - Google Patents

Potency assay Download PDF

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Publication number
AU2016258980B2
AU2016258980B2 AU2016258980A AU2016258980A AU2016258980B2 AU 2016258980 B2 AU2016258980 B2 AU 2016258980B2 AU 2016258980 A AU2016258980 A AU 2016258980A AU 2016258980 A AU2016258980 A AU 2016258980A AU 2016258980 B2 AU2016258980 B2 AU 2016258980B2
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AU
Australia
Prior art keywords
cells
mpc
culture medium
mpcs
human
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AU2016258980A
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AU2016258980A1 (en
Inventor
Fiona SEE
Paul Simmons
Colby SUIRE
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Mesoblast International SARL
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Mesoblast International SARL
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Priority claimed from AU2015901605A external-priority patent/AU2015901605A0/en
Application filed by Mesoblast International SARL filed Critical Mesoblast International SARL
Publication of AU2016258980A1 publication Critical patent/AU2016258980A1/en
Application granted granted Critical
Publication of AU2016258980B2 publication Critical patent/AU2016258980B2/en
Priority to AU2022203140A priority Critical patent/AU2022203140B2/en
Priority to AU2025205043A priority patent/AU2025205043A1/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/495—Transforming growth factor [TGF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/10—Musculoskeletal or connective tissue disorders

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Epidemiology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Rheumatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
AU2016258980A 2015-05-05 2016-05-04 Potency assay Active AU2016258980B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2022203140A AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2015901605 2015-05-05
AU2015901605A AU2015901605A0 (en) 2015-05-05 Potency assay
PCT/EP2016/060049 WO2016177805A1 (en) 2015-05-05 2016-05-04 Potency assay

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2022203140A Division AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay

Publications (2)

Publication Number Publication Date
AU2016258980A1 AU2016258980A1 (en) 2017-11-23
AU2016258980B2 true AU2016258980B2 (en) 2022-02-17

Family

ID=56087237

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2016258980A Active AU2016258980B2 (en) 2015-05-05 2016-05-04 Potency assay
AU2022203140A Active AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A Pending AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Family Applications After (2)

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AU2022203140A Active AU2022203140B2 (en) 2015-05-05 2022-05-11 Potency assay
AU2025205043A Pending AU2025205043A1 (en) 2015-05-05 2025-07-02 Potency assay

Country Status (9)

Country Link
US (3) US11795435B2 (cg-RX-API-DMAC7.html)
EP (2) EP3292199B1 (cg-RX-API-DMAC7.html)
JP (4) JP6797833B2 (cg-RX-API-DMAC7.html)
KR (2) KR102885856B1 (cg-RX-API-DMAC7.html)
CN (2) CN107771220B (cg-RX-API-DMAC7.html)
AU (3) AU2016258980B2 (cg-RX-API-DMAC7.html)
CA (1) CA2984987C (cg-RX-API-DMAC7.html)
ES (2) ES2911266T3 (cg-RX-API-DMAC7.html)
WO (1) WO2016177805A1 (cg-RX-API-DMAC7.html)

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WO2019102268A1 (en) 2017-11-22 2019-05-31 Mesoblast International Sarl Cellular compositions and methods of treatment i
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EP4772245A3 (en) * 2019-01-02 2026-08-19 Mesoblast International Sàrl Method for treating lower back pain
US12702683B2 (en) 2019-01-03 2026-08-11 Mesoblast International Sárl Method for improving visual acuity
WO2021176001A1 (en) 2020-03-05 2021-09-10 Mesoblast International Sàrl Method for treating inflammatory lung diseases using mesenchymal lineage precursor or stem cells
CA3180808A1 (en) * 2020-04-20 2021-10-28 Longeveron Inc. Potency assay
US20260209709A1 (en) * 2022-12-23 2026-07-23 Kangstem Biotech Co., Ltd. Method for selecting stem cells with enhanced cartilage differentiation capability and cell therapy product comprising same

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Also Published As

Publication number Publication date
AU2022203140A1 (en) 2022-06-02
JP2018517900A (ja) 2018-07-05
CN107771220A (zh) 2018-03-06
KR20230140604A (ko) 2023-10-06
KR102885856B1 (ko) 2025-11-14
AU2025205043A1 (en) 2025-07-24
US20240409898A1 (en) 2024-12-12
EP4015624B1 (en) 2025-02-19
CA2984987A1 (en) 2016-11-10
JP7335222B2 (ja) 2023-08-29
JP2023159260A (ja) 2023-10-31
ES3018944T3 (en) 2025-05-19
CA2984987C (en) 2023-10-10
AU2016258980A1 (en) 2017-11-23
JP2021036892A (ja) 2021-03-11
BR112017023660A2 (pt) 2018-07-17
HK1247236A1 (zh) 2018-09-21
CN107771220B (zh) 2022-07-05
CN115141872A (zh) 2022-10-04
JP6797833B2 (ja) 2020-12-09
WO2016177805A1 (en) 2016-11-10
EP4015624A1 (en) 2022-06-22
JP2026012704A (ja) 2026-01-27
US20240076621A1 (en) 2024-03-07
KR102582602B1 (ko) 2023-09-25
JP7754893B2 (ja) 2025-10-15
KR20180041621A (ko) 2018-04-24
US20200140822A1 (en) 2020-05-07
ES2911266T3 (es) 2022-05-18
EP3292199A1 (en) 2018-03-14
US11795435B2 (en) 2023-10-24
EP3292199B1 (en) 2022-01-12
AU2022203140B2 (en) 2025-04-10

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