AU2016233648B2 - A three-component crispr/cas complex system and uses thereof - Google Patents

A three-component crispr/cas complex system and uses thereof Download PDF

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AU2016233648B2
AU2016233648B2 AU2016233648A AU2016233648A AU2016233648B2 AU 2016233648 B2 AU2016233648 B2 AU 2016233648B2 AU 2016233648 A AU2016233648 A AU 2016233648A AU 2016233648 A AU2016233648 A AU 2016233648A AU 2016233648 B2 AU2016233648 B2 AU 2016233648B2
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sequence
puf
protein
polynucleotide
cas9
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AU2016233648A1 (en
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Albert Cheng
Nathaniel JILLETTE
Haoyi WANG
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Jackson Laboratory
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
    • C12N9/224—Class 1 CAS enzyme complex, e.g. multi-enzyme
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
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AU2016233648A 2015-03-13 2016-03-09 A three-component crispr/cas complex system and uses thereof Active AU2016233648B2 (en)

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US201562132644P 2015-03-13 2015-03-13
US62/132,644 2015-03-13
US201562221249P 2015-09-21 2015-09-21
US62/221,249 2015-09-21
PCT/US2016/021491 WO2016148994A1 (en) 2015-03-13 2016-03-09 A three-component crispr/cas complex system and uses thereof

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EP (2) EP3858992A1 (https=)
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CN (1) CN107922942B (https=)
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CA (1) CA2979567C (https=)
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IL (2) IL254428B (https=)
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CA2979567C (en) 2020-10-13
JP6588995B2 (ja) 2019-10-09
SG11201707393SA (en) 2017-10-30
WO2016148994A1 (en) 2016-09-22
ES2886901T3 (es) 2021-12-21
CA3091771A1 (en) 2016-09-22
HK1249545A1 (en) 2018-11-02
JP2019176877A (ja) 2019-10-17
EP3268472A1 (en) 2018-01-17
SG10202008443UA (en) 2020-10-29
IL276894A (en) 2020-10-29
HK1254126A1 (zh) 2019-07-12
JP6867440B2 (ja) 2021-04-28
JP2018507713A (ja) 2018-03-22
KR20170124601A (ko) 2017-11-10
IL276894B (en) 2022-12-01
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