AU2015264357B2 - Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides - Google Patents

Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides Download PDF

Info

Publication number
AU2015264357B2
AU2015264357B2 AU2015264357A AU2015264357A AU2015264357B2 AU 2015264357 B2 AU2015264357 B2 AU 2015264357B2 AU 2015264357 A AU2015264357 A AU 2015264357A AU 2015264357 A AU2015264357 A AU 2015264357A AU 2015264357 B2 AU2015264357 B2 AU 2015264357B2
Authority
AU
Australia
Prior art keywords
subsequence
sequence
target
blocker
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2015264357A
Other languages
English (en)
Other versions
AU2015264357A1 (en
Inventor
Juexiao WANG
Ruojia WU
David Yu Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
William Marsh Rice University
Original Assignee
William Marsh Rice University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by William Marsh Rice University filed Critical William Marsh Rice University
Publication of AU2015264357A1 publication Critical patent/AU2015264357A1/en
Application granted granted Critical
Publication of AU2015264357B2 publication Critical patent/AU2015264357B2/en
Priority to AU2021266291A priority Critical patent/AU2021266291C1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2015264357A 2014-05-19 2015-05-19 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides Active AU2015264357B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2021266291A AU2021266291C1 (en) 2014-05-19 2021-11-11 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462000114P 2014-05-19 2014-05-19
US62/000,114 2014-05-19
PCT/US2015/031476 WO2015179339A1 (en) 2014-05-19 2015-05-19 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2021266291A Division AU2021266291C1 (en) 2014-05-19 2021-11-11 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Publications (2)

Publication Number Publication Date
AU2015264357A1 AU2015264357A1 (en) 2017-01-05
AU2015264357B2 true AU2015264357B2 (en) 2021-08-12

Family

ID=54554607

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2015264357A Active AU2015264357B2 (en) 2014-05-19 2015-05-19 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
AU2021266291A Active AU2021266291C1 (en) 2014-05-19 2021-11-11 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2021266291A Active AU2021266291C1 (en) 2014-05-19 2021-11-11 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Country Status (13)

Country Link
US (2) US20170067090A1 (OSRAM)
EP (3) EP3901278B1 (OSRAM)
JP (4) JP2017515484A (OSRAM)
KR (1) KR102343605B1 (OSRAM)
CN (2) CN114032234A (OSRAM)
AU (2) AU2015264357B2 (OSRAM)
BR (1) BR112016027063A2 (OSRAM)
CA (1) CA2985631A1 (OSRAM)
DK (1) DK3146080T3 (OSRAM)
ES (1) ES2861353T3 (OSRAM)
PH (1) PH12016502542B1 (OSRAM)
RU (1) RU2708992C2 (OSRAM)
WO (1) WO2015179339A1 (OSRAM)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
WO2015077717A1 (en) 2013-11-25 2015-05-28 The Broad Institute Inc. Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
WO2015085147A1 (en) 2013-12-05 2015-06-11 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
EP3082853A2 (en) 2013-12-20 2016-10-26 The Broad Institute, Inc. Combination therapy with neoantigen vaccine
EP3234193B1 (en) 2014-12-19 2020-07-15 Massachusetts Institute of Technology Molecular biomarkers for cancer immunotherapy
ES3049406T3 (en) 2014-12-19 2025-12-16 Broad Inst Inc Methods for profiling the t-cell-receptor repertoire
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
US10280455B2 (en) 2015-12-30 2019-05-07 Bio-Rad Laboratories, Inc. Split-cycle and tape amplification
CN109563506A (zh) 2016-05-06 2019-04-02 威廉马歇莱思大学 使用随机捕获探针文库的化学计量核酸纯化
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
KR102522023B1 (ko) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11549149B2 (en) * 2017-01-24 2023-01-10 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
KR102790050B1 (ko) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 단일 세포를 위한 샘플 인덱싱
JP2021500077A (ja) * 2017-10-18 2021-01-07 デイ ゼロ ダイアグノスティックス， インコーポレイテッド Ｄｎａ増幅の標的化抑制による混合ｄｎａ試料中のｄｎａ集団の選択的富化
WO2019164885A1 (en) * 2018-02-20 2019-08-29 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
CN108220444A (zh) * 2018-02-28 2018-06-29 无锡禾盛医疗器械有限公司 一种kras基因突变检测的引物探针组合及其应用
EP4545647A3 (en) 2018-05-03 2025-07-09 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP4471156A3 (en) 2018-10-01 2025-02-26 Becton, Dickinson and Company Determining 5' transcript sequences
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
WO2020123384A1 (en) * 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
EP3911754A4 (en) * 2019-01-15 2022-10-12 Tangen Biosciences Inc. METHOD FOR REMOVAL OF NON-SPECIFIC AMPLIFICATION PRODUCTS IN NUCLEIC ACID AMPLIFICATION TECHNOLOGIES
ES2989967T3 (es) 2019-01-23 2024-11-28 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
WO2020259678A1 (zh) * 2019-06-26 2020-12-30 南京金斯瑞生物科技有限公司 一种含有阻断物的寡核苷酸
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
US20240279735A1 (en) * 2019-12-20 2024-08-22 Korea University Research And Business Foundation Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
KR20230028450A (ko) * 2020-06-26 2023-02-28 윌리엄 마쉬 라이스 유니버시티 앰플리콘 포괄적 풍부화
CN111647650B (zh) * 2020-07-06 2023-06-27 河南赛诺特生物技术有限公司 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN116547390A (zh) * 2020-11-02 2023-08-04 阅尔基因美国公司 定量多重扩增子测序系统
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN112522369A (zh) * 2020-12-08 2021-03-19 合肥欧创基因生物科技有限公司 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
WO2022146773A1 (en) 2020-12-29 2022-07-07 Nuprobe Usa, Inc. Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta)
WO2022155397A1 (en) 2021-01-15 2022-07-21 Nuprobe Usa, Inc. Cycle multiplexing for highly multiplexed quantitative pcr
WO2022197688A1 (en) 2021-03-16 2022-09-22 Nuprobe Usa, Inc. High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection
WO2023077121A1 (en) * 2021-11-01 2023-05-04 Nuprobe Usa, Inc. Rna quantitative amplicon sequencing for gene expression quantitation
CN119662835B (zh) * 2025-02-20 2025-05-27 宁波熙宁检测技术有限公司 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130149695A1 (en) * 2010-04-27 2013-06-13 Samsung Life Public Welfare Foundation Method for detecting genetic mutation by using a blocking primer
WO2013097173A1 (en) * 2011-12-30 2013-07-04 Mackay Memorial Hospital Method and primer set for detecting mutation

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
CN101076608B (zh) * 2004-10-18 2013-04-17 布兰迪斯大学 在pcr扩增中提高再现性和降低引物错导的试剂和方法
US20100009355A1 (en) * 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
EP2207896B1 (en) * 2007-10-04 2014-01-29 Commonwealth Scientific and Industrial Research Organisation Nucleic acid amplification
ES2527653T3 (es) * 2008-10-20 2015-01-28 F. Hoffmann-La Roche Ag Amplificación específica de alelo usando un cebador con un nucleótido modificado
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
WO2010111682A2 (en) * 2009-03-27 2010-09-30 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US20130059294A1 (en) * 2010-05-16 2013-03-07 Xiangdong Ren Identification of polymorphic hepatitis b viruses and kras oncogene mutations and clinical use
CA2824223A1 (en) * 2011-01-14 2012-07-19 Genefirst Limited Methods, compositions, and kits for determining the presence/absence of a variant nucleic acid sequence
CN103635593B (zh) * 2011-05-04 2018-03-20 生物概念股份有限公司 用于检测核酸序列变体的方法
EP2841598B1 (en) * 2012-04-25 2018-03-14 Ho, Tho Huu Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物
JP6652693B2 (ja) * 2013-04-29 2020-02-26 キアゲン ゲーエムベーハー ブロッキングオリゴヌクレオチドを用いたｄｎａ増幅の方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130149695A1 (en) * 2010-04-27 2013-06-13 Samsung Life Public Welfare Foundation Method for detecting genetic mutation by using a blocking primer
WO2013097173A1 (en) * 2011-12-30 2013-07-04 Mackay Memorial Hospital Method and primer set for detecting mutation

Also Published As

Publication number Publication date
AU2021266291A1 (en) 2021-12-09
CN114032234A (zh) 2022-02-11
ES2861353T3 (es) 2021-10-06
AU2021266291B2 (en) 2024-09-19
KR20170003695A (ko) 2017-01-09
EP3901278A1 (en) 2021-10-27
EP3146080A1 (en) 2017-03-29
US20170067090A1 (en) 2017-03-09
CN106661627B (zh) 2021-11-23
CA2985631A1 (en) 2015-11-26
PH12016502542A1 (en) 2017-04-10
EP4512908A3 (en) 2025-03-26
BR112016027063A2 (pt) 2017-10-31
JP7126718B2 (ja) 2022-08-29
US20220090168A1 (en) 2022-03-24
EP3146080B1 (en) 2021-01-06
JP2017515484A (ja) 2017-06-15
DK3146080T3 (en) 2021-04-12
PH12016502542B1 (en) 2024-01-17
WO2015179339A1 (en) 2015-11-26
EP4512908A2 (en) 2025-02-26
AU2021266291C1 (en) 2024-12-12
EP3146080A4 (en) 2017-11-01
JP2021019607A (ja) 2021-02-18
RU2708992C2 (ru) 2019-12-12
RU2016149307A3 (OSRAM) 2018-12-10
AU2015264357A1 (en) 2017-01-05
EP3901278B1 (en) 2024-10-23
RU2016149307A (ru) 2018-06-20
JP2022166131A (ja) 2022-11-01
JP2024167406A (ja) 2024-12-03
KR102343605B1 (ko) 2021-12-27
CN106661627A (zh) 2017-05-10

Similar Documents

Publication Publication Date Title
AU2021266291C1 (en) Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
EP2207896A1 (en) Nucleic acid amplification
US7618773B2 (en) Headloop DNA amplification
WO2013119827A1 (en) Direct rna capture with molecular inversion probes
CN114717298A (zh) 一种多重扩增方法
EP3377652B1 (en) A method and a kit for nucleic acid detection
US11180798B2 (en) Modified primers for nucleic acid amplification and detection
HK40065933A (en) Allele-specific amplification using overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
HK1236578A1 (en) Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
HK1236578B (zh) 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸的组合物进行等位基因特异性扩增
CN119842874B (zh) 非靶标依赖的检测靶基因中snp位点基因型的方法及其试剂盒
EP3377651B1 (en) A method and a kit for nucleic acid detection
Whitcombe 6 Using Scorpion Primers for Genotyping

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)