JP2017515484A5 - - Google Patents
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- JP2017515484A5 JP2017515484A5 JP2016567863A JP2016567863A JP2017515484A5 JP 2017515484 A5 JP2017515484 A5 JP 2017515484A5 JP 2016567863 A JP2016567863 A JP 2016567863A JP 2016567863 A JP2016567863 A JP 2016567863A JP 2017515484 A5 JP2017515484 A5 JP 2017515484A5
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- JP
- Japan
- Prior art keywords
- subsequence
- sequence
- oligonucleotide
- blocker
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 108091034117 Oligonucleotide Proteins 0.000 claims 28
- 239000002773 nucleotide Substances 0.000 claims 11
- 125000003729 nucleotide group Chemical group 0.000 claims 11
- 238000000034 method Methods 0.000 claims 7
- 230000000295 complement effect Effects 0.000 claims 6
- 238000009396 hybridization Methods 0.000 claims 6
- 230000007935 neutral effect Effects 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 102000004190 Enzymes Human genes 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 4
- 125000000524 functional group Chemical group 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 3
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 239000005546 dideoxynucleotide Substances 0.000 claims 2
- 102100034343 Integrase Human genes 0.000 claims 1
- 108060004795 Methyltransferase Proteins 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 102000018120 Recombinases Human genes 0.000 claims 1
- 108010091086 Recombinases Proteins 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 235000013367 dietary fats Nutrition 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000010520 ghee Substances 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- -1 nucleoside triphosphates Chemical class 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462000114P | 2014-05-19 | 2014-05-19 | |
| US62/000,114 | 2014-05-19 | ||
| PCT/US2015/031476 WO2015179339A1 (en) | 2014-05-19 | 2015-05-19 | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020165028A Division JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017515484A JP2017515484A (ja) | 2017-06-15 |
| JP2017515484A5 true JP2017515484A5 (OSRAM) | 2018-06-28 |
Family
ID=54554607
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016567863A Pending JP2017515484A (ja) | 2014-05-19 | 2015-05-19 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2020165028A Active JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A Pending JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2024153764A Pending JP2024167406A (ja) | 2014-05-19 | 2024-09-06 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020165028A Active JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A Pending JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2024153764A Pending JP2024167406A (ja) | 2014-05-19 | 2024-09-06 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20170067090A1 (OSRAM) |
| EP (3) | EP3146080B1 (OSRAM) |
| JP (4) | JP2017515484A (OSRAM) |
| KR (1) | KR102343605B1 (OSRAM) |
| CN (2) | CN106661627B (OSRAM) |
| AU (2) | AU2015264357B2 (OSRAM) |
| BR (1) | BR112016027063A2 (OSRAM) |
| CA (1) | CA2985631A1 (OSRAM) |
| DK (1) | DK3146080T3 (OSRAM) |
| ES (1) | ES2861353T3 (OSRAM) |
| PH (1) | PH12016502542B1 (OSRAM) |
| RU (1) | RU2708992C2 (OSRAM) |
| WO (1) | WO2015179339A1 (OSRAM) |
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| CN105705659B (zh) | 2013-08-28 | 2019-11-29 | 贝克顿迪金森公司 | 大规模平行单细胞分析 |
| US10801070B2 (en) | 2013-11-25 | 2020-10-13 | The Broad Institute, Inc. | Compositions and methods for diagnosing, evaluating and treating cancer |
| US11725237B2 (en) | 2013-12-05 | 2023-08-15 | The Broad Institute Inc. | Polymorphic gene typing and somatic change detection using sequencing data |
| CN106456724A (zh) | 2013-12-20 | 2017-02-22 | 博德研究所 | 使用新抗原疫苗的联合疗法 |
| EP3757211B1 (en) | 2014-12-19 | 2025-07-30 | The Broad Institute, Inc. | Methods for profiling the t-cell-receptor repertoire |
| WO2016100975A1 (en) | 2014-12-19 | 2016-06-23 | Massachsetts Institute Ot Technology | Molecular biomarkers for cancer immunotherapy |
| CN107208158B (zh) | 2015-02-27 | 2022-01-28 | 贝克顿迪金森公司 | 空间上可寻址的分子条形编码 |
| JP7508191B2 (ja) | 2015-03-30 | 2024-07-01 | ベクトン・ディキンソン・アンド・カンパニー | コンビナトリアルバーコーディングのための方法および組成物 |
| US11390914B2 (en) | 2015-04-23 | 2022-07-19 | Becton, Dickinson And Company | Methods and compositions for whole transcriptome amplification |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
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| CN109563506A (zh) | 2016-05-06 | 2019-04-02 | 威廉马歇莱思大学 | 使用随机捕获探针文库的化学计量核酸纯化 |
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| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| EP4300099A3 (en) | 2016-09-26 | 2024-03-27 | Becton, Dickinson and Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
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| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
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| EP3759118A4 (en) * | 2018-02-20 | 2022-03-23 | William Marsh Rice University | Systems and methods for allele enrichment using multiplexed blocker displacement amplification |
| CN108220444A (zh) * | 2018-02-28 | 2018-06-29 | 无锡禾盛医疗器械有限公司 | 一种kras基因突变检测的引物探针组合及其应用 |
| CN112243461B (zh) | 2018-05-03 | 2024-07-12 | 贝克顿迪金森公司 | 在相对的转录物末端进行分子条形码化 |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| JP7670607B2 (ja) | 2018-10-01 | 2025-04-30 | ベクトン・ディキンソン・アンド・カンパニー | ５’転写物配列の決定 |
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| CN113195717A (zh) * | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | 单细胞全转录组分析中的选择性延伸 |
| AU2019423311A1 (en) * | 2019-01-15 | 2021-07-15 | Tangen Biosciences Inc. | A method for suppressing non-specific amplification products in nucleic acid amplification technologies |
| EP3914728B1 (en) | 2019-01-23 | 2023-04-05 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| CN113454234B (zh) | 2019-02-14 | 2025-03-18 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| US20220348606A1 (en) * | 2019-06-26 | 2022-11-03 | Nanjing GenScript Biotech Co., Ltd. | Oligonucleotide containing blocker |
| CN114051534B (zh) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
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| US20240279735A1 (en) * | 2019-12-20 | 2024-08-22 | Korea University Research And Business Foundation | Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same |
| WO2021146207A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and rna |
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| EP4172169A4 (en) * | 2020-06-26 | 2025-04-30 | William Marsh Rice University | COMPREHENSIVE AMPLICON ENRICHEMENT |
| CN111647650B (zh) * | 2020-07-06 | 2023-06-27 | 河南赛诺特生物技术有限公司 | 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
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| WO2022094403A1 (en) * | 2020-11-02 | 2022-05-05 | Nuprobe Usa, Inc. | Quantitative multiplex amplicon sequencing system |
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| CN112522369A (zh) * | 2020-12-08 | 2021-03-19 | 合肥欧创基因生物科技有限公司 | 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法 |
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| EP4278009A1 (en) | 2021-01-15 | 2023-11-22 | Nuprobe USA, Inc. | Cycle multiplexing for highly multiplexed quantitative pcr |
| WO2022197688A1 (en) | 2021-03-16 | 2022-09-22 | Nuprobe Usa, Inc. | High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection |
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| CN103215361A (zh) * | 2013-04-18 | 2013-07-24 | 深圳联合医学科技有限公司 | 等位基因变体检测方法、试剂盒及组合物 |
| WO2014177540A1 (en) * | 2013-04-29 | 2014-11-06 | Qiagen Marseille | A method for dna amplification with a blocking oligonucleotide |
-
2015
- 2015-05-19 JP JP2016567863A patent/JP2017515484A/ja active Pending
- 2015-05-19 CN CN201580039304.1A patent/CN106661627B/zh active Active
- 2015-05-19 CA CA2985631A patent/CA2985631A1/en active Pending
- 2015-05-19 EP EP15796383.6A patent/EP3146080B1/en active Active
- 2015-05-19 DK DK15796383.6T patent/DK3146080T3/da active
- 2015-05-19 CN CN202111356156.XA patent/CN114032234A/zh active Pending
- 2015-05-19 PH PH1/2016/502542A patent/PH12016502542B1/en unknown
- 2015-05-19 EP EP21150302.4A patent/EP3901278B1/en active Active
- 2015-05-19 ES ES15796383T patent/ES2861353T3/es active Active
- 2015-05-19 AU AU2015264357A patent/AU2015264357B2/en active Active
- 2015-05-19 KR KR1020167035369A patent/KR102343605B1/ko active Active
- 2015-05-19 RU RU2016149307A patent/RU2708992C2/ru active
- 2015-05-19 BR BR112016027063A patent/BR112016027063A2/pt not_active Application Discontinuation
- 2015-05-19 EP EP24208177.6A patent/EP4512908A3/en active Pending
- 2015-05-19 WO PCT/US2015/031476 patent/WO2015179339A1/en not_active Ceased
-
2016
- 2016-11-18 US US15/355,235 patent/US20170067090A1/en not_active Abandoned
-
2020
- 2020-09-30 JP JP2020165028A patent/JP7126718B2/ja active Active
-
2021
- 2021-11-11 AU AU2021266291A patent/AU2021266291C1/en active Active
- 2021-11-29 US US17/536,204 patent/US20220090168A1/en active Pending
-
2022
- 2022-08-08 JP JP2022126459A patent/JP2022166131A/ja active Pending
-
2024
- 2024-09-06 JP JP2024153764A patent/JP2024167406A/ja active Pending
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| Publication | Publication Date | Title |
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