AU2013312801B2 - Chimeric polypeptides having targeted binding specificity - Google Patents

Chimeric polypeptides having targeted binding specificity Download PDF

Info

Publication number
AU2013312801B2
AU2013312801B2 AU2013312801A AU2013312801A AU2013312801B2 AU 2013312801 B2 AU2013312801 B2 AU 2013312801B2 AU 2013312801 A AU2013312801 A AU 2013312801A AU 2013312801 A AU2013312801 A AU 2013312801A AU 2013312801 B2 AU2013312801 B2 AU 2013312801B2
Authority
AU
Australia
Prior art keywords
leu
ala
val
gly
gln
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
AU2013312801A
Other languages
English (en)
Other versions
AU2013312801A1 (en
Inventor
Carlos F. Barbas Iii
Thomas GAJ
Brian M. Lamb
Andrew Mercer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Institute filed Critical Scripps Research Institute
Publication of AU2013312801A1 publication Critical patent/AU2013312801A1/en
Application granted granted Critical
Publication of AU2013312801B2 publication Critical patent/AU2013312801B2/en
Priority to AU2019200922A priority Critical patent/AU2019200922B2/en
Priority to AU2021240252A priority patent/AU2021240252B2/en
Revoked legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immunology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AU2013312801A 2012-09-04 2013-09-04 Chimeric polypeptides having targeted binding specificity Revoked AU2013312801B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2019200922A AU2019200922B2 (en) 2012-09-04 2019-02-08 Chimeric polypeptides having targeted binding specificity
AU2021240252A AU2021240252B2 (en) 2012-09-04 2021-09-30 Chimeric polypeptides having targeted binding specificity

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261696689P 2012-09-04 2012-09-04
US61/696,689 2012-09-04
US201361753763P 2013-01-17 2013-01-17
US61/753,763 2013-01-17
US201361818364P 2013-05-01 2013-05-01
US61/818,364 2013-05-01
PCT/US2013/058100 WO2014039585A2 (en) 2012-09-04 2013-09-04 Chimeric polypeptides having targeted binding specificity

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2019200922A Division AU2019200922B2 (en) 2012-09-04 2019-02-08 Chimeric polypeptides having targeted binding specificity

Publications (2)

Publication Number Publication Date
AU2013312801A1 AU2013312801A1 (en) 2015-03-26
AU2013312801B2 true AU2013312801B2 (en) 2018-11-15

Family

ID=50237752

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2013312801A Revoked AU2013312801B2 (en) 2012-09-04 2013-09-04 Chimeric polypeptides having targeted binding specificity
AU2019200922A Active AU2019200922B2 (en) 2012-09-04 2019-02-08 Chimeric polypeptides having targeted binding specificity
AU2021240252A Active AU2021240252B2 (en) 2012-09-04 2021-09-30 Chimeric polypeptides having targeted binding specificity

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2019200922A Active AU2019200922B2 (en) 2012-09-04 2019-02-08 Chimeric polypeptides having targeted binding specificity
AU2021240252A Active AU2021240252B2 (en) 2012-09-04 2021-09-30 Chimeric polypeptides having targeted binding specificity

Country Status (12)

Country Link
US (4) US9902962B2 (enExample)
EP (3) EP4148134A1 (enExample)
JP (4) JP6442406B2 (enExample)
KR (3) KR102201867B1 (enExample)
CN (2) CN104781404B (enExample)
AU (3) AU2013312801B2 (enExample)
BR (1) BR112015004696B1 (enExample)
CA (1) CA2883511C (enExample)
DK (1) DK2893022T3 (enExample)
IL (1) IL237442B (enExample)
SG (2) SG10201701675PA (enExample)
WO (1) WO2014039585A2 (enExample)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3199630B1 (en) 2008-09-05 2019-05-08 President and Fellows of Harvard College Continuous directed evolution of proteins and nucleic acids
EP3202903B1 (en) 2010-12-22 2020-02-12 President and Fellows of Harvard College Continuous directed evolution
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
EP4148134A1 (en) * 2012-09-04 2023-03-15 The Scripps Research Institute Chimeric polypeptides having targeted binding specificity
US9708589B2 (en) * 2012-12-18 2017-07-18 Monsanto Technology Llc Compositions and methods for custom site-specific DNA recombinases
US10793867B2 (en) 2013-03-15 2020-10-06 Monsanto Technology, Llc Methods for targeted transgene-integration using custom site-specific DNA recombinases
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
EP3097196B1 (en) 2014-01-20 2019-09-11 President and Fellows of Harvard College Negative selection and stringency modulation in continuous evolution systems
WO2015153889A2 (en) 2014-04-02 2015-10-08 University Of Florida Research Foundation, Incorporated Materials and methods for the treatment of latent viral infection
US10077434B2 (en) 2014-07-30 2018-09-18 Zumutor Biologics Inc. Non-fucosylated protein and methods thereof
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US10920208B2 (en) 2014-10-22 2021-02-16 President And Fellows Of Harvard College Evolution of proteases
US11299729B2 (en) 2015-04-17 2022-04-12 President And Fellows Of Harvard College Vector-based mutagenesis system
US10392674B2 (en) 2015-07-22 2019-08-27 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11524983B2 (en) 2015-07-23 2022-12-13 President And Fellows Of Harvard College Evolution of Bt toxins
US10612011B2 (en) 2015-07-30 2020-04-07 President And Fellows Of Harvard College Evolution of TALENs
US11339408B2 (en) 2015-08-20 2022-05-24 Applied Stemcell, Inc. Nuclease with enhanced efficiency of genome editing
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) * 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
IL306092A (en) 2017-03-23 2023-11-01 Harvard College Nucleic base editors that include nucleic acid programmable DNA binding proteins
WO2018187796A1 (en) * 2017-04-07 2018-10-11 Donald Danforth Plant Science Center Methods for increasing resistance to cotton bacterial blight and plants produced thereby
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11447809B2 (en) 2017-07-06 2022-09-20 President And Fellows Of Harvard College Evolution of tRNA synthetases
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
US12060553B2 (en) 2017-08-25 2024-08-13 President And Fellows Of Harvard College Evolution of BoNT peptidases
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US11624130B2 (en) 2017-09-18 2023-04-11 President And Fellows Of Harvard College Continuous evolution for stabilized proteins
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
WO2019204643A2 (en) 2018-04-18 2019-10-24 Altius Institute For Biomedical Sciences Animal pathogen-derived polypeptides and uses thereof for genetic engineering
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
US11913044B2 (en) 2018-06-14 2024-02-27 President And Fellows Of Harvard College Evolution of cytidine deaminases
WO2020006126A1 (en) 2018-06-27 2020-01-02 Altius Institute For Biomedical Sciences Nucleic acid binding domains and methods of use thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
CN109771403A (zh) * 2019-01-24 2019-05-21 广州医科大学附属第一医院 一种大鼠造影剂肾病模型的构建方法
WO2020191241A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12655182B2 (en) 2019-07-15 2026-06-16 President And Fellows Of Harvard College Evolved botulinum neurotoxins and uses thereof
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
TW202132565A (zh) * 2019-11-01 2021-09-01 美商聖加莫治療股份有限公司 Ｇｉｎ重組酶變異體
US12012433B1 (en) * 2020-02-27 2024-06-18 Integrated Dna Technologies Inc. Expression and purification of Cas enzymes
EP4143324A2 (en) 2020-05-01 2023-03-08 Integrated DNA Technologies, Inc. Lachnospiraceae sp cas12a mutants with enhanced cleavage activity at non-canonical tttt protospacer adjacent motifs
CA3177481A1 (en) 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
US20230313154A1 (en) * 2020-07-17 2023-10-05 Cb Therapeutics, Inc. Prenyltransferase enzymes
KR102265501B1 (ko) * 2020-12-17 2021-06-15 국립해양생물자원관 다중구리 산화효소 펩타이드를 포함하는 구리대사 관련 신경계 질환의 예방 또는 치료용 조성물

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110301073A1 (en) * 2010-05-17 2011-12-08 Sangamo Biosciences, Inc. Novel DNA-binding proteins and uses thereof

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3623230B2 (ja) * 1992-06-11 2005-02-23 ニューヨーク・ユニバーシティ キメラ受容体ポリペプチド，ヒトｈ１３タンパク質，ならびにその使用
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US20050084885A1 (en) 1994-01-18 2005-04-21 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US7277941B2 (en) 1998-03-11 2007-10-02 Commvault Systems, Inc. System and method for providing encryption in a storage network by storing a secured encryption key with encrypted archive data in an archive storage device
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6503717B2 (en) 1999-12-06 2003-01-07 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
US7013219B2 (en) 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6599692B1 (en) 1999-09-14 2003-07-29 Sangamo Bioscience, Inc. Functional genomics using zinc finger proteins
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
AU4940700A (en) 1999-05-28 2000-12-18 Sangamo Biosciences, Inc. Gene switches
WO2002046412A2 (en) 2000-12-07 2002-06-13 Sangamo Biosciences, Inc. Regulation of angiogenesis with zinc finger proteins
US7067317B2 (en) 2000-12-07 2006-06-27 Sangamo Biosciences, Inc. Regulation of angiogenesis with zinc finger proteins
US7273923B2 (en) 2001-01-22 2007-09-25 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
US7067617B2 (en) 2001-02-21 2006-06-27 The Scripps Research Institute Zinc finger binding domains for nucleotide sequence ANN
US20040224385A1 (en) 2001-08-20 2004-11-11 Barbas Carlos F Zinc finger binding domains for cnn
AU2003215094B2 (en) 2002-02-07 2008-05-29 The Scripps Research Institute Zinc finger libraries
US20060211846A1 (en) 2002-02-13 2006-09-21 Barbas Carlos F Iii Zinc finger binding domains for nucleotide sequence ANN
EP2046806A4 (en) * 2006-07-05 2009-10-21 Scripps Research Inst THROUGH DIRECT EVOLUTION FOR CATALYSIS OPTIMIZED, CHIMERIC ZINC FINGER RECOMBINATION
PT2816112T (pt) * 2009-12-10 2018-11-20 Univ Iowa State Res Found Inc Modificação do adn modificada pelo efector tal
CN103210087B (zh) * 2010-09-06 2015-04-29 淡马锡生命科学研究院有限公司 Xa10与AvrXa10之间的分子相互作用
US9499592B2 (en) * 2011-01-26 2016-11-22 President And Fellows Of Harvard College Transcription activator-like effectors
WO2013074999A1 (en) * 2011-11-16 2013-05-23 Sangamo Biosciences, Inc. Modified dna-binding proteins and uses thereof
US9688997B2 (en) * 2011-12-29 2017-06-27 Iowa State University Research Foundation, Inc. Genetically modified plants with resistance to Xanthomonas and other bacterial plant pathogens
EP4148134A1 (en) * 2012-09-04 2023-03-15 The Scripps Research Institute Chimeric polypeptides having targeted binding specificity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110301073A1 (en) * 2010-05-17 2011-12-08 Sangamo Biosciences, Inc. Novel DNA-binding proteins and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
C. MUSSOLINO ET AL, "A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity", NUCLEIC ACIDS RESEARCH, (2011-08-03), vol. 39, no. 21, doi:10.1093/nar/gkr597, ISSN 0305-1048, pages 9283 - 9293 *

Also Published As

Publication number Publication date
CN109517068B (zh) 2023-07-25
WO2014039585A2 (en) 2014-03-13
KR102357105B1 (ko) 2022-02-08
EP2893022A4 (en) 2016-03-02
BR112015004696A2 (pt) 2017-11-21
KR102596125B1 (ko) 2023-10-30
KR20150070120A (ko) 2015-06-24
WO2014039585A3 (en) 2014-05-22
IL237442A0 (en) 2015-04-30
JP7100681B2 (ja) 2022-07-13
CN109517068A (zh) 2019-03-26
CN104781404A (zh) 2015-07-15
KR102201867B1 (ko) 2021-01-12
SG10201701675PA (en) 2017-04-27
KR20220016303A (ko) 2022-02-08
JP6728283B2 (ja) 2020-07-22
US20150284728A1 (en) 2015-10-08
JP2015532654A (ja) 2015-11-12
KR20210008437A (ko) 2021-01-21
AU2013312801A1 (en) 2015-03-26
AU2019200922A1 (en) 2019-02-28
EP3750999B1 (en) 2022-06-29
JP2020178703A (ja) 2020-11-05
EP4148134A1 (en) 2023-03-15
JP7459174B2 (ja) 2024-04-01
US12662677B2 (en) 2026-06-23
AU2021240252B2 (en) 2024-06-06
US20230304022A1 (en) 2023-09-28
BR112015004696B1 (pt) 2022-07-19
CA2883511C (en) 2024-05-07
SG11201501525QA (en) 2015-03-30
EP2893022B1 (en) 2020-05-13
JP2018183180A (ja) 2018-11-22
US11466277B2 (en) 2022-10-11
US20180230474A1 (en) 2018-08-16
DK2893022T3 (da) 2020-07-27
US20210147854A1 (en) 2021-05-20
CA2883511A1 (en) 2014-03-13
US10829766B2 (en) 2020-11-10
AU2019200922B2 (en) 2021-07-08
JP6442406B2 (ja) 2018-12-19
US9902962B2 (en) 2018-02-27
EP3750999A1 (en) 2020-12-16
IL237442B (en) 2019-11-28
AU2021240252A1 (en) 2021-10-28
EP2893022A2 (en) 2015-07-15
CN104781404B (zh) 2018-10-26
JP2022130649A (ja) 2022-09-06

Similar Documents

Publication Publication Date Title
AU2021240252B2 (en) Chimeric polypeptides having targeted binding specificity
US20240229077A1 (en) Methods and compositions for prime editing nucleotide sequences
KR20220052370A (ko) 보편적 공여자 세포
US20210277366A1 (en) ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS
AU2017378482A1 (en) Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
KR20220058579A (ko) 보편적 공여자 세포
US11278570B2 (en) Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
HK40043614A (en) Chimeric polypeptides having targeted binding specificity
HK40043614B (en) Chimeric polypeptides having targeted binding specificity
HK40089287A (en) Chimeric polypeptides having targeted binding specificity
US8759089B2 (en) Methods and systems for high homologous recombination (“HR”) targeting efficiency

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MAK Offer to surrender letters patent
MAL Surrender and revocation of letters patent

Effective date: 20210923