AU2013240248B2 - Delivery of packaged RNA to mammalian cells - Google Patents

Delivery of packaged RNA to mammalian cells Download PDF

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Publication number
AU2013240248B2
AU2013240248B2 AU2013240248A AU2013240248A AU2013240248B2 AU 2013240248 B2 AU2013240248 B2 AU 2013240248B2 AU 2013240248 A AU2013240248 A AU 2013240248A AU 2013240248 A AU2013240248 A AU 2013240248A AU 2013240248 B2 AU2013240248 B2 AU 2013240248B2
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virus
vlp
protein
polynucleotide
rna
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AU2013240248A1 (en
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Deb K. Chatterjee
Stanislaw J. Kaczmarczyk
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US Department of Health and Human Services
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Government of the United States of America
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Priority to AU2019201911A priority Critical patent/AU2019201911A1/en
Priority to AU2021240286A priority patent/AU2021240286B2/en
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/76—Viruses; Subviral particles; Bacteriophages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/177—Receptors; Cell surface antigens; Cell surface determinants
    • A61K38/1774—Immunoglobulin superfamily (e.g. CD2, CD4, CD8, ICAM molecules, B7 molecules, Fc-receptors, MHC-molecules)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N2310/141—MicroRNAs, miRNAs
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    • C12N2740/00—Reverse transcribing RNA viruses
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    • C12N2740/11011—Alpharetrovirus, e.g. avian leucosis virus
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    • C12N2740/10011—Retroviridae
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    • C12N2740/10011—Retroviridae
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    • C12N2740/11041—Use of virus, viral particle or viral elements as a vector
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
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    • C12N2740/10011—Retroviridae
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    • C12N2740/10011—Retroviridae
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    • C12N2770/00011—Details
    • C12N2770/36011—Togaviridae
    • C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
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    • C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36141—Use of virus, viral particle or viral elements as a vector
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AU2013240248A 2012-03-26 2013-03-15 Delivery of packaged RNA to mammalian cells Active AU2013240248B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2019201911A AU2019201911A1 (en) 2012-03-26 2019-03-19 Delivery of packaged RNA to mammalian cells
AU2021240286A AU2021240286B2 (en) 2012-03-26 2021-10-01 Delivery of packaged RNA to mammalian cells

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261615687P 2012-03-26 2012-03-26
US61/615,687 2012-03-26
PCT/US2013/031876 WO2013148302A1 (en) 2012-03-26 2013-03-15 Delivery of packaged rna to mammalian cells

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AU2019201911A Division AU2019201911A1 (en) 2012-03-26 2019-03-19 Delivery of packaged RNA to mammalian cells

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AU2013240248A1 AU2013240248A1 (en) 2014-11-06
AU2013240248B2 true AU2013240248B2 (en) 2018-12-20

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AU2019201911A Abandoned AU2019201911A1 (en) 2012-03-26 2019-03-19 Delivery of packaged RNA to mammalian cells
AU2021240286A Active AU2021240286B2 (en) 2012-03-26 2021-10-01 Delivery of packaged RNA to mammalian cells

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AU2021240286A Active AU2021240286B2 (en) 2012-03-26 2021-10-01 Delivery of packaged RNA to mammalian cells

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US (3) US9506041B2 (cg-RX-API-DMAC7.html)
EP (2) EP3663395B1 (cg-RX-API-DMAC7.html)
JP (2) JP2015527296A (cg-RX-API-DMAC7.html)
AU (3) AU2013240248B2 (cg-RX-API-DMAC7.html)
WO (1) WO2013148302A1 (cg-RX-API-DMAC7.html)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010040023A2 (en) 2008-10-03 2010-04-08 Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Methods and compositions for protein delivery
US10130697B2 (en) 2010-03-23 2018-11-20 Wisconsin Alumni Research Foundation (Warf) Vaccines comprising mutant attenuated influenza viruses
EP2747778B1 (en) 2011-08-26 2017-12-06 Wisconsin Alumni Research Foundation Influenza viruses with mutant pb2 gene segment as live attenuated vaccines
AU2013240248B2 (en) 2012-03-26 2018-12-20 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Delivery of packaged RNA to mammalian cells
AU2014365777B2 (en) * 2013-12-16 2020-06-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cancer immunotherapy by delivering class II MHC antigens using a VLP-replicon
US10053671B2 (en) 2014-06-20 2018-08-21 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to additional genes in influenza viruses
WO2016037165A1 (en) 2014-09-07 2016-03-10 Selecta Biosciences, Inc. Methods and compositions for attenuating exon skipping anti-viral transfer vector immune responses
FR3036118B1 (fr) * 2015-05-15 2019-07-12 Flash Therapeutics Particule retrovirale comportant au moins deux arn non viraux encapsides.
ES2856901T3 (es) * 2015-05-15 2021-09-28 Flash Therapeutics Partícula retrovírica que contiene al menos dos ARN no víricos encapsidados diferentes
ES3046235T3 (en) 2015-06-19 2025-12-01 Lgc Clinical Diagnostics Inc Sindbis control virus
EP3341018A1 (en) * 2015-08-28 2018-07-04 Wisconsin Alumni Research Foundation Generation of infectious influenza viruses from virus-like particles
WO2017083356A1 (en) 2015-11-09 2017-05-18 Immune Design Corp. A retroviral vector for the administration and expression of replicon rna expressing heterologous nucleic acids
US11197925B2 (en) 2016-02-19 2021-12-14 Wisconsin Alumni Research Foundation (Warf) Influenza B virus replication for vaccine development
BR112019013862A2 (pt) 2017-01-07 2020-04-14 Selecta Biosciences Inc dosagem padrão de imunossupressores acoplados a nanocarreadores sintéticos
WO2018176103A1 (en) 2017-03-30 2018-10-04 The University Of Queensland "chimeric molecules and uses thereof"
US12076369B2 (en) 2017-09-01 2024-09-03 The Frances Crick Institute Limited Immunoregulatory molecules and uses therefor
AU2018347583A1 (en) 2017-10-13 2020-05-21 Selecta Biosciences, Inc. Methods and compositions for attenuating anti-viral transfer vector IgM responses
US11197926B2 (en) 2017-10-25 2021-12-14 Wisconsin Alumni Research Foundation (Warf) Recombinant influenza viruses with stabilized HA for replication in eggs
MX2020006435A (es) 2017-12-19 2021-02-09 Akouos Inc Administracion de anticuerpos terapeuticos mediada por vector de virus adenoasociado (aav) en el oido interno.
JP2021515037A (ja) 2018-02-26 2021-06-17 アントルクス，インコーポレーテッド 寛容原性リポソーム及びその使用方法
WO2020013317A1 (ja) * 2018-07-13 2020-01-16 国立大学法人京都大学 ウイルス様粒子及びその使用
WO2020033527A2 (en) 2018-08-07 2020-02-13 Wisconsin Alumni Research Foundation (Warf) Recombinant biologically contained filovirus vaccine
WO2020041311A1 (en) 2018-08-20 2020-02-27 Wisconsin Alumni Research Foundation Vectors for eliciting immune responses to non-dominant epitopes in the hemagglutinin (ha) protein
EP3921413A1 (en) 2019-02-08 2021-12-15 Wisconsin Alumni Research Foundation (WARF) Humanized cell line
EP3962537A1 (en) 2019-04-28 2022-03-09 Selecta Biosciences, Inc. Methods for treatment of subjects with preexisting immunity to viral transfer vectors
WO2020223699A1 (en) 2019-05-01 2020-11-05 Wisconsin Alumni Research Foundation (Warf) Improved influenza virus replication for vaccine development
EP3976802A1 (en) 2019-05-28 2022-04-06 Selecta Biosciences, Inc. Methods and compositions for attenuated anti-viral transfer vector immune response
US20220259617A1 (en) 2019-06-13 2022-08-18 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
US20220396806A1 (en) 2019-07-26 2022-12-15 Akouos, Inc. Methods of treating hearing loss using a secreted target protein
WO2021041624A2 (en) 2019-08-27 2021-03-04 Yoshihiro Kawaoka Recombinant influenza viruses with stabilized ha for replication in eggs
WO2021150874A1 (en) 2020-01-24 2021-07-29 Wisconsin Alumni Research Foundation (Warf) Recombinant influenza viruses with stabilized na
US12290562B2 (en) 2020-03-25 2025-05-06 Wisconsin Alumni Research Foundation (Warf) Recombinant multivalent influenza viruses
US20230212606A1 (en) 2020-05-13 2023-07-06 Akouos, Inc. Compositions and methods for treating kcnq4-associated hearing loss
BR112022022906A2 (pt) 2020-05-13 2023-01-17 Akouos Inc Composições e métodos para tratar perda auditiva associada a slc26a4
BR112023001272A2 (pt) 2020-07-24 2023-04-04 Massachusetts Gen Hospital Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células
CA3197936A1 (en) 2020-12-01 2022-06-09 Akouos, Inc. Anti-vegf antibody constructs and related methods for treating vestibular schwannoma associated symptoms
MX2023007800A (es) 2020-12-29 2023-07-11 Akouos Inc Composiciones y metodos para tratar la perdida de audicion y/o la perdida de vision asociada a clrn1.
WO2022261355A1 (en) 2021-06-09 2022-12-15 Chimeron Bio Corporation Self-amplifying rna-based vlp vaccines
WO2022261343A1 (en) 2021-06-09 2022-12-15 Chimeron Bio Corporation Peptide vlp-based vaccines
MX2024003778A (es) 2021-09-30 2024-04-10 Akouos Inc Composiciones y metodos para el tratamiento de la perdida de audicion asociada a kcnq4.
WO2023064367A1 (en) 2021-10-12 2023-04-20 Selecta Biosciences, Inc. Methods and compositions for attenuating anti-viral transfer vector igm responses
AR128422A1 (es) 2022-02-02 2024-05-08 Akouos Inc Construcciones de anticuerpos anti-vegf y métodos relacionados para el tratamiento de los síntomas asociados al schwannoma vestibular
WO2023172624A1 (en) 2022-03-09 2023-09-14 Selecta Biosciences, Inc. Immunosuppressants in combination with anti-igm agents and related dosing
TW202408545A (zh) 2022-07-08 2024-03-01 美國貝勒醫學院 整合的壓力反應抑制劑及其使用方法
AU2024266138A1 (en) 2023-05-03 2025-11-13 Manifold Biotechnologies, Inc. Methods and compositions for high-throughput protein delivery, screening, and detection

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080118956A1 (en) * 2003-12-02 2008-05-22 Jean-Christophe Pages Viral Particles Containing An Alphavirus-Derived Vector And Method For Preparing Said Viral Particle
WO2008115199A2 (en) * 2006-08-18 2008-09-25 The University Of North Carolina At Chapel Hill Chimeric virus vaccines

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1004083A (en) 1911-01-09 1911-09-26 Henry Ritter Method of making sash-bars and die for forming same.
US5374426A (en) 1986-09-03 1994-12-20 University Of Saskatchewan Rotavirus nucleocapsid protein VP6 in vaccine compositions
AU9052091A (en) 1990-12-20 1992-07-22 Smithkline Beecham Biologicals (Sa) Vaccines based on hepatitis b surface antigen
US5631237A (en) 1992-12-22 1997-05-20 Dzau; Victor J. Method for producing in vivo delivery of therapeutic agents via liposomes
EP0817854A2 (en) 1995-03-31 1998-01-14 Wolf, Hans, Prof. Dr. Antigen presentation system based on retrovirus-like particles
US5792462A (en) 1995-05-23 1998-08-11 University Of North Carolina At Chapel Hill Alphavirus RNA replicon systems
GB9621091D0 (en) 1996-10-09 1996-11-27 Fondation Pour Le Perfectionem Attenuated microorganisms strains and their uses
US6099847A (en) 1997-05-15 2000-08-08 The United States Of America As Represented By The Department Of Health And Human Services Chimeric Gag pseudovirions
US6555342B1 (en) 1998-06-03 2003-04-29 Uab Research Foundation Fusion protein delivery system and uses thereof
US6902886B1 (en) 1998-11-06 2005-06-07 The Research Foundation Of State University Of New York Genetic assay for protein nuclear transport
US20020052040A1 (en) 1999-06-30 2002-05-02 Nicholas Hunt Virus like particles, their preparation and their use preferably in pharmaceutical screening and functional genomics
US20020177551A1 (en) 2000-05-31 2002-11-28 Terman David S. Compositions and methods for treatment of neoplastic disease
US6783939B2 (en) 2000-07-07 2004-08-31 Alphavax, Inc. Alphavirus vectors and virosomes with modified HIV genes for use in vaccines
ATE437222T1 (de) 2001-09-06 2009-08-15 Alphavax Inc Alphavirus replikon-vektorsysteme
CA2492826C (en) 2001-09-14 2016-12-13 Cytos Biotechnology Ag Encapsulation of unmethylated cpg-containing oligonucleotides into virus-like particles: method of preparation and use
US20080115199A1 (en) * 2001-12-06 2008-05-15 Albert Young Scheme for device and user authentication with key distribution in a wireless network
US8574590B2 (en) 2003-07-30 2013-11-05 Integral Molecular, Inc. Lipoparticles comprising proteins, methods of making, and using the same
WO2005115444A2 (en) 2004-04-14 2005-12-08 Avirid, Inc. Compositions with modified nucleases targeted to viral nucleic acids and methods of use for prevention and treatment of viral diseases
GB0426641D0 (en) 2004-12-03 2005-01-05 Bioactive Protein Delivery The Protein delivery system
WO2007130330A2 (en) 2006-05-01 2007-11-15 Technovax, Inc. Polyvalent influenza virus-like particle (vlp) compositions
DE202006010918U1 (de) * 2006-07-14 2006-10-05 Flexi-Bogdahn Technik Gmbh & Co. Kg Leineneinrichtung für eine mechanisch auf- und abrollbare Leine zum Führen von Tieren
US20110250675A1 (en) * 2008-03-26 2011-10-13 Life Technologies Corporaiton Virus-Like Particle Mediated Cellular Delivery
WO2010040023A2 (en) 2008-10-03 2010-04-08 Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Methods and compositions for protein delivery
JP5917402B2 (ja) * 2009-11-03 2016-05-11 タケダ ヴァクシーンズ， インコーポレイテッド キメラＲＳＶ−Ｆポリペプチド、およびレンチウイルスＧａｇまたはアルファレトロウイルスＧａｇに基づくＶＬＰ
AU2013240248B2 (en) 2012-03-26 2018-12-20 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Delivery of packaged RNA to mammalian cells
AU2014365777B2 (en) 2013-12-16 2020-06-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cancer immunotherapy by delivering class II MHC antigens using a VLP-replicon

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080118956A1 (en) * 2003-12-02 2008-05-22 Jean-Christophe Pages Viral Particles Containing An Alphavirus-Derived Vector And Method For Preparing Said Viral Particle
WO2008115199A2 (en) * 2006-08-18 2008-09-25 The University Of North Carolina At Chapel Hill Chimeric virus vaccines

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Jurgens CK et al, "A novel self-replicating chimeric lentivirus-like particle", Journal of Virology, 2012, 86(1):246-261, Epub 19 October 2011 *
Piver E et al, "Mobilization of full-length Semliki Forest virus replicon by retrovirus particles", Journal of Virology, 2006, 80(19):9889-9895 *

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