2011101378 Editorial Note There are only 9 pages of description.
Descriptions Ttraditional Chinese Medicine Perfusate For The Treatment Of Dairy Cow Mastitis And A Preparation Method Thereof Technical Field The invention belongs to the field of veterinary medicines, especially to a traditional Chinese medicine perfusate for the treatment of dairy cow mastitis and a preparation method thereof. Background Art Dairy cow mastitis, one of the commonest cow diseases, includes 80 to 130 types of pathogens, among which streptococcus agalactiae, streptococcus dysgalactiae, staphylococcus aureus, mycoplasma, streptococcus uberis, escherichia coli and the like are mainly representative. Dairy cow mastitis is generally classified into two types: 1. subclinical mastitis, which is characterized in that, the body defense mechanism at the early stage of mastitis onset struggles roughly with pathogens, no inflammation and thermal pain occurs outside mammary glands, abnormal change of milk quality is not observed from outside, however, it can still be seen according to laboratory analysis that the number of somatic cells in milk surpasses 0.5 million per mL; 2, clinical mastitis, which is characterized in that, the abnormalities of both mammary glands and milk are observed, and flocculent milk dregs, coagulum and water-like milk serum are present in milk. The udder site infected by acute mastitis has the following symptoms: sudden swelling, reddish skin, fever and hardening. The dairy cow suffering from the acute mastitis has the following symptoms: body temperature rise (to over 41 'C sometimes), quickened pulse, polypnea, mental depression, inappetence, extreme emaciation along with the progression of disease, lying all the time. In case of severe mastitis, the dairy cow has the following symptoms: enophthalmos, stinky excrement and physical exhaustion, and finally dies of septicemia. Data statistics show that, about 50% of 1.1 million lactating cows in dairy farming-prosperous U.S.A at present suffers from the acute mastitis, and according to the investigations in Beijing, Shanghai and other cities in our country, the incidence rate of the acute mastitis is about 60%, the loss caused by dairy cow mastitis in our country is economically equal to 1526.25 Yuan of every diseased cow every year, hence, it can be easily found that the dairy cow mastitis has become one of important reasons for economic loss of cow production. Currently, what is used in general for the treatment of the dairy cow mastitis is antibiotics, which, although having particular curative effect, can result in the severe problems of medicine residue and polluted animal-derived food and do large stimulation harm to cultivated animals so as to cause strong stress response. Summary of Invention One objective of the invention is to provide a traditional Chinese medicine perfusate for the treatment of dairy cow mastitis, which has the advantages of fast effect, no toxic and side effect, Descriptions no medicine residue, convenient usage, saved cultivation cost, simplified operation and high bioavailability Another objective of the invention is to provide a preparation method of the traditional Chinese medicine perfusate for the treatment of dairy cow mastitis. The invention is implemented by the following technical proposal: A traditional Chinese medicine perfusate for the treatment of dairy cow mastitis contains the following ingredients in parts by weight: fructus mume extract 1 to 10 parts; spine date seed extract I to 10 parts; heartleaf houttuynia herb extract 1 to 10 parts; absolute ethyl alcohol 5 to 30 parts; purified water 10 to 50 parts. A preparation method of the traditional Chinese medicine perfusate for the treatment of dairy cow mastitis comprises the following steps of: (1) weighing and crushing spine data seed, then soak-degreasing the crushed spine data seed in petroleum ether which is 6 to 8 times as heavy as the spine data seed, continuously soaking and extracting, 3 to 4 times with each for 3 to 5 hours, the resultant spine data seed dregs in 60 to 80% ethanol which is 6 to 10 times as heavy as the spine data seed dregs, collecting filtrate, recovering the ethanol, and concentrating and drying ointment to obtain the spine date seed extract for future use; (2) weighing and crushing fructus mume, then soak-degreasing the crushed fructus mume in petroleum ether which is 6 to 8 times as heavy as the fructus mume, continuously soaking and extracting, I to 4 times with each for 1 to 4 hours, the resultant fructus mume dregs in n-butanol which is I to 4 times as heavy as the fructus mume dregs, collecting filtrate, recovering the n-butanol, and concentrating and drying ointment to obtain the fructus mume extract for future use; (3) weighing and crushing heartleaf houttuynia herb, then soak-degreasing the crushed heartleaf houttuynia herb in petroleum ether which is 6 to 8 times as heavy as the heartleaf houttuynia herb, continuously soaking and extracting, 3 to 4 times with each for 3 to 5 hours, the resultant heartleaf houttuynia herb dregs in 60 to 80% ethanol which is 6 to 10 times as heavy as the heartleaf houttuynia herb dregs, collecting filtrate, recovering the ethanol, and concentrating and drying ointment to heartleaf houttuynia herb extract for future use; (4) respectively weighing, in accordance with the weight proportion, and then crushing the spine date seed extract, the fructus mume extract and the heartleaf houttuynia herb extract, sieving Descriptions the crushed extracts via a 80- to 100-mesh sieve, uniformly mixing the crushed extracts, dissolving the mixture in absolute ethyl alcohol in accordance with the weight proportion, then adding purified water in accordance with the weight proportion to the absolute ethyl alcohol for dilution, and finally, steam-sterilizing the absolute ethyl alcohol to obtain the finished product of the traditional Chinese medicine perfusate for the treatment of dairy cow mastitis. The invention has the advantages that: 1. The traditional Chinese medicine perfusate for the treatment of dairy cow mastitis adopts the fructus mume extrac, the spine date seed extract and the heartleaf houttuynia herb extract as traditional Chinese medicine effective ingredients, and has the outstanding effects of cleaning away heat and toxic materials, resisting bacteria and eliminating inflammation and soothing nerves and killing pains. And simultaneously, as purely natural traditional Chinese medicine extract, the perfusate is free from any toxic and harmful substances, causes no toxic and side effect to animal body and has no influence on the healthy of human body. 2. The traditional Chinese medicine perfusate for the treatment of dairy cow mastitis is characterized by incorporating perfusion technology into the medicinal preparation, and when the traditional way of mixed administration cannot take effect, the traditional Chinese medicine perfusate can lead administration treatment to be more efficient, thereby saving plenty of manpower and material resources, causing the effective ingredients in the medicine to be dissolved fast and uniformly, facilitating the absorption of livestock, enhancing bioavailability and dramatically improving the clinical effect of the medicine. 3. The invention has the advantages of fast effect, no toxic and side effect, no medicine residue, convenient usage, saved cultivation cost, simplified operation and high bioavailability, and is a traditional Chinese medicine perfusate for the treatment of dairy cow mastitis conforming to the requirement of safe veterinary medicines and guaranteeing safe animal-derived food. Detailed Description of Embodiments Detailed description is further made below to the invention with reference to the embodiments below. It shall be noted that, the embodiments described hereinafter, illustrative not limiting, shall not be considered as the limitation to the scope of protection of the invention. Embodiment 1 A traditional Chinese medicine perfusate for the treatment of dairy cow mastitis contains the following ingredients in parts by weight: lkg of fructus mume extract; 1kg of spine date seed extract; lkg of heartleaf houttuynia herb extract; 5kg of absolute ethyl alcohol and 10kg of purified water. A preparation method of the traditional Chinese medicine perfusate for the treatment of dairy Descriptions cow mastitis comprises the following steps of: (1) weighing and crushing spine data seed, then soak-degreasing the crushed spine data seed in petroleum ether which is 6 times as heavy as the spine data seed, continuously soaking and extracting, 3 times with each for 3 hours, the resultant spine data seed dregs in 60% ethanol which is 6 times as heavy as the spine data seed dregs, collecting filtrate, recovering the ethanol, and concentrating and drying ointment to obtain the spine date seed extract for future use; (2) weighing and crushing fructus mume, then soak-degreasing the crushed fructus mume in petroleum ether which is 6 times as heavy as the fructus mume, continuously soaking and extracting, once for 1 hour, the resultant fructus mume dregs in n-butanol which is twice as heavy as the fructus mume dregs, collecting filtrate, recovering the n-butanol, and concentrating and drying ointment to obtain the fructus mume extract for future use; (3) weighing and crushing heartleaf houttuynia herb, then soak-degreasing the crushed heartleaf houttuynia herb in petroleum ether which is 6 times as heavy as the heartleaf houttuynia herb, continuously soaking and extracting, 3 times with each for 3 hours, the resultant heartleaf houttuynia herb dregs in 60% ethanol which is 6 times as heavy as the heartleaf houttuynia herb dregs, collecting filtrate, recovering the ethanol, and concentrating and drying ointment to heartleaf houttuynia herb extract for future use; (4) respectively weighing, in accordance with the weight proportion, and then crushing the spine date seed extract, the fructus mume extract and the heartleaf houttuynia herb extract, sieving the crushed extracts via a 80-mesh sieve, uniformly mixing the crushed extracts, dissolving the mixture in absolute ethyl alcohol in accordance with the weight proportion, then adding purified water in accordance with the weight proportion to the absolute ethyl alcohol for dilution, and finally, steam-sterilizing the absolute ethyl alcohol to obtain the finished product of the traditional Chinese medicine perfusate for the treatment of dairy cow mastitis. Embodiment 2 A traditional Chinese medicine perfusate for the treatment of dairy cow mastitis contains the following ingredients in parts by weight: 10kg of fructus mume extract; 10kg of spine date seed extract; 10kg of heartleaf houttuynia herb extract; 30kg of absolute ethyl alcohol and 50kg of purified water. A preparation method of the traditional Chinese medicine perfusate for the treatment of dairy cow mastitis comprises the following steps of: (1) weighing and crushing spine data seed, then soak-degreasing the crushed spine data seed in petroleum ether which is 8 times as heavy as the spine data seed, continuously soaking and extracting, 4 times with each for 5 hours, the resultant spine data seed dregs in 80% ethanol which Descriptions is 10 times as heavy as the spine data seed dregs, collecting filtrate, recovering the ethanol, and concentrating and drying ointment to obtain the spine date seed extract for future use; (2) weighing and crushing fructus mume, then soak-degreasing the crushed fructus mume in petroleum ether which is 8 times as heavy as the fructus mume, continuously soaking and extracting, four times with each for 4 hours, the resultant fructus mume dregs in n-butanol which is four times as heavy as the fructus mume dregs, collecting filtrate, recovering the n-butanol, and concentrating and drying ointment to obtain the fructus mume extract for future use; (3) weighing and crushing heartleaf houttuynia herb, then soak-degreasing the crushed heartleaf houttuynia herb in petroleum ether which is 8 times as heavy as the heartleaf houttuynia herb, continuously soaking and extracting, 4 times with each for 5 hours, the resultant heartleaf houttuynia herb dregs in 80% ethanol which is 10 times as heavy as the heartleaf houttuynia herb dregs, collecting filtrate, recovering the ethanol, and concentrating and drying ointment to heartleaf houttuynia herb extract for future use; (4) respectively weighing, in accordance with the weight proportion, and then crushing the spine date seed extract, the fructus mume extract and the heartleaf houttuynia herb extract, sieving the crushed extracts via a 100-mesh sieve, uniformly mixing the crushed extracts, dissolving the mixture in absolute ethyl alcohol in accordance with the weight proportion, then adding purified water in accordance with the weight proportion to the absolute ethyl alcohol for dilution, and finally, steam-sterilizing the absolute ethyl alcohol to obtain the finished product of the traditional Chinese medicine perfusate for the treatment of dairy cow mastitis. Embodiment 3 A traditional Chinese medicine perfusate for the treatment of dairy cow mastitis contains the following ingredients in parts by weight: 5kg of fructus mume extract; 5kg of spine date seed extract; 5kg of heartleaf houttuynia herb extract; 20kg of absolute ethyl alcohol and 25kg of purified water. The preparation method in this embodiment is the same as that in the embodiment 1. Embodiment 4 A traditional Chinese medicine perfusate for the treatment of dairy cow mastitis contains the following ingredients in parts by weight: 3kg of fructus mume extract; 6kg of spine date seed extract; 6kg of heartleaf houttuynia herb extract; 25kg of absolute ethyl alcohol and 30kg of purified water. The preparation method in this embodiment is the same as that in the embodiment 2. Clinical application experiment In order to verify the curative effect of the traditional Chinese medicine perfusate for the Descriptions treatment of dairy cow mastitis on natural cases of dairy cow mastitis, we carry out the experiment for the treatment of dairy cow mastitis in cow cultivation base, Wuqing District, Tianjin. In this experiment, administration dose is given to every group of perfusate differently, and the curative effect of the perfusate is compared with that of conventional medicine for the treatment of dairy cow mastitis, in order to evaluate the curative effect and the optimal dose of the perfusate in relation to dairy cow mastitis. Materials and methods 1.1 Experimental animal 20 dairy cows suffering from mastitis, with every four of them being grouped, are chosen. The cows are bred as normal breeding management procedures. 1.2 Experimental medicine The perfusate for this experiment is prepared by the company. RUZHONGKANG injection: 20ml per bottle, Heilongjiang Beian Feilong Veterinary Medicine Factory, production batch number 081004. 1.3 Experimental method Mastitis cases are mainly determined by milkers who inspect the property of udder and milk in conjunction with BMT method, and are then subjected to randomly-grouped treatment by veterinarian. 1.4 Diagnostic standards of mastitis Clinical mastitis is featured by milk degeneration, swells of udder tissue to different extents, damp heat and pains, clinical mastitis can be diagnosed in the cow having the following symptoms or a part of symptoms: increased udder volume, fever and hard udder tissue; reddish udder skin and palpation-derived pain; different sizes of udder sites and enlargement of supramammary lymph nodes; and the abnormalities such as milk discoloration, viscous milk, the appearance of floccules, coagulum, blood clot and the like. 1.5 Grouped administration The cases are respectively and randomly put into the experimental medicine groups of the invention, including Group A, Group B, Group C and Group D based on different doses thereof as well as a RUZHONGKANG injection control group as below: A: 10ml of perfusate is injected through the affected teats to mammary glands in the direction of cistern once a day, and the course of treatment lasts 5 days. B: 20ml of perfusate is injected through the affected teats to mammary glands in the direction of cistern once a day, and the course of treatment lasts 5 days. C: 30m] of perfusate is injected through the affected teats to mammary glands in the direction Descriptions of cistern once a day, and the course of treatment lasts 5 days. D: 40ml of perfusate is injected through the affected teats to mammary glands in the direction of cistern once a day, and the course of treatment lasts 5 days. RUZHONGKANG injection control group: 150m] of perfusate is injected through the affected teats to mammary glands in the direction of cistern once a day, and the course of treatment lasts 5 days. The medicine for each group is injected to teat mouths via a cow lactation promoting needle and then the teat mouths are all gently pinched, and udder is gently massaged to push the medicine toward the upper parts of the mammary glands. 1.6 Method for determining the curative effect Cured: clinical symptoms disappear, no abnormality of milk is directly observed, milk production substantially returns to normal, and the detection result of the BMT method is lower than '++'. Effective: clinical symptoms are mitigated or disappear, a small number of floccules are present in milk, but no abnormality of milk color is observed, milk production fails to return to normal, however, the detection result of the BMT method is still higher than '++'. Ineffective: various clinical symptoms in determination standard are not mitigated or even exacerbated. 1.7 Statistical analysis According to chi-square test, the curative ratio and the total effective rate of the Group A is significantly lower than those of other groups (P<0.05), and the differences between the other groups are insignificant (P<0.05). 2 Experimental result 2.1 Effects of the perfusate treatment on productivity The results are shown in Table 1. Clinical symptoms of the cows suffering from mastitis are mitigated after different doses of perfusate are administered, milk production returns to normal and mastitis symptoms are relieved to some extent. However, the curative effect in the Group A is not obvious, a small amount of blood still appears in milk and milk viscosity is raised; mastitis symptoms of cows in the RUZHONGKANG control group are relieved, while milk production decreases. Table 1 Curative Effect Observation Medic A B C D RUZHO ine NGKANG Control Group Descriptions Quant 4 4 4 4 4 ity__ _ _ _ _ _ Medic mastitis mastitis mastitis mastitis mastitis al History Treat RUZHO RUZHO RUZHO RUZHO RUZHO ment NGKANG NGKANG NGKANG NGKANG NGKANG History Curati effective cured cured cured effective ve Effect Post-T milk milk milk milk milk reatment production production production production production Production returns to returns to returns to returns to returns to Index normal normal normal normal normal 2.2 Effects of the treatment on relieving mastitis For the results, see Table 2. Table 2 Medicine Patho Uncur Average Treatment Curative genic ed Udder Times Ratio (%) Udder Site Site A 12 4 5 75% B 16 2 4 87.5 C 16 0 2 100% D 10 0 3 100% RUZHONGKAN 16 1 3 93.75 G Control Group 3. Result analysis According to the analyses in the aspects of clinical symptoms, milk production treatment, relieving mastitis symptoms and curative ratio, the curative effects in the Groups C and D are significantly higher than those in the other treatment groups. Certain curative effect can be observed in the control group, nonetheless, the recovery cycle for the treated cows is long and the economic loss is large. 4. Conclusion 30ml of the perfusate of the invention is administered, once a day, continuously for five days Descriptions in order for the treatment of clinical mastitis, thereby achieving the best curative effect for dairy cow mastitis.