AU2010339544B2 - Production of hemagglutinin-neuraminidase protein in microalgae - Google Patents

Production of hemagglutinin-neuraminidase protein in microalgae Download PDF

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Publication number
AU2010339544B2
AU2010339544B2 AU2010339544A AU2010339544A AU2010339544B2 AU 2010339544 B2 AU2010339544 B2 AU 2010339544B2 AU 2010339544 A AU2010339544 A AU 2010339544A AU 2010339544 A AU2010339544 A AU 2010339544A AU 2010339544 B2 AU2010339544 B2 AU 2010339544B2
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Australia
Prior art keywords
polypeptide
heterologous
protein
host cell
microalgal
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AU2010339544A1 (en
Inventor
Kirk E. Apt
Anne-Cecile V. Bayne
Xuan GUO
James Casey Lippmeier
Joyce A. Pritchard
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Sanofi Vaccine Technologies SAS
Merial LLC
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Sanofi Vaccine Technologies SAS
Merial Ltd
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Assigned to SANOFI VACCINE TECHNOLOGIES, S.A.S., MERIAL LIMITED reassignment SANOFI VACCINE TECHNOLOGIES, S.A.S. Request for Assignment Assignors: DSM IP ASSETS B.V., MERIAL LIMITED
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18111—Avulavirus, e.g. Newcastle disease virus
    • C12N2760/18151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
    • C12N2760/18651—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2010339544A 2009-12-28 2010-12-28 Production of hemagglutinin-neuraminidase protein in microalgae Active AU2010339544B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29046909P 2009-12-28 2009-12-28
US61/290,469 2009-12-28
PCT/US2010/062274 WO2011082189A1 (en) 2009-12-28 2010-12-28 Production of hemagglutinin-neuraminidase protein in microalgae

Publications (2)

Publication Number Publication Date
AU2010339544A1 AU2010339544A1 (en) 2012-08-09
AU2010339544B2 true AU2010339544B2 (en) 2015-11-12

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Application Number Title Priority Date Filing Date
AU2010339544A Active AU2010339544B2 (en) 2009-12-28 2010-12-28 Production of hemagglutinin-neuraminidase protein in microalgae

Country Status (9)

Country Link
US (1) US9012197B2 (enExample)
EP (1) EP2519636B8 (enExample)
JP (1) JP5838473B2 (enExample)
CN (1) CN103068984B (enExample)
AU (1) AU2010339544B2 (enExample)
CA (1) CA2785867C (enExample)
EA (1) EA030095B1 (enExample)
IN (1) IN2012DN06278A (enExample)
WO (1) WO2011082189A1 (enExample)

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TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
CN102648207A (zh) 2009-03-16 2012-08-22 马太克生物科学公司 在网粘菌门微生物中产生蛋白质
CA2785971C (en) * 2009-12-28 2019-02-12 Dsm Ip Assets B.V. Production of heterologous polypeptides in microalgae, microalgal extracellular bodies, compositions, and methods of making and uses thereof
EP3213766A1 (en) * 2009-12-28 2017-09-06 Merial Ltd. Recombinant ndv antigen and uses thereof
CA2993690C (en) 2010-01-19 2022-03-22 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
MX342216B (es) * 2011-07-21 2016-09-20 Dsm Ip Assets Bv Composiciones de acidos grasos.
CN103525785B (zh) * 2013-09-24 2015-03-25 西北大学 一种分离纯化神经氨酸酶（禽流感病毒）的方法
EP3265567B1 (en) 2015-03-02 2020-07-29 Conagen Inc. Regulatory elements from labyrinthulomycetes microorganisms
EP3535385A4 (en) 2016-11-01 2020-06-17 Conagen Inc. EXPRESSION OF MODIFIED GLYCOPROTEINS AND GLYCOPEPTIDES
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
EP3788164A4 (en) * 2018-05-01 2022-01-26 Conagen Inc. RECOMBINANT ORGANISMS AND METHODS FOR PRODUCTION OF HIGH GLYCAN OCCUPANCY GLYCOMOLECULES
AR127808A1 (es) 2021-11-30 2024-02-28 Sanofi Pasteur Inc Vacunas contra el metapneumovirus humano

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US20070128222A1 (en) * 2003-06-05 2007-06-07 Wyeth Holdings Corporation Fusogenic, self-propagating blebs as immunogenic compositions

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US4752473A (en) 1984-10-12 1988-06-21 The Regents Of The University Of California Expression of glycosylated human influenza hemagglutinin proteins
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5762939A (en) 1993-09-13 1998-06-09 Mg-Pmc, Llc Method for producing influenza hemagglutinin multivalent vaccines using baculovirus
WO1997039106A1 (en) * 1996-04-12 1997-10-23 Martek Biosciences Corporation Methods and tools for transformation of eukaryotic algae
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US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
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KR20060035596A (ko) 2003-05-05 2006-04-26 보이스 톰슨 인스터튜트 포 플랜트 리서치 외래유전자도입 식물로부터 유래된 면역보호성 조성물을제조하기 위한 벡터와 세포
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EP3213766A1 (en) 2009-12-28 2017-09-06 Merial Ltd. Recombinant ndv antigen and uses thereof
CA2785971C (en) 2009-12-28 2019-02-12 Dsm Ip Assets B.V. Production of heterologous polypeptides in microalgae, microalgal extracellular bodies, compositions, and methods of making and uses thereof

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Also Published As

Publication number Publication date
IN2012DN06278A (enExample) 2015-09-25
WO2011082189A1 (en) 2011-07-07
JP5838473B2 (ja) 2016-01-06
US20110195480A1 (en) 2011-08-11
EA201290565A1 (ru) 2013-02-28
EP2519636A4 (en) 2013-07-03
EP2519636B1 (en) 2017-03-01
CN103068984A (zh) 2013-04-24
US9012197B2 (en) 2015-04-21
CA2785867A1 (en) 2011-07-07
CA2785867C (en) 2018-03-27
EA030095B1 (ru) 2018-06-29
EP2519636B8 (en) 2017-08-09
CN103068984B (zh) 2017-12-01
JP2013515502A (ja) 2013-05-09
EP2519636A1 (en) 2012-11-07
AU2010339544A1 (en) 2012-08-09

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