CN103068984B - 微藻中血凝素‑神经氨酸酶蛋白的生产 - Google Patents

微藻中血凝素‑神经氨酸酶蛋白的生产 Download PDF

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Publication number
CN103068984B
CN103068984B CN201080064860.1A CN201080064860A CN103068984B CN 103068984 B CN103068984 B CN 103068984B CN 201080064860 A CN201080064860 A CN 201080064860A CN 103068984 B CN103068984 B CN 103068984B
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China
Prior art keywords
polypeptide
heterologous
host cell
protein
schizochytrium
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Expired - Fee Related
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CN201080064860.1A
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English (en)
Chinese (zh)
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CN103068984A (zh
Inventor
K.E.埃普特
A-C.V.贝恩
J.C.利普梅尔
X.郭
J.A.普利特查德
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Merial Ltd
Dsmip Asset Co
DSM IP Assets BV
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Merial Ltd
Dsmip Asset Co
DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18111—Avulavirus, e.g. Newcastle disease virus
    • C12N2760/18151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
    • C12N2760/18651—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201080064860.1A 2009-12-28 2010-12-28 微藻中血凝素‑神经氨酸酶蛋白的生产 Expired - Fee Related CN103068984B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29046909P 2009-12-28 2009-12-28
US61/290,469 2009-12-28
PCT/US2010/062274 WO2011082189A1 (en) 2009-12-28 2010-12-28 Production of hemagglutinin-neuraminidase protein in microalgae

Publications (2)

Publication Number Publication Date
CN103068984A CN103068984A (zh) 2013-04-24
CN103068984B true CN103068984B (zh) 2017-12-01

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CN201080064860.1A Expired - Fee Related CN103068984B (zh) 2009-12-28 2010-12-28 微藻中血凝素‑神经氨酸酶蛋白的生产

Country Status (9)

Country Link
US (1) US9012197B2 (enExample)
EP (1) EP2519636B8 (enExample)
JP (1) JP5838473B2 (enExample)
CN (1) CN103068984B (enExample)
AU (1) AU2010339544B2 (enExample)
CA (1) CA2785867C (enExample)
EA (1) EA030095B1 (enExample)
IN (1) IN2012DN06278A (enExample)
WO (1) WO2011082189A1 (enExample)

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TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
TWI548648B (zh) 2009-03-16 2016-09-11 Ｄｓｍ智慧財產有限公司 於網黏菌門微生物中生產蛋白質之技術
AU2010343095B2 (en) * 2009-12-28 2017-01-05 Sanofi Vaccine Technologies, S.A.S. Production of heterologous polypeptides in microalgae, microalgal extracellular bodies, compositions, and methods of making and uses thereof
MX351643B (es) * 2009-12-28 2017-10-23 Merial Ltd Antigeno ndv recombinante y usos del mismo.
NZ601757A (en) 2010-01-19 2015-01-30 Dsm Ip Assets Bv Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
EP2952183A1 (en) 2011-07-21 2015-12-09 DSM IP Assets B.V. Fatty acid compositions
CN103525785B (zh) * 2013-09-24 2015-03-25 西北大学 一种分离纯化神经氨酸酶（禽流感病毒）的方法
WO2016140925A1 (en) 2015-03-02 2016-09-09 Synthetic Genomics, Inc. Regulatory elements from labyrinthulomycetes microorganisms
WO2018085273A1 (en) 2016-11-01 2018-05-11 Synthetic Genomics, Inc. Expression of modified glycoproteins and glycopeptides
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
CN112074608A (zh) * 2018-05-01 2020-12-11 科纳根公司 用于产生具有高聚糖占有率的糖分子的重组生物体和方法
CA3239417A1 (en) 2021-11-30 2023-06-08 Yvonne CHAN Human metapneumovirus vaccines

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US4659669A (en) 1981-03-02 1987-04-21 Regents Of The Univ. Of California Microbial expression of human influenza hemagglutinin proteins
US4752473A (en) 1984-10-12 1988-06-21 The Regents Of The University Of California Expression of glycosylated human influenza hemagglutinin proteins
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5762939A (en) 1993-09-13 1998-06-09 Mg-Pmc, Llc Method for producing influenza hemagglutinin multivalent vaccines using baculovirus
US6027900A (en) * 1996-04-12 2000-02-22 Carnegie Institution Of Washington Methods and tools for transformation of eukaryotic algae
WO1999002695A2 (en) 1997-07-08 1999-01-21 Stichting Instituut Voor Dierhouderij En Diergezondheid Epitopes and active sites of paramyxoviridae proteins and uses thereof
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US20060257399A1 (en) 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
US20060029604A1 (en) 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US7029872B2 (en) 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
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TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
WO2004098533A2 (en) 2003-05-05 2004-11-18 Boyce Thompson Institute For Plant Research Vectors and cells for preparing immunoprotective compositions derived from transgenic plants
WO2005016961A1 (en) * 2003-06-05 2005-02-24 Wyeth Holdings Corporation Immunogenic compositions comprising venezuelan equine encephalitis virus replicon vectors and paramyxovirus protein antigens
AU2005248375B2 (en) 2004-05-25 2010-12-16 Medimmune, Llc Influenza hemagglutinin and neuraminidase varians
US8936930B2 (en) * 2004-08-05 2015-01-20 Ben-Gurion University Of The Negev Research And Development Authority Red microalgae expressing exogenous polypeptides and methods of generating and utilizing same
JP4796787B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
JP4796786B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
TW200813222A (en) 2006-03-15 2008-03-16 Martek Biosciences Corp Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
EP2090648A1 (en) * 2008-02-12 2009-08-19 Institut Francais de Recherche pour l'Exploitation de la Mere(Ifremer) Production of glycosylated polypeptides in microalgae
TWI548648B (zh) 2009-03-16 2016-09-11 Ｄｓｍ智慧財產有限公司 於網黏菌門微生物中生產蛋白質之技術
MX351643B (es) 2009-12-28 2017-10-23 Merial Ltd Antigeno ndv recombinante y usos del mismo.
AU2010343095B2 (en) 2009-12-28 2017-01-05 Sanofi Vaccine Technologies, S.A.S. Production of heterologous polypeptides in microalgae, microalgal extracellular bodies, compositions, and methods of making and uses thereof

Also Published As

Publication number Publication date
JP2013515502A (ja) 2013-05-09
IN2012DN06278A (enExample) 2015-09-25
CN103068984A (zh) 2013-04-24
WO2011082189A1 (en) 2011-07-07
EA201290565A1 (ru) 2013-02-28
EP2519636B1 (en) 2017-03-01
US20110195480A1 (en) 2011-08-11
US9012197B2 (en) 2015-04-21
EA030095B1 (ru) 2018-06-29
JP5838473B2 (ja) 2016-01-06
EP2519636B8 (en) 2017-08-09
CA2785867C (en) 2018-03-27
EP2519636A1 (en) 2012-11-07
EP2519636A4 (en) 2013-07-03
AU2010339544A1 (en) 2012-08-09
CA2785867A1 (en) 2011-07-07
AU2010339544B2 (en) 2015-11-12

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