AU2010222106B2 - Gene for increasing the production of plant biomass and/or seeds and method for use thereof - Google Patents
Gene for increasing the production of plant biomass and/or seeds and method for use thereof Download PDFInfo
- Publication number
- AU2010222106B2 AU2010222106B2 AU2010222106A AU2010222106A AU2010222106B2 AU 2010222106 B2 AU2010222106 B2 AU 2010222106B2 AU 2010222106 A AU2010222106 A AU 2010222106A AU 2010222106 A AU2010222106 A AU 2010222106A AU 2010222106 B2 AU2010222106 B2 AU 2010222106B2
- Authority
- AU
- Australia
- Prior art keywords
- gene
- amino acid
- seq
- protein
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 340
- 238000000034 method Methods 0.000 title claims abstract description 71
- 239000002028 Biomass Substances 0.000 title claims abstract description 60
- 230000001965 increasing effect Effects 0.000 title claims abstract description 33
- 238000004519 manufacturing process Methods 0.000 title claims description 45
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 144
- 108010047313 Protein phosphatase 2C Proteins 0.000 claims abstract description 143
- 102000006831 Protein phosphatase 2C Human genes 0.000 claims abstract description 84
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 claims abstract description 76
- 108010024636 Glutathione Proteins 0.000 claims abstract description 70
- 229960003180 glutathione Drugs 0.000 claims abstract description 70
- 150000001413 amino acids Chemical group 0.000 claims abstract description 68
- 230000014509 gene expression Effects 0.000 claims abstract description 42
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 claims abstract description 35
- 108091035707 Consensus sequence Proteins 0.000 claims abstract description 29
- 241000196324 Embryophyta Species 0.000 claims description 247
- 102000004169 proteins and genes Human genes 0.000 claims description 63
- 230000000694 effects Effects 0.000 claims description 45
- 101100350623 Arabidopsis thaliana At3g05640 gene Proteins 0.000 claims description 44
- 239000002773 nucleotide Substances 0.000 claims description 42
- 125000003729 nucleotide group Chemical group 0.000 claims description 42
- 241000219195 Arabidopsis thaliana Species 0.000 claims description 40
- -1 At3gl6800 Proteins 0.000 claims description 25
- 238000006467 substitution reaction Methods 0.000 claims description 23
- 108091033319 polynucleotide Proteins 0.000 claims description 17
- 102000040430 polynucleotide Human genes 0.000 claims description 17
- 239000002157 polynucleotide Substances 0.000 claims description 17
- 101100082018 Arabidopsis thaliana At4g32950 gene Proteins 0.000 claims description 16
- 101100028362 Arabidopsis thaliana At5g26010 gene Proteins 0.000 claims description 16
- 101100028366 Arabidopsis thaliana At5g36250 gene Proteins 0.000 claims description 16
- 101100350621 Arabidopsis thaliana PPC6-1 gene Proteins 0.000 claims description 16
- 238000003780 insertion Methods 0.000 claims description 14
- 230000037431 insertion Effects 0.000 claims description 14
- 238000012217 deletion Methods 0.000 claims description 12
- 230000037430 deletion Effects 0.000 claims description 12
- 101100082026 Arabidopsis thaliana At5g01700 gene Proteins 0.000 claims description 11
- 101100350626 Arabidopsis thaliana At3g06270 gene Proteins 0.000 claims description 10
- 230000000295 complement effect Effects 0.000 claims description 9
- 230000001747 exhibiting effect Effects 0.000 claims description 9
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims description 5
- 101100055298 Arabidopsis thaliana FBA3 gene Proteins 0.000 claims description 4
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims description 4
- 238000012986 modification Methods 0.000 claims description 4
- 230000004048 modification Effects 0.000 claims description 4
- 229930091371 Fructose Natural products 0.000 claims description 3
- 239000005715 Fructose Substances 0.000 claims description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 3
- 101150098072 20 gene Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 150
- 125000000539 amino acid group Chemical group 0.000 description 79
- 235000001014 amino acid Nutrition 0.000 description 48
- 229940024606 amino acid Drugs 0.000 description 43
- 239000013604 expression vector Substances 0.000 description 40
- 235000018102 proteins Nutrition 0.000 description 39
- 239000012634 fragment Substances 0.000 description 35
- 210000004027 cell Anatomy 0.000 description 28
- 239000013598 vector Substances 0.000 description 26
- 108020004414 DNA Proteins 0.000 description 24
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 24
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 24
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 23
- 239000004474 valine Substances 0.000 description 23
- 240000007594 Oryza sativa Species 0.000 description 22
- 235000007164 Oryza sativa Nutrition 0.000 description 22
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 22
- 229960000310 isoleucine Drugs 0.000 description 22
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 20
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 18
- 235000009566 rice Nutrition 0.000 description 17
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 16
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 16
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 16
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 15
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 13
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 13
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 12
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 12
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 12
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 12
- 239000011159 matrix material Substances 0.000 description 12
- 239000003921 oil Substances 0.000 description 12
- 235000019198 oils Nutrition 0.000 description 12
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 11
- 235000004279 alanine Nutrition 0.000 description 11
- 238000010586 diagram Methods 0.000 description 11
- 239000000463 material Substances 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 10
- 240000008042 Zea mays Species 0.000 description 10
- 235000003704 aspartic acid Nutrition 0.000 description 10
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 10
- 239000003925 fat Substances 0.000 description 10
- 235000019197 fats Nutrition 0.000 description 10
- 235000013922 glutamic acid Nutrition 0.000 description 10
- 239000004220 glutamic acid Substances 0.000 description 10
- 229930182817 methionine Natural products 0.000 description 10
- 210000001519 tissue Anatomy 0.000 description 10
- 241000218976 Populus trichocarpa Species 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 241000589158 Agrobacterium Species 0.000 description 8
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 8
- 235000009582 asparagine Nutrition 0.000 description 8
- 229960001230 asparagine Drugs 0.000 description 8
- 239000002299 complementary DNA Substances 0.000 description 8
- GNGACRATGGDKBX-UHFFFAOYSA-N dihydroxyacetone phosphate Chemical compound OCC(=O)COP(O)(O)=O GNGACRATGGDKBX-UHFFFAOYSA-N 0.000 description 8
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 8
- 230000009466 transformation Effects 0.000 description 8
- 101100406701 Arabidopsis thaliana At3g16800 gene Proteins 0.000 description 7
- 240000002791 Brassica napus Species 0.000 description 7
- 239000004472 Lysine Substances 0.000 description 7
- 240000003768 Solanum lycopersicum Species 0.000 description 7
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 7
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 6
- 240000008100 Brassica rapa Species 0.000 description 6
- 235000011292 Brassica rapa Nutrition 0.000 description 6
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 6
- 241000209510 Liliopsida Species 0.000 description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 235000005822 corn Nutrition 0.000 description 6
- 229930027917 kanamycin Natural products 0.000 description 6
- 229960000318 kanamycin Drugs 0.000 description 6
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 6
- 229930182823 kanamycin A Natural products 0.000 description 6
- 230000035772 mutation Effects 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000013518 transcription Methods 0.000 description 6
- 230000035897 transcription Effects 0.000 description 6
- 241000219194 Arabidopsis Species 0.000 description 5
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 101150095274 FBA1 gene Proteins 0.000 description 5
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 5
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 5
- 244000061176 Nicotiana tabacum Species 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 240000000111 Saccharum officinarum Species 0.000 description 5
- 235000007201 Saccharum officinarum Nutrition 0.000 description 5
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 5
- 239000004473 Threonine Substances 0.000 description 5
- 235000014787 Vitis vinifera Nutrition 0.000 description 5
- 240000006365 Vitis vinifera Species 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 230000002103 transcriptional effect Effects 0.000 description 5
- 239000010455 vermiculite Substances 0.000 description 5
- 229910052902 vermiculite Inorganic materials 0.000 description 5
- 235000019354 vermiculite Nutrition 0.000 description 5
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 4
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 4
- 101100055297 Arabidopsis thaliana FBA2 gene Proteins 0.000 description 4
- 239000004475 Arginine Substances 0.000 description 4
- 241000219193 Brassicaceae Species 0.000 description 4
- 240000001980 Cucurbita pepo Species 0.000 description 4
- 206010059866 Drug resistance Diseases 0.000 description 4
- 239000004471 Glycine Substances 0.000 description 4
- 241000219828 Medicago truncatula Species 0.000 description 4
- 235000002731 Mimulus guttatus Nutrition 0.000 description 4
- 241000218595 Picea sitchensis Species 0.000 description 4
- 241000209504 Poaceae Species 0.000 description 4
- 244000044283 Toxicodendron succedaneum Species 0.000 description 4
- 235000007244 Zea mays Nutrition 0.000 description 4
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 4
- 239000003225 biodiesel Substances 0.000 description 4
- 238000010276 construction Methods 0.000 description 4
- 241001233957 eudicotyledons Species 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 231100000350 mutagenesis Toxicity 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000003259 recombinant expression Methods 0.000 description 4
- 230000019491 signal transduction Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 4
- LXJXRIRHZLFYRP-VKHMYHEASA-L (R)-2-Hydroxy-3-(phosphonooxy)-propanal Natural products O=C[C@H](O)COP([O-])([O-])=O LXJXRIRHZLFYRP-VKHMYHEASA-L 0.000 description 3
- 101100135280 Arabidopsis thaliana At1g16220 gene Proteins 0.000 description 3
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 3
- 241000701489 Cauliflower mosaic virus Species 0.000 description 3
- 241000195597 Chlamydomonas reinhardtii Species 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- 235000009852 Cucurbita pepo Nutrition 0.000 description 3
- XPYBSIWDXQFNMH-UHFFFAOYSA-N D-fructose 1,6-bisphosphate Natural products OP(=O)(O)OCC(O)C(O)C(O)C(=O)COP(O)(O)=O XPYBSIWDXQFNMH-UHFFFAOYSA-N 0.000 description 3
- LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 3
- 244000127993 Elaeis melanococca Species 0.000 description 3
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 3
- 244000020551 Helianthus annuus Species 0.000 description 3
- 235000003222 Helianthus annuus Nutrition 0.000 description 3
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
- 241000219823 Medicago Species 0.000 description 3
- 244000021685 Mesembryanthemum crystallinum Species 0.000 description 3
- 235000009071 Mesembryanthemum crystallinum Nutrition 0.000 description 3
- 240000003433 Miscanthus floridulus Species 0.000 description 3
- 241000195887 Physcomitrella patens Species 0.000 description 3
- 240000000528 Ricinus communis Species 0.000 description 3
- 235000004443 Ricinus communis Nutrition 0.000 description 3
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 3
- 241000219095 Vitis Species 0.000 description 3
- 235000009392 Vitis Nutrition 0.000 description 3
- 125000001931 aliphatic group Chemical group 0.000 description 3
- RNBGYGVWRKECFJ-ZXXMMSQZSA-N alpha-D-fructofuranose 1,6-bisphosphate Chemical compound O[C@H]1[C@H](O)[C@](O)(COP(O)(O)=O)O[C@@H]1COP(O)(O)=O RNBGYGVWRKECFJ-ZXXMMSQZSA-N 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 125000003118 aryl group Chemical group 0.000 description 3
- 238000002869 basic local alignment search tool Methods 0.000 description 3
- 239000002551 biofuel Substances 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 235000018417 cysteine Nutrition 0.000 description 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- RNBGYGVWRKECFJ-UHFFFAOYSA-N fructose-1,6-phosphate Natural products OC1C(O)C(O)(COP(O)(O)=O)OC1COP(O)(O)=O RNBGYGVWRKECFJ-UHFFFAOYSA-N 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000008595 infiltration Effects 0.000 description 3
- 238000001764 infiltration Methods 0.000 description 3
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 description 3
- 238000002703 mutagenesis Methods 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 3
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 210000002706 plastid Anatomy 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 230000005030 transcription termination Effects 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 108010085238 Actins Proteins 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 244000144725 Amygdalus communis Species 0.000 description 2
- 235000011437 Amygdalus communis Nutrition 0.000 description 2
- 244000226021 Anacardium occidentale Species 0.000 description 2
- 101100055296 Arabidopsis thaliana FBA1 gene Proteins 0.000 description 2
- 101100244158 Arabidopsis thaliana PLPZETA2 gene Proteins 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000011293 Brassica napus Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 235000013162 Cocos nucifera Nutrition 0.000 description 2
- 244000060011 Cocos nucifera Species 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 241000190106 Cyanidioschyzon merolae Species 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 235000009355 Dianthus caryophyllus Nutrition 0.000 description 2
- 240000006497 Dianthus caryophyllus Species 0.000 description 2
- 235000001950 Elaeis guineensis Nutrition 0.000 description 2
- PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 description 2
- 241000220485 Fabaceae Species 0.000 description 2
- 239000005980 Gibberellic acid Substances 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 125000002842 L-seryl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])O[H] 0.000 description 2
- 241000234435 Lilium Species 0.000 description 2
- 235000011430 Malus pumila Nutrition 0.000 description 2
- 235000014826 Mangifera indica Nutrition 0.000 description 2
- 240000007228 Mangifera indica Species 0.000 description 2
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
- 244000016966 Mimulus guttatus Species 0.000 description 2
- 244000171805 Mimulus langsdorfii Species 0.000 description 2
- 235000013667 Mimulus langsdorfii Nutrition 0.000 description 2
- 241001230286 Narenga Species 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 101150099368 Os05g0358500 gene Proteins 0.000 description 2
- 235000006089 Phaseolus angularis Nutrition 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010659 Phoenix dactylifera Nutrition 0.000 description 2
- 244000104275 Phoenix dactylifera Species 0.000 description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- 235000003447 Pistacia vera Nutrition 0.000 description 2
- 240000006711 Pistacia vera Species 0.000 description 2
- 240000004713 Pisum sativum Species 0.000 description 2
- 235000010582 Pisum sativum Nutrition 0.000 description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
- 235000019484 Rapeseed oil Nutrition 0.000 description 2
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 2
- 235000019485 Safflower oil Nutrition 0.000 description 2
- 241000218998 Salicaceae Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000208292 Solanaceae Species 0.000 description 2
- 235000002560 Solanum lycopersicum Nutrition 0.000 description 2
- 235000002597 Solanum melongena Nutrition 0.000 description 2
- 244000061458 Solanum melongena Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 235000019486 Sunflower oil Nutrition 0.000 description 2
- 241000723873 Tobacco mosaic virus Species 0.000 description 2
- 244000098338 Triticum aestivum Species 0.000 description 2
- 108090000848 Ubiquitin Proteins 0.000 description 2
- 235000010749 Vicia faba Nutrition 0.000 description 2
- 240000006677 Vicia faba Species 0.000 description 2
- 240000007098 Vigna angularis Species 0.000 description 2
- 235000010711 Vigna angularis Nutrition 0.000 description 2
- 244000195452 Wasabia japonica Species 0.000 description 2
- 235000000760 Wasabia japonica Nutrition 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 238000007792 addition Methods 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 125000003368 amide group Chemical group 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 239000003240 coconut oil Substances 0.000 description 2
- 235000019864 coconut oil Nutrition 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 235000005687 corn oil Nutrition 0.000 description 2
- 239000002285 corn oil Substances 0.000 description 2
- 235000012343 cottonseed oil Nutrition 0.000 description 2
- 239000002385 cottonseed oil Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000010413 gardening Methods 0.000 description 2
- 238000012239 gene modification Methods 0.000 description 2
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 2
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 125000002883 imidazolyl group Chemical group 0.000 description 2
- 125000001841 imino group Chemical group [H]N=* 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229950006238 nadide Drugs 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 108010058731 nopaline synthase Proteins 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 239000004006 olive oil Substances 0.000 description 2
- 235000008390 olive oil Nutrition 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 235000005713 safflower oil Nutrition 0.000 description 2
- 239000003813 safflower oil Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000010153 self-pollination Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000008159 sesame oil Substances 0.000 description 2
- 235000011803 sesame oil Nutrition 0.000 description 2
- 239000003549 soybean oil Substances 0.000 description 2
- 235000012424 soybean oil Nutrition 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000002600 sunflower oil Substances 0.000 description 2
- 238000011426 transformation method Methods 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 108700026220 vif Genes Proteins 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 101150084750 1 gene Proteins 0.000 description 1
- MWBWWFOAEOYUST-UHFFFAOYSA-N 2-aminopurine Chemical compound NC1=NC=C2N=CNC2=N1 MWBWWFOAEOYUST-UHFFFAOYSA-N 0.000 description 1
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- LQLQRFGHAALLLE-UHFFFAOYSA-N 5-bromouracil Chemical compound BrC1=CNC(=O)NC1=O LQLQRFGHAALLLE-UHFFFAOYSA-N 0.000 description 1
- 102100023826 ADP-ribosylation factor 4 Human genes 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 235000003840 Amygdalus nana Nutrition 0.000 description 1
- 241000208223 Anacardiaceae Species 0.000 description 1
- 235000001274 Anacardium occidentale Nutrition 0.000 description 1
- 108010037365 Arabidopsis Proteins Proteins 0.000 description 1
- 101100296065 Arabidopsis thaliana At1g79630 gene Proteins 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 241000233788 Arecaceae Species 0.000 description 1
- 241000208838 Asteraceae Species 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 244000060924 Brassica campestris Species 0.000 description 1
- 235000005637 Brassica campestris Nutrition 0.000 description 1
- 244000026811 Brassica nipposinica Species 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 244000178937 Brassica oleracea var. capitata Species 0.000 description 1
- 235000008744 Brassica perviridis Nutrition 0.000 description 1
- 244000233513 Brassica perviridis Species 0.000 description 1
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 241001604477 Brassica rapa var. rapa Species 0.000 description 1
- 235000010570 Brassica rapa var. rapa Nutrition 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 235000002567 Capsicum annuum Nutrition 0.000 description 1
- 240000004160 Capsicum annuum Species 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000219321 Caryophyllaceae Species 0.000 description 1
- 241000723353 Chrysanthemum Species 0.000 description 1
- 235000009604 Chrysanthemum X morifolium Nutrition 0.000 description 1
- 235000005633 Chrysanthemum balsamita Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 241001677238 Copernicia Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 241000219104 Cucurbitaceae Species 0.000 description 1
- YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 235000018060 Elaeis melanococca Nutrition 0.000 description 1
- 101150055254 FBA2 gene Proteins 0.000 description 1
- 101150115574 FBA3 gene Proteins 0.000 description 1
- 241000220223 Fragaria Species 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 102000005720 Glutathione transferase Human genes 0.000 description 1
- 108010070675 Glutathione transferase Proteins 0.000 description 1
- 102000000587 Glycerolphosphate Dehydrogenase Human genes 0.000 description 1
- 108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 101000684189 Homo sapiens ADP-ribosylation factor 4 Proteins 0.000 description 1
- 101150017040 I gene Proteins 0.000 description 1
- 238000012218 Kunkel's method Methods 0.000 description 1
- 240000007741 Lagenaria siceraria Species 0.000 description 1
- 235000009797 Lagenaria vulgaris Nutrition 0.000 description 1
- 240000007472 Leucaena leucocephala Species 0.000 description 1
- 235000010643 Leucaena leucocephala Nutrition 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 241000215452 Lotus corniculatus Species 0.000 description 1
- 241001480167 Lotus japonicus Species 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- 244000081841 Malus domestica Species 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000010624 Medicago sativa Nutrition 0.000 description 1
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 1
- 101710202365 Napin Proteins 0.000 description 1
- 101100242406 Oryza sativa subsp. japonica Os02g0224100 gene Proteins 0.000 description 1
- 101100242410 Oryza sativa subsp. japonica Os02g0471500 gene Proteins 0.000 description 1
- 101100406696 Oryza sativa subsp. japonica Os04g0321800 gene Proteins 0.000 description 1
- 101100082025 Oryza sativa subsp. japonica Os07g0566200 gene Proteins 0.000 description 1
- 101100082029 Oryza sativa subsp. japonica Os08g0500300 gene Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 241000209117 Panicum Species 0.000 description 1
- 235000006443 Panicum miliaceum subsp. miliaceum Nutrition 0.000 description 1
- 235000009037 Panicum miliaceum subsp. ruderale Nutrition 0.000 description 1
- 241001520808 Panicum virgatum Species 0.000 description 1
- 241000209046 Pennisetum Species 0.000 description 1
- 244000130556 Pennisetum purpureum Species 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 241000745988 Phyllostachys Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 240000003889 Piper guineense Species 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000218982 Populus nigra Species 0.000 description 1
- 241000183024 Populus tremula Species 0.000 description 1
- 241000549435 Pria Species 0.000 description 1
- 235000011432 Prunus Nutrition 0.000 description 1
- 241000220299 Prunus Species 0.000 description 1
- 241001290151 Prunus avium subsp. avium Species 0.000 description 1
- 235000019057 Raphanus caudatus Nutrition 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000011380 Raphanus sativus Nutrition 0.000 description 1
- 235000005733 Raphanus sativus var niger Nutrition 0.000 description 1
- 244000155437 Raphanus sativus var. niger Species 0.000 description 1
- 235000003846 Ricinus Nutrition 0.000 description 1
- 241000322381 Ricinus <louse> Species 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 240000003829 Sorghum propinquum Species 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000008049 TAE buffer Substances 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 235000008326 Trichosanthes anguina Nutrition 0.000 description 1
- 244000078912 Trichosanthes cucumerina Species 0.000 description 1
- 240000000890 Trichosanthes cucumeroides Species 0.000 description 1
- 235000018854 Trichosanthes cucumeroides Nutrition 0.000 description 1
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 1
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000722923 Tulipa Species 0.000 description 1
- 241000722921 Tulipa gesneriana Species 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 241000219995 Wisteria Species 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 244000042312 Wisteria floribunda Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 239000001511 capsicum annuum Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 235000020226 cashew nut Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 235000002532 grape seed extract Nutrition 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000010884 ion-beam technique Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 238000002887 multiple sequence alignment Methods 0.000 description 1
- 125000004433 nitrogen atom Chemical group N* 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 235000020233 pistachio Nutrition 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 235000014774 prunus Nutrition 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009-060154 | 2009-03-12 | ||
| JP2009060154A JP5604657B2 (ja) | 2009-03-12 | 2009-03-12 | 植物のバイオマス量及び／又は種子量を増産させる遺伝子及びその利用方法 |
| PCT/JP2010/053939 WO2010104092A1 (ja) | 2009-03-12 | 2010-03-10 | 植物のバイオマス量及び／又は種子量を増産させる遺伝子及びその利用方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2010222106A1 AU2010222106A1 (en) | 2011-11-03 |
| AU2010222106B2 true AU2010222106B2 (en) | 2013-08-29 |
Family
ID=42728381
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2010222106A Ceased AU2010222106B2 (en) | 2009-03-12 | 2010-03-10 | Gene for increasing the production of plant biomass and/or seeds and method for use thereof |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9546376B2 (https=) |
| JP (1) | JP5604657B2 (https=) |
| CN (1) | CN102395675A (https=) |
| AU (1) | AU2010222106B2 (https=) |
| BR (1) | BRPI1009784A2 (https=) |
| WO (1) | WO2010104092A1 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110078818A1 (en) * | 2008-03-14 | 2011-03-31 | Satoshi Kondo | Gene for increasing the production of plant biomass and/or seeds and method for use thereof |
| JP5604657B2 (ja) | 2009-03-12 | 2014-10-08 | トヨタ自動車株式会社 | 植物のバイオマス量及び／又は種子量を増産させる遺伝子及びその利用方法 |
| WO2012039159A1 (ja) * | 2010-09-22 | 2012-03-29 | 国立大学法人奈良先端科学技術大学院大学 | 塊茎生産能または匍匐枝形成能が野生株に比して向上している匍匐枝形成植物の作製方法、当該方法によって作製された匍匐枝形成植物 |
| US9238819B2 (en) * | 2011-05-04 | 2016-01-19 | Versitech Limited | Method for speeding up plant growth and improving yield by altering expression levels of kinases and phosphatases |
| JP6397610B2 (ja) | 2013-09-10 | 2018-09-26 | 株式会社豊田中央研究所 | 師部組織増産に適した植物体及びその利用 |
| CN115028696B (zh) * | 2021-02-20 | 2023-05-02 | 中国农业大学 | 一种与果实品质相关的蛋白及其编码基因的应用 |
| CN118703533B (zh) * | 2024-08-01 | 2025-11-21 | 湖南农业大学 | OsGAT3基因在调控水稻Cd、Cu或Mn含量及株高中的应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007091634A1 (ja) * | 2006-02-09 | 2007-08-16 | Japan Science And Technology Agency | 成長性および病虫害抵抗性が向上した植物、並びにその作出方法 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0722494B1 (en) | 1993-10-06 | 2006-04-26 | New York University | Method for producing transgenic plants that exhibit enhanced nitrogen assimilation |
| WO1998010082A1 (en) | 1994-09-01 | 1998-03-12 | University Of Florida Research Foundation, Inc. | Materials and methods for increasing corn seed weight |
| US6252139B1 (en) | 1996-07-18 | 2001-06-26 | The Salk Institute For Biological Studies | Method of increasing growth and yield in plants |
| BR9612725A (pt) | 1996-09-05 | 1999-08-24 | Univ Florida | Materiais e processos para aumentar o peso da semente de milho |
| BR9807886A (pt) | 1997-03-26 | 2000-02-22 | Univ Cambridge Tech | Processo para se obter uma planta com caracterìsticas de crescimento alteradas ou arquitetura alterada, gene quimérico, célula de planta, planta, semente da mesma, fragmento isolado de dna, e, usos de uma proteìna que controla a divisão celular, e de um dna que codifica a mesma |
| US7598361B2 (en) * | 1997-11-24 | 2009-10-06 | Monsanto Technology Llc | Nucleic acid molecules and other molecules associated with the sucrose pathway |
| US20100293663A2 (en) | 1998-06-16 | 2010-11-18 | Thomas La Rosa | Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement |
| US20020040490A1 (en) | 2000-01-27 | 2002-04-04 | Jorn Gorlach | Expressed sequences of arabidopsis thaliana |
| US7109033B2 (en) | 2000-08-24 | 2006-09-19 | The Scripps Research Institute | Stress-regulated genes of plants, transgenic plants containing same, and methods of use |
| WO2002010210A2 (en) * | 2001-08-28 | 2002-02-07 | Bayer Cropscience Ag | Polypeptides for identifying herbicidally active compounds |
| JP2005185101A (ja) | 2002-05-30 | 2005-07-14 | National Institute Of Agrobiological Sciences | 植物の全長ｃＤＮＡおよびその利用 |
| US20070136839A1 (en) | 2003-10-14 | 2007-06-14 | Ceres, Inc. | Promoter, promoter control elements, and combinations, and uses thereof |
| JP2005130770A (ja) | 2003-10-30 | 2005-05-26 | Ajinomoto Co Inc | 植物体あたり得られるデンプン量が増加したバレイショおよびその作出法 |
| EP1732378B1 (en) | 2004-04-02 | 2013-01-16 | CropDesign N.V. | Plants overexpressing a NAP1-like protein with increased seed yield a method for making the same |
| AU2006204997B2 (en) * | 2005-01-12 | 2011-09-01 | Monsanto Technology, Llc | Genes and uses for plant improvement |
| EP2090662A3 (en) * | 2006-04-05 | 2012-10-31 | Metanomics GmbH | Process for the production of a fine chemical |
| PL2103212T3 (pl) * | 2006-12-11 | 2015-03-31 | Okayama Barley Genome Tech Co Ltd | Regulator wzrostu roślin i jego zastosowanie |
| US20080261913A1 (en) | 2006-12-28 | 2008-10-23 | Idenix Pharmaceuticals, Inc. | Compounds and pharmaceutical compositions for the treatment of liver disorders |
| MX2009007556A (es) | 2007-01-16 | 2009-08-12 | Japan Science & Tech Agency | Planta con rendimiento incrementado de semillas. |
| US20110078818A1 (en) | 2008-03-14 | 2011-03-31 | Satoshi Kondo | Gene for increasing the production of plant biomass and/or seeds and method for use thereof |
| JP5604657B2 (ja) | 2009-03-12 | 2014-10-08 | トヨタ自動車株式会社 | 植物のバイオマス量及び／又は種子量を増産させる遺伝子及びその利用方法 |
| JP5519192B2 (ja) | 2009-06-04 | 2014-06-11 | トヨタ自動車株式会社 | 種子のタンパク質含量を増産させる遺伝子及びその利用方法 |
| JP5250807B2 (ja) | 2009-09-11 | 2013-07-31 | トヨタ自動車株式会社 | 植物のバイオマス量及び／又は種子量を増産させる方法、バイオマス量及び／又は種子量を増産できる植物の製造方法 |
-
2009
- 2009-03-12 JP JP2009060154A patent/JP5604657B2/ja not_active Expired - Fee Related
-
2010
- 2010-03-10 CN CN2010800113921A patent/CN102395675A/zh active Pending
- 2010-03-10 BR BRPI1009784-8A patent/BRPI1009784A2/pt not_active Application Discontinuation
- 2010-03-10 US US13/256,190 patent/US9546376B2/en not_active Expired - Fee Related
- 2010-03-10 WO PCT/JP2010/053939 patent/WO2010104092A1/ja not_active Ceased
- 2010-03-10 AU AU2010222106A patent/AU2010222106B2/en not_active Ceased
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007091634A1 (ja) * | 2006-02-09 | 2007-08-16 | Japan Science And Technology Agency | 成長性および病虫害抵抗性が向上した植物、並びにその作出方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20120005787A1 (en) | 2012-01-05 |
| WO2010104092A1 (ja) | 2010-09-16 |
| BRPI1009784A2 (pt) | 2015-08-25 |
| JP5604657B2 (ja) | 2014-10-08 |
| JP2010207199A (ja) | 2010-09-24 |
| AU2010222106A1 (en) | 2011-11-03 |
| CN102395675A (zh) | 2012-03-28 |
| US9546376B2 (en) | 2017-01-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10287604B2 (en) | Gene for increasing the production of plant biomass and/or seeds and method for use thereof | |
| US8575428B2 (en) | Method for increasing the production of plant biomass and/or seeds and method for producing plant capable of producing increased amount of biomass and/or seeds | |
| US9476059B2 (en) | Gene capable of imparting environmental stress resistance to plants and method for utilizing the same | |
| AU2010222106B2 (en) | Gene for increasing the production of plant biomass and/or seeds and method for use thereof | |
| CA2737735C (en) | Gene capable of increasing the production of plant biomass and method for using the same | |
| US9297020B2 (en) | Gene for increasing the production of plant biomass and method of use thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE INVENTION TITLE TO READ GENE FOR INCREASING THE PRODUCTION OF PLANT BIOMASS AND/OR SEEDS AND METHOD FOR USE THEREOF |
|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |