AU2009259664A1 - Method and apparatus for retaining and recirculating cells - Google Patents
Method and apparatus for retaining and recirculating cells Download PDFInfo
- Publication number
- AU2009259664A1 AU2009259664A1 AU2009259664A AU2009259664A AU2009259664A1 AU 2009259664 A1 AU2009259664 A1 AU 2009259664A1 AU 2009259664 A AU2009259664 A AU 2009259664A AU 2009259664 A AU2009259664 A AU 2009259664A AU 2009259664 A1 AU2009259664 A1 AU 2009259664A1
- Authority
- AU
- Australia
- Prior art keywords
- channels
- cells
- hollow cylinder
- bioreactor
- flow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims description 32
- 230000003134 recirculating effect Effects 0.000 title claims description 13
- 238000001816 cooling Methods 0.000 claims description 12
- 229920001296 polysiloxane Polymers 0.000 claims description 9
- 239000013049 sediment Substances 0.000 claims description 6
- 230000003247 decreasing effect Effects 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 142
- 239000007789 gas Substances 0.000 description 41
- 230000014759 maintenance of location Effects 0.000 description 36
- 238000004113 cell culture Methods 0.000 description 28
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 22
- 229910052760 oxygen Inorganic materials 0.000 description 22
- 239000001301 oxygen Substances 0.000 description 22
- 238000003306 harvesting Methods 0.000 description 13
- 238000009434 installation Methods 0.000 description 13
- 238000000855 fermentation Methods 0.000 description 12
- 230000004151 fermentation Effects 0.000 description 12
- 239000000463 material Substances 0.000 description 11
- 239000000853 adhesive Substances 0.000 description 9
- 230000001070 adhesive effect Effects 0.000 description 9
- 230000005484 gravity Effects 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- 239000002028 Biomass Substances 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 8
- 230000010412 perfusion Effects 0.000 description 8
- 238000004062 sedimentation Methods 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 239000012528 membrane Substances 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 230000032258 transport Effects 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 238000012423 maintenance Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000000717 retained effect Effects 0.000 description 6
- 238000007789 sealing Methods 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000012228 culture supernatant Substances 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 238000005304 joining Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
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- 239000012465 retentate Substances 0.000 description 3
- 238000004114 suspension culture Methods 0.000 description 3
- 210000004102 animal cell Anatomy 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
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- 230000004060 metabolic process Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
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- 208000011691 Burkitt lymphomas Diseases 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
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- 101000938351 Homo sapiens Ephrin type-A receptor 3 Proteins 0.000 description 1
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- 229910010293 ceramic material Inorganic materials 0.000 description 1
- 238000004581 coalescence Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000002826 coolant Substances 0.000 description 1
- 239000000110 cooling liquid Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
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- 230000004927 fusion Effects 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 102000057382 human EPHA3 Human genes 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 230000005501 phase interface Effects 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
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- 238000010008 shearing Methods 0.000 description 1
- 239000013464 silicone adhesive Substances 0.000 description 1
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- 239000004945 silicone rubber Substances 0.000 description 1
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- 230000007704 transition Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
- 239000002759 woven fabric Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
- C12M29/08—Air lift
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/22—Settling tanks; Sedimentation by gravity
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102008029307A DE102008029307A1 (de) | 2008-06-20 | 2008-06-20 | Verfahren und Vorrichtung zur Rückhaltung und Rückführung von Zellen |
| DE102008029307.5 | 2008-06-20 | ||
| PCT/EP2009/004200 WO2009152990A2 (de) | 2008-06-20 | 2009-06-10 | Verfahren und vorrichtung zur rückhaltung und rückführung von zellen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2009259664A1 true AU2009259664A1 (en) | 2009-12-23 |
Family
ID=41334928
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2009259664A Abandoned AU2009259664A1 (en) | 2008-06-20 | 2009-06-10 | Method and apparatus for retaining and recirculating cells |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20110097800A1 (https=) |
| EP (1) | EP2304017A2 (https=) |
| JP (1) | JP2011529331A (https=) |
| KR (1) | KR20110027696A (https=) |
| CN (1) | CN102239244B (https=) |
| AU (1) | AU2009259664A1 (https=) |
| CA (1) | CA2728453A1 (https=) |
| DE (1) | DE102008029307A1 (https=) |
| IL (1) | IL209232A0 (https=) |
| WO (1) | WO2009152990A2 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5820802B2 (ja) * | 2009-04-03 | 2015-11-24 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 組織及び臓器移植片のためのバイオリアクター並びに操作方法 |
| PL2665806T3 (pl) * | 2011-01-17 | 2021-12-27 | F.Hoffmann-La Roche Ag | Urządzenie rozdzielające |
| BR112014020120B1 (pt) * | 2012-02-20 | 2020-09-15 | Bayer Aktiengesellschaft | Separador de sólidos de canais inclinados para reter e recircular sólidos de uma mistura de um reator e unidade biorreatora |
| JP2015519922A (ja) * | 2012-06-21 | 2015-07-16 | ネオステム オンコロジー リミテッド ライビリティ カンパニー | バイオリアクタのカートリッジ及びシステム |
| JP2015077119A (ja) * | 2013-10-15 | 2015-04-23 | カンウォン ナショナル ユニバーシティ， ユニバーシティ−インダストリー コーペレーション ファンデーション | アクチノバシラススクシノゲネスを用いたコハク酸連続生産工程 |
| US10596492B2 (en) | 2014-07-09 | 2020-03-24 | Sudhin Biopharma | Particle settling devices |
| WO2016089874A1 (en) * | 2014-12-01 | 2016-06-09 | Sudhin Biopharma | Particle settling device with annular ramps |
| AU2019257236B2 (en) | 2018-04-18 | 2022-09-01 | Sudhin Biopharma | Particle settling devices |
| EP4121214A4 (en) | 2020-03-19 | 2024-07-10 | Sudhin Biopharma | Particle settling devices |
| WO2021199529A1 (ja) * | 2020-03-31 | 2021-10-07 | 昭和電工マテリアルズ株式会社 | 細胞培養装置及び培養方法 |
| DE102020118668A1 (de) * | 2020-07-15 | 2022-01-20 | Hochschule Anhalt (FH) , Körperschaft des öffentlichen Rechts | Fermentationsverfahren und Bioreaktor zur Durchführung von anaeroben, aeroben und mikro-aerophilen Fermentationen umfassend einen Airlift-Schachtüberfall |
| TWI751073B (zh) * | 2021-04-15 | 2021-12-21 | 蘇碩彥 | 交叉複合式傾斜管之製造方法與生產線 |
| WO2025027938A1 (ja) * | 2023-07-31 | 2025-02-06 | エイブル株式会社 | 培養装置及び細胞分離方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL76003A (en) | 1984-08-03 | 1988-12-30 | Biotechnolog Forschung Gmbh | Process and apparatus for the bubble-free gassing of liquids |
| US4814278A (en) * | 1985-02-05 | 1989-03-21 | Teijin Limited | Culture apparatus and method |
| GB8515636D0 (en) * | 1985-06-20 | 1985-07-24 | Celltech Ltd | Fermenter |
| DE3535183A1 (de) | 1985-10-02 | 1987-04-16 | Biotechnolog Forschung Gmbh | Vorrichtung und verfahren zur blasenfreien begasung von fluessigkeiten, insbesondere von kulturmedien zur vermehrung von gewebekulturen |
| US5320963A (en) * | 1992-11-25 | 1994-06-14 | National Research Council Of Canada | Bioreactor for the perfusion culture of cells |
| GB9309429D0 (en) | 1993-05-07 | 1993-06-23 | Bioscot Ltd | Fermenter accessory |
| KR100909316B1 (ko) | 2001-08-31 | 2009-07-24 | 바이엘 헬스케어 아게 | 고세포밀도 발효를 수행하기 위한 유닛 및 방법 |
| CN1286966C (zh) * | 2003-12-10 | 2006-11-29 | 上海伯瑞生物技术发展有限公司 | 造血细胞连续灌注悬浮培养过程细胞沉降截留装置 |
-
2008
- 2008-06-20 DE DE102008029307A patent/DE102008029307A1/de not_active Withdrawn
-
2009
- 2009-06-10 CN CN200980123039.XA patent/CN102239244B/zh not_active Expired - Fee Related
- 2009-06-10 JP JP2011513926A patent/JP2011529331A/ja not_active Withdrawn
- 2009-06-10 CA CA2728453A patent/CA2728453A1/en not_active Abandoned
- 2009-06-10 AU AU2009259664A patent/AU2009259664A1/en not_active Abandoned
- 2009-06-10 KR KR1020107028446A patent/KR20110027696A/ko not_active Withdrawn
- 2009-06-10 WO PCT/EP2009/004200 patent/WO2009152990A2/de not_active Ceased
- 2009-06-10 US US12/997,281 patent/US20110097800A1/en not_active Abandoned
- 2009-06-10 EP EP09765570A patent/EP2304017A2/de not_active Withdrawn
-
2010
- 2010-11-10 IL IL209232A patent/IL209232A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009152990A2 (de) | 2009-12-23 |
| CN102239244B (zh) | 2014-10-29 |
| CN102239244A (zh) | 2011-11-09 |
| US20110097800A1 (en) | 2011-04-28 |
| DE102008029307A1 (de) | 2009-12-24 |
| JP2011529331A (ja) | 2011-12-08 |
| WO2009152990A3 (de) | 2011-11-24 |
| EP2304017A2 (de) | 2011-04-06 |
| KR20110027696A (ko) | 2011-03-16 |
| IL209232A0 (en) | 2011-01-31 |
| CA2728453A1 (en) | 2009-12-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC1 | Assignment before grant (sect. 113) |
Owner name: BAYER INTELLECTUAL PROPERTY GMBH Free format text: FORMER APPLICANT(S): BAYER TECHNOLOGY SERVICES GMBH |
|
| MK1 | Application lapsed section 142(2)(a) - no request for examination in relevant period |