AU2006347573B2 - Synthetic genomes - Google Patents

Synthetic genomes Download PDF

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Publication number
AU2006347573B2
AU2006347573B2 AU2006347573A AU2006347573A AU2006347573B2 AU 2006347573 B2 AU2006347573 B2 AU 2006347573B2 AU 2006347573 A AU2006347573 A AU 2006347573A AU 2006347573 A AU2006347573 A AU 2006347573A AU 2006347573 B2 AU2006347573 B2 AU 2006347573B2
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AU
Australia
Prior art keywords
genome
synthetic
cassettes
cell
nucleic acid
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Active
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AU2006347573A
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English (en)
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AU2006347573A1 (en
Inventor
Daniel G. Gibson
Clyde A. Hutchison Iii
Hamilton O. Smith
Craig J. Venter
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Viridos Inc
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Synthetic Genomics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2006347573A 2005-12-06 2006-12-06 Synthetic genomes Active AU2006347573B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74254205P 2005-12-06 2005-12-06
US60/742,542 2005-12-06
PCT/US2006/046803 WO2008024129A2 (en) 2005-12-06 2006-12-06 Synthetic genomes

Publications (2)

Publication Number Publication Date
AU2006347573A1 AU2006347573A1 (en) 2008-02-28
AU2006347573B2 true AU2006347573B2 (en) 2013-01-17

Family

ID=39107256

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2006347573A Active AU2006347573B2 (en) 2005-12-06 2006-12-06 Synthetic genomes

Country Status (9)

Country Link
US (2) US10041060B2 (https=)
EP (1) EP1968994B1 (https=)
JP (1) JP5106412B2 (https=)
CN (1) CN101501207B (https=)
AU (1) AU2006347573B2 (https=)
CA (1) CA2643356A1 (https=)
DK (1) DK1968994T3 (https=)
IL (1) IL192041A (https=)
WO (1) WO2008024129A2 (https=)

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US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
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US9259662B2 (en) 2008-02-22 2016-02-16 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US10093552B2 (en) 2008-02-22 2018-10-09 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US8986963B2 (en) * 2008-02-23 2015-03-24 James Weifu Lee Designer calvin-cycle-channeled production of butanol and related higher alcohols
PL2250276T3 (pl) 2008-02-23 2017-07-31 James Weifu Lee Organizmy zaprojektowane do fotobiologicznego wytwarzania butanolu z ditlenku węgla i wody
CA2728285A1 (en) 2008-03-03 2009-09-11 Joule Unlimited, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
CN103725717A (zh) 2008-10-17 2014-04-16 焦耳无限科技公司 微生物的乙醇生产
CA2747535C (en) 2008-12-18 2020-01-14 Iti Scotland Limited Method for assembly of polynucleic acid sequences
SG10201400436PA (en) * 2009-03-06 2014-06-27 Synthetic Genomics Inc Methods For Cloning And Manipulating Genomes
US7794969B1 (en) 2009-07-09 2010-09-14 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
CN105969710A (zh) 2009-09-25 2016-09-28 Reg生命科学有限责任公司 脂肪酸衍生物的产生
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
EP2494060B1 (en) * 2009-10-30 2016-04-27 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
EP2542676A1 (en) * 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
WO2012037285A2 (en) 2010-09-14 2012-03-22 Joule Unlimited Technologies, Inc. Methods and compositions for targeting heterologous integral membrane proteins to the cyanobacterial plasma membrane
US8349587B2 (en) 2011-10-31 2013-01-08 Ginkgo Bioworks, Inc. Methods and systems for chemoautotrophic production of organic compounds
US8790901B2 (en) 2011-12-14 2014-07-29 Pronutria, Inc. Microorganisms and methods for producing unsaturated fatty acids
HK1206761A1 (en) 2012-03-26 2016-01-15 Axcella Health Inc. Nutritive fragments, proteins and methods
EP2831100A4 (en) 2012-03-26 2016-02-10 Pronutria Inc NUTRIENT PROTEINS AND METHOD
MX2014011459A (es) 2012-03-26 2015-02-04 Pronutria Inc Proteinas nutritivas cargadas y metodos.
HK1206765A1 (en) 2012-03-26 2016-01-15 Axcella Health Inc. Nutritive fragments, proteins and methods
GB201219989D0 (en) 2012-11-06 2012-12-19 Discuva Ltd Bacterial engineering
US20150315599A1 (en) 2012-12-07 2015-11-05 Ginkgo Bioworks, Inc Methods and Systems for Methylotrophic Production of Organic Compounds
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP3048904A2 (en) 2013-09-25 2016-08-03 Pronutria Biosciences, Inc. Compositions and formulations for treatment of gastrointestinal tract malabsorption diseases and inflammatory conditions and methods of production and use thereof
WO2015048339A2 (en) 2013-09-25 2015-04-02 Pronutria, Inc. Compositions and formulations for non-human nutrition and methods of production and use thereof
WO2015054507A1 (en) 2013-10-10 2015-04-16 Pronutria, Inc. Nutritive polypeptide production systems, and methods of manufacture and use thereof
GB201413202D0 (en) 2014-07-25 2014-09-10 Discuva Ltd Process for producing bacterial mutants
EP3430133A1 (en) 2016-03-17 2019-01-23 INVISTA Textiles (U.K.) Limited Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto
EP3433354B1 (en) * 2016-03-23 2023-12-27 Synthetic Genomics, Inc. Generation of synthetic genomes
EP3577227A4 (en) 2017-02-02 2020-12-30 Cargill Inc. GENETICALLY MODIFIED CELLS PRODUCING C6-C10 FATTY ACID DERIVATIVES
US11512276B2 (en) 2018-03-30 2022-11-29 Inv Nylon Chemicals Americas, Llc Methods for controlling oxygen concentration during aerobic biosynthesis
EP3775240A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Method for controlling dissolved oxygen concentration in a continuous aerobic fermentation
EP3775182A1 (en) 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides
WO2019191767A1 (en) 2018-03-30 2019-10-03 Invista Textiles (U.K.) Limited High hydrogen utilization and gas recycle
US11999943B2 (en) 2018-05-02 2024-06-04 Inv Nylon Chemicals Americas, Llc Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-CoA-TCA balance in species of the genera ralstonia and cupriavidus and organisms related thereto
WO2019213118A1 (en) 2018-05-02 2019-11-07 Invista North America S.A.R.L. Methods for controlling pha biosynthesis in cupriavidus or ralstonia
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法

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US20030087238A1 (en) * 1997-09-16 2003-05-08 Evans Glen A. Method for assembly of a polynucleotide encoding a target polypeptide

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CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
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US20030087238A1 (en) * 1997-09-16 2003-05-08 Evans Glen A. Method for assembly of a polynucleotide encoding a target polypeptide
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide

Non-Patent Citations (1)

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Title
SMITH, H. O. et al., PNAS, 2003, vol. 100, no. 26, pages 15440-15445 *

Also Published As

Publication number Publication date
CN101501207B (zh) 2014-03-12
JP5106412B2 (ja) 2012-12-26
US20070264688A1 (en) 2007-11-15
IL192041A (en) 2013-08-29
CA2643356A1 (en) 2008-02-28
US10041060B2 (en) 2018-08-07
IL192041A0 (en) 2008-12-29
EP1968994A2 (en) 2008-09-17
AU2006347573A1 (en) 2008-02-28
WO2008024129A2 (en) 2008-02-28
JP2009518038A (ja) 2009-05-07
EP1968994B1 (en) 2013-07-03
CN101501207A (zh) 2009-08-05
DK1968994T3 (da) 2013-09-30
WO2008024129A3 (en) 2008-10-09
US20180340165A1 (en) 2018-11-29
EP1968994A4 (en) 2009-04-08

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DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE INVENTORS TO ADD: HUTCHISON, CLYDE A. III AND GIBSON, DANIEL G.

PC1 Assignment before grant (sect. 113)

Owner name: SYNTHETIC GENOMICS, INC.

Free format text: FORMER APPLICANT(S): J. CRAIG VENTER INSTITUTE

FGA Letters patent sealed or granted (standard patent)