AU2004243133B2 - New product - Google Patents
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- Publication number
- AU2004243133B2 AU2004243133B2 AU2004243133A AU2004243133A AU2004243133B2 AU 2004243133 B2 AU2004243133 B2 AU 2004243133B2 AU 2004243133 A AU2004243133 A AU 2004243133A AU 2004243133 A AU2004243133 A AU 2004243133A AU 2004243133 B2 AU2004243133 B2 AU 2004243133B2
- Authority
- AU
- Australia
- Prior art keywords
- polymer matrix
- single layer
- lactic acid
- matrix film
- layer polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 166
- 229920000642 polymer Polymers 0.000 claims description 150
- 241000894006 Bacteria Species 0.000 claims description 114
- 239000011159 matrix material Substances 0.000 claims description 109
- 239000004310 lactic acid Substances 0.000 claims description 83
- 235000014655 lactic acid Nutrition 0.000 claims description 83
- 230000001580 bacterial effect Effects 0.000 claims description 34
- 239000002356 single layer Substances 0.000 claims description 22
- 238000003860 storage Methods 0.000 claims description 16
- 238000001035 drying Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000010410 layer Substances 0.000 claims description 10
- 229920002472 Starch Polymers 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 9
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 9
- 239000008107 starch Substances 0.000 claims description 9
- 235000019698 starch Nutrition 0.000 claims description 9
- 210000001519 tissue Anatomy 0.000 claims description 9
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 8
- 239000006185 dispersion Substances 0.000 claims description 8
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 206010021639 Incontinence Diseases 0.000 claims description 7
- 241000186660 Lactobacillus Species 0.000 claims description 7
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 7
- 210000001124 body fluid Anatomy 0.000 claims description 7
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 6
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 6
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 4
- 229920001477 hydrophilic polymer Polymers 0.000 claims description 4
- 230000008021 deposition Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 239000002085 irritant Substances 0.000 claims description 3
- 231100000021 irritant Toxicity 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
- 210000004400 mucous membrane Anatomy 0.000 claims description 3
- 231100000252 nontoxic Toxicity 0.000 claims description 3
- 230000003000 nontoxic effect Effects 0.000 claims description 3
- 241000194036 Lactococcus Species 0.000 claims description 2
- 241000192001 Pediococcus Species 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 238000010030 laminating Methods 0.000 claims 1
- 239000000047 product Substances 0.000 description 59
- 230000002745 absorbent Effects 0.000 description 28
- 239000002250 absorbent Substances 0.000 description 28
- 239000000463 material Substances 0.000 description 19
- 230000004083 survival effect Effects 0.000 description 18
- 238000012546 transfer Methods 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 13
- 230000000813 microbial effect Effects 0.000 description 9
- 208000015181 infectious disease Diseases 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 7
- 238000010521 absorption reaction Methods 0.000 description 6
- 239000000835 fiber Substances 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 230000032258 transport Effects 0.000 description 5
- 239000000853 adhesive Substances 0.000 description 4
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- 238000012360 testing method Methods 0.000 description 4
- 230000035899 viability Effects 0.000 description 4
- 239000001993 wax Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 229920006254 polymer film Polymers 0.000 description 3
- -1 sucrose ester Chemical class 0.000 description 3
- 239000012209 synthetic fiber Substances 0.000 description 3
- 229920002994 synthetic fiber Polymers 0.000 description 3
- 239000004753 textile Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 229920000297 Rayon Polymers 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
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- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
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- 238000001125 extrusion Methods 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000006261 foam material Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 239000002648 laminated material Substances 0.000 description 2
- 238000003475 lamination Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
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- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 229920005862 polyol Polymers 0.000 description 2
- 150000003077 polyols Chemical class 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
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- 235000018291 probiotics Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
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- 239000004627 regenerated cellulose Substances 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 235000010356 sorbitol Nutrition 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- BVRIUXYMUSKBHG-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]aniline Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(N)C=C1 BVRIUXYMUSKBHG-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 241000207202 Gardnerella Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 241001134659 Lactobacillus curvatus Species 0.000 description 1
- 241000194035 Lactococcus lactis Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 101100337539 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) gim-1 gene Proteins 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 241000588769 Proteus <enterobacteria> Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- SZYSLWCAWVWFLT-UTGHZIEOSA-N [(2s,3s,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)-2-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxolan-2-yl]methyl octadecanoate Chemical compound O([C@@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@]1(COC(=O)CCCCCCCCCCCCCCCCC)O[C@H](CO)[C@@H](O)[C@@H]1O SZYSLWCAWVWFLT-UTGHZIEOSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000001414 amino alcohols Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 229940072107 ascorbate Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
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- 239000012459 cleaning agent Substances 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
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- 229940011871 estrogen Drugs 0.000 description 1
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- 229920001038 ethylene copolymer Polymers 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000000245 forearm Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
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- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
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- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000009245 menopause Effects 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- CRVGTESFCCXCTH-UHFFFAOYSA-N methyl diethanolamine Chemical compound OCCN(C)CCO CRVGTESFCCXCTH-UHFFFAOYSA-N 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
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- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
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- 239000008363 phosphate buffer Substances 0.000 description 1
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- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
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- 230000002829 reductive effect Effects 0.000 description 1
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- 229910001220 stainless steel Inorganic materials 0.000 description 1
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- 150000005846 sugar alcohols Polymers 0.000 description 1
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- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/36—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing microorganisms
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
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Description
WO 2004/105822 PCT/SE2004/000806 1 New product Technical field The present invention refers to a film-shaped polymer matrix comprising lactic acid producing bacteria in a film-shaped matrix consisting of polymer(s) that is nontoxic and non-irritant to a user's skin and mucous membranes. The invention also refers to a process for producing such a film-shaped polymer matrix and products containing it.
Background of the invention The urogenital area harbors a complex microbial ecosystem comprising more than different bacterial species (Hill et al., Scand. J. Urol. Nephrol. 1984;86 (suppl.) 23-29). The dominating species for fertile women in this area are lactic acid producing bacteria belonging to the genus Lactobacillus. These lactic acid producing members are important for retaining a healthy microbial flora in these areas, and act as probiotic bacteria with an antagonistic effect against pathogenic microbial species. Lactic acid producing bacteria inhibit growth and colonization by other microorganisms by occupying suitable niches for colonization, by forming biofilms and competing for available nutrients, thereby excluding colonization by harmful microorganisms. Also, the production of hydrogen peroxide, specific inhibiting substances, such as bacteriocines, and organic acids (including lactic acid and acetic acid) that lower the pH, inhibit colonization by other microorganisms.
The microbial ecosystem of a healthy individual can be disturbed by the use of antibiotics, during hormonal changes, such as during pregnancy or use of contraceptives with estrogen, during menstruation, after menopause, in people suffering from diabetes etc. Also, microorganisms may spread from the anus to the urogenital area, thereby causing infections. This results in a disturbance of the normal microbial flora and leaves the individual susceptible to microbial infections that cause vagini- WO 2004/105822 PCT/SE2004/000806 2 tis, urinary tract infections and ordinary skin infections. Microorganisms commonly associated with these kinds of infections belong to the genera Escherichia, Enterococcus, Psedomonas, Proteus, Klebsiella, Streptococcus, Staphylococcus, Gardnerella and Candida. Women are at particular risk due to their shorter distance between the anus and the urogenital tract; specially at risk are young women, who not yet have a well developed microflora in the urogenital area and older women, who no longer have a protective flora.
One way to reduce the problems with the kinds of infections described above is to have a good personal hygiene. However, excessive use of cleaning agents not only decrease the amount of harmful microbes, but can harm the beneficial microbial flora, again render it susceptible for pathogenic species to colonize and cause infections. Alternatively, administration of lactic acid producing bacteria to the urogenital area and the skin in order to outcompete pathogenic species and facilitate reestablishment and maintenance of a beneficial microbial flora in these areas, have been found to be a successful means to treat and prevent microbial infections.
It has been suggested that lactic acid producing bacteria can be delivered via absorbent products, such as diapers, sanitary napkins, panty liners and tampons, as described in, for example, in W092/13577, W097/02846, W099/17813, W099/45099 and WO00/35502.
A major problem with providing products intended to be used for transfer of lactic acid producing bacteria, is that the bacteria have to retain viability during transport and storage of the products. Lactic acid producing bacteria rapidly lose viability under moist conditions, and it is therefore important that the bacteria are not exposed to moisture. One way to partly overcome this problem in absorbent products provided with lactic acid producing bacteria has been to supply the products with the bacteria, drying said products to remove most of the moisture in them and providing the product in moisture impervious packages (W099/17813).
An alternative way to protect bacteria against moisture has been to disperse the bacteria in a hydrophobic substance (see e.g. US 4,518,696; WO 92/13577; WO 02/28446) which due to its hydrophobic character will prevent moisture to reach the embedded bacterial cells.
However, there is still a need to develop alternative ways of protecting lactic acid producing bacteria from moisture that are suitable for the intended administration of the bacteria to a subject and that can be stored for long time periods without loss of viability of the bacterial cells, and additionally allowing efficient transfer of the lactic acid producing bacteria to the user. In addition, there is still a need to develop manufacturing processes that are efficient and less expensive.
Summary of the invention The present inventors have surprisingly found an alternative way to protect lactic acid producing bacterial cells from moisture, thereby increasing bacterial survival during transport and storage. The presented solution also results in a high transfer of bacterial cells to the skin of a subject. The present invention provides a single layer polymer matrix film comprising lactic acid producing bacteria in a pharmaceutically acceptable polymer(s), which pharmaceutically acceptable polymer(s) is able to protect bacterial cells from moisture during storage, but which can be dissolved in bodily fluids. The invention also relates to a process for producing such a film-shaped polymer matrix comprising lactic acid producing bacteria and products comprising such a film-shaped polymer matrix comprising lactic acid producing bacteria.
WO 2004/105822 PCTSE2004/000806 4 Short description of the figures Fig. 1 depicts an illustrative example of an absorbent product, such as a sanitary napkin, diaper, panty liner, incontinence guard and the like comprising a filmshaped polymer matrix according to the present invention.
Fig. 2 shows a cross-section of the absorbent product depicted in Fig. 1 along the line II-II in Fig. 1.
Fig. 3 depicts an schematic illustration of a tampon comprising a film-shaped polymer matrix according to the present invention.
Fig. 4 shows a cross-section of the absorbent product depicted in Fig. 3 along the line IV-IV in Fig. 3.
Fig. 5 shows the survival of lactic acid producing bacteria in film-shaped polymer matrixes according to the present invention during long term storage.
Detailed description of the invention The present invention is concerned with the problem with maintaining bacterial viability in products comprising lactic acid producing bacteria from the time for manufacturing until use of the product and obtaining a satisfactory transfer of the bacteria from the product to the user. A factor of major importance for increasing bacterial survival during storage is that the bacteria have to be protected from moisture. The present inventors have surprisingly found that embedding the lactic acid producing bacteria in a film-shaped polymer matrix results in a greatly enhanced survival of the bacterial cells during storage.
WO 2004/105822 PCT/SE2004/000806 Polymers suitable for the film-shaped polymer matrix protect the bacterial cells from moisture during storage, but are dissolved in bodily fluids and therefore release the bacterial cells when exposed to wet or moist conditions. The film-shaped polymer matrix comprising the lactic acid producing bacteria according to the present invention is composed of at least one polymer that is non-toxic and non-irritant to a user's skin and mucous membranes and at least one lactic acid producing bacterial strain.
Polymers suitable for the present invention include natural hydrophilic polymers, such as polysaccharides and derivatives thereof, such as starch and cellulose (including their derivatives), proteins, hydrophilic polymers, such as synthetic hydrophilic polymers, such as acrylate-based polymers, polyethers, such as polyethyleneoxide, polyurethanes, polyamides, polyacrylnitrile, vinyl-based polymers, such as polyvinyl pyrrolidone and polyvinyl alcohol, etc.. Preferred polymers for the present invention include, but are not limited to, polyvinyl alcohol, polyethyleneoxide, polyvinyl pyrrolidone and starch. The polymer(s) form a film-shaped matrix that embed the bacteria and thereby protect them from moisture. The polymers can be used alone or in different combinations.
The film-shaped polymer matrix comprising lactic acid producing bacteria according to the present invention may also include additional components. Examples of such components include, but are not limited to, agents protecting the bacteria during the manufacturing of the polymer film, like carbohydrates, such as maltose, sucrose, trehalose, lactose, glucose and fructose, proteins, such as skim milk and albumin, amino acids, such as Na-glutamate, polyols, such as xylitol, mannitol and sorbitol, and antioxidants, such as Na-ascorbate. The majority of these agents may also act as nutrients for bacterial propagation once the polymer film is dissolved.
Additional components are also exemplified by plasticizers that can be added to a polymer film comprising starch, like polyhydric alcohols, such as glycerol, polyols, sorbitol and polyvinyl alcohols, sucrose ester, such as sucrose stearate, fatty acids, such as palmitic acid, lipids based on esterified fatty acids, such as monoglycerides, WO 2004/105822 PCT/SE2004/000806 6 diglycerides and triglycerides, waxes and amino alcohols, such as triethanolamine and N-methyl-diethanol amine, and enzymes, such as amylase.
The concentration of the polymer solution is preferably between 0.1-10 more preferably 0.5-7 most preferably 1-2 The thickness of the film-shaped polymer matrix comprising lactic acid producing bacteria is preferably utm-5 mm, more preferably 100 [m-1 mm and most preferably 500 pm-1 mm.
In order to increase bacterial survival the water activity of the film-shaped polymer matrix comprising lactic acid producing bacteria is 0.30 or below, preferably 0.25 or below and most preferably 0.20 or below during at least 3 months storage at 23 0
C
and 50% relative humidity.
The number of lactic acid producing bacteria in the film-shaped polymer matrix according to the present invention is preferably 107 -1014 CFU/g film, more preferably 109-1012 CFU/g film.
In order for the film-shaped polymer matrix to allow sufficient transfer of bacteria the film-shaped polymer matrix preferably dissolves as rapidly as possible when exposed to moist or wet conditions and in either case within 6 hours, preferably within 4 hours.
Lactic acid producing bacteria are chosen for the present invention due to their positive effect in preventing and treating microbial infection in the urogenital area and on the skin. The bacteria are preferably isolated from the natural flora of a healthy person, preferably the bacteria are isolated from the skin or urogenital area.
Preferred "lactic acid producing bacteria" for the object of the present invention include bacteria from the genera Lactobacillus, Lactococcus and Pediococcus. Preferably the selected bacteria are from the species Lactococcus lactis, Lactobacillus acidophilus, Lactobacillus curvatus or Lactobacillus plantarum. More preferably WO 2004/105822 PCT/SE2004/000806 7 the bacterial strain is selected from Lactobacillus plantarum. Even more preferably the lactic acid producing bacterium is Lactobacillus plantarum 931 (deposition No.
(DSMZ): 11918). The lactic acid producing bacteria can be provided alone or in mixtures containing at least two bacterial strains.
To prevent water-vapor to permeate into the hydrophilic film-shaped polymer matrix, thereby interfering with bacterial survival during storage, the film-shaped polymer matrix comprising lactic acid producing bacteria is preferably laminated with a water-vapor barrier-material. Thereby the survival of the bacterial cells is further increased during storage. Lamination of a film-shaped polymer matrix comprising lactic acid producing bacteria according to the present invention also gives a mechanically stronger film, that can stand harsher treatment during transport and storage. The film-shaped polymer matrix comprising lactic acid producing bacteria can be laminated on one or both sides. In case the film-shaped polymer matrix is laminated on both sides, the same laminate composition does not have to be used on both sides. Examples of materials suitable to use for a laminate include, but are not limited to, waxes, wax paper, aluminum foil, polyethylene films, ethylene copolymer, and coextruded films, such as Suranex (Dow Chemical Company, Reinmuenster, Germany). The lamination could be performed using coextrusion technique or by running the film-shaped polymer matrices through rolling, slightly heated cylinders. Optionally weak adhesives could be used to bond the film-shaped polymer matrix and laminate together. Ultrasonic techniques could also be used for bonding the film-shaped polymer matrix comprising lactic acid producing bacteria and laminate together. Suitable laminate materials have a water vapor transmission rate of, measured according to ASTME 398-83 at 37.8 °C (100°F) and 90% relative humidity 10 g/m2/24 h or below, more preferably 5 g/m 2 /24 h or below, most preferably 2 g/m 2 /24 h or below. The water-vapor barrier-material is to be removed before use of the product comprising the film-shaped polymer matrix.
WO 2004/105822 PCT/SE2004/000806 8 The manufacturing processes for preparing film-shaped polymer matrixes comprising lactic acid producing bacteria according to the present invention involves preparing an aqueous solution of polymer(s) by dissolving the polymer(s) in water, dispersing the bacteria in the aqueous solution of polymer(s) and subsequently drying the dispersion of dissolved polymer and lactic acid producing bacteria on an inert surface at a temperature below 50°C. Optionally, additional components can be added to the polymer solution, either before or after dispersion of the bacteria in the aqueous solution of polymer(s). The aqueous solution of polymer can comprise one or more polymers. The polymer solution comprising the lactic acid producing bacteria is typically casted onto an inert surface, which can be a laminate material or other inert surface, such as the surface of a hygiene product, using a doctor's blade set to a predetermined width. The casted film-shaped polymer matrices are solidified as the solvent is rapidly evaporated. The evaporation could take place in ambient air, an oven, on a heated roll, by convective drying means or on a surface exposed to IRradiation. Preferred temperature intervals during evaporation when heated roll or convective drying methods are used are 5-50°C, more preferably 20-40 0 C and most preferably 30-37°C. When IR-radiation is used for drying somewhat higher temperatures can be reached without detrimental effects on bacterial survival, due to the short drying times that are required. Typically, drying times in the order of seconds to minutes and drying temperatures up to 65°C could be used for IR-drying. Thereafter, optionally, the film-shaped polymer matrix comprising lactic acid producing bacteria can be laminated as described above.
The specific growth conditions, harvest conditions and suitable additional components for the lactic acid producing bacteria has to be optimized for each specific strain in order to ensure a high survival of the bacteria during manufacturing and storage of the film-shaped polymer matrix. The skilled person is familiar with such optimizations. The lactic acid producing bacteria added to the polymer solution can either be freshly prepared or a frozen or dried preparation. Preferably freshly prepared bacterial preparations are used for the present invention.
WO 2004/105822 PCT/SE2004/000806 9 The dispersion of polymer and lactic acid producing bacteria which can be used for producing a film-shaped polymer matrix according to the present invention could also be directly casted or sprayed onto a material, or a material can be dipped in the dispersion of polymer and lactic acid producing bacteria, which after drying is a part of a product, such as a hygiene product.
There are other processes available for the formation of film-shaped polymer matrixes comprising lactic acid producing bacteria, i.e. calendering and extrusions that can be used as long as they are operating at temperatures not harmful for the bacteria.
When starch is used for manufacturing of the film-shaped polymer matrix the starch has to be gelatinized before use. One way this might be performed is by heating a suspension of the starch (grains or granules) in water at a temperature of approximately 90-100°C for 30-60 min. Before addition of the bacteria to the gelatinized starch, the suspension is cooled to 37C or less.
The film-shaped polymer matrix comprising lactic acid producing bacteria according to the present invention is preferably added to hygiene products, such as hygiene tissues, incontinence guards, diaper, panty liners, tampons, sanitary napkins etc., in which the film-shaped polymer matrix comprising lactic acid producing bacteria, when exposed to moisture and wet conditions, result in dissolution of the filmshaped polymer matrix and transfer of the lactic acid producing bacteria to the skin and/or the urogenital area.
By hygiene tissue" is meant any device for wiping skin, for instance, a washcloth, patch, towelette, napkin, wetwipe, and the like. The hygiene tissue provided can be composed of a matrix comprising any natural or synthetic fiber, such as rayon, cellulose, regenerated cellulose, polyester, polyolefine fibers, textile and the like, or foam, nonwoven, felt or batting, or combinations thereof. The film-shaped polymer WO 2004/105822 PCT/SE2004/000806 matrix comprising the lactic acid producing bacteria is applied to the hygiene tissue by dipping the tissue in a dispersion of polymer and lactic acid producing bacteria which can be used for producing a film-shaped polymer matrix before drying of the hygiene tissue.
The film-shaped polymer matrix comprising lactic acid producing bacteria according to the present invention, is, as described above, particularly suitable for application to absorbent products, such as sanitary napkins, panty-liners, diapers, tampons, incontinence guards etc, since these products provide a convenient means for delivery of lactic acid producing bacteria to the urogenital area. The absorbent products according to the invention are preferably composed of a liquid permeable casing sheet, a liquid impermeable backing sheet, an absorbent layer, comprised of one or more layers, placed between said upper layer and said back sheet and optionally a device for adherence. The film-shaped polymer matrix according to the present invention is preferably placed on the permeable casing sheet, but can also be placed inside this. Alternatively, the film-shaped polymer matrix comprising lactic acid producing bacteria according to the present invention can be provided separately and placed on the absorbent product by the users before use. The number of probiotic bacteria in an absorbent product according to the present invention is 107-1024 CFU, preferably 108-i1" CFU, most preferablyl0 9 10 0 CFU.
Below a more detailed description of an absorbent product, such as a sanitary napkin, panty liner, diaper or incontinence guard is given. The absorbent product 1 shown in Fig. 1 and Fig. 2 (cross-section of the absorbent product depicted in Fig. 1 along the line II-II in Fig. 1) includes a liquid-permeable casing sheet or top sheet 2 disposed on that side of the absorbent product which is intended to lie proximal to the wearer in use. The liquid-permeable casing sheet 2 will conveniently consist in a somewhat soft, skin-friendly material. Different types of non-woven material are examples of suitable liquid-permeable materials. Other casing sheet materials that WO 2004/105822 PCT/SE2004/000806 11 can be used are perforated plastic films, net, knitted, crocheted or woven textiles, and combinations and laminates of the aforesaid types of material.
The absorbent product 1 also includes a liquid-impermeable casing sheet or backing sheet 3, disposed on that side of the napkin 1 distal from the wearer in use. The liquid-impermeable casing sheet 3 is conventionally comprised of thin plastic film.
Alternatively, there may be used a liquid-permeable material that has been rendered impermeable to liquid in some way or another. For instance, the liquid-permeable material may be coated with a glue that is impermeable to liquid, and the liquidpermeable layer laminated with a liquid-impermeable material, or hot-calendering a material that was initially liquid-permeable, such as to melt down the surface of the material and therewith obtain a liquid-impermeable layer. Alternatively, there may be used other textiles comprised of hydrophobic fibers and so impervious as to enable them to be used as a liquid barrier layer. The liquid-impermeable casing sheet 3 may beneficially be vapor permeable.
The two casing sheets 2, 3 form a joining edge 4 that projects outwardly around the napkin contour line, and are mutually joined at this edge. The sheets may be joined together by means of any appropriate conventional technique, such as gluing, welding or sewing.
The absorption core 5 sandwiched between the casing sheets 2, 3 shall constitute the layer capable of receiving and storing essentially all liquid discharged by the wearer.
The absorption core 5 may, for instance, be produced from cellulose pulp. This pulp may exist in rolls, bales or sheets that are dry-defibred and converted in a fluffed state to a pulp mat, sometimes with an admixture of superabsorbents, which are polymers capable of absorbing several times their own weight of water or body liquid (fluid). Examples of other usable materials are different types of foamed materials known, for instance, from SE 9903070-2, natural fibers, such as cotton fibers, peat, or the like. It is, of course, also possible to use absorbent synthetic fibers, or WO 2004/105822 PCTiSE2004/000806 12 mixtures of natural fibers and synthetic fibers. Patent Application SE 9903070-2 describes a compressed foam material of regenerated cellulose, e.g. viscose. Such foam material will preferably have a density of 0.1 to 2.0 g/cm 3 The absorbent material may also contain other components, such as foam-stabilizing means, liquiddispersing means, or a binder, such as thermoplastic fibers, for instance, which have been heat-treated to hold short fibers and particles together so as to form a coherent unit.
A fastener means 6 in the form of an elongate rectangular region of self-adhesive is provided on the surface of the liquid-impermeable casing sheet 3 that lies distal from the wearer in use. The fastener means 6 extends over the major part of the liquid-impermeable casing sheet 3. The invention is not restricted to the extension of the fastener means 6, and said means may have the form of elongate stripes, transverse regions, dots, circles, or other patterns and configurations. Neither is the invention restricted to the use of solely adhesive fastener means, since friction fasteners may be used and other types of mechanical fasteners, such as press studs, clips, girdles, pants or the like may be used when found suitable to do so. When an adhesive fastener means is used this is commonly protected, by a protective layer 9, from adhering to other surfaces prior use, which would destroy the fastener means.
The film-shaped polymer matrix comprising lactic acid producing bacteria according to the present invention, when used in an absorbent product, is arranged onto or directly beneath the liquid-permeable casing sheet 2. Preferably the film-shaped polymer matrix comprising lactic acid producing bacteria is placed in such a way that the film-shaped polymer matrix does not cover the entire surface of the absorbent product. In this way the film-shaped polymer matrix comprising lactic acid producing bacteria does not interfere with absorption of bodily fluids (such as blood, urine, secretion etc.) by the absorbent product. Even if the film-shaped polymer matrix comprising lactic acid producing bacteria solublizes by bodily fluids, initial absorption by the absorbent product can be impaired before the film-shaped polymer WO 2004/105822 PCTSE2004/000806 13 matrix is solubilized if the whole of an absorbent product is covered by the filmshaped polymer matrix. On way to solve this is to supply the absorbent article with a film-shaped polymer matrix comprising lactic acid producing bacteria according to the present invention which has been punched, forming at least one opening through which bodily fluids can be transported. More preferably, a carrier in the form of a net or loose non-woven sheet, dipped in a dispersion of polymer and lactic acid producing bacteria which can be used for producing a film-shaped polymer matrix according to the present invention, may also be arranged onto or directly beneath the liquid-pen-rmeable casing sheet. In Fig. 1 and 2 one way to place the film-shaped polymer matrix comprising lactic acid producing bacteria is exemplified, wherein the film-shaped polymer matrix is placed in strings 8.
In a similar manner to what is described above, a tampon comprising the filmshaped polymer matrix can be prepared. Fig. 3 and 4 (cross-section of the tampon in Fig. 3 along the line IV-IV) depict a schematic exemplary drawing of a tampon comprising a film-shaped polymer matrix 13 according to the present invention, wherein the film-shaped polymer matrix 13 is arranged onto the casing sheet 11.
Also depicted is the absorbent core 12.
The skilled person could easily use the above exemplary descriptions of hygiene products comprising a film-shaped polymer matrix according to the present invention to manufacture a hygiene product comprising a film-shaped polymer matrix according to the invention. Therefore, alternative designs of a sanitary napkin, incontinence guard, panty-liner, diaper, tampon, hygiene tissue etc are also included in the present invention.
The embedding of lactic acid producing bacteria in a film-shaped polymer matrix according to the present invention is also suitable for increasing the survival of the bacteria in pharmaceutical preparations and in the food industry.
WO 2004/105822 PCTiSE2004/000806 14 A film-shaped polymer matrix comprising lactic acid producing bacteria according to the present invention have several advantages. When freeze-dried bacteria or suspensions of bacteria are added directly to a hygiene product, the product have to be dried in order for the inherent moisture content in the product not to affect bacterial survival negatively. This is both a complicated process, since the whole product has to be dried, but drying of the hygiene product can also result in a lowered initial absorption of bodily fluids as some residual moisture facilitates initial absorption. In addition, further protection form moisture during transport and storage by water impervious packaging is necessary for maintenance of bacterial survival in this case.
This necessity to provide the whole hygiene product in a moisture impervious packing unit results in higher production costs. In comparison, by embedding the bacteria in a film-shaped polymer matrix according to the present invention one avoids the need for drying the hygiene product and the use of moisture impervious packing units, still having a resulting high survival of the bacterial cells, even after prolonged storage. The use of a film-shaped polymer matrix according to the present invention furthermore alleviates the use of freeze-dried bacterial preparations, which are the mostly common preparation form for bacteria for use in hygiene products, but which are costly and complicated to prepare. Instead suspensions of lactic acid producing bacteria can be used directly when preparing a film-shaped polymer matrix comprising lactic acid producing bacteria which is cheaper and more practical.
A film-shaped polymer matrix comprising lactic acid producing bacteria can also be prefabricated and later placed on many different products, such as hygiene products etc. without any special adaptations for the different products. Also, the production of products comprising bacteria requires special hygiene requirements at the manufacturing plant. Costs can therefore be reduced by producing the film-shaped polymer matrix comprising lactic acid producing bacteria at another location.
Using a film-shaped polymer matrix in order to protect lactic acid producing bacteria from moisture leads to high stability at common temperatures during transport WO 2004/105822 PCT/SE2004/000806 and storage, since the film structure in itself is very insensitive to temperature variations. Also, the dryness of the bacterial cells in the dry film-shaped polymer matrix renders the bacteria more heat tolerant.
Furthermore, the preparation of a film-shaped polymer matrix is more gentle to the lactic acid producing bacteria, compared to for example extrusion or spraying that is often used when the bacteria are mixed with hydrophobic substances.
The use of a film-shaped polymer matrix comprising lactic acid producing bacteria is also advantageous in terms of efficacy of transfer of the bacteria to the skin.
When the film-shaped polymer matrix comprising lactic acid producing bacteria dissolves, fragments of the film-shaped polymer matrix may be transferred to the skin where they dissolve further. Thereby the film fragments act as a vehicle for transfer of the bacteria. Also, the use of a film-shaped polymer matrix ensures that the bacteia are kept in the outer layers of the hygiene product, thereby ensuring a high transfer rate. When the film-shaped polymer matrix comprising lactic acid producing bacteria is applied to a hygiene product, transfer rates can also be optimized by the choice of commonly used surface materials.
The present invention therefore solves many of the problems associated with providing products comprising lactic acid producing bacteria. Below the present invention is further described by illustrative but non-limiting examples.
Example 1 Production of a film-shaped polymer matrix comprising lactic acid producing bacteria An aqueous polymer solution with a concentration of 0.1-10 by weight is prepared by dissolving polyethylene oxide, polyvinyl pyrrolidone, polyvinyl alcohol or starch in water.
WO 2004/105822 PCT/SE2004/000806 16 One part of bacterial suspension (ca 1010 CFU/ml, BioNativ AB, Box 7979, 907 19 Umeh, Sweden) comprising Lactobacillus plantarum 931 (deposition No. (DSMZ): 11918) is mixed with 9 parts of the polymer solution for 5 minutes. The mixture is poured into small petri-dishes in a quantity that ensures the right thickness and an amount of bacteria of approximately 109 cfu/film-shaped polymer matrix. The resulting film-shaped polymer matrix comprising lactic acid producing bacteria has a thickness of preferably 50 pm-5 mm, more preferably 100 gim-1 mm and most preferably 500 pm-1 mm.
The petri dish is placed in a climate chamber at a temperature of 37"C and with as low relative humidity as possible (10% or below), whereby the water evaporates and the film-shaped polymer matrix solidifies and the bacteria are immobilized in the film-shaped polymer matrix.
The water activity of the film-shaped polymer matrixes comprising lactic acid producing bacteria is measured using an aw-instrument; DD401102 Aqualab Serie 3TE (ADAB Analytical Devices AB, Stockholm, Sweden).
Within 1-2 weeks after production of the film-shaped polymer matrix transfer tests from absorbent products comprising the film-shaped polymer matrix according to the present invention to skin are performed (see below).
Example 2 Survival ofL. plantarum 931 in film-shaped polymer matrices according to the present invention The film-shaped polymer matrix is placed in a climate chamber at 23 °C and relative humidity. The survival of the bacteria is tested in a film-shaped polymer matrix according Example 1 furthermore coated with Caremelt (a mixture of waxes, Cognis, Henkel KgaA, Dusseldorf, Germany). The survival of the bacteria in the WO 2004/105822 PCT/SE2004/000806 17 film-shaped polymer matrix is tested at predetermined intervals during several months (see below).
To test the survival of the lactic acid producing bacteria in the film-shaped polymer matrix, the film is placed in a petri-dish, immersed with 20 ml ofNaCI and put on a shaking-device. After 40 minutes the film-shaped polymer matrix is dissolved and the survival of the bacteria is determined by counting the number of colony forming units (CFU) by standard spread-plate techniques and cultivation on MRS agar (2 days of incubation at 37°C). The results are presented in Fig. wherein PVP is polyvinyl pyrrolidone.
Example 3 Transfer of L. plantarum 931 from a pantly-liner provided with a film-shaped polymer matrix Film-shaped polymer matrixes comprising lactic acid producing bacteria are produced as described in Example 1. About 1 cm 2 of film-shaped polymer matrix are cut out, weighted and placed on the nonwoven top-layer of a panty-liner specimen (a circle 2.5 cm in diameter, punched out of an absorbent product).
100 ul ofNaCI are added to the absorbent product, comprising the film-shaped polymer matrix with bacteria, with a pipette, and the specimen is subsequently mounted, with constant pressure (elastic tape, and elastic bandage), on to the forearm of volunteers. After 2 hours the product is removed and the number of transferred Lactobacilli on the skin measured. A sterile stainless-steel cylinder (2.6 cm in diameter, height 2 cm) is held tight to the skin (that has been covered with the specimen), and 1 ml of phosphate buffer (0.1M, pH 7.2) is poured into the cylinder.
With a smooth glass-stick the skin is gently "kneaded" for 1 minute. Afterwards, the buffer is collected with a pipette and CFU measured with spread- plate technique and MRS agar.
The percentage of transferred Lactobacilli is calculated by dividing the number of CFU collected from the skin area covered by the specimen with the total number of CFU in the film-shaped polymer matrix on the test specimen. The number of Lactobacilli initially present on the skin at the sample site is very low, especially in respect of the number of L.
plantarum 931 transferred to the skin. Therefore the number of Lactobacilli detected on the skin after the transfer test are considered to be a result of transfer from the specimen comprising L. plantarum 931. As a comparison, a panty liner with a dry bacterial preparation not embedded in a film-shaped polymer matrix was used. As can be seen in Table 1 the percentage of bacteria transferred to the skin from an absorbent product was enhanced using a film-shaped polymer matrix according to the present invention.
Table 1 Initial No. of Cone. MW No. of CFU CFU on Transferred Polymer (kDa) transferred to the skin product PVOH 1 96 3.E+04 1.E+06 2.4 PVP 1 40 7.E+07 6.E+08 12.7 Comparison 2.E+05 4.E+07 0.
PVP Polyvinyl pyrrolidone PVOH Polyvinyl alcohol Comprises/comprising and grammatical variations thereof when used in this specification are to be taken to specify the presence of stated features, integers, steps or components or groups thereof, but do not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof.
Claims (14)
1. A single layer polymer matrix film comprising lactic acid producing bacteria in a pharmaceutically acceptable polymer(s), which phannaceutically acceptable polymer(s) is able to protect bacterial cells from moisture during storage, but which can be dissolved in bodily fluids.
2. A single layer polymer matrix film according to claim 1, wherein the lactic acid producing bacterial strain(s) is isolated from the skin or urogenital area of a healthy person.
3. A single layer polymer matrix film according to claim 1 or 2 wherein the lactic acid producing bacterial strain is selected from the genera Pediococcus, Lactococcus, Lactobacillus or mixes thereof.
4. A single layer polymer matrix film according to claim 3 wherein the lactic acid producing bacterial strain is selected from at least Lactobacillus plantarum.
A single layer polymer matrix film according to claim 4 wherein the lactic acid producing bacterial strain is selected from at least Lactobacillus plantarum 931 (deposition No. (DSMZ): 11918).
6. A single layer polymer matrix film according to claims 1-5 wherein the polymer(s) is chosen from the group comprising polysaccharides and derivatives thereof and synthetic hydrophilic polymers and derivatives thereof.
7. A single layer polymer matrix film according to claims 1-6, wherein the polymer(s) is chosen from the group comprising polyvinyl alcohol, polyethyleneoxide, polyvinyl pyrrolidone, and starch.
8. A single layer polymer matrix film according to claims 1-7 further comprising additional components.
9. A single layer polymer matrix film according to claims 1-8 which single layer polymer matrix film has a thickness of preferably 50 gm-5 mm, more preferably 100 gm-1 mm and most preferably 500 utm-1 mm.
A single layer polymer matrix film according to claims 1-9 wherein the water activity of the single layer polymer matrix film is 0.30 or below, preferably 0.25 or below and most preferably 0.20 or below.
11. A single layer polymer matrix film according to claims 1-10 wherein the bacterial concentration is 107 -1014 CFU/g film, more preferably 10'-1012 CFU /g film.
12. A single layer polymer matrix film according to claims 1-11 which single layer polymer matrix film further comprises a laminate layer placed on at least one side of the single layer polymer matrix film.
13. A hygiene product, such as a sanitary napkin, panty liner, diaper, incontinence guard, tampon, hygiene tissue, comprising a single layer polymer matrix film comprising lactic acid producing bacteria according to claims 1-12.
14. A process for producing a single layer polymer matrix film according to claims 1- 11 comprising lactic acid producing bacteria comprising the steps of: a) preparing an aqueous solution of polymer that is non-toxic and non-irritant to a user's skin and mucous membranes b) dispersing lactic acid producing bacteria in said solution of polymer c) optionally adding an additional component to the dispersion d) drying said resulting dispersion comprising lactic acid producing bacteria on a inert surface, at a temperature below 50'C, thereby producing a single layer polymer matrix film e) optionally laminating the resulting single layer polymer matrix film; wherein steps b) and c) can be performed in any order. A product comprising a) a hygiene product, such as a sanitary napkin, panty liner, diaper, incontinence guard, tampon, hygiene tissue; and b) a single layer polymer matrix film according to claims 1-12. SCA HYGIENE PRODUCTS AB WATERMARK PATENT TRADE MARK ATTORNEYS P26280AU00
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0301552-6 | 2003-05-27 | ||
SE0301552A SE526982C2 (en) | 2003-05-27 | 2003-05-27 | One-layer polymer matrix film comprising lactic acid producing bacteria, preparation and use thereof |
PCT/SE2004/000806 WO2004105822A1 (en) | 2003-05-27 | 2004-05-26 | New product |
Publications (2)
Publication Number | Publication Date |
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AU2004243133A1 AU2004243133A1 (en) | 2004-12-09 |
AU2004243133B2 true AU2004243133B2 (en) | 2009-02-26 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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AU2004243133A Ceased AU2004243133B2 (en) | 2003-05-27 | 2004-05-26 | New product |
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EP (1) | EP1651284A1 (en) |
JP (1) | JP2007502900A (en) |
CN (1) | CN100382851C (en) |
AU (1) | AU2004243133B2 (en) |
BR (1) | BRPI0410738A (en) |
CA (1) | CA2523977A1 (en) |
MX (1) | MXPA05011548A (en) |
RU (1) | RU2352627C2 (en) |
SE (1) | SE526982C2 (en) |
WO (1) | WO2004105822A1 (en) |
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US8124016B2 (en) | 2005-11-15 | 2012-02-28 | Lg Electronics, Inc. | Articles with antimicrobial property and manufacturing method thereof |
BRPI0520778A2 (en) * | 2005-12-20 | 2009-05-26 | Sca Hygiene Prod Ab | absorbent article |
JP2009521253A (en) * | 2005-12-22 | 2009-06-04 | エスセーアー・ハイジーン・プロダクツ・アーベー | Water vapor impermeable carrier member used in absorbent articles |
JP4800393B2 (en) * | 2005-12-22 | 2011-10-26 | エスセーアー・ハイジーン・プロダクツ・アーベー | Absorbent article |
EP2026736B1 (en) | 2006-05-04 | 2014-05-28 | Sca Hygiene Products Ab | Multi function wrapper |
AU2006350773A1 (en) * | 2006-11-17 | 2008-05-22 | Sca Hygiene Products Ab | Sanitary article comprising a microbe-inhibiting composition |
EP2155272A4 (en) * | 2007-06-21 | 2012-06-27 | Sca Hygiene Prod Ab | Sanitary article comprising lactobacilli in a hydrophilic carrier |
CN102120045A (en) * | 2011-03-02 | 2011-07-13 | 润盈生物工程(上海)有限公司 | Probiotics-containing female hygienic product as well as preparation method and application thereof |
US20120259166A1 (en) * | 2011-04-11 | 2012-10-11 | Nancy Karapasha | Array of pessary devices |
NL2007037C2 (en) * | 2011-07-04 | 2013-01-07 | Csk Food Enrichment Bv | Cheese coating compositions having biopreservative properties. |
US20140179615A1 (en) * | 2012-07-12 | 2014-06-26 | Hainan Weikang Pharmaceutical (Qianshan) Co., Ltd. | Glutathione and Acetaminophen Composition and Preparation Method Thereof |
EP3097183A1 (en) * | 2014-01-24 | 2016-11-30 | The Procter & Gamble Company | Web comprising a microorganism-containing fibrous element and method for making same |
US20150209392A1 (en) * | 2014-01-24 | 2015-07-30 | The Procter & Gamble Company | Filaments Comprising a Microorganism and Method for Making Same |
US20150209468A1 (en) * | 2014-01-24 | 2015-07-30 | The Procter & Gamble Company | Hygiene article containing microorganism |
US20170020750A1 (en) * | 2015-07-23 | 2017-01-26 | The Procter & Gamble Company | Patch containing microorganism |
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2004
- 2004-05-26 AU AU2004243133A patent/AU2004243133B2/en not_active Ceased
- 2004-05-26 BR BRPI0410738-1A patent/BRPI0410738A/en not_active IP Right Cessation
- 2004-05-26 CN CNB2004800143758A patent/CN100382851C/en not_active Expired - Fee Related
- 2004-05-26 CA CA002523977A patent/CA2523977A1/en not_active Abandoned
- 2004-05-26 MX MXPA05011548A patent/MXPA05011548A/en not_active Application Discontinuation
- 2004-05-26 RU RU2005136871/13A patent/RU2352627C2/en not_active IP Right Cessation
- 2004-05-26 EP EP04734963A patent/EP1651284A1/en not_active Withdrawn
- 2004-05-26 WO PCT/SE2004/000806 patent/WO2004105822A1/en active Search and Examination
- 2004-05-26 JP JP2006532211A patent/JP2007502900A/en active Pending
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US20030003138A1 (en) * | 2000-01-18 | 2003-01-02 | Achille Di Cintio | Articles with spores exhibiting antagonistic properties against pathogens and/or spores forming micro-organisms |
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Also Published As
Publication number | Publication date |
---|---|
RU2352627C2 (en) | 2009-04-20 |
SE0301552D0 (en) | 2003-05-27 |
CN100382851C (en) | 2008-04-23 |
BRPI0410738A (en) | 2006-06-27 |
SE0301552L (en) | 2004-11-28 |
CA2523977A1 (en) | 2004-12-09 |
AU2004243133A1 (en) | 2004-12-09 |
RU2005136871A (en) | 2006-06-10 |
WO2004105822A1 (en) | 2004-12-09 |
MXPA05011548A (en) | 2005-12-15 |
EP1651284A1 (en) | 2006-05-03 |
SE526982C2 (en) | 2005-11-29 |
JP2007502900A (en) | 2007-02-15 |
CN1795018A (en) | 2006-06-28 |
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