CN100382851C - New product - Google Patents

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CN100382851C
CN100382851C CNB2004800143758A CN200480014375A CN100382851C CN 100382851 C CN100382851 C CN 100382851C CN B2004800143758 A CNB2004800143758 A CN B2004800143758A CN 200480014375 A CN200480014375 A CN 200480014375A CN 100382851 C CN100382851 C CN 100382851C
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base film
polymer layer
layer base
single polymer
film
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CN1795018A (en
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U·胡斯马克
I·古斯塔弗森
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Essity Hygiene and Health AB
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SCA Hygiene Products AB
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/36—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing microorganisms
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
    • C08J5/18—Manufacture of films or sheets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Manufacturing & Machinery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Materials Engineering (AREA)
  • Hematology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Absorbent Articles And Supports Therefor (AREA)
  • Laminated Bodies (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Orthopedics, Nursing, And Contraception (AREA)
  • Compositions Of Macromolecular Compounds (AREA)

Abstract

The present invention relates to film-shaped polymer matrixes comprising lactic acid producing bacteria that are dissolved when exposed to wet conditions. The film-shaped polymer matrixes according to the present invention protect bacterial cells from moisture thereby increasing bacterial survival during transport and stor-age. The film-shaped polymer matrix also results in a high transfer of bacterial cells to the skin of a subject.

Description

New product
Technical field
The present invention relates to a kind of film-shaped polymer matrix that contains the antibacterial of lactic acid producing, this film-shaped matrix is made up of skin and the nontoxic also non-irritating polymer of mucosa to user.The product that the invention still further relates to the preparation method of this film-shaped polymer matrix and contain this film-shaped polymer matrix.
Background technology
Urogenital has been held the microbial ecosystem of a complexity, comprising bacterial species different more than 50 kinds (Hill et al., Scand.J.Urol.Nephrol.1984; 86 (suppl.) 23-29).In women's urogenital breeding at reproduction age mainly is the Lactobacillus antibacterial of lactic acid producing.The antibacterial of these lactic acid producing is very important for the flora that keeps this zone health, and as probiotic bacteria pathogen is had antagonism.The antibacterial of lactic acid producing forms biomembrane by capturing the subenvironment of suitable invasion, competes available nutrient substance, has repelled the harmful microbe invasion thus, thereby has suppressed other microbial growth and invasion.Equally, the hydrogen peroxide enzyme product, special effect inhibitor can suppress the invasion of other microorganism as bacteriocin and the generation that can reduce the organic acid (comprising lactic acid and acetic acid) of pH value.
During hormone changed, the microbial ecosystem of healthy individual can be because of using the antibiotic multilated, as conceived or use estrogen contraceptive during, menstrual phase, climacteric, diabetics etc.Microorganism also can propagate into urogenital from anus, thereby causes infection.This will cause the normal flora imbalance and cause the individual susceptible to bacterial infection, thereby causes vaginitis, urinary tract infection and common skin infections.Usually infect relevant microorganism with this class and belong to Escherichia, Enterococcus, Rhodopseudomonas, Proteus, Klebsiella, Streptococcus, staphylococcus, Gardner Bordetella and candida mycoderma Pseudomonas.Because anus and urethra close together, the women has born special risk, especially young woman and old women, and the former does not reach full growth by the flora of urogenital as yet, and the latter then no longer includes the flora that shields.
An approach that reduces above-mentioned infection problems is that a good Personal hygiene is arranged.Yet, excessively use cleaning agent not only to reduce the quantity of harmful bacterium, and can damage profitable strain, thereby make the pathogen invasion cause infecting once more.Can be chosen in the antibacterial that gives lactic acid producing on urogenital and the skin, vie each other with pathogen, and help to recover and keep this regional profitable strain, this is treatment that has been found that and the effective ways that prevent bacterial infection.
Prove that now the antibacterial of lactic acid producing can be transported by absorbing product, as diaper, sanitary towel, incontinence protection, panty-liners and catamenial tampons, for example WO92/13577, WO97/02846, WO 99/17813, described in WO 99/45099 and the WO 00/35502.
The subject matter that product ran into that preparation is used to transport the antibacterial of lactic acid producing is that these antibacterials must keep viability in product transhipment and storage process.The antibacterial of lactic acid producing can be lost survival ability rapidly under the condition of humidity, therefore, it is very important that antibacterial can not be exposed to wet environment.In the absorption product of the antibacterial that contains lactic acid producing, a kind of method that part overcomes this problem is to add antibacterial in product, thereby dry described product is removed most of dampness wherein, and product is placed the packing (WO 99/17813) of sealed damp-proof.
Prevent that the optional method that antibacterial makes moist from being that antibacterial is dispersed in the lyophobic dust (referring to US 4,518,696; WO 92/13577; WO 02/28446) and since the hydrophobic property of this material it will prevent that moisture from arriving the bacterial cell of institute's embedding.
Yet, still need to develop optional method that the antibacterial that prevents lactic acid producing makes moist and make it be suitable for antibacterial being applied to experimenter and can long preservation and bacterial cell can not lost viability, and the antibacterial of lactic acid producing can be transitted to user effectively by expection.In addition, still needing to develop preparation technology makes it efficient more and reduce cost.
Summary of the invention
The inventor is surprised has found the optional method that a kind of bacterial cell that prevents lactic acid producing makes moist, thereby has improved the survival rate of antibacterial in transportation and storage process.Solution of the present invention also can effectively be transported to bacterial cell on experimenter's the skin.According to the present invention, bacterial cell is embedded in the film-shaped polymer matrix, improves the survival rate of antibacterial thereby this film-shaped polymer matrix can prevent that antibacterial from making moist.The invention still further relates to preparation method of film-shaped polymer matrix of this antibacterial that contains lactic acid producing and the product that contains this film-shaped polymer matrix.
Description of drawings
Fig. 1 has described a kind of exemplary embodiment that absorbs product, as sanitary towel, and diaper, panty-liners, incontinence protection and other analog that contains film-shaped polymer matrix of the present invention.
Fig. 2 has shown absorption product shown in Figure 1 cross section along II-II line among Fig. 1.
Fig. 3 has described a kind of sketch map that contains the catamenial tampons of film-shaped polymer matrix of the present invention.
Fig. 4 has shown absorption product shown in Figure 3 cross section along IV-IV line among Fig. 3.
Fig. 5 has shown the survival rate of film-shaped polymer matrix of the present invention through the bacterial cell of lactic acid producing after the long term storage.
Detailed Description Of The Invention
The product that the present invention relates to contain the bacterium of lactic acid producing keeps the problem of bacteria living power and the problem that bacterium is transported to satisfactorily the user from product from be prepared into use procedure. In storage process, the most important factor that improves Survival probability of bacteria is to prevent that bacterium from making moist. The inventor finds in surprise, the bacterium of lactic acid producing is embedded in the film-shaped polymer matrix can greatly improves the survival rate of bacterium cell in storage process.
The polymer that is fit to film-shaped polymer matrix can prevent that bacterial cell from making moist in storage process, but it can be dissolved in the body fluid, in the time of therefore in being exposed to wet or moist environment, can discharge bacterial cell.The film-shaped polymer matrix that contains the antibacterial of lactic acid producing of the present invention is made up of user skin and mucosa bacterial isolates nontoxic and non-irritating polymer and at least a lactic acid producing at least a.Be fit to polymer of the present invention and comprise natural hydrophilic polymer, as polysaccharide and derivant thereof, as starch and cellulose (derivant that comprises them), protein, hydrophilic polymer is as synthetic hydrophilic polymer, as acrylate copolymer, polyethers, as poly(ethylene oxide), polyurethane, polyamide, polyacrylonitrile, polyvinyl is as polyvinylpyrrolidone and polyvinyl alcohol or the like.The preferred polymer of the present invention includes, but not limited to polyvinyl alcohol, poly(ethylene oxide), polyvinylpyrrolidone and starch.These polymer have constituted the germy film-shaped matrix of embedding, thereby prevent that antibacterial from making moist.These polymer can use separately or with different combining forms.
The film-shaped polymer matrix that contains the antibacterial of lactic acid producing of the present invention also can comprise supplementary element.The example of these compositions includes, but not limited to the antibacterial protective agent in the polymeric film preparation process; as carbohydrate, as maltose, sucrose; trehalose, lactose, glucose and fructose; protein is as skimmed milk and albumin, aminoacid; as sodium glutamate, polyhydric alcohol is as xylitol; mannitol and sorbitol, and antioxidant are as sodium ascorbate.In case polymeric film is dissolved, these material majorities can serve as vegetative nutrient.Supplementary element can be the plasticizer that can be added in the polymeric film that comprises starch also, as polyhydric alcohol, as glycerol, polyhydric alcohol, sorbitol and polyvinyl alcohol, sucrose ester, as sucrose stearate, fatty acid is as Palmic acid, based on the lipid of esterified fatty acid, as monoglyceride, diglyceride and triglyceride, wax and amino alcohol, as triethanolamine and N-methyl-diethanolamine, and enzyme, as amylase.
The concentration of polymer solution is preferably 0.1-10 (w/w) %, and more preferably 0.5-7 (w/w) % most preferably is 1-2 (w/w) %.The thickness of film-shaped polymer matrix that contains the antibacterial of lactic acid producing is preferably 50 μ m-5mm, and more preferably 100 μ m-1mm most preferably are 500 μ m-1mm.
In temperature is 23 ℃, and relative humidity is at least three months storing processs of 50%, in order to increase the survival rate of antibacterial, the water activity of film-shaped polymer matrix that contains the antibacterial of lactic acid producing be 0.30 or below, be preferably 0.25 or below, most preferably be 0.20 or below.
The number of bacteria of the lactic acid producing in the film-shaped polymer matrix of the present invention is preferably 10 7-10 14The CFU/g film, more preferably 10 9-10 12The CFU/g film.
In order to make film-shaped polymer matrix transfer of bacteria more fully, in the time of in being exposed to humidity or wet environment, film-shaped polymer matrix is dissolving as far as possible apace preferably, in either case, its within 6 hours, preferably dissolving within 4 hours.
The present invention selects for use the antibacterial of lactic acid producing to prevent owing to their and treats in the urogenital and the good effect of the infected by microbes aspect on the skin.This antibacterial preferable separate is from the natural flora of healthy human body, and preferred bacterium separates from skin or urogenital.Preferred " antibacterial of lactic acid producing " that be suitable for the object of the invention comprises and is selected from Lactobacillus, the antibacterial of Lactococcus and Pediococcus.Preferably selected antibacterial is from lactococcus lactis, bacillus acidophilus, lactobacillus curvatus or Lactobacillus plantarum.More preferably bacterial isolates is selected from Lactobacillus plantarum.Even more preferably the antibacterial of lactic acid producing is selected from Lactobacillus plantarum 931 (preserving number (DSMZ): 11918).The antibacterial of these lactic acid producing can be used separately or with the form of mixtures that contains two kinds of bacterial isolateses at least.
For preventing that water vapour from infiltrating in the hydrophilic film-shaped polymer matrix, thereby hindered the survival of antibacterial in the storing process, the film-shaped polymer matrix that contains the antibacterial of lactic acid producing preferably uses the steam-preventing material stacked.Can further increase the survival rate of bacterial cell in the storing process like this.The present invention contains the mechanical strength that the stepped construction of film-shaped polymer matrix of the antibacterial of lactic acid producing also can reinforcing membrane, can withstand the treatment of the harshness in transportation and the storage process like this.Containing the film-shaped polymer matrix of the antibacterial of lactic acid producing can be one-sided or bilateral is stacked.If the film-shaped polymer matrix bilateral is stacked, then bilateral needn't use identical stacked compositions.The example that is suitable as the material of duplexer includes, but not limited to wax, paraffin paper, aluminium foil, polyethylene film, ethylene copolymer, and laminated film, as Suranex ((Dow Chemical Company, Reinmuenster, Germany).Stackedly can utilize coextrusion technology to finish or make film-shaped polymer matrix pass rolling, Jia Re cylinder a little.Can randomly use weak binding agent that film-shaped polymer matrix and duplexer are bonded together.Ultrasonic technology also can be used to film-shaped polymer matrix of the antibacterial that contains lactic acid producing and duplexer are bonded together.At 37.8 ℃ (100 °F), relative humidity (RH) is to measure under 90% the condition according to ASTME 398-83, and the water vapour permeability of the stacking material that is fit to is 10g/m 2/ 24h or following, more preferably 5g/m 2/ 24h or following most preferably is 2g/m 2/ 24h or following.The steam-preventing material will be removed before the product that contains film-shaped polymer matrix uses.
The preparation method of film-shaped polymer matrix that the present invention contains the antibacterial of lactic acid producing comprises, by polymer dissolution is prepared the aqueous solution of polymer in water, antibacterial is dispersed in the aqueous solution of polymer, below 50 ℃, drying is dissolved with the dispersion of the antibacterial of polymer and lactic acid producing on the inactive surfaces subsequently.Antibacterial is dispersed in before or after the aqueous solutions of polymers, can optionally add supplementary element in polymer solution.Can comprise one or more polymer in the aqueous solutions of polymers.Usually the polymer solution that uses the scraper that preestablishes width will contain the antibacterial of lactic acid producing is cast on the inactive surfaces, and this inactive surfaces can be stacking material or other inactive surfaces, as the surface of health product.Along with solvent promptly evaporates, the film-shaped polymer matrix after the cast is solidified.Evaporation can occur in the surrounding air, in the baking oven, on the roller of heating, is undertaken by convective drying or method that the surface is exposed in the infrared ray radiation.When using warm-up mill or convective drying method, the temperature interval of evaporation process is preferably 5-50 ℃, more preferably 20-40 ℃, most preferably is 30-37 ℃.When using infrared ray radiation dry, can reach slightly high temperature and can not produce injurious effects the survival of antibacterial, this be because its required drying time very of short duration.Typically, the drying time of infrared drying approximately from the several seconds by several minutes, baking temperature can be up to 65 ℃.Thereafter, randomly, the film-shaped polymer matrix that contains the antibacterial of lactic acid producing can be stacked as mentioned above.
In order to guarantee the high viability of antibacterial in film-shaped polymer matrix preparation and the storage process, for each specific bacterial strain, it is best that specific growth conditions, results condition and suitable supplementary element all will reach.The technical staff is familiar with these optimum conditions very much.The antibacterial that joins the lactic acid producing in the polymer solution can be a prepared fresh, or freezing or exsiccant preparation.The present invention preferably uses the bacteria preparation of prepared fresh.
The dispersion that can be used in the antibacterial of preparation polymer of film-shaped polymer matrix of the present invention and lactic acid producing also can directly be poured into a mould or be sprayed on the material, or material is immersed in the dispersion of antibacterial of polymer and lactic acid producing, it just becomes the part of product dry back, as health product.
Other method also can be used for preparing the film-shaped polymer matrix of the antibacterial that contains lactic acid producing, that is, calendering and extrusion molding all can use, as long as their operative temperature can not damage antibacterial.
When starch was used to prepare film-shaped polymer matrix, starch must be made into glue before using.A kind of method of finishing this step was at approximately 90-100 ℃, with starch (granule or particulate) water slurry heating 30-60 minute.Before joining antibacterial in the gelatinized starch, suspension is cooled to 37 ℃ or lower.
The film-shaped polymer matrix that contains the antibacterial of lactic acid producing of the present invention preferably is added in the health product, as toilet paper, incontinence protection, diaper, panty-liners, catamenial tampons, sanitary towel etc., wherein when this film-shaped polymer matrix that contains the antibacterial of lactic acid producing is exposed in the moist and wet environment, film-shaped polymer matrix will be dissolved and the antibacterial of lactic acid producing is transported to skin and/or urogenital.
" toilet paper " is meant the apparatus of any wiping skin, as face-cloth, and cloth waste, towelette, medicated napkin, wet tissue or the like.Used toilet paper can be formed by containing any matrix natural or synthetic fibers, as artificial silk, and cellulose, regenerated cellulose, polyester, polyolefine fiber, textile etc., or foam, non-woven textile, felt or cotton-wool, or their combination.In the dispersion of antibacterial by toilet paper being immersed in polymer and lactic acid producing, can before the toilet paper drying, prepare film-shaped polymer matrix, thereby the film-shaped polymer matrix of the antibacterial that contains lactic acid producing is coated on the toilet paper.
As mentioned above, the film-shaped polymer matrix that contains the antibacterial of lactic acid producing of the present invention especially is fit to be applied in the absorption product, as sanitary towel, panty-liners, diaper, catamenial tampons, incontinence protection etc. are because these products provide shortcut for the antibacterial of lactic acid producing is transported to urogenital.Absorption product of the present invention preferably by the cover sheet of energy sepage, be made up of one or more layers absorbed layer formed between described top layer and described backing sheet and optional adhesion elements by the backing sheet of liquid-impermeable.Film-shaped polymer matrix of the present invention preferably is placed on the sepage cover sheet, also can be placed on this cover sheet the inside.In addition, the film-shaped polymer matrix that contains the antibacterial of lactic acid producing of the present invention can provide separately and is placed on before use on the absorption product by user.The quantity of the probiotic bacteria in the absorption product of the present invention is 10 7-10 14CFU, preferred 10 8-10 11CFU, most preferably 10 9-10 10CFU.
Below more detailed description is absorbed product, as sanitary towel, panty-liners, diaper, incontinence protection.Shown absorption product 1 comprises and absorbs product and wearer when using near the sepage cover sheet or the top layer sheet 2 of a side among Fig. 1 and Fig. 2 (the absorption product shown in Fig. 1 is along the cross section of II-II line).Sepage cover sheet 2 preferably by softish a little, do not make by the material of chafe.The sepage material that dissimilar non-woven materials is suitable just.Other cover sheet material can be used perforated plastic film, net, fabric, crochet thing or woven fabric, and the combination of the above-mentioned type material or duplexer.
Absorb product 1 and also comprise when using in the sanitary towel 1 liquid-impermeable cover sheet or backing sheet 3 away from wearer's one side.Liquid-impermeable cover sheet 3 contains plastic sheeting usually.As selection, can use to a certain extent the sepage material of liquid-impermeable.For example, can be coated with the glue of last layer liquid-impermeable on the sepage material, and seepage layer and liquid-impermeable material is compressed together; Or the material of a kind of initial sepage of hot pressing, so that the surface of molten material, and then obtain the liquid-impermeable layer.As selection, can use other textile to comprise hydrophobic fibre, its good airproof performance is to being used as liquid barrier layer.Liquid-impermeable cover sheet 3 is the permeate water steam advantageously.
Two cover sheets 2,3 form adjoining edge 4, and this edge has formed the appearance profile line of sanitary towel, and cover sheet 2,3 forms at this edge and interconnects.These two cover sheets can couple together by any suitable routine techniques, as gluing, and welding, sewing.
Be clipped in the absorbent core 5 of cover between the sheet 2,3 and should constitute the layer of all liq basically that can hold and store wearer's discharge.For example, absorbent core 5 can be made by cellulose pulp.This paper pulp exists with forms such as volume, bundle or sheets, and they all are transformed into pulp mat through the super-dry defiber and from loose condition (of surface), mixes sometimes with superabsorbents, and these absorbent are to absorb the water of himself weight several times or the polymer of body fluid (liquid).Other available material can be various known foamed materialss, the natural fiber described in SE 9903070-2, and as cotton fiber, peat or the like.Certainly, also can use absorbent synthetic, or natural and mixture synthetic fibers.A kind of regenerated cellulose foamed materials through overcompression has been described, as viscose among the patent application SE 9903070-2.The preferred 0.1-2.0g/cm of the density of this foamed materials 3Absorbing material also can comprise other component, as foam stabiliser, and liquid dispersant, or binding agent, as thermoplastic fibre, for example, thereby they are fixed together short fiber and microgranule through Overheating Treatment to form a coherent unit.
Connecting elements 6 is the rectangle adhesive sticker zone of elongation, and it is in the surface of liquid-impermeable cover sheet 3, is positioned at the side away from the wearer during use.Connecting elements 6 spreads all over the overwhelming majority of liquid-impermeable cover sheet 3.The present invention is not limited by the scope of connecting elements 6, and described member can be a strip, transverse area, point, circle, or other shape and structure.The present invention is not subjected to use separately the restriction of binding agent connecting elements yet, because can use friction to connect, and the machanical fastener of other type, as snap-button, clip, belt, medicated underpants, or other known suitable analog all can use.When using the binding agent connecting elements, to utilize usually protective layer 9 make its avoid with other surface adhesion of formerly using together, otherwise can destroy this connecting elements.
When being used to absorb product, the film-shaped polymer matrix that contains the antibacterial of lactic acid producing of the present invention can be placed on the top of sepage cover sheet 2 or be placed directly in below it.Film-shaped polymer matrix can not cover all surfaces that absorbs product when preferably, the film-shaped polymer matrix that contains the antibacterial of lactic acid producing was placed.Like this, the film-shaped polymer matrix that contains the antibacterial of lactic acid producing just can not hinder and absorb the absorption of product to body fluid (as blood, urine, secretions etc.).All cover if absorb product tunicle shaped polymer matrix, dissolved by body fluid even contain the film-shaped polymer matrix of the antibacterial of lactic acid producing, before film-shaped polymer matrix was dissolved, the initial absorption that absorbs product also can be weakened.A kind of method that addresses this problem provides the absorbing articles of the film-shaped polymer matrix with antibacterial that contains lactic acid producing of the present invention, and these article have formed at least one opening through punching, can transmit body fluid by this opening.More preferably, also the carrier that is shaped as grid or loose non-woven sheet can be placed on sepage and cover on top or be placed directly in below it, described carrier is submerged in the dispersion of antibacterial of the polymer that can be used for preparing film-shaped polymer matrix of the present invention and lactic acid producing.Fig. 1 and Fig. 2 for example understand a kind of method of the film-shaped polymer matrix of placing the antibacterial that contains lactic acid producing, and wherein film-shaped polymer matrix is placed in the bar 8.
According to above-mentioned similar mode, can prepare the catamenial tampons that contains film-shaped polymer matrix.Fig. 3 and Fig. 4 (catamenial tampons shown in Fig. 3 is along the cross section of IV-IV line) have described the sketch map of the catamenial tampons 10 that comprises film-shaped polymer matrix 13 of the present invention, and wherein film-shaped polymer matrix 13 is placed on the cover sheet 11.Absorbent core 12 has also been described simultaneously.
The technical staff can utilize the above-mentioned exemplary description that contains the health product of film-shaped polymer matrix of the present invention at an easy rate, produces the health product that contains film-shaped polymer matrix of the present invention.Therefore, the present invention also comprises sanitary towel, incontinence protection, panty-liners, diaper, catamenial tampons, alternative plans such as toilet paper.
The antibacterial of lactic acid producing is embedded in also is applicable in the film-shaped polymer matrix of the present invention and increases in the pharmaceutical preparation and the survival rate of antibacterial in the food industry.
The film-shaped polymer matrix that contains the antibacterial of lactic acid producing of the present invention has several advantages.When directly joining cryodesiccated antibacterial or bacterial suspension in the health product, can not produce negative influence in order to make the intrinsic water content in the product to the survival of antibacterial, product must carry out drying.This is the process of a complexity, because entire product all needs drying, but the drying of health product also can cause the body fluid initial absorption to reduce, because some residual moistures can promote initial absorption.In addition, since will keep the survival of antibacterial, just must utilize waterproofing packing further to prevent to make moist in transportation and the storing process.Thisly all place the necessity of damp-prrof packing to cause the rising of production cost whole health product.By comparison, antibacterial is embedded in the film-shaped polymer matrix of the present invention, does not only need dry health product and use damp-prrof packing, and even after long term storage, still can keep the high viability of bacterial cell.The use of film-shaped polymer matrix of the present invention has further reduced the use of cryodesiccated bacteria preparation, and the latter is the present very general bacteria preparation form that is used for health product, but its cost height and complicated process of preparation.On the contrary, when preparation contains the film-shaped polymer matrix of antibacterial of lactic acid producing, can directly use the suspension of the antibacterial of lactic acid producing, this method cost is low and more practical.
The film-shaped polymer matrix that contains the antibacterial of lactic acid producing also can be prefabricated, is placed on afterwards on many different products, as health product etc., and do not need to carry out any special transformation when being used for different products.Equally, prepare the product that contains antibacterial maker is had special hygienic requirements.So, can reduce production costs by the film-shaped polymer matrix that contains the antibacterial of lactic acid producing in another place preparation.
Because membrane structure is very insensitive to variations in temperature in itself, therefore, the antibacterial that prevents lactic acid producing uses film-shaped polymer matrix at normal temperatures for making moist, in transportation and storage process, can obtain high stability.Equally, the drying of the bacterial cell in the exsiccant film-shaped polymer matrix causes antibacterial to have stronger thermostability.
In addition, when antibacterial mixes mutually with lyophobic dust, compare with extrusion molding or spraying commonly used, the preparation film-shaped polymer matrix is gentle more for the antibacterial of lactic acid producing.
The application of film-shaped polymer matrix that contains the antibacterial of lactic acid producing also helps antibacterial and transfers to effectiveness on the skin.When the film-shaped polymer matrix of the antibacterial that contains lactic acid producing was dissolved, the fragment of film-shaped polymer matrix can be transferred on the skin and further dissolving.Therefore, the film fragment has served as the vehicle that shifts antibacterial.Equally, the application of film-shaped polymer matrix has guaranteed that antibacterial remains on the skin of health product, has therefore guaranteed the high rate of transform.When the film-shaped polymer matrix of the antibacterial that contains lactic acid producing was used on the health product, even select surfacing commonly used for use, it is best that the rate of transform also can reach.
The specific embodiment
Therefore, the invention solves the problems relevant with the product that the antibacterial that contains lactic acid producing is provided.Below, will be by exemplary but not determinate embodiment further describes the present invention.
Embodiment 1
Contain the preparation of film-shaped polymer matrix of the antibacterial of lactic acid producing
By with poly(ethylene oxide), polyvinylpyrrolidone, polyvinyl alcohol or starch dissolution prepare the aqueous solutions of polymers that weight percent concentration is 0.1-10% in water.
1 part contains Lactobacillus plantarum 931 (preserving number (DSMZ): bacterial suspension 11918) (about 10 10CFU/ml, BioNativ AB, Box 7979, and 90719Umea Sweden) mixed 5 minutes with 9 parts of polymer solutions.Mixture is poured in the little training Ti Shi culture dish, and the amount of mixture is wanted can guarantee to obtain appropriate thickness and guarantee that number of bacteria is about 10 9The cfu/ film-shaped polymer matrix.The thickness of the film-shaped polymer matrix of the resulting antibacterial that contains lactic acid producing is preferably 50 μ m-5mm, and more preferably 100 μ m-1mm most preferably are 500 μ m-1mm.
To train the Ti Shi culture dish and put into 37 ℃, in the phjytotron of relative humidity low as far as possible (10% or following), thus water evaporates simultaneously film-shaped polymer matrix solidify, antibacterial is fixed in the film-shaped polymer matrix.
Use a w-instrument; (ADAB AnalyticalDevices AB, Stockholm Sweden) measure the water activity of the film-shaped polymer matrix of the antibacterial contain lactic acid producing to DD401102 Aqualab Serie 3TE.
The 1-2 of preparation after the film-shaped polymer matrix be within week, carries out the transferring test (stating as follows) from the absorption product that contains film-shaped polymer matrix of the present invention to skin.
Embodiment 2
The survival condition of the Lactobacillus plantarum 931 in the film-shaped polymer matrix of the present invention
It is 23 ℃ that film-shaped polymer matrix is placed temperature, and relative humidity is in 50% the phjytotron.With the film-shaped polymer matrix of embodiment 1 smeared in addition Caremelt (a kind of wax mixture, Cognis, Henkel KgaA, Dusseldorf, Germany) after, test the bacteria living situation in this film-shaped polymer matrix.The survival condition of antibacterial can be tested according to pre-set time interval in some months in the film-shaped polymer matrix.
In order to test the survival condition of the antibacterial of lactic acid producing in the film-shaped polymer matrix, this film be placed training Ti Shi culture dish, immerse among the 20ml NaCl (0.85%) and be placed on the agitating device.After 40 minutes, film-shaped polymer matrix dissolving, flat board coating culture technique by utilizing standard and MRS agar culture method (37 ℃ incubation 2 days) calculate colony-forming units (CFU) thus quantity measure the survival condition of antibacterial.The result shows that in Fig. 5 PVP wherein is a polyvinylpyrrolidone.
Embodiment 3
From having the panty-liners transfering plant lactobacillus 931 of film-shaped polymer matrix
The film-shaped polymer matrix that contains the antibacterial of lactic acid producing according to the above embodiments 1 preparation.About 1cm under the cutting 2Film-shaped polymer matrix, weigh and place on nonwoven panty-liners sample (in's that to absorb the diameter that stamps out on the product be 2.5 annulus) the top layer.
With pipette 100 μ l sodium chloride are added in this absorption product that contains germy film-shaped polymer matrix, with constant compression force (elastic cord or elastic bandage) sample are placed on volunteer's the forearm subsequently.After 2 hours, take product away, and measure the quantity of the lactobacillus that shifts on the skin.An aseptic rustless steel cylinder (diameter 2.6cm, high 2cm) is close on the skin (position that is covered by sample before), and (0.1M pH7.2) is poured in the cylinder with the 1ml phosphate buffer.With a slick Glass rod " massage " 1 minute gently on skin.Then, collect buffer with pipette and utilize dull and stereotyped coating culture technique and MRS agar method to measure CFU.
Thereby with collecting the percentage ratio that calculates the Lactobacillus plantarum of transfer from the CFU quantity of the skin part that is covered by sample divided by CFU quantity total in the film-shaped polymer matrix on the sample.The quantity that is present in the Lactobacillus plantarum of sampling point on the skin at first is seldom, particularly transfers to the quantity of the Lactobacillus plantarum 931 of skin.Therefore, behind the transferring test on skin the quantity of detected Lactobacillus plantarum all to can be considered be the result who shifts from the sample that contains Lactobacillus plantarum 931.In contrast, used the panty-liners that contains the drying bacteria preparation, described drying bacteria preparation is not embedded in the film-shaped polymer matrix.As shown in table 1, use film-shaped polymer matrix of the present invention, increased and absorbed the percentage of bacteria of transferred product to the skin.
Table 1
Polymer Concentration (w/w) MW(kDa) Transfer to the CFU quantity on the skin The initial number of CFU in the product The rate of transform (%)
PVOH 1 96 3.E+04 1.E+06 2.4
PVP 1 40 7.E+07 6.E+08 12.7
Contrast 2.E+05 4.E+07 0.4
The PVP=polyvinylpyrrolidone
The PVOH=polyvinyl alcohol

Claims (20)

1. single polymer layer base film that in pharmaceutically acceptable polymer, comprises lactic acid producing bacteria, wherein pharmaceutically acceptable polymer can prevent that bacterial cell from making moist in storage process, but it can be dissolved in body fluid.
2. single polymer layer base film according to claim 1, wherein the bacterial isolates of lactic acid producing separates skin or the urogenital from healthy human body.
3. single polymer layer base film according to claim 1, wherein the bacterial isolates of lactic acid producing is selected from Pediococcus, Lactococcus, Lactobacillus or their mixture.
4. single polymer layer base film according to claim 3, wherein the bacterial isolates of lactic acid producing is selected from Lactobacillus plantarum at least.
5. single polymer layer base film according to claim 4, wherein the bacterial isolates of lactic acid producing is selected from Lactobacillus plantarum 931 (preserving number (DSMZ): 11918) at least
6. according to each described single polymer layer base film of claim 1-5, polymer wherein is selected from polysaccharide and derivant and synthesis hydrophilic polymer and derivant thereof.
7. according to each described single polymer layer base film of claim 1-5, polymer wherein is selected from polyvinyl alcohol, poly(ethylene oxide), polyvinylpyrrolidone and starch.
8. according to each described single polymer layer base film of claim 1-5, further comprise supplementary element.
9. according to each described single polymer layer base film of claim 1-5, the thickness of this single polymer layer base film is 50 μ m-5mm.
10. according to each described single polymer layer base film of claim 1-5, the thickness of this single polymer layer base film is 100 μ m-1mm.
11. according to each described single polymer layer base film of claim 1-5, the thickness of this single polymer layer base film is 500 μ m-1mm.
12. according to each described single polymer layer base film of claim 1-5, wherein the water activity of single polymer layer base film be 0.30 or below.
13. according to each described single polymer layer base film of claim 1-5, wherein the water activity of single polymer layer base film be 0.25 or below.
14. according to each described single polymer layer base film of claim 1-5, wherein the water activity of single polymer layer base film be 0.20 or below.
15. according to each described single polymer layer base film of claim 1-5, bacterial concentration wherein is 10 7-10 14The CFU/g film.
16. according to each described single polymer layer base film of claim 1-5, bacterial concentration wherein is 10 9-10 12The CFU/g film.
17. according to each described single polymer layer base film of claim 1-5, this single polymer layer base film further comprises the lamination that is placed at least one side of single polymer layer base film.
18. a health product, as sanitary towel, panty-liners, diaper, incontinence protection, catamenial tampons, toilet paper comprises the described single polymer layer base film that contains the antibacterial of lactic acid producing according to claim 1-17.
19. preparation is according to the method for the single polymer layer base film of the described antibacterial that contains lactic acid producing of claim 1-16, its step comprises:
A) a kind of skin and the nontoxic also non-irritating aqueous solutions of polymers of mucosa of preparation to user,
B) antibacterial with lactic acid producing is dispersed in the described polymer solution,
C) in dispersion, randomly add supplementary element,
D) below 50 ℃, contain the dispersion of the antibacterial of lactic acid producing on the dry resulting inactive surfaces, thereby generate the single polymer layer base film,
E) the resulting single polymer layer base film of lamination randomly;
Wherein can random order administration step b) and c).
20. a kit utility comprises:
A) a kind of health product, as sanitary towel, panty-liners, diaper, incontinence protection, catamenial tampons, toilet paper; With
B) a kind of according to the described single polymer layer base film of claim 1-17.
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