AU2003272419B8 - Rapidly degraded reporter fusion proteins - Google Patents
Rapidly degraded reporter fusion proteins Download PDFInfo
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- AU2003272419B8 AU2003272419B8 AU2003272419A AU2003272419A AU2003272419B8 AU 2003272419 B8 AU2003272419 B8 AU 2003272419B8 AU 2003272419 A AU2003272419 A AU 2003272419A AU 2003272419 A AU2003272419 A AU 2003272419A AU 2003272419 B8 AU2003272419 B8 AU 2003272419B8
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009465 prokaryotic expression Effects 0.000 description 1
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- 230000004952 protein activity Effects 0.000 description 1
- 230000026447 protein localization Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 239000000007 protein synthesis inhibitor Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
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- 230000002285 radioactive effect Effects 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
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- 230000010474 transient expression Effects 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- 108010016264 ubiquitin-Nalpha-protein hydrolase Proteins 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
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- 101150074257 xylE gene Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41107002P | 2002-09-16 | 2002-09-16 | |
| US60/411,070 | 2002-09-16 | ||
| US41226802P | 2002-09-20 | 2002-09-20 | |
| US60/412,268 | 2002-09-20 | ||
| PCT/US2003/028939 WO2004025264A2 (en) | 2002-09-16 | 2003-09-16 | Rapidly degraded reporter fusion proteins |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| AU2003272419A1 AU2003272419A1 (en) | 2004-04-30 |
| AU2003272419B2 AU2003272419B2 (en) | 2008-08-14 |
| AU2003272419B8 true AU2003272419B8 (en) | 2008-08-21 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2003272419A Ceased AU2003272419B8 (en) | 2002-09-16 | 2003-09-16 | Rapidly degraded reporter fusion proteins |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20040146987A1 (enExample) |
| EP (2) | EP2182059A1 (enExample) |
| JP (1) | JP4528623B2 (enExample) |
| AT (1) | ATE451454T1 (enExample) |
| AU (1) | AU2003272419B8 (enExample) |
| CA (1) | CA2499221A1 (enExample) |
| DE (1) | DE60330489D1 (enExample) |
| WO (1) | WO2004025264A2 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| US7728118B2 (en) * | 2004-09-17 | 2010-06-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| WO2007058140A1 (ja) * | 2005-11-16 | 2007-05-24 | Toyo Boseki Kabushiki Kaisha | 細胞内発光イメージングのために最適化されたルシフェラーゼ遺伝子 |
| JP5083750B2 (ja) * | 2005-11-16 | 2012-11-28 | 独立行政法人産業技術総合研究所 | 細胞内発光イメージングのために最適化されたルシフェラーゼ遺伝子 |
| US20100197767A1 (en) * | 2007-07-11 | 2010-08-05 | Lior Nissim | Nucleic acid construct systems capable of daignosing or treating a cell state |
| JP5278942B2 (ja) * | 2007-12-03 | 2013-09-04 | 独立行政法人産業技術総合研究所 | タンパク質短寿命化ペプチドをコードする遺伝子及びその使用方法 |
| WO2011088421A2 (en) * | 2010-01-15 | 2011-07-21 | California Institute Of Technology | Discovery and applications of the proteolytic function of n-terminal acetylation of cellular proteins |
| US11072812B2 (en) | 2013-03-15 | 2021-07-27 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
| EP2986635B1 (en) | 2013-04-18 | 2018-10-03 | Fondazione Telethon | Effective delivery of large genes by dual aav vectors |
| EP3196303B1 (en) * | 2014-07-31 | 2020-07-22 | Transgenic Inc. | Inflammation reporter system |
| WO2016149422A1 (en) * | 2015-03-16 | 2016-09-22 | The Broad Institute, Inc. | Encoding of dna vector identity via iterative hybridization detection of a barcode transcript |
| US11174495B2 (en) | 2015-12-04 | 2021-11-16 | Board Of Regents, The University Of Texas System | Reporter system for detecting and targeting activated cells |
| CN109153997A (zh) * | 2016-05-24 | 2019-01-04 | 玫帝托克斯股份有限公司 | 编码含报告分子、底物和去稳定化部分的多肽的重组多核苷酸，含其的宿主细胞及其用途 |
| EP4269571A4 (en) | 2020-12-25 | 2025-01-01 | Kyoto University | METHOD FOR PRODUCING NAIVE HUMAN PLURIPOTENT STEM CELLS FROM SOMATIC CELLS |
| CN114921484A (zh) * | 2022-06-14 | 2022-08-19 | 四川大学华西医院 | 用于体外筛选引起基因沉默药物的报告基因组、试剂盒及其应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001064921A1 (en) * | 2000-02-29 | 2001-09-07 | The Trustees Of Columbia University In The City Of New York | Melanoma differentiation associated gene - 7 promoter and uses thereof |
| WO2002072844A1 (en) * | 2001-03-09 | 2002-09-19 | Gene Stream Pty Ltd | Novel expression vectors |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5196321A (en) * | 1986-10-02 | 1993-03-23 | Massachusetts Institute Of Technology | Methods for in vitro cleavage of ubiquitin fusion proteins |
| US6114148C1 (en) * | 1996-09-20 | 2012-05-01 | Gen Hospital Corp | High level expression of proteins |
| US5976796A (en) * | 1996-10-04 | 1999-11-02 | Loma Linda University | Construction and expression of renilla luciferase and green fluorescent protein fusion genes |
| US6306600B1 (en) * | 1998-04-17 | 2001-10-23 | Clontech Laboratories, Inc. | Rapidly degrading GFP-fusion proteins and methods of use |
| US6130313A (en) * | 1997-10-02 | 2000-10-10 | Clontech Laboratories, Inc. | Rapidly degrading GFP-fusion proteins |
| US6103313A (en) * | 1998-10-20 | 2000-08-15 | Eastman Kodak Company | Method for electrostatically assisted curtain coating at high speeds |
| GB9828709D0 (en) * | 1998-12-24 | 1999-02-17 | Novartis Ag | Assay |
-
2003
- 2003-09-16 DE DE60330489T patent/DE60330489D1/de not_active Expired - Lifetime
- 2003-09-16 AT AT03754599T patent/ATE451454T1/de not_active IP Right Cessation
- 2003-09-16 EP EP09178174A patent/EP2182059A1/en not_active Withdrawn
- 2003-09-16 EP EP03754599A patent/EP1558729B1/en not_active Expired - Lifetime
- 2003-09-16 JP JP2004536343A patent/JP4528623B2/ja not_active Expired - Fee Related
- 2003-09-16 CA CA002499221A patent/CA2499221A1/en not_active Abandoned
- 2003-09-16 WO PCT/US2003/028939 patent/WO2004025264A2/en not_active Ceased
- 2003-09-16 AU AU2003272419A patent/AU2003272419B8/en not_active Ceased
- 2003-09-16 US US10/664,341 patent/US20040146987A1/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001064921A1 (en) * | 2000-02-29 | 2001-09-07 | The Trustees Of Columbia University In The City Of New York | Melanoma differentiation associated gene - 7 promoter and uses thereof |
| WO2002072844A1 (en) * | 2001-03-09 | 2002-09-19 | Gene Stream Pty Ltd | Novel expression vectors |
Non-Patent Citations (2)
| Title |
|---|
| CORISH P et al, Protein Engineering, 1999, vol 12, p 1035-1040 * |
| LECLER G M et al, Biotechniques, 2000, vol 29, p 590-601 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004025264A3 (en) | 2005-06-09 |
| AU2003272419A1 (en) | 2004-04-30 |
| EP1558729A4 (en) | 2006-09-06 |
| JP4528623B2 (ja) | 2010-08-18 |
| EP2182059A1 (en) | 2010-05-05 |
| EP1558729B1 (en) | 2009-12-09 |
| CA2499221A1 (en) | 2004-03-25 |
| EP1558729A2 (en) | 2005-08-03 |
| JP2005538724A (ja) | 2005-12-22 |
| US20040146987A1 (en) | 2004-07-29 |
| DE60330489D1 (de) | 2010-01-21 |
| ATE451454T1 (de) | 2009-12-15 |
| AU2003272419B2 (en) | 2008-08-14 |
| WO2004025264A2 (en) | 2004-03-25 |
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Free format text: IN VOL 22, NO 32, PAGE(S) 3843 UNDER THE HEADING APPLICATIONS ACCEPTED -NAME INDEX UNDER THE NAME PROMEGA CORPORATION, APPLICATION NO. 2003272419, UND ER INID(72), CORRECT THE INVENTOR NAME TO READ ZDANOVSKAIA, MARINA |
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| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |