JP4528623B2 - 迅速分解性レポーター融合タンパク質 - Google Patents

迅速分解性レポーター融合タンパク質 Download PDF

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Publication number
JP4528623B2
JP4528623B2 JP2004536343A JP2004536343A JP4528623B2 JP 4528623 B2 JP4528623 B2 JP 4528623B2 JP 2004536343 A JP2004536343 A JP 2004536343A JP 2004536343 A JP2004536343 A JP 2004536343A JP 4528623 B2 JP4528623 B2 JP 4528623B2
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seq
nucleic acid
sequence
protein
acid molecule
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Expired - Fee Related
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JP2004536343A
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Japanese (ja)
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JP2005538724A (ja
JP2005538724A5 (enExample
Inventor
ズダノブスキー、アレクセイ
ズダノブスカヤ、マリーナ
マ、ドンピン
ウッド、キース、ヴィ．
ディー． アーモンド、ブライアン
ジー． ウッド、モニカ
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Promega Corp
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Promega Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2004536343A 2002-09-16 2003-09-16 迅速分解性レポーター融合タンパク質 Expired - Fee Related JP4528623B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41107002P 2002-09-16 2002-09-16
US41226802P 2002-09-20 2002-09-20
PCT/US2003/028939 WO2004025264A2 (en) 2002-09-16 2003-09-16 Rapidly degraded reporter fusion proteins

Publications (3)

Publication Number Publication Date
JP2005538724A JP2005538724A (ja) 2005-12-22
JP2005538724A5 JP2005538724A5 (enExample) 2007-08-09
JP4528623B2 true JP4528623B2 (ja) 2010-08-18

Family

ID=31998013

Family Applications (1)

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JP2004536343A Expired - Fee Related JP4528623B2 (ja) 2002-09-16 2003-09-16 迅速分解性レポーター融合タンパク質

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Country Link
US (1) US20040146987A1 (enExample)
EP (2) EP1558729B1 (enExample)
JP (1) JP4528623B2 (enExample)
AT (1) ATE451454T1 (enExample)
AU (1) AU2003272419B8 (enExample)
CA (1) CA2499221A1 (enExample)
DE (1) DE60330489D1 (enExample)
WO (1) WO2004025264A2 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
WO2007058140A1 (ja) * 2005-11-16 2007-05-24 Toyo Boseki Kabushiki Kaisha 細胞内発光イメージングのために最適化されたルシフェラーゼ遺伝子
JP5083750B2 (ja) * 2005-11-16 2012-11-28 独立行政法人産業技術総合研究所 細胞内発光イメージングのために最適化されたルシフェラーゼ遺伝子
ATE540115T1 (de) * 2007-07-11 2012-01-15 Yeda Res & Dev Nukleinsäurekonstruktsysteme mit fähigkeit zur diagnose oder behandlung eines zellzustands
JP5278942B2 (ja) * 2007-12-03 2013-09-04 独立行政法人産業技術総合研究所 タンパク質短寿命化ペプチドをコードする遺伝子及びその使用方法
WO2011088421A2 (en) * 2010-01-15 2011-07-21 California Institute Of Technology Discovery and applications of the proteolytic function of n-terminal acetylation of cellular proteins
WO2014151282A1 (en) 2013-03-15 2014-09-25 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
WO2014170480A1 (en) 2013-04-18 2014-10-23 Fondazione Telethon Effective delivery of large genes by dual aav vectors
EP3196303B1 (en) * 2014-07-31 2020-07-22 Transgenic Inc. Inflammation reporter system
US11046952B2 (en) * 2015-03-16 2021-06-29 The Broad Institute, Inc. Encoding of DNA vector identity via iterative hybridization detection of a barcode transcript
US11174495B2 (en) 2015-12-04 2021-11-16 Board Of Regents, The University Of Texas System Reporter system for detecting and targeting activated cells
EP3438267B1 (en) * 2016-05-24 2024-05-01 Medytox Inc. Recombinant polynucleotide coding for polypeptide comprising reporter moiety, substrate moiety and destabilizing moiety, host cell comprising same and use of same
WO2022138964A1 (ja) 2020-12-25 2022-06-30 国立大学法人京都大学 体細胞からのナイーブ型ヒトｉＰＳ細胞製造方法
CN114921484A (zh) * 2022-06-14 2022-08-19 四川大学华西医院 用于体外筛选引起基因沉默药物的报告基因组、试剂盒及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196321A (en) * 1986-10-02 1993-03-23 Massachusetts Institute Of Technology Methods for in vitro cleavage of ubiquitin fusion proteins
US6114148C1 (en) * 1996-09-20 2012-05-01 Gen Hospital Corp High level expression of proteins
US5976796A (en) * 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
US6306600B1 (en) * 1998-04-17 2001-10-23 Clontech Laboratories, Inc. Rapidly degrading GFP-fusion proteins and methods of use
US6130313A (en) * 1997-10-02 2000-10-10 Clontech Laboratories, Inc. Rapidly degrading GFP-fusion proteins
US6103313A (en) * 1998-10-20 2000-08-15 Eastman Kodak Company Method for electrostatically assisted curtain coating at high speeds
GB9828709D0 (en) * 1998-12-24 1999-02-17 Novartis Ag Assay
US6841362B1 (en) * 2000-02-29 2005-01-11 The Trustees Of Columbia University In The City Of New York Melanoma differentiation associated gene-7 promoter and uses thereof
AU2002242474B2 (en) * 2001-03-09 2004-05-20 Gene Stream Pty Ltd Novel expression vectors

Also Published As

Publication number Publication date
CA2499221A1 (en) 2004-03-25
DE60330489D1 (de) 2010-01-21
EP1558729A2 (en) 2005-08-03
EP1558729A4 (en) 2006-09-06
ATE451454T1 (de) 2009-12-15
EP1558729B1 (en) 2009-12-09
US20040146987A1 (en) 2004-07-29
EP2182059A1 (en) 2010-05-05
WO2004025264A3 (en) 2005-06-09
JP2005538724A (ja) 2005-12-22
WO2004025264A2 (en) 2004-03-25
AU2003272419B2 (en) 2008-08-14
AU2003272419B8 (en) 2008-08-21
AU2003272419A1 (en) 2004-04-30

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