AU2002362377B2 - Lactobacillus N-deoxyribosyl transferases, corresponding nucleotide sequences and their uses - Google Patents

Info

Publication number

AU2002362377B2

AU2002362377B2 AU2002362377A AU2002362377A AU2002362377B2 AU 2002362377 B2 AU2002362377 B2 AU 2002362377B2 AU 2002362377 A AU2002362377 A AU 2002362377A AU 2002362377 A AU2002362377 A AU 2002362377A AU 2002362377 B2 AU2002362377 B2 AU 2002362377B2

Authority

AU

Australia

Prior art keywords

leu

asp

lys

tyr

gly

Prior art date

2001-09-14

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Ceased

Links

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• 108020004207 Nucleoside deoxyribosyltransferase Proteins 0.000 title claims abstract description 32
• 241000186660 Lactobacillus Species 0.000 title claims description 18
• 229940039696 lactobacillus Drugs 0.000 title claims description 14
• 108091028043 Nucleic acid sequence Proteins 0.000 title description 16
• 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 113
• 229920001184 polypeptide Polymers 0.000 claims abstract description 110
• 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 110
• 238000000034 method Methods 0.000 claims abstract description 75
• 239000012634 fragment Substances 0.000 claims abstract description 48
• 150000007523 nucleic acids Chemical class 0.000 claims abstract description 40
• 230000000694 effects Effects 0.000 claims abstract description 31
• 239000013598 vector Substances 0.000 claims abstract description 19
• 238000003786 synthesis reaction Methods 0.000 claims abstract description 15
• 230000015572 biosynthetic process Effects 0.000 claims abstract description 11
• 238000010367 cloning Methods 0.000 claims abstract description 11
• 241001465754 Metazoa Species 0.000 claims abstract description 9
• 239000013604 expression vector Substances 0.000 claims abstract description 7
• 239000005547 deoxyribonucleotide Substances 0.000 claims abstract description 6
• 125000002637 deoxyribonucleotide group Chemical group 0.000 claims abstract description 6
• 239000013599 cloning vector Substances 0.000 claims abstract 3
• 101150048702 ntd gene Proteins 0.000 claims description 45
• UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims description 44
• 108091033319 polynucleotide Proteins 0.000 claims description 41
• 102000040430 polynucleotide Human genes 0.000 claims description 41
• 239000002157 polynucleotide Substances 0.000 claims description 41
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 39
• 150000001413 amino acids Chemical group 0.000 claims description 37
• 239000000523 sample Substances 0.000 claims description 37
• 230000008569 process Effects 0.000 claims description 29
• 238000006243 chemical reaction Methods 0.000 claims description 26
• 230000003321 amplification Effects 0.000 claims description 22
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 22
• 241000894006 Bacteria Species 0.000 claims description 21
• 240000002605 Lactobacillus helveticus Species 0.000 claims description 19
• 230000001580 bacterial effect Effects 0.000 claims description 15
• 239000005549 deoxyribonucleoside Substances 0.000 claims description 15
• 238000001514 detection method Methods 0.000 claims description 14
• LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 claims description 14
• 101000983111 Homo sapiens Serine/threonine-protein kinase PAK 6 Proteins 0.000 claims description 12
• 235000013967 Lactobacillus helveticus Nutrition 0.000 claims description 12
• 102100026840 Serine/threonine-protein kinase PAK 6 Human genes 0.000 claims description 12
• 230000002255 enzymatic effect Effects 0.000 claims description 12
• 238000006467 substitution reaction Methods 0.000 claims description 12
• RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 12
• 229940054346 lactobacillus helveticus Drugs 0.000 claims description 11
• 238000001727 in vivo Methods 0.000 claims description 9
• 241001134654 Lactobacillus leichmannii Species 0.000 claims description 7
• 238000004519 manufacturing process Methods 0.000 claims description 7
• ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Natural products O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 6
• 229940113082 thymine Drugs 0.000 claims description 6
• 229930024421 Adenine Natural products 0.000 claims description 5
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 5
• 241000282414 Homo sapiens Species 0.000 claims description 5
• 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
• 229960000643 adenine Drugs 0.000 claims description 5
• 238000000338 in vitro Methods 0.000 claims description 5
• ZBNZAJFNDPPMDT-UHFFFAOYSA-N 1h-imidazole-5-carboxamide Chemical compound NC(=O)C1=CNC=N1 ZBNZAJFNDPPMDT-UHFFFAOYSA-N 0.000 claims description 4
• 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 4
• BVIAOQMSVZHOJM-UHFFFAOYSA-N N(6),N(6)-dimethyladenine Chemical compound CN(C)C1=NC=NC2=C1N=CN2 BVIAOQMSVZHOJM-UHFFFAOYSA-N 0.000 claims description 4
• FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 claims description 4
• 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 4
• 239000006166 lysate Substances 0.000 claims description 4
• 150000003212 purines Chemical class 0.000 claims description 4
• 241000196324 Embryophyta Species 0.000 claims description 3
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• 150000003230 pyrimidines Chemical class 0.000 claims description 3
• 230000028327 secretion Effects 0.000 claims description 3
• ZEWJFUNFEABPGL-UHFFFAOYSA-N 1,2,4-triazole-3-carboxamide Chemical compound NC(=O)C=1N=CNN=1 ZEWJFUNFEABPGL-UHFFFAOYSA-N 0.000 claims description 2
• RMFWVOLULURGJI-UHFFFAOYSA-N 2,6-dichloro-7h-purine Chemical compound ClC1=NC(Cl)=C2NC=NC2=N1 RMFWVOLULURGJI-UHFFFAOYSA-N 0.000 claims description 2
• NMUSYJAQQFHJEW-KVTDHHQDSA-N 5-azacytidine Chemical compound O=C1N=C(N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 NMUSYJAQQFHJEW-KVTDHHQDSA-N 0.000 claims description 2
• ZKBQDFAWXLTYKS-UHFFFAOYSA-N 6-Chloro-1H-purine Chemical compound ClC1=NC=NC2=C1NC=N2 ZKBQDFAWXLTYKS-UHFFFAOYSA-N 0.000 claims description 2
• XZWMZFQOHTWGQE-UHFFFAOYSA-N 6-azathymine Chemical compound CC1=NNC(=O)NC1=O XZWMZFQOHTWGQE-UHFFFAOYSA-N 0.000 claims description 2
• RYYIULNRIVUMTQ-UHFFFAOYSA-N 6-chloroguanine Chemical compound NC1=NC(Cl)=C2N=CNC2=N1 RYYIULNRIVUMTQ-UHFFFAOYSA-N 0.000 claims description 2
• SYMHUEFSSMBHJA-UHFFFAOYSA-N 6-methylpurine Chemical compound CC1=NC=NC2=C1NC=N2 SYMHUEFSSMBHJA-UHFFFAOYSA-N 0.000 claims description 2
• YEGKYFQLKYGHAR-UHFFFAOYSA-N 6-methylthioguanine Chemical compound CSC1=NC(N)=NC2=C1NC=N2 YEGKYFQLKYGHAR-UHFFFAOYSA-N 0.000 claims description 2
• UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 claims description 2
• 229960002756 azacitidine Drugs 0.000 claims description 2
• 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 2
• 150000002460 imidazoles Chemical class 0.000 claims description 2
• 238000006116 polymerization reaction Methods 0.000 claims description 2
• YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 claims 7
• AWZKCUCQJNTBAD-SRVKXCTJSA-N Ala-Leu-Lys Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCCCN AWZKCUCQJNTBAD-SRVKXCTJSA-N 0.000 claims 6
• JZWZACGUZVCQPS-RNJOBUHISA-N Val-Ile-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](C(C)C)N JZWZACGUZVCQPS-RNJOBUHISA-N 0.000 claims 6
• 108010050848 glycylleucine Proteins 0.000 claims 6
• CREYEAPXISDKSB-FQPOAREZSA-N Ala-Thr-Tyr Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O CREYEAPXISDKSB-FQPOAREZSA-N 0.000 claims 5
• 241000282326 Felis catus Species 0.000 claims 5
• POZULHZYLPGXMR-ONGXEEELSA-N Leu-Gly-Val Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@@H](C(C)C)C(O)=O POZULHZYLPGXMR-ONGXEEELSA-N 0.000 claims 5
• CXGLFEOYCJFKPR-RCWTZXSCSA-N Pro-Thr-Val Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(O)=O CXGLFEOYCJFKPR-RCWTZXSCSA-N 0.000 claims 5
• 108010068265 aspartyltyrosine Proteins 0.000 claims 5
• 108010054813 diprotin B Proteins 0.000 claims 5
• 108010085325 histidylproline Proteins 0.000 claims 5
• 108010034529 leucyl-lysine Proteins 0.000 claims 5
• 108010054155 lysyllysine Proteins 0.000 claims 5
• XUCHENWTTBFODJ-FXQIFTODSA-N Ala-Met-Ala Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(O)=O XUCHENWTTBFODJ-FXQIFTODSA-N 0.000 claims 4
• RCFGLXMZDYNRSC-CIUDSAMLSA-N Asn-Lys-Ala Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O RCFGLXMZDYNRSC-CIUDSAMLSA-N 0.000 claims 4
• MYTHOBCLNIOFBL-SRVKXCTJSA-N Asn-Ser-Tyr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O MYTHOBCLNIOFBL-SRVKXCTJSA-N 0.000 claims 4
• XAJRHVUUVUPFQL-ACZMJKKPSA-N Asp-Glu-Asp Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O XAJRHVUUVUPFQL-ACZMJKKPSA-N 0.000 claims 4
• DWOGMPWRQQWPPF-GUBZILKMSA-N Asp-Leu-Glu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O DWOGMPWRQQWPPF-GUBZILKMSA-N 0.000 claims 4
• UGVQELHRNUDMAA-BYPYZUCNSA-N Gly-Ala-Gly Chemical compound [NH3+]CC(=O)N[C@@H](C)C(=O)NCC([O-])=O UGVQELHRNUDMAA-BYPYZUCNSA-N 0.000 claims 4
• CVFOYJJOZYYEPE-KBPBESRZSA-N Gly-Lys-Tyr Chemical compound [H]NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O CVFOYJJOZYYEPE-KBPBESRZSA-N 0.000 claims 4
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• RDFIVFHPOSOXMW-ACRUOGEOSA-N Leu-Tyr-Phe Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O RDFIVFHPOSOXMW-ACRUOGEOSA-N 0.000 claims 4
• PLDJDCJLRCYPJB-VOAKCMCISA-N Lys-Lys-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PLDJDCJLRCYPJB-VOAKCMCISA-N 0.000 claims 4
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• AJHCSUXXECOXOY-UHFFFAOYSA-N N-glycyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)CN)C(O)=O)=CNC2=C1 AJHCSUXXECOXOY-UHFFFAOYSA-N 0.000 claims 4
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