AU2002240033B2 - Anti-bacterial vaccine compositions - Google Patents
Anti-bacterial vaccine compositions Download PDFInfo
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- AU2002240033B2 AU2002240033B2 AU2002240033A AU2002240033A AU2002240033B2 AU 2002240033 B2 AU2002240033 B2 AU 2002240033B2 AU 2002240033 A AU2002240033 A AU 2002240033A AU 2002240033 A AU2002240033 A AU 2002240033A AU 2002240033 B2 AU2002240033 B2 AU 2002240033B2
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- virulence
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- multocida
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- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
- A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
Landscapes
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- Organic Chemistry (AREA)
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- Engineering & Computer Science (AREA)
- Oncology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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| US09/809,665 US6790950B2 (en) | 1999-04-09 | 2001-03-15 | Anti-bacterial vaccine compositions |
| US09/809,665 | 2001-03-15 | ||
| PCT/US2002/001971 WO2002075507A2 (en) | 2001-03-15 | 2002-01-17 | Anti-bacterial vaccine compositions |
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| AU2002240033A1 AU2002240033A1 (en) | 2003-03-27 |
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| SI (1) | SI1368456T1 (enExample) |
| TW (4) | TWI335935B (enExample) |
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| US6455323B1 (en) * | 1997-07-03 | 2002-09-24 | Pharmacia & Upjohn Company | Anti-bacterial methods and materials |
| US6790950B2 (en) * | 1999-04-09 | 2004-09-14 | Pharmacia & Upjohn Company | Anti-bacterial vaccine compositions |
| JP4892134B2 (ja) * | 1999-04-09 | 2012-03-07 | ファルマシア・アンド・アップジョン・カンパニー・エルエルシー | 抗菌ワクチン組成物 |
| WO2002034771A2 (en) | 2000-10-27 | 2002-05-02 | Chiron Srl | Nucleic acids and proteins from streptococcus groups a & b |
| US7449178B2 (en) * | 2002-04-05 | 2008-11-11 | Merial Limited | Attenuated gram negative bacteria |
| US7794734B2 (en) * | 2002-10-30 | 2010-09-14 | The Board Of Regents For Oklahoma State University | Mannheimia haemolytica chimeric outer membrane protein PlpE and leukotoxin epitopes as a vaccine or vaccine component against shipping fever |
| GB0228691D0 (en) * | 2002-12-09 | 2003-01-15 | Imp College Innovations Ltd | Bacterial virulence genes |
| EP1648500B1 (en) | 2003-07-31 | 2014-07-09 | Novartis Vaccines and Diagnostics, Inc. | Immunogenic compositions for streptococcus pyogenes |
| US8945589B2 (en) | 2003-09-15 | 2015-02-03 | Novartis Vaccines And Diagnostics, Srl | Immunogenic compositions for Streptococcus agalactiae |
| US9107864B2 (en) * | 2004-06-07 | 2015-08-18 | Qu Biologics Inc. | Tissue targeted antigenic activation of the immune response to treat cancers |
| EP2073829B1 (en) * | 2006-10-05 | 2012-06-20 | New York Blood Center, Inc. | Stabilized therapeutic small helical antiviral peptides |
| US8937154B2 (en) | 2006-10-05 | 2015-01-20 | New York Blood Center, Inc. | Stabilized therapeutic small helical antiviral peptides |
| AU2008299376B2 (en) | 2007-09-12 | 2013-02-28 | Glaxosmithkline Biologicals S.A. | GAS57 mutant antigens and GAS57 antibodies |
| JP2011522796A (ja) | 2008-05-06 | 2011-08-04 | ニューヨーク ブラッド センター， インコーポレイテッド | 抗ウイルス細胞透過性ペプチド |
| CA2729818A1 (en) * | 2008-07-03 | 2010-01-07 | Biotechnology Research And Development Corporation | Vaccines comprising pasteurella multocida recombinant filamentous hemagglutinin peptides |
| CA2774636C (en) | 2009-09-25 | 2019-05-21 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Neutralizing antibodies to hiv-1 and their use |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9757445B2 (en) | 2013-11-01 | 2017-09-12 | Merial Inc. | Attenuated Pasteurella multocida vaccines and methods of making and use thereof |
| CN116593695B (zh) * | 2022-12-29 | 2025-08-05 | 重庆澳龙生物制品有限公司 | 一种牛多杀性巴氏杆菌荚膜a型elisa抗体检测试剂盒及其制备方法和应用 |
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| US4735801A (en) | 1982-09-07 | 1988-04-05 | Board Of Trustees Of Leland Stanford Jr. University | Novel non-reverting salmonella live vaccines |
| US5077044A (en) | 1980-05-19 | 1991-12-31 | The Board Of Trustees Of The Leland Stanford Jr. University | Novel non-reverting shigella live vaccines |
| SE8205892D0 (sv) | 1982-10-18 | 1982-10-18 | Bror Morein | Immunogent membranproteinkomplex, sett for framstellning och anvendning derav som immunstimulerande medel och sasom vaccin |
| SE8405493D0 (sv) | 1984-11-01 | 1984-11-01 | Bror Morein | Immunogent komplex samt sett for framstellning derav och anvendning derav som immunstimulerande medel |
| US4877612A (en) | 1985-05-20 | 1989-10-31 | Frank M. Berger | Immunological adjuvant and process for preparing the same, pharmaceutical compositions, and process |
| DK166762B1 (da) | 1986-01-14 | 1993-07-12 | Nederlanden Staat | Fremgangsmaade til fremstilling af immunogenkomplekser og farmaceutisk sammensaetning indeholdende saadanne komplekser |
| US4806352A (en) | 1986-04-15 | 1989-02-21 | Ribi Immunochem Research Inc. | Immunological lipid emulsion adjuvant |
| CA1340522C (en) | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
| IL86583A0 (en) | 1987-06-04 | 1988-11-15 | Molecular Eng Ass | Vaccine containing a derivative of a microbe and method for the production thereof |
| PL170938B1 (pl) | 1991-03-05 | 1997-02-28 | Wellcome Found | Sposób wytwarzania szczepionki przeciwko infekcjom Salmonella PL PL |
| US5840312A (en) | 1991-05-02 | 1998-11-24 | Institut Pasteur | Recombinant Bacillus anthracis strains unable to produce the lethal factor protein or edema factor protein |
| WO1994011024A1 (en) | 1992-11-06 | 1994-05-26 | Regents Of The University Of Minnesota | Composition protective against p. multocida pasteurellosis infection |
| US5585277A (en) | 1993-06-21 | 1996-12-17 | Scriptgen Pharmaceuticals, Inc. | Screening method for identifying ligands for target proteins |
| WO1995020652A1 (de) | 1994-01-28 | 1995-08-03 | Medigene Gmbh | Verfahren zur bestimmung der aktivität eines regulatorischen faktors sowie verwendung dieses verfahrens |
| KR100445103B1 (ko) | 1994-12-09 | 2004-12-04 | 임페리얼 컬리지 이노베이션스 리미티드 | 유전자의동정 |
| GB9518220D0 (en) | 1995-09-06 | 1995-11-08 | Medical Res Council | Checkpoint gene |
| BR9713195A (pt) * | 1996-09-06 | 1999-12-21 | Peter Ruhdal Jensen | Método de aperfeiçoamento da produção de biomassa ou de um produto desejado de uma célula. |
| US6673538B1 (en) * | 1997-07-25 | 2004-01-06 | The Trustees Of Boston University | Methods and compositions for designing vaccines |
| US6790950B2 (en) * | 1999-04-09 | 2004-09-14 | Pharmacia & Upjohn Company | Anti-bacterial vaccine compositions |
| JP4892134B2 (ja) | 1999-04-09 | 2012-03-07 | ファルマシア・アンド・アップジョン・カンパニー・エルエルシー | 抗菌ワクチン組成物 |
| US6376211B1 (en) * | 1999-07-12 | 2002-04-23 | Xoma Technology Ltd. | Agents and methods for inhibiting F1/F0 ATPase |
| DE60124526D1 (de) * | 2000-04-27 | 2006-12-28 | Max Planck Gesellschaft | Methode zur identifizierung von helicobacter antigenen |
-
2001
- 2001-03-15 US US09/809,665 patent/US6790950B2/en not_active Expired - Lifetime
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- 2002-01-17 MX MXPA03008296A patent/MXPA03008296A/es active IP Right Grant
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- 2002-01-17 CA CA2852293A patent/CA2852293A1/en not_active Abandoned
- 2002-01-17 ES ES02705915T patent/ES2362041T3/es not_active Expired - Lifetime
- 2002-01-17 EP EP02705915A patent/EP1368456B1/en not_active Expired - Lifetime
- 2002-01-17 AU AU2002240033A patent/AU2002240033B2/en not_active Expired
- 2002-01-17 NZ NZ528208A patent/NZ528208A/en not_active IP Right Cessation
- 2002-01-17 DE DE60239318T patent/DE60239318D1/de not_active Expired - Lifetime
- 2002-01-17 DK DK02705915.3T patent/DK1368456T3/da active
- 2002-01-17 WO PCT/US2002/001971 patent/WO2002075507A2/en not_active Ceased
- 2002-01-17 JP JP2002574050A patent/JP2005502315A/ja active Pending
- 2002-01-17 AT AT02705915T patent/ATE500316T1/de active
- 2002-01-17 SI SI200230939T patent/SI1368456T1/sl unknown
- 2002-01-17 PT PT02705915T patent/PT1368456E/pt unknown
- 2002-02-07 AR ARP020100402A patent/AR035745A1/es not_active Application Discontinuation
- 2002-02-08 TW TW099122913A patent/TWI335935B/zh not_active IP Right Cessation
- 2002-02-08 TW TW099122912A patent/TWI335934B/zh not_active IP Right Cessation
- 2002-02-08 TW TW091102640A patent/TWI328035B/zh not_active IP Right Cessation
- 2002-02-08 TW TW097148939A patent/TWI334884B/zh not_active IP Right Cessation
- 2002-02-27 PE PE2002000160A patent/PE20021008A1/es not_active Application Discontinuation
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| WO2002075507A2 (en) | 2002-09-26 |
| TW201040261A (en) | 2010-11-16 |
| ATE500316T1 (de) | 2011-03-15 |
| TWI334884B (en) | 2010-12-21 |
| NZ528208A (en) | 2008-04-30 |
| DE60239318D1 (de) | 2011-04-14 |
| CA2438315C (en) | 2014-09-02 |
| AR035745A1 (es) | 2004-07-07 |
| EP1368456B1 (en) | 2011-03-02 |
| MXPA03008296A (es) | 2007-05-28 |
| TWI328035B (en) | 2010-08-01 |
| JP2005502315A (ja) | 2005-01-27 |
| US20050003512A1 (en) | 2005-01-06 |
| PT1368456E (pt) | 2011-06-01 |
| SI1368456T1 (sl) | 2011-06-30 |
| CY1112014T1 (el) | 2015-11-04 |
| JP2011097938A (ja) | 2011-05-19 |
| US20040110268A1 (en) | 2004-06-10 |
| US6790950B2 (en) | 2004-09-14 |
| CA2438315A1 (en) | 2002-09-26 |
| ES2362041T3 (es) | 2011-06-27 |
| PE20021008A1 (es) | 2002-11-13 |
| DK1368456T3 (da) | 2011-05-23 |
| WO2002075507B1 (en) | 2003-12-11 |
| AR071888A2 (es) | 2010-07-21 |
| TW200916577A (en) | 2009-04-16 |
| CA2852293A1 (en) | 2002-09-26 |
| TWI335935B (en) | 2011-01-11 |
| TW201040262A (en) | 2010-11-16 |
| WO2002075507A3 (en) | 2003-09-12 |
| TWI335934B (en) | 2011-01-11 |
| US7476391B2 (en) | 2009-01-13 |
| EP1368456A2 (en) | 2003-12-10 |
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