AU2001284295A1 - Method of sequencing dna - Google Patents

Method of sequencing dna

Info

Publication number
AU2001284295A1
AU2001284295A1 AU2001284295A AU8429501A AU2001284295A1 AU 2001284295 A1 AU2001284295 A1 AU 2001284295A1 AU 2001284295 A AU2001284295 A AU 2001284295A AU 8429501 A AU8429501 A AU 8429501A AU 2001284295 A1 AU2001284295 A1 AU 2001284295A1
Authority
AU
Australia
Prior art keywords
nucleotide
base
analogue
target position
incorporated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001284295A
Other languages
English (en)
Inventor
Mostafa Ronaghi
Annika Tallsjo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pyrosequencing AB
Original Assignee
Pyrosequencing AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pyrosequencing AB filed Critical Pyrosequencing AB
Publication of AU2001284295A1 publication Critical patent/AU2001284295A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2001284295A 2000-09-07 2001-09-07 Method of sequencing dna Abandoned AU2001284295A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0021977.4 2000-09-07
GBGB0021977.4A GB0021977D0 (en) 2000-09-07 2000-09-07 Method of sequencing DNA
PCT/GB2001/004015 WO2002020836A2 (en) 2000-09-07 2001-09-07 Method of sequencing dna

Publications (1)

Publication Number Publication Date
AU2001284295A1 true AU2001284295A1 (en) 2002-03-22

Family

ID=9899042

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001284295A Abandoned AU2001284295A1 (en) 2000-09-07 2001-09-07 Method of sequencing dna

Country Status (10)

Country Link
US (2) US7459311B2 (es)
EP (1) EP1317568B1 (es)
JP (1) JP4271439B2 (es)
AT (1) ATE395435T1 (es)
AU (1) AU2001284295A1 (es)
CA (1) CA2421492C (es)
DE (1) DE60134030D1 (es)
ES (1) ES2305099T3 (es)
GB (1) GB0021977D0 (es)
WO (1) WO2002020836A2 (es)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7141370B2 (en) 2001-07-03 2006-11-28 The Board Of Trustees Of The Leland Stanford Junior University Bioluminescence regenerative cycle (BRC) for nucleic acid quantification
AU2003265185A1 (en) * 2002-09-27 2004-04-19 Biotage Ab New sequencing method for sequencing rna molecules
GB0300802D0 (en) * 2003-01-14 2003-02-12 Murray James A H Method for detecting DNA polymerisation
EP2159285B1 (en) 2003-01-29 2012-09-26 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20070111213A1 (en) 2003-10-28 2007-05-17 Lu Wang Primers, methods and kits for amplifying or detecting human leukocyte antigen alleles
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
US20060281100A1 (en) * 2005-06-14 2006-12-14 Shen Gene G Thiotriphosphate nucleotide dye terminators
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US8637317B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
US8613889B2 (en) 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7851184B2 (en) * 2006-04-18 2010-12-14 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification method and apparatus
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
US20110311980A1 (en) * 2008-12-15 2011-12-22 Advanced Liquid Logic, Inc. Nucleic Acid Amplification and Sequencing on a Droplet Actuator
US8853373B2 (en) 2009-08-14 2014-10-07 Hitachi, Ltd. Method and reagent for gene sequence analysis
US8916347B2 (en) 2010-02-19 2014-12-23 Agency For Science, Technology And Research Integrated microfluidic and solid state pyrosequencing systems
EP2580353B1 (en) 2010-06-11 2015-07-29 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
US8666678B2 (en) 2010-10-27 2014-03-04 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
WO2012103031A2 (en) 2011-01-25 2012-08-02 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
WO2012138921A1 (en) 2011-04-08 2012-10-11 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
KR102003660B1 (ko) 2011-07-13 2019-07-24 더 멀티플 마이얼로머 리서치 파운데이션, 인크. 데이터 수집 및 분배 방법
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
CA2874413A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US9884893B2 (en) 2012-05-21 2018-02-06 Distributed Bio, Inc. Epitope focusing by variable effective antigen surface concentration
CN104583421A (zh) 2012-07-19 2015-04-29 阿瑞奥萨诊断公司 遗传变体的基于多重的顺序连接的检测
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
WO2014200579A1 (en) 2013-06-13 2014-12-18 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
EP3191604B1 (en) 2014-09-09 2021-04-14 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
CN104391029B (zh) * 2014-11-17 2016-09-07 中国科学院化学研究所 一种用于测定碱性磷酸酶活性的电化学方法
JP7019200B2 (ja) 2017-11-13 2022-02-15 ザ マルチプル ミエローマ リサーチ ファウンデーション, インコーポレイテッド 統合された、分子、オーミクス、免疫療法、代謝、エピジェネティック、および臨床のデータベース
CN109797195A (zh) * 2019-01-18 2019-05-24 东南大学 基于分析pcr副产物焦磷酸的特异pcr产物快速检测方法及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO155316C (no) 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
GB9210168D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Method of sequencing dna
GB9311241D0 (en) 1993-06-01 1993-07-21 Celsis Ltd Reagents for use in bioluminescence
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) * 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2000055917A (ja) 1998-08-05 2000-02-25 Mitsubishi Kagaku Bio-Clinical Laboratories Inc 抗抗原−抗体複合体抗体を用いた検出または測定方法
EP1757696A3 (en) 1998-12-22 2008-10-29 Microscience Limited Group B streptococcus proteins, and their use
GB9901475D0 (en) * 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing

Also Published As

Publication number Publication date
US7648824B2 (en) 2010-01-19
US20090155801A1 (en) 2009-06-18
ES2305099T3 (es) 2008-11-01
GB0021977D0 (en) 2000-10-25
JP4271439B2 (ja) 2009-06-03
CA2421492C (en) 2010-05-04
CA2421492A1 (en) 2002-03-14
WO2002020836A2 (en) 2002-03-14
US7459311B2 (en) 2008-12-02
JP2004508054A (ja) 2004-03-18
US20040142330A1 (en) 2004-07-22
WO2002020836A3 (en) 2003-02-06
EP1317568A2 (en) 2003-06-11
EP1317568B1 (en) 2008-05-14
DE60134030D1 (de) 2008-06-26
ATE395435T1 (de) 2008-05-15

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