WO2003019189A1 - Microreseau de genes a phase homogene - Google Patents

Microreseau de genes a phase homogene Download PDF

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Publication number
WO2003019189A1
WO2003019189A1 PCT/CN2002/000591 CN0200591W WO03019189A1 WO 2003019189 A1 WO2003019189 A1 WO 2003019189A1 CN 0200591 W CN0200591 W CN 0200591W WO 03019189 A1 WO03019189 A1 WO 03019189A1
Authority
WO
WIPO (PCT)
Prior art keywords
templet
gene microarray
sequences
homogeneous phase
nucleic acid
Prior art date
Application number
PCT/CN2002/000591
Other languages
English (en)
Chinese (zh)
Inventor
Wei Cao
Wenquan Li
Yuejian Zhang
Jun Song
Original Assignee
Wei Cao
Wenquan Li
Yuejian Zhang
Jun Song
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wei Cao, Wenquan Li, Yuejian Zhang, Jun Song filed Critical Wei Cao
Publication of WO2003019189A1 publication Critical patent/WO2003019189A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un microréseau de gènes à phase homogène comportant un plateau garni de 4-100.000 alvéoles à réaction. Un groupe d'amorces à templet de séquences artificielles tient dans l'une au moins des alvéoles à réaction. Ce groupe d'amorces inclut des paires d'amorces oligonucléotides pouvant être spécialement reliées aux séquences de vérification des besoins et capables d'activer le processus d'amplification de l'acide nucléique cible, l'une au moins de ces amorces étant une amorce à templet de séquences artificielles. Cette amorce à templet de séquences artificielles comprend (a) des zones de combinaison spéciale, et (b) des zones générales. L'invention concerne également un procédé de détection et/ou d'analyse quantitative des séquences d'acides nucléiques au moyen de ce microréseau de gènes. L'invention convient particulièrement pour la détection et/ou l'analyse quantitative des mutations et expression du génome ainsi que des pathogènes des échantillons, et cela, facilement, avec sensibilité et exactitude.
PCT/CN2002/000591 2001-08-29 2002-08-26 Microreseau de genes a phase homogene WO2003019189A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CNB011265787A CN1186457C (zh) 2001-08-29 2001-08-29 均相基因矩阵
CN01126578.7 2001-08-29

Publications (1)

Publication Number Publication Date
WO2003019189A1 true WO2003019189A1 (fr) 2003-03-06

Family

ID=4666590

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2002/000591 WO2003019189A1 (fr) 2001-08-29 2002-08-26 Microreseau de genes a phase homogene

Country Status (2)

Country Link
CN (1) CN1186457C (fr)
WO (1) WO2003019189A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005087950A1 (fr) * 2004-03-12 2005-09-22 Canon Kabushiki Kaisha Procédé de détection d'acide nucléique utilisant l'amplification sur microplaquette
US8765576B2 (en) 2007-02-28 2014-07-01 Shin-Etsu Chemical Co., Ltd. Process for producing laminated substrate and laminated substrate
US8871737B2 (en) 2010-09-22 2014-10-28 Alios Biopharma, Inc. Substituted nucleotide analogs
US8916538B2 (en) 2012-03-21 2014-12-23 Vertex Pharmaceuticals Incorporated Solid forms of a thiophosphoramidate nucleotide prodrug
US8980865B2 (en) 2011-12-22 2015-03-17 Alios Biopharma, Inc. Substituted nucleotide analogs
US9012427B2 (en) 2012-03-22 2015-04-21 Alios Biopharma, Inc. Pharmaceutical combinations comprising a thionucleotide analog

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1248702A (zh) * 1999-09-03 2000-03-29 何农跃 Pcr微阵列探针循环检测型生物芯片
WO2001048242A2 (fr) * 1999-12-29 2001-07-05 Mergen Ltd. Procedes d'amplification et de detection de plusieurs polynucleotides sur un support en phase solide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1248702A (zh) * 1999-09-03 2000-03-29 何农跃 Pcr微阵列探针循环检测型生物芯片
WO2001048242A2 (fr) * 1999-12-29 2001-07-05 Mergen Ltd. Procedes d'amplification et de detection de plusieurs polynucleotides sur un support en phase solide

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005087950A1 (fr) * 2004-03-12 2005-09-22 Canon Kabushiki Kaisha Procédé de détection d'acide nucléique utilisant l'amplification sur microplaquette
US8765576B2 (en) 2007-02-28 2014-07-01 Shin-Etsu Chemical Co., Ltd. Process for producing laminated substrate and laminated substrate
US8871737B2 (en) 2010-09-22 2014-10-28 Alios Biopharma, Inc. Substituted nucleotide analogs
US9278990B2 (en) 2010-09-22 2016-03-08 Alios Biopharma, Inc. Substituted nucleotide analogs
US8980865B2 (en) 2011-12-22 2015-03-17 Alios Biopharma, Inc. Substituted nucleotide analogs
US9605018B2 (en) 2011-12-22 2017-03-28 Alios Biopharma, Inc. Substituted nucleotide analogs
US8916538B2 (en) 2012-03-21 2014-12-23 Vertex Pharmaceuticals Incorporated Solid forms of a thiophosphoramidate nucleotide prodrug
US9394330B2 (en) 2012-03-21 2016-07-19 Alios Biopharma, Inc. Solid forms of a thiophosphoramidate nucleotide prodrug
US9856284B2 (en) 2012-03-21 2018-01-02 Alios Biopharma, Inc. Solid forms of a thiophosphoramidate nucleotide prodrug
US9012427B2 (en) 2012-03-22 2015-04-21 Alios Biopharma, Inc. Pharmaceutical combinations comprising a thionucleotide analog

Also Published As

Publication number Publication date
CN1186457C (zh) 2005-01-26
CN1407113A (zh) 2003-04-02

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