WO1990009457A3 - Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation - Google Patents

Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation Download PDF

Info

Publication number
WO1990009457A3
WO1990009457A3 PCT/US1990/000866 US9000866W WO9009457A3 WO 1990009457 A3 WO1990009457 A3 WO 1990009457A3 US 9000866 W US9000866 W US 9000866W WO 9009457 A3 WO9009457 A3 WO 9009457A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
primer pairs
oligonucleotide primer
employ
gene amplification
Prior art date
Application number
PCT/US1990/000866
Other languages
English (en)
Other versions
WO1990009457A2 (fr
Inventor
Mark P Pasek
Original Assignee
Biogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Inc filed Critical Biogen Inc
Publication of WO1990009457A2 publication Critical patent/WO1990009457A2/fr
Publication of WO1990009457A3 publication Critical patent/WO1990009457A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne des procédés permettant d'amplifier des séquences isolées d'acide nucléique, notamment des séquences d'ADN, dont ces séquences en nucléotide ne sont pas connues. On décrit également des paires d'amorce d'oligonucléotides à utiliser dans les procédés d'amplification de la présente invention.
PCT/US1990/000866 1989-02-14 1990-02-14 Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation WO1990009457A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31076389A 1989-02-14 1989-02-14
US310,763 1989-02-14

Publications (2)

Publication Number Publication Date
WO1990009457A2 WO1990009457A2 (fr) 1990-08-23
WO1990009457A3 true WO1990009457A3 (fr) 1990-10-04

Family

ID=23203992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/000866 WO1990009457A2 (fr) 1989-02-14 1990-02-14 Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation

Country Status (4)

Country Link
EP (1) EP0458909A1 (fr)
AU (1) AU5404890A (fr)
CA (1) CA2046919A1 (fr)
WO (1) WO1990009457A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0379559B1 (fr) * 1988-06-24 1996-10-23 Amgen Inc. Procede et reactifs de detection de sequences d'acides nucleiques
GB8818020D0 (en) * 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
JPH04504356A (ja) * 1989-01-31 1992-08-06 ユニバーシティ オブ マイアミ 染色体ｄｎａの顕微解剖と増幅
EP0545459A1 (fr) * 1989-01-31 1993-06-09 The Regents Of The University Of California Amplification de l'ADN chromosomique trié par cytométrie
PL169035B1 (pl) * 1990-08-10 1996-05-31 Chiron Corp Sposób wykrywania sekwencji HCV PL
FR2678639B1 (fr) * 1991-07-03 1993-09-17 Rhone Poulenc Rorer Sa Procede de clonage d'acides nucleiques.
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
CA2318371A1 (fr) * 1998-01-13 1999-07-22 Biochip Technologies Gmbh Procede de detection de sequences nucleotidiques
DE10108626A1 (de) * 2001-02-22 2002-09-05 Sahin Ugur Verfahren zur Identifizierung biologisch aktiver Strukturen mikrobieller Erreger

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988010315A1 (fr) * 1987-06-19 1988-12-29 Siska Diagnostics, Inc. Systemes d'amplification/detection d'acides nucleiques a base de transcription
WO1989012695A1 (fr) * 1988-06-17 1989-12-28 Genelabs Incorporated Techniques d'amplification et de soustraction d'adn
WO1990001064A1 (fr) * 1988-07-26 1990-02-08 Genelabs Incorporated Techniques d'amplification selectve de sequences
WO1990001065A1 (fr) * 1988-07-26 1990-02-08 Genelabs Incorporated Techniques d'amplification d'adn et d'arn
EP0201184B1 (fr) * 1985-03-28 1992-12-16 F. Hoffmann-La Roche Ag Procédé pour l'amplification des séquences d'acide nucléique

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0201184B1 (fr) * 1985-03-28 1992-12-16 F. Hoffmann-La Roche Ag Procédé pour l'amplification des séquences d'acide nucléique
WO1988010315A1 (fr) * 1987-06-19 1988-12-29 Siska Diagnostics, Inc. Systemes d'amplification/detection d'acides nucleiques a base de transcription
WO1989012695A1 (fr) * 1988-06-17 1989-12-28 Genelabs Incorporated Techniques d'amplification et de soustraction d'adn
WO1990001064A1 (fr) * 1988-07-26 1990-02-08 Genelabs Incorporated Techniques d'amplification selectve de sequences
WO1990001065A1 (fr) * 1988-07-26 1990-02-08 Genelabs Incorporated Techniques d'amplification d'adn et d'arn

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Gene, Volume 34, No. 2/3, 1985, Elsevier Science Publishers, (Amsterdam, NL), C. COLECLOUGH et al.: "Use of Primer-Restriction-End Adapters in a Novel cDNA Cloning Strategy", pages 305-314 *
Nucleic Acids Research, Volume 14, No. 4, 1986, IRL Press Limited, (Oxford, GB), U. KRAWINKEL et al.: "Rapid Synthesis of cDNA for Cloning into Lambda Vectors", page 1913 *
Nucleic Acids Research, Volume 17, No. 8, April 1989, IRL Press, A. BELYAVSKY et al.: "PCR-Based cDNA Library Construction: General cDNA Libraries at the Level of a Few Cells", pages 2919-2932 see the whole article *
Proc. Natl. Acad. Sci. USA, Volume 85, December 1988, M.A. INNIS et al.: "DNA Sequencing with Thermus Aquaticus DNA Polymerase and Direct Sequencing of Polymerase Chain Reaction-Amplified DNA", pages 9436-9440 *
Science, Volume 243, 13 January 1989, E.Y. LOH et al.: "Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor & Chain", pages 217-218 *

Also Published As

Publication number Publication date
EP0458909A1 (fr) 1991-12-04
WO1990009457A2 (fr) 1990-08-23
CA2046919A1 (fr) 1990-08-15
AU5404890A (en) 1990-09-05

Similar Documents

Publication Publication Date Title
WO2002052031A3 (fr) Amplification d'acide nucleique
WO1993016094A3 (fr) Utilisation de n-nucleosides fluorescents et de leurs analogues structurels fluorescents
DK0487628T3 (da) Forbedret fremgangsmåde til nukleinsyre-amplifikation
ES2108748T3 (es) Amplificacion de acidos nucleicos con actividad de rna polimerasa dependiente de dna, de rna replicasas.
WO1997018334A3 (fr) Sondes d'acides nucleiques complementaires des acides nucleiques du virus du papillome humain, methodes et preparations associees
EP0329822A3 (en) Nucleic acid amplification process
WO2001013086A3 (fr) Detection d'acides nucleiques
IL148091A0 (en) Methods and compositions for linear isothermal amplification of polynucleotide sequences
HUT75993A (en) Methods and compositions for efficient nucleic acid sequencing
CA2172248A1 (fr) Detection et quantification d'une sequence d'acide nucleique par marquage electrochimioluminescent
AU7104300A (en) Method for amplifying signal-flanking sequences from unknown genomic dna
WO2002072773A3 (fr) Methodes et compositions pour amplification de sequences d'arn
AU6672601A (en) Cold sensitive mutant dna polymerases
EP0422869A3 (fr) Sondes d'acide nucléique et procédés pour la détection des levures Candida pathogènes
AU4391797A (en) The use of modular oligonucleotides as probes or primers in nucleic acid based assay
WO1998026093A3 (fr) Formation en epingle a cheveux d'analogue de nucleotides fluorescents pour la detection de l'hybridation d'acides nucleiques
EP0745675A3 (fr) Procédé pour l'amplification des séquences d'acides nucléiques et réactif utilisé dans ce but
ATE361377T1 (de) Leistungstarke verknüpfung von nukleinsäuresegmenten
WO1990009457A3 (fr) Paires d'amorce d'oligonucleotides pour l'amplification genetique independante de la sequence et leurs procedes d'utilisation
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
DE69518697D1 (de) Nachweis von Treponema Pallidum und Haemophilus Ducreyi
WO1998054313A3 (fr) Sequences genomiques d'adn methyltransferase et oligonucleotides antisens
WO1996015264A3 (fr) Procede d'identification de sequences nucleotidiques a partir de differentes sources biologiques par amplification des sequences
TW329476B (en) Method for detecting contaminant DNA in a protein product and kits used for the same purpose
WO1995017522A3 (fr) Identification de sequences repetees simples en tandem

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB IT LU NL SE

AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB IT LU NL SE

WWE Wipo information: entry into national phase

Ref document number: 2046919

Country of ref document: CA

Ref document number: 1990906003

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1990906003

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1990906003

Country of ref document: EP