AU2001268291A1 - Charge-switch nucleotides - Google Patents

Charge-switch nucleotides

Info

Publication number
AU2001268291A1
AU2001268291A1 AU2001268291A AU6829101A AU2001268291A1 AU 2001268291 A1 AU2001268291 A1 AU 2001268291A1 AU 2001268291 A AU2001268291 A AU 2001268291A AU 6829101 A AU6829101 A AU 6829101A AU 2001268291 A1 AU2001268291 A1 AU 2001268291A1
Authority
AU
Australia
Prior art keywords
charge
probe
phosphate
switch
intact
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001268291A
Other languages
English (en)
Inventor
Gregory R. Bashford
Jiyan Chen
Dan Draney
Nara Narayanan
Bambi L. Reynolds
Pamela Sheaff
John G.K. Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Li Cor Inc
Original Assignee
Li Cor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/876,375 external-priority patent/US6869764B2/en
Priority claimed from US09/876,374 external-priority patent/US6936702B2/en
Application filed by Li Cor Inc filed Critical Li Cor Inc
Publication of AU2001268291A1 publication Critical patent/AU2001268291A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
AU2001268291A 2000-06-07 2001-06-07 Charge-switch nucleotides Abandoned AU2001268291A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US20989600P 2000-06-07 2000-06-07
US60209896 2000-06-07
US28623801P 2001-04-24 2001-04-24
US60286238 2001-04-24
US09876374 2001-06-06
US09876375 2001-06-06
US09/876,375 US6869764B2 (en) 2000-06-07 2001-06-06 Nucleic acid sequencing using charge-switch nucleotides
US09/876,374 US6936702B2 (en) 2000-06-07 2001-06-06 Charge-switch nucleotides
PCT/US2001/018699 WO2001094609A1 (en) 2000-06-07 2001-06-07 Charge-switch nucleotides

Publications (1)

Publication Number Publication Date
AU2001268291A1 true AU2001268291A1 (en) 2001-12-17

Family

ID=27498783

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001268291A Abandoned AU2001268291A1 (en) 2000-06-07 2001-06-07 Charge-switch nucleotides

Country Status (5)

Country Link
EP (2) EP2226330B1 (de)
JP (1) JP2004516810A (de)
AU (1) AU2001268291A1 (de)
CA (1) CA2412567A1 (de)
WO (1) WO2001094609A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1354064A2 (de) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US7560254B2 (en) 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
GB0128526D0 (en) * 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides
DE10224339A1 (de) * 2002-05-29 2003-12-11 Axaron Bioscience Ag Verfahren zur hochparallelen Nukleinsäuresequenzierung
EP1546399B1 (de) * 2002-08-29 2012-02-29 GE Healthcare Bio-Sciences Corp. Nukleosidpolyphosphate mit blockiertem terminalem phosphat
AU2003268322B2 (en) * 2002-08-29 2009-07-16 Global Life Sciences Solutions Usa Llc Allele specific primer extension
EP1546354B1 (de) * 2002-08-29 2010-05-05 Amersham Biosciences Corp. Analytnachweis
JP4896707B2 (ja) * 2003-02-05 2012-03-14 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 固相配列決定法
JP2006513705A (ja) * 2003-02-05 2006-04-27 アマシャム・バイオサイエンス・コーポレイション 核酸増殖
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
CN104862383B (zh) 2008-03-28 2019-05-28 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
WO2012083249A2 (en) 2010-12-17 2012-06-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
CN102621214B (zh) * 2012-03-13 2014-10-29 美国哈佛大学 一种基于固态纳米孔对核酸分子进行减速及单分子捕获的方法
CN107082792A (zh) * 2012-04-09 2017-08-22 纽约哥伦比亚大学理事会 纳米孔的制备方法和其用途
US20150119259A1 (en) 2012-06-20 2015-04-30 Jingyue Ju Nucleic acid sequencing by nanopore detection of tag molecules
CN105102627B (zh) 2013-03-15 2018-10-19 纽约哥伦比亚大学理事会 用于检测样品中多种预定化合物的方法
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9863880B2 (en) 2013-11-17 2018-01-09 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
AU2015236392A1 (en) 2014-03-24 2016-10-20 Genia Technologies, Inc. Chemical methods for producing tagged nucleotides
MX2017001806A (es) 2014-08-08 2018-01-30 Quantum Si Inc Dispositivo integrado para el depósito temporal de fotones recibidos.
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
KR20220165282A (ko) 2014-08-08 2022-12-14 퀀텀-에스아이 인코포레이티드 분자들을 프로빙, 검출 및 분석하기 위한 광학계 및 검정 칩
AU2015300779B2 (en) 2014-08-08 2021-03-04 Quantum-Si Incorporated Integrated device with external light source for probing, detecting, and analyzing molecules
EP3315461B1 (de) * 2015-06-23 2021-07-07 BGI Shenzhen Mikroporöse elektrode und verfahren zur analyse chemischer substanzen
JP7287942B2 (ja) 2017-07-24 2023-06-06 クアンタム－エスアイ インコーポレイテッド 高強度標識反応物組成物および配列決定のための方法
BR112019025493A2 (pt) * 2018-02-16 2020-09-01 Illumina, Inc. nucleotídeos marcados e seus usos
CA3127102A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
CN113710686A (zh) 2019-02-19 2021-11-26 阿尔缇玛基因组学公司 用于光学检测和测序的接头和方法
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
CN115028547B (zh) * 2022-05-17 2023-09-12 浙江华伟医药科技有限公司 一种邻氨基苯甲酰胺的微通道化学合成方法
CN116375780B (zh) * 2023-06-02 2023-08-15 山东大学 一种用于识别gtp结合蛋白的光亲和探针及其制备方法和应用

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4973679A (en) 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US5260433A (en) * 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4739044A (en) 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4855225A (en) 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
FR2596761B1 (fr) 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US4793705A (en) 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
US4979824A (en) 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US6984487B1 (en) 1989-08-22 2006-01-10 Hsc Research Development Corporation Cystic fibrosis gene
US5578443A (en) 1991-03-06 1996-11-26 Regents Of The University Of Minnesota DNA sequence-based HLA typing method
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5681940A (en) 1994-11-02 1997-10-28 Icn Pharmaceuticals Sugar modified nucleosides and oligonucleotides
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
AU699939B2 (en) * 1995-06-23 1998-12-17 Baylor College Of Medicine Alternative dye-labeled primers, ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis and homogeneous amplification/detection assays
US5814524A (en) 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
GB9626815D0 (en) * 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6482938B1 (en) * 1997-07-11 2002-11-19 Wako Pure Chemical Industries, Ltd. Compounds having energy transfer function and method for DNA base sequencing by using the same
US6232075B1 (en) 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
US6323186B1 (en) * 1999-09-17 2001-11-27 Molecular Probes, Inc. Phosphate-bound polyazaindacene derivatives of nucleotides

Also Published As

Publication number Publication date
EP2226330A1 (de) 2010-09-08
JP2004516810A (ja) 2004-06-10
CA2412567A1 (en) 2001-12-13
EP1287154A1 (de) 2003-03-05
EP2226330B1 (de) 2013-09-18
EP1287154A4 (de) 2010-12-15
WO2001094609A1 (en) 2001-12-13

Similar Documents

Publication Publication Date Title
EP3112478B1 (de) Auf ladeschaltungsnukleotiden basierendes verfahren zur sequenzierung von nukleinsäuren
US6869764B2 (en) Nucleic acid sequencing using charge-switch nucleotides
EP2226330B1 (de) Ladeschaltungs-Nukleotide
US20020168678A1 (en) Flowcell system for nucleic acid sequencing
US7118907B2 (en) Single molecule detection systems and methods
JP4638043B2 (ja) ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法
AU2006297104A1 (en) Labeled nucleotide analogs and uses therefor
WO2002086088A2 (en) Polymerases with charge-switch activity and methods of generating such polymerases
JP2004516810A5 (de)

Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application