AU2001259672A1 - Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics - Google Patents

Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics

Info

Publication number
AU2001259672A1
AU2001259672A1 AU2001259672A AU5967201A AU2001259672A1 AU 2001259672 A1 AU2001259672 A1 AU 2001259672A1 AU 2001259672 A AU2001259672 A AU 2001259672A AU 5967201 A AU5967201 A AU 5967201A AU 2001259672 A1 AU2001259672 A1 AU 2001259672A1
Authority
AU
Australia
Prior art keywords
polynucleotides
identification
methods
desired characteristics
enzymatic assembly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001259672A
Other languages
English (en)
Inventor
Simon Delagrave
Barry Marrs
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hercules LLC
Original Assignee
Hercules LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hercules LLC filed Critical Hercules LLC
Publication of AU2001259672A1 publication Critical patent/AU2001259672A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2001259672A 2000-05-16 2001-05-10 Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics Abandoned AU2001259672A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/571,774 US6479262B1 (en) 2000-05-16 2000-05-16 Solid phase enzymatic assembly of polynucleotides
US09571774 2000-05-16
PCT/US2001/014966 WO2001088173A2 (fr) 2000-05-16 2001-05-10 Procedes d'assemblage enzymatique de polynucleotides et identification de polynucleotides possedant des caracteristiques souhaitees

Publications (1)

Publication Number Publication Date
AU2001259672A1 true AU2001259672A1 (en) 2001-11-26

Family

ID=24284992

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001259672A Abandoned AU2001259672A1 (en) 2000-05-16 2001-05-10 Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics

Country Status (5)

Country Link
US (2) US6479262B1 (fr)
EP (1) EP1282698A2 (fr)
AR (1) AR028454A1 (fr)
AU (1) AU2001259672A1 (fr)
WO (1) WO2001088173A2 (fr)

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US20030049619A1 (en) * 2001-03-21 2003-03-13 Simon Delagrave Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides
EP1538980B1 (fr) * 2002-06-25 2017-01-18 Ultrashape Ltd. Dispositif pour l'esthetique corporelle
NL1022537C2 (nl) * 2003-01-31 2004-08-03 Univ Delft Tech Werkwijze voor de bereiding van chimere polynucleotiden.
WO2008027558A2 (fr) 2006-08-31 2008-03-06 Codon Devices, Inc. Assemblage itératif d'acides nucléiques utilisant l'activation de caractères codés par vecteurs
CA2723242A1 (fr) 2008-05-14 2009-11-19 British Columbia Cancer Agency Branch Synthese de genes par assemblage convergent de sous-ensembles d'oligonucleotides
WO2010025310A2 (fr) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Méthodes et dispositifs de synthèse de polynucléotides haute fidélité
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
EP3653763A1 (fr) 2009-02-13 2020-05-20 X-Chem, Inc. Procédés de création et de criblage de bibliothèques de codage adn
WO2011056872A2 (fr) 2009-11-03 2011-05-12 Gen9, Inc. Procédés et dispositifs microfluidiques pour la manipulation de gouttelettes dans un ensemble polynucléotidique haute fidélité
EP3597771A1 (fr) 2009-11-25 2020-01-22 Gen9, Inc. Procédés et appareils permettant la réduction des erreurs de l'adn basée sur une puce
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (fr) 2010-01-07 2011-07-14 Gen9, Inc. Assemblage de polynucléotides haute fidélité
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
WO2011150168A1 (fr) 2010-05-28 2011-12-01 Gen9, Inc. Procédés et dispositifs de synthèse in situ d'acides nucléiques
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
EP3360963B1 (fr) 2010-11-12 2019-11-06 Gen9, Inc. Procédés et dispositifs pour la synthèse d'acides nucléiques
EP2637780B1 (fr) 2010-11-12 2022-02-09 Gen9, Inc. Puces à protéines et leurs procédés d'utilisation et de fabrication
EP2665833B1 (fr) 2011-01-17 2017-04-19 Life Technologies Corporation Workflow pour la détection de ligands à l'aide d'acides nucléiques
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
CN102876658A (zh) * 2011-07-12 2013-01-16 北京唯尚立德生物科技有限公司 一种大规模合成长链核酸分子的方法
LT3594340T (lt) 2011-08-26 2021-10-25 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
KR102124613B1 (ko) * 2011-09-07 2020-06-19 엑스-켐, 인크. Dna-코딩된 라이브러리에 태그부착하는 방법
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP2841601B1 (fr) 2012-04-24 2019-03-06 Gen9, Inc. Procédés de tri d'acides nucléiques et de clonage in vitro multiplex préparatoire
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
DK2872680T3 (en) 2012-07-13 2018-07-09 X Chem Inc DNA-encoded libraries with coding oligonucleotide compounds that cannot be read with polymerases
EP3164489B1 (fr) * 2014-07-03 2020-05-13 Rhodx, Inc. Étiquetage et évaluation d'une séquence cible
GB2559117B (en) * 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
EP3737755A1 (fr) * 2018-01-12 2020-11-18 Camena Bioscience Limited Compositions et procédés de synthèse d'acides nucléiques enzymatique géométrique sans matrice
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
CN115109813A (zh) * 2022-02-09 2022-09-27 康龙化成(宁波)科技发展有限公司 一种一锅法实现5`-磷酸化以及寡聚核苷酸偶联的合成方法及应用

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US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
GB2123428B (en) * 1982-02-01 1986-02-05 Innovax Lab Ltd Method for production of predetermined polyribonucleotides
JPS61152695A (ja) 1984-12-26 1986-07-11 Nippon Shinyaku Co Ltd 長鎖ｄｎａの合成法
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
JP2584613B2 (ja) 1985-03-30 1997-02-26 バリベ、マール 組換えｄｎａ技術によってｄｎａ、ｒｎａ、ペプタイド、ポリペプタイド、または蛋白質を取得するための方法
AU3869289A (en) 1988-07-14 1990-02-05 Baylor College Of Medicine Solid phase assembly and reconstruction of biopolymers
US5262530A (en) 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
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WO1993019202A2 (fr) 1992-03-10 1993-09-30 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Reaction a matrice echangeable
US5516664A (en) 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
DE19633427C2 (de) 1996-08-20 2001-10-04 Hubert S Bernauer Verfahren zur Synthese von Nukleinsäuremolekülen mit zumindest teilweise vorbestimmter Nukleotidsequenz
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
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DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
US5914245A (en) 1998-04-20 1999-06-22 Kairos Scientific Inc. Solid phase enzyme kinetics screening in microcolonies
CA2335562A1 (fr) 1998-06-22 1999-12-29 Roxana Maria Havlina Production de polymeres par voie enzymatique a partir d'elements nucleotidiques et/ou non nucleotidiques
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
JP2004500019A (ja) 1999-03-26 2004-01-08 ディベルサ　コーポレーション 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合
US20010053849A1 (en) 1999-06-16 2001-12-20 Mary Jeanne Kreek Plural biological sample arrays, and preparation and uses thereof

Also Published As

Publication number Publication date
US20030082536A1 (en) 2003-05-01
EP1282698A2 (fr) 2003-02-12
WO2001088173A3 (fr) 2002-06-06
US6635453B2 (en) 2003-10-21
WO2001088173A2 (fr) 2001-11-22
AR028454A1 (es) 2003-05-07
US6479262B1 (en) 2002-11-12

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