AR028454A1 - Metodos para el ensamblaje enzimatico de polinucleotidos e identificacion de polinucleotidos que tienen caracteristicas deseadas - Google Patents

Metodos para el ensamblaje enzimatico de polinucleotidos e identificacion de polinucleotidos que tienen caracteristicas deseadas

Info

Publication number
AR028454A1
AR028454A1 ARP010102275A ARP010102275A AR028454A1 AR 028454 A1 AR028454 A1 AR 028454A1 AR P010102275 A ARP010102275 A AR P010102275A AR P010102275 A ARP010102275 A AR P010102275A AR 028454 A1 AR028454 A1 AR 028454A1
Authority
AR
Argentina
Prior art keywords
polinucleotides
polynucleotides
identification
methods
desired characteristics
Prior art date
Application number
ARP010102275A
Other languages
English (en)
Original Assignee
Hercules Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hercules Inc filed Critical Hercules Inc
Publication of AR028454A1 publication Critical patent/AR028454A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07BGENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11Compounds covalently bound to a solid support
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
ARP010102275A 2000-05-16 2001-05-14 Metodos para el ensamblaje enzimatico de polinucleotidos e identificacion de polinucleotidos que tienen caracteristicas deseadas AR028454A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/571,774 US6479262B1 (en) 2000-05-16 2000-05-16 Solid phase enzymatic assembly of polynucleotides

Publications (1)

Publication Number Publication Date
AR028454A1 true AR028454A1 (es) 2003-05-07

Family

ID=24284992

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010102275A AR028454A1 (es) 2000-05-16 2001-05-14 Metodos para el ensamblaje enzimatico de polinucleotidos e identificacion de polinucleotidos que tienen caracteristicas deseadas

Country Status (5)

Country Link
US (2) US6479262B1 (es)
EP (1) EP1282698A2 (es)
AR (1) AR028454A1 (es)
AU (1) AU2001259672A1 (es)
WO (1) WO2001088173A2 (es)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030049619A1 (en) * 2001-03-21 2003-03-13 Simon Delagrave Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides
EP1538980B1 (en) * 2002-06-25 2017-01-18 Ultrashape Ltd. Device for body aesthetics
NL1022537C2 (nl) * 2003-01-31 2004-08-03 Univ Delft Tech Werkwijze voor de bereiding van chimere polynucleotiden.
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
CA2723242A1 (en) 2008-05-14 2009-11-19 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
EP3653763A1 (en) 2009-02-13 2020-05-20 X-Chem, Inc. Methods of creating and screening dna-encoded libraries
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
EP3597771A1 (en) 2009-11-25 2020-01-22 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
EP3360963B1 (en) 2010-11-12 2019-11-06 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
EP2665833B1 (en) 2011-01-17 2017-04-19 Life Technologies Corporation Workflow for detection of ligands using nucleic acids
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
CN102876658A (zh) * 2011-07-12 2013-01-16 北京唯尚立德生物科技有限公司 一种大规模合成长链核酸分子的方法
LT3594340T (lt) 2011-08-26 2021-10-25 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
KR102124613B1 (ko) * 2011-09-07 2020-06-19 엑스-켐, 인크. Dna-코딩된 라이브러리에 태그부착하는 방법
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP2841601B1 (en) 2012-04-24 2019-03-06 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
DK2872680T3 (en) 2012-07-13 2018-07-09 X Chem Inc DNA-encoded libraries with coding oligonucleotide compounds that cannot be read with polymerases
EP3164489B1 (en) * 2014-07-03 2020-05-13 Rhodx, Inc. Tagging and assessing a target sequence
GB2559117B (en) * 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
EP3737755A1 (en) * 2018-01-12 2020-11-18 Camena Bioscience Limited Compositions and methods for template-free geometric enzymatic nucleic acid synthesis
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
CN115109813A (zh) * 2022-02-09 2022-09-27 康龙化成(宁波)科技发展有限公司 一种一锅法实现5`-磷酸化以及寡聚核苷酸偶联的合成方法及应用

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
GB2123428B (en) * 1982-02-01 1986-02-05 Innovax Lab Ltd Method for production of predetermined polyribonucleotides
JPS61152695A (ja) 1984-12-26 1986-07-11 Nippon Shinyaku Co Ltd 長鎖dnaの合成法
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
JP2584613B2 (ja) 1985-03-30 1997-02-26 バリベ、マール 組換えdna技術によってdna、rna、ペプタイド、ポリペプタイド、または蛋白質を取得するための方法
AU3869289A (en) 1988-07-14 1990-02-05 Baylor College Of Medicine Solid phase assembly and reconstruction of biopolymers
US5262530A (en) 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
EP0406937A3 (en) 1989-06-30 1992-01-22 Eastman Kodak Company In vitro gene synthesis
WO1993019202A2 (en) 1992-03-10 1993-09-30 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Exchangeable template reaction
US5516664A (en) 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
DE19633427C2 (de) 1996-08-20 2001-10-04 Hubert S Bernauer Verfahren zur Synthese von Nukleinsäuremolekülen mit zumindest teilweise vorbestimmter Nukleotidsequenz
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6521427B1 (en) 1997-09-16 2003-02-18 Egea Biosciences, Inc. Method for the complete chemical synthesis and assembly of genes and genomes
DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
US5914245A (en) 1998-04-20 1999-06-22 Kairos Scientific Inc. Solid phase enzyme kinetics screening in microcolonies
CA2335562A1 (en) 1998-06-22 1999-12-29 Roxana Maria Havlina Production of polymers from nucleotidic and/or non-nucleotidic elements using enzymatic methods
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
JP2004500019A (ja) 1999-03-26 2004-01-08 ディベルサ コーポレーション 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合
US20010053849A1 (en) 1999-06-16 2001-12-20 Mary Jeanne Kreek Plural biological sample arrays, and preparation and uses thereof

Also Published As

Publication number Publication date
US20030082536A1 (en) 2003-05-01
EP1282698A2 (en) 2003-02-12
WO2001088173A3 (en) 2002-06-06
US6635453B2 (en) 2003-10-21
AU2001259672A1 (en) 2001-11-26
WO2001088173A2 (en) 2001-11-22
US6479262B1 (en) 2002-11-12

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