AU2001248599A1 - Type ii gonadotropin-releasing hormone receptor and polynucleotides encoding therefor - Google Patents
Type ii gonadotropin-releasing hormone receptor and polynucleotides encoding thereforInfo
- Publication number
- AU2001248599A1 AU2001248599A1 AU2001248599A AU4859901A AU2001248599A1 AU 2001248599 A1 AU2001248599 A1 AU 2001248599A1 AU 2001248599 A AU2001248599 A AU 2001248599A AU 4859901 A AU4859901 A AU 4859901A AU 2001248599 A1 AU2001248599 A1 AU 2001248599A1
- Authority
- AU
- Australia
- Prior art keywords
- gnrh
- type
- receptor
- peptide
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000002157 polynucleotide Substances 0.000 title claims description 29
- 102000040430 polynucleotide Human genes 0.000 title claims description 27
- 108091033319 polynucleotide Proteins 0.000 title claims description 27
- 108010021290 LHRH Receptors Proteins 0.000 title claims description 19
- 102000008238 LHRH Receptors Human genes 0.000 title claims description 9
- 102100033851 Gonadotropin-releasing hormone receptor Human genes 0.000 claims description 105
- 101710082444 Gonadotropin-releasing hormone receptor Proteins 0.000 claims description 95
- 102000005962 receptors Human genes 0.000 claims description 93
- 108020003175 receptors Proteins 0.000 claims description 93
- 241000282414 Homo sapiens Species 0.000 claims description 59
- 101000857870 Squalus acanthias Gonadoliberin Proteins 0.000 claims description 51
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 49
- 241001515942 marmosets Species 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 17
- 239000002773 nucleotide Substances 0.000 claims description 17
- 125000003729 nucleotide group Chemical group 0.000 claims description 17
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- NMJREATYWWNIKX-UHFFFAOYSA-N GnRH Chemical compound C1CCC(C(=O)NCC(N)=O)N1C(=O)C(CC(C)C)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)CNC(=O)C(NC(=O)C(CO)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C(CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 NMJREATYWWNIKX-UHFFFAOYSA-N 0.000 claims description 12
- 241001465754 Metazoa Species 0.000 claims description 7
- 238000001727 in vivo Methods 0.000 claims description 7
- 230000019491 signal transduction Effects 0.000 claims description 6
- 239000013598 vector Substances 0.000 claims description 6
- 230000009261 transgenic effect Effects 0.000 claims description 5
- 230000002068 genetic effect Effects 0.000 claims description 4
- 241000288906 Primates Species 0.000 claims description 3
- 230000000144 pharmacologic effect Effects 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 239000003433 contraceptive agent Substances 0.000 claims description 2
- 239000013604 expression vector Substances 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 230000002254 contraceptive effect Effects 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical compound C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 description 72
- 101800000330 Gonadoliberin II Proteins 0.000 description 49
- 101800000476 Gonadoliberin-2 Proteins 0.000 description 49
- 102400001226 Gonadoliberin-2 Human genes 0.000 description 48
- 230000014509 gene expression Effects 0.000 description 43
- 210000004027 cell Anatomy 0.000 description 42
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 description 39
- 229940035638 gonadotropin-releasing hormone Drugs 0.000 description 39
- 101800000477 Gonadoliberin-1 Proteins 0.000 description 35
- 101710118786 Gonadotropin-releasing hormone II receptor Proteins 0.000 description 35
- 102100033845 Putative gonadotropin-releasing hormone II receptor Human genes 0.000 description 35
- 150000001413 amino acids Chemical group 0.000 description 29
- 210000001519 tissue Anatomy 0.000 description 26
- 102000009151 Luteinizing Hormone Human genes 0.000 description 25
- 108010073521 Luteinizing Hormone Proteins 0.000 description 25
- 229940040129 luteinizing hormone Drugs 0.000 description 25
- 108090000623 proteins and genes Proteins 0.000 description 24
- 230000000638 stimulation Effects 0.000 description 20
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 19
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 19
- 229940028334 follicle stimulating hormone Drugs 0.000 description 19
- 230000028327 secretion Effects 0.000 description 19
- 239000002299 complementary DNA Substances 0.000 description 17
- 230000001817 pituitary effect Effects 0.000 description 17
- 235000001014 amino acid Nutrition 0.000 description 15
- 102000004196 processed proteins & peptides Human genes 0.000 description 15
- 239000000047 product Substances 0.000 description 13
- 230000004913 activation Effects 0.000 description 12
- 210000004556 brain Anatomy 0.000 description 12
- 230000000694 effects Effects 0.000 description 12
- 108091054455 MAP kinase family Proteins 0.000 description 11
- 102000043136 MAP kinase family Human genes 0.000 description 11
- 102000004389 Ribonucleoproteins Human genes 0.000 description 11
- 108010081734 Ribonucleoproteins Proteins 0.000 description 11
- 239000005557 antagonist Substances 0.000 description 11
- 239000003446 ligand Substances 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- 108020004705 Codon Proteins 0.000 description 9
- 108091060211 Expressed sequence tag Proteins 0.000 description 9
- 102000006771 Gonadotropins Human genes 0.000 description 9
- 108010086677 Gonadotropins Proteins 0.000 description 9
- 241001494479 Pecora Species 0.000 description 9
- 239000000556 agonist Substances 0.000 description 9
- 230000006870 function Effects 0.000 description 9
- 239000002622 gonadotropin Substances 0.000 description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- 102100024193 Mitogen-activated protein kinase 1 Human genes 0.000 description 8
- INAPMGSXUVUWAF-GCVPSNMTSA-N [(2r,3s,5r,6r)-2,3,4,5,6-pentahydroxycyclohexyl] dihydrogen phosphate Chemical compound OC1[C@H](O)[C@@H](O)C(OP(O)(O)=O)[C@H](O)[C@@H]1O INAPMGSXUVUWAF-GCVPSNMTSA-N 0.000 description 8
- 230000000692 anti-sense effect Effects 0.000 description 8
- 210000002569 neuron Anatomy 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 230000004044 response Effects 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 108020004999 messenger RNA Proteins 0.000 description 7
- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 6
- FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 6
- 101100291385 Drosophila melanogaster p38a gene Proteins 0.000 description 6
- 101150110792 GNRHR gene Proteins 0.000 description 6
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 6
- 229940094892 gonadotropins Drugs 0.000 description 6
- 210000003016 hypothalamus Anatomy 0.000 description 6
- 230000001404 mediated effect Effects 0.000 description 6
- 230000001850 reproductive effect Effects 0.000 description 6
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 6
- 229940055619 selenocysteine Drugs 0.000 description 6
- 235000016491 selenocysteine Nutrition 0.000 description 6
- 241000282693 Cercopithecidae Species 0.000 description 5
- 108091026890 Coding region Proteins 0.000 description 5
- 101000876610 Dictyostelium discoideum Extracellular signal-regulated kinase 2 Proteins 0.000 description 5
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 description 5
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 description 5
- 101001052493 Homo sapiens Mitogen-activated protein kinase 1 Proteins 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 210000002307 prostate Anatomy 0.000 description 5
- 210000002637 putamen Anatomy 0.000 description 5
- NMJREATYWWNIKX-XJIZABAQSA-N (2s)-n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[2-[[(2s)-1-[[(2s)-1-[(2s)-2-[(2-amino-2-oxoethyl)carbamoyl]pyrrolidin-1-yl]-4-methyl-1-oxopentan-2-yl]amino]-3-(1h-indol-3-yl)-1-oxopropan-2-yl]amino]-2-oxoethyl]amino]-3-(4-hydroxyphenyl)-1-oxopropan-2-yl]amino Chemical compound C([C@@H](C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 NMJREATYWWNIKX-XJIZABAQSA-N 0.000 description 4
- 102000007469 Actins Human genes 0.000 description 4
- 108010085238 Actins Proteins 0.000 description 4
- 108010059616 Activins Proteins 0.000 description 4
- 108010055717 JNK Mitogen-Activated Protein Kinases Proteins 0.000 description 4
- 125000000570 L-alpha-aspartyl group Chemical group [H]OC(=O)C([H])([H])[C@]([H])(N([H])[H])C(*)=O 0.000 description 4
- 241000282560 Macaca mulatta Species 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 238000000636 Northern blotting Methods 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 108010047495 alanylglycine Proteins 0.000 description 4
- 210000004727 amygdala Anatomy 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 210000004940 nucleus Anatomy 0.000 description 4
- 230000036961 partial effect Effects 0.000 description 4
- 210000003814 preoptic area Anatomy 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 230000010539 reproductive behavior Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 210000000331 sympathetic ganglia Anatomy 0.000 description 4
- 108020005345 3' Untranslated Regions Proteins 0.000 description 3
- 102000005606 Activins Human genes 0.000 description 3
- 108700024394 Exon Proteins 0.000 description 3
- 108010007457 Extracellular Signal-Regulated MAP Kinases Proteins 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 3
- GGEJHJIXRBTJPD-BYPYZUCNSA-N Gly-Asn-Gly Chemical compound NCC(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O GGEJHJIXRBTJPD-BYPYZUCNSA-N 0.000 description 3
- 125000000010 L-asparaginyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C(=O)N([H])[H] 0.000 description 3
- 241000270322 Lepidosauria Species 0.000 description 3
- CIDICGYKRUTYLE-FXQIFTODSA-N Met-Ser-Ala Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O CIDICGYKRUTYLE-FXQIFTODSA-N 0.000 description 3
- 241001529936 Murinae Species 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 241000270934 Rana catesbeiana Species 0.000 description 3
- 241000700159 Rattus Species 0.000 description 3
- IWAVRIPRTCJAQO-HSHDSVGOSA-N Thr-Pro-Trp Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O IWAVRIPRTCJAQO-HSHDSVGOSA-N 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 230000006399 behavior Effects 0.000 description 3
- 210000003169 central nervous system Anatomy 0.000 description 3
- 210000001638 cerebellum Anatomy 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 210000000877 corpus callosum Anatomy 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 210000001652 frontal lobe Anatomy 0.000 description 3
- 108010089804 glycyl-threonine Proteins 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 238000003365 immunocytochemistry Methods 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 210000005075 mammary gland Anatomy 0.000 description 3
- 210000001259 mesencephalon Anatomy 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 230000003227 neuromodulating effect Effects 0.000 description 3
- 210000001428 peripheral nervous system Anatomy 0.000 description 3
- 210000002826 placenta Anatomy 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 238000012552 review Methods 0.000 description 3
- 230000001568 sexual effect Effects 0.000 description 3
- 210000002027 skeletal muscle Anatomy 0.000 description 3
- 210000000278 spinal cord Anatomy 0.000 description 3
- 210000003478 temporal lobe Anatomy 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 2
- 108091006027 G proteins Proteins 0.000 description 2
- 102000030782 GTP binding Human genes 0.000 description 2
- 108091000058 GTP-Binding Proteins 0.000 description 2
- 101000996738 Gallus gallus Gonadoliberin-2 Proteins 0.000 description 2
- 101000996727 Homo sapiens Gonadotropin-releasing hormone receptor Proteins 0.000 description 2
- 101000668140 Homo sapiens RNA-binding protein 8A Proteins 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 102100039691 RNA-binding protein 8A Human genes 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 241000269370 Xenopus <genus> Species 0.000 description 2
- 230000001919 adrenal effect Effects 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000001028 anti-proliverative effect Effects 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 230000037007 arousal Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000000133 brain stem Anatomy 0.000 description 2
- 210000005013 brain tissue Anatomy 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 210000001159 caudate nucleus Anatomy 0.000 description 2
- 210000003710 cerebral cortex Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000004624 confocal microscopy Methods 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 230000002964 excitative effect Effects 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000001215 fluorescent labelling Methods 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 210000002149 gonad Anatomy 0.000 description 2
- 239000003163 gonadal steroid hormone Substances 0.000 description 2
- 210000001320 hippocampus Anatomy 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 210000001073 mediodorsal thalamic nucleus Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 210000000653 nervous system Anatomy 0.000 description 2
- 239000002858 neurotransmitter agent Substances 0.000 description 2
- 230000002611 ovarian Effects 0.000 description 2
- 108010068338 p38 Mitogen-Activated Protein Kinases Proteins 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 210000003635 pituitary gland Anatomy 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- 230000011664 signaling Effects 0.000 description 2
- 210000003523 substantia nigra Anatomy 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 210000004281 subthalamic nucleus Anatomy 0.000 description 2
- 210000004377 supraoptic nucleus Anatomy 0.000 description 2
- 210000001103 thalamus Anatomy 0.000 description 2
- 230000036962 time dependent Effects 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- NCYCYZXNIZJOKI-IOUUIBBYSA-N 11-cis-retinal Chemical compound O=C/C=C(\C)/C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-IOUUIBBYSA-N 0.000 description 1
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- RLMISHABBKUNFO-WHFBIAKZSA-N Ala-Ala-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)NCC(O)=O RLMISHABBKUNFO-WHFBIAKZSA-N 0.000 description 1
- BEMGNWZECGIJOI-WDSKDSINSA-N Ala-Gly-Glu Chemical compound [H]N[C@@H](C)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(O)=O BEMGNWZECGIJOI-WDSKDSINSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 108020000948 Antisense Oligonucleotides Proteins 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 101100007328 Cocos nucifera COS-1 gene Proteins 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 241000557626 Corvus corax Species 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 102000016970 Follistatin Human genes 0.000 description 1
- 108010014612 Follistatin Proteins 0.000 description 1
- 101500028367 Gallus gallus Gonadoliberin-1 Proteins 0.000 description 1
- QJCKNLPMTPXXEM-AUTRQRHGSA-N Glu-Glu-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CCC(O)=O QJCKNLPMTPXXEM-AUTRQRHGSA-N 0.000 description 1
- RAUDKMVXNOWDLS-WDSKDSINSA-N Glu-Gly-Ser Chemical compound OC(=O)CC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O RAUDKMVXNOWDLS-WDSKDSINSA-N 0.000 description 1
- XIJOPMSILDNVNJ-ZVZYQTTQSA-N Glu-Val-Trp Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O XIJOPMSILDNVNJ-ZVZYQTTQSA-N 0.000 description 1
- 241001326189 Gyrodactylus prostae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101001018097 Homo sapiens L-selectin Proteins 0.000 description 1
- 101000904173 Homo sapiens Progonadoliberin-1 Proteins 0.000 description 1
- 206010062767 Hypophysitis Diseases 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 108010004250 Inhibins Proteins 0.000 description 1
- 102000002746 Inhibins Human genes 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 102100033467 L-selectin Human genes 0.000 description 1
- LZDNBBYBDGBADK-UHFFFAOYSA-N L-valyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)C(C)C)C(O)=O)=CNC2=C1 LZDNBBYBDGBADK-UHFFFAOYSA-N 0.000 description 1
- 239000012741 Laemmli sample buffer Substances 0.000 description 1
- 241000179388 Melospiza Species 0.000 description 1
- YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 1
- 108010002311 N-glycylglutamic acid Proteins 0.000 description 1
- 108010066427 N-valyltryptophan Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 244000028344 Primula vulgaris Species 0.000 description 1
- 235000016311 Primula vulgaris Nutrition 0.000 description 1
- 102100024028 Progonadoliberin-1 Human genes 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 102100040756 Rhodopsin Human genes 0.000 description 1
- 108090000820 Rhodopsin Proteins 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 239000012163 TRI reagent Substances 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- UEHRGZCNLSWGHK-DLOVCJGASA-N Val-Glu-Val Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O UEHRGZCNLSWGHK-DLOVCJGASA-N 0.000 description 1
- SXEHKFHPFVVDIR-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine Chemical compound C1=CC(NN)=CC=C1C1=CC=C(NN)C=C1 SXEHKFHPFVVDIR-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000000488 activin Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000003098 androgen Substances 0.000 description 1
- 210000004198 anterior pituitary gland Anatomy 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000000074 antisense oligonucleotide Substances 0.000 description 1
- 238000012230 antisense oligonucleotides Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008436 biogenesis Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- 230000014107 chromosome localization Effects 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229940124558 contraceptive agent Drugs 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 229940095074 cyclic amp Drugs 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000000586 desensitisation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000012137 double-staining Methods 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 230000007783 downstream signaling Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000000918 epididymis Anatomy 0.000 description 1
- 201000010063 epididymitis Diseases 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 230000006543 gametophyte development Effects 0.000 description 1
- 230000004545 gene duplication Effects 0.000 description 1
- 108010055341 glutamyl-glutamic acid Proteins 0.000 description 1
- 108010035313 glycerophosphoinositol glycerophosphodiesterase Proteins 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 239000002474 gonadorelin antagonist Substances 0.000 description 1
- 210000004368 gonadotroph Anatomy 0.000 description 1
- 229940121381 gonadotrophin releasing hormone (gnrh) antagonists Drugs 0.000 description 1
- 230000002267 hypothalamic effect Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 239000012133 immunoprecipitate Substances 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 239000012073 inactive phase Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 239000000893 inhibin Substances 0.000 description 1
- ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000004068 intracellular signaling Effects 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 1
- 238000000021 kinase assay Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000001592 luteinising effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000028161 membrane depolarization Effects 0.000 description 1
- 230000000897 modulatory effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 230000003988 neural development Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 230000000955 neuroendocrine Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 210000002975 pon Anatomy 0.000 description 1
- 230000001242 postsynaptic effect Effects 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000026234 pro-estrus Effects 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 230000000541 pulsatile effect Effects 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000002464 receptor antagonist Substances 0.000 description 1
- 229940044551 receptor antagonist Drugs 0.000 description 1
- 238000001525 receptor binding assay Methods 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000003578 releasing effect Effects 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 210000001625 seminal vesicle Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000009329 sexual behaviour Effects 0.000 description 1
- 230000037432 silent mutation Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 210000004057 substantia innominata Anatomy 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 210000001685 thyroid gland Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000003146 transient transfection Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000003932 urinary bladder Anatomy 0.000 description 1
- 201000010653 vesiculitis Diseases 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000007794 visualization technique Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
- C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/16—Masculine contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/18—Feminine contraceptives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Endocrinology (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Gynecology & Obstetrics (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0009269A GB0009269D0 (en) | 2000-04-15 | 2000-04-15 | Receptor |
| GB0009269.2 | 2000-04-15 | ||
| GB0014761.1 | 2000-06-17 | ||
| GB0014761A GB0014761D0 (en) | 2000-06-17 | 2000-06-17 | Receptor |
| US21523200P | 2000-06-30 | 2000-06-30 | |
| US60215232 | 2000-06-30 | ||
| PCT/GB2001/001755 WO2001078796A1 (fr) | 2000-04-15 | 2001-04-17 | Recepteur de la gonadoliberine de type ii et polynucleotides codant pour ce recepteur |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001248599A1 true AU2001248599A1 (en) | 2001-10-30 |
Family
ID=27255670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001248599A Abandoned AU2001248599A1 (en) | 2000-04-15 | 2001-04-17 | Type ii gonadotropin-releasing hormone receptor and polynucleotides encoding therefor |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1337282A2 (fr) |
| JP (1) | JP2003530837A (fr) |
| KR (1) | KR20020097218A (fr) |
| AU (1) | AU2001248599A1 (fr) |
| BR (1) | BR0110098A (fr) |
| CA (1) | CA2404127A1 (fr) |
| HU (1) | HUP0301666A2 (fr) |
| IL (1) | IL152011A0 (fr) |
| NO (1) | NO20024937L (fr) |
| PL (1) | PL357684A1 (fr) |
| WO (1) | WO2001078796A1 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001281882A1 (en) * | 2000-06-26 | 2002-01-08 | Bayer Aktiengesellschaft | Regulation of human gonadotropin releasing hormone receptor-like g protein-coupled receptor |
| WO2002070701A2 (fr) * | 2001-03-01 | 2002-09-12 | Societe De Conseils De Recherches Et D'applications Scientifiques (S.C.R.A.S.) | Nouveau recepteur gnrh humain |
| US20050282745A1 (en) * | 2001-08-28 | 2005-12-22 | Siler-Khodr Theresa M | Non-mammalian GnRH analogs and uses thereof in regulation of fertility and pregnancy |
| US7361633B2 (en) | 2002-07-22 | 2008-04-22 | Ardana Bioscience Limited | Screening method and anti-tumor drug candidate obtained therefrom |
| WO2004010145A2 (fr) * | 2002-07-22 | 2004-01-29 | Ardana Bioscience Limited | Procede de criblage |
| WO2004009636A2 (fr) * | 2002-07-22 | 2004-01-29 | Ardana Bioscience Limited | Modulateur de la gnrh |
| KR101038183B1 (ko) * | 2010-12-27 | 2011-06-01 | 강범준 | 조립식 파형블록과 이를 이용한 호안 경사면 피복방법 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0647275T3 (da) * | 1992-06-23 | 2005-01-24 | Sinai School Medicine | Kloning og ekspression af receptoren for gonatotropin-frigörende hormon |
| AU3388597A (en) * | 1996-06-13 | 1998-01-07 | University Of Cape Town | Human type ii gonadotropin-releasing hormone receptor |
-
2001
- 2001-04-17 PL PL01357684A patent/PL357684A1/xx unknown
- 2001-04-17 WO PCT/GB2001/001755 patent/WO2001078796A1/fr not_active Application Discontinuation
- 2001-04-17 IL IL15201101A patent/IL152011A0/xx unknown
- 2001-04-17 HU HU0301666A patent/HUP0301666A2/hu unknown
- 2001-04-17 BR BR0110098-0A patent/BR0110098A/pt not_active Application Discontinuation
- 2001-04-17 EP EP01921629A patent/EP1337282A2/fr not_active Withdrawn
- 2001-04-17 AU AU2001248599A patent/AU2001248599A1/en not_active Abandoned
- 2001-04-17 KR KR1020027013776A patent/KR20020097218A/ko not_active Application Discontinuation
- 2001-04-17 CA CA002404127A patent/CA2404127A1/fr not_active Abandoned
- 2001-04-17 JP JP2001576095A patent/JP2003530837A/ja active Pending
-
2002
- 2002-10-14 NO NO20024937A patent/NO20024937L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| PL357684A1 (en) | 2004-07-26 |
| NO20024937L (no) | 2002-12-09 |
| WO2001078796A9 (fr) | 2002-02-28 |
| BR0110098A (pt) | 2003-01-14 |
| HUP0301666A2 (hu) | 2003-08-28 |
| EP1337282A2 (fr) | 2003-08-27 |
| KR20020097218A (ko) | 2002-12-31 |
| CA2404127A1 (fr) | 2001-10-25 |
| NO20024937D0 (no) | 2002-10-14 |
| WO2001078796A3 (fr) | 2003-04-24 |
| IL152011A0 (en) | 2003-04-10 |
| WO2001078796A1 (fr) | 2001-10-25 |
| JP2003530837A (ja) | 2003-10-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Millar et al. | A novel mammalian receptor for the evolutionarily conserved type II GnRH | |
| Reinhart et al. | Molecular cloning and expression of cDNA encoding the murine gonadotropin-releasing hormone receptor. | |
| Gallo-Payet et al. | Regulation of adrenocortical function by vasopressin | |
| JP4472256B2 (ja) | ウロコルチンｉｉｉ及びその利用 | |
| KR20020006720A (ko) | 이황화결합으로 연결된 당단백질 호르몬 유사체, 이의제조방법 및 이의 용도 | |
| AU2001248599A1 (en) | Type ii gonadotropin-releasing hormone receptor and polynucleotides encoding therefor | |
| RODRIGUEZ et al. | The orientation of the lutropin/choriogonadotropin receptor in rat luteal cells as revealed by site-specific antibodies | |
| US6927041B2 (en) | Human neuropeptide Y-like G protein-coupled receptor | |
| US6495343B1 (en) | Cloning and recombinant production of CRF receptor(s) | |
| US20030124585A1 (en) | Type II gonadotropin-releasing hormone receptor and polynucleotides encoding therefor | |
| US6482608B1 (en) | Cloning and recombinant production of CRF receptor(S) | |
| AU689119B2 (en) | Cloning and recombinant production of CRF (corticotropin releasing factor) receptor(s) | |
| DE69930873T2 (de) | Dna-moleküle kodierende spleissvarianten des humanen melanocortin-1-rezeptorproteins | |
| US7666994B2 (en) | Cloning and expression of gonadotropin-releasing hormone (GnRH) receptors | |
| US5910428A (en) | Brain-derived membrane-associated CRF binding proteins | |
| Song et al. | Changes in the gene expression of a protein with the cdc10/SWI6 motif, V-1, during rat follicular development and corpus luteum formation | |
| AU721053B2 (en) | Cloning and recombinant production of CRF receptor(s) | |
| Troskie | Cloning and characterisation of gonadotropin-releasing hormone receptors from species in non-mammalian vertebrate classes: amphibia and osteichthyes | |
| Tsutsui | Gonadotropin-inhibitory hormone (GnIH) | |
| Dalkin et al. | Reproduction and fertility | |
| De Souza et al. | 36. Overview of Corticotropin-Releasing Factor Receptors and Binding Protein | |
| Vasauskas | Expression and storage of squirrel monkey chorionic gonadotropin in the pituitary gland |