ATE81510T1 - Loeslichkeits-, reinigungs- und charakterisierungsverfahren fuer proteine aus unloeslichen proteinaggregaten oder proteinkomplexen und diesbezuegliche praeparate. - Google Patents

Loeslichkeits-, reinigungs- und charakterisierungsverfahren fuer proteine aus unloeslichen proteinaggregaten oder proteinkomplexen und diesbezuegliche praeparate.

Info

Publication number
ATE81510T1
ATE81510T1 AT86101209T AT86101209T ATE81510T1 AT E81510 T1 ATE81510 T1 AT E81510T1 AT 86101209 T AT86101209 T AT 86101209T AT 86101209 T AT86101209 T AT 86101209T AT E81510 T1 ATE81510 T1 AT E81510T1
Authority
AT
Austria
Prior art keywords
proteins
purification
characterization
solubility
methods
Prior art date
Application number
AT86101209T
Other languages
English (en)
Inventor
Conrad Jean Heilman Jr
Original Assignee
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Cyanamid Co filed Critical American Cyanamid Co
Application granted granted Critical
Publication of ATE81510T1 publication Critical patent/ATE81510T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/826—Viruses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT86101209T 1985-02-27 1986-01-30 Loeslichkeits-, reinigungs- und charakterisierungsverfahren fuer proteine aus unloeslichen proteinaggregaten oder proteinkomplexen und diesbezuegliche praeparate. ATE81510T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/706,533 US4659568A (en) 1985-02-27 1985-02-27 Process for solubilization, purification and characterization of protein from insoluble protein aggregates or complexes and compositions of matter therefrom
EP86101209A EP0192988B1 (de) 1985-02-27 1986-01-30 Löslichkeits-, Reinigungs- und Charakterisierungsverfahren für Proteine aus unlöslichen Proteinaggregaten oder Proteinkomplexen und diesbezügliche Präparate

Publications (1)

Publication Number Publication Date
ATE81510T1 true ATE81510T1 (de) 1992-10-15

Family

ID=24838031

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86101209T ATE81510T1 (de) 1985-02-27 1986-01-30 Loeslichkeits-, reinigungs- und charakterisierungsverfahren fuer proteine aus unloeslichen proteinaggregaten oder proteinkomplexen und diesbezuegliche praeparate.

Country Status (6)

Country Link
US (1) US4659568A (de)
EP (1) EP0192988B1 (de)
JP (1) JPH0660197B2 (de)
AT (1) ATE81510T1 (de)
CA (1) CA1285699C (de)
DE (1) DE3686954T2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
AU597924B2 (en) * 1985-12-11 1990-06-14 Natinco Nv Solubilization of protein aggregates
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4743583A (en) * 1987-07-20 1988-05-10 Temple University Sustained release protein compositions and method for making
DE3723992A1 (de) 1987-07-20 1989-02-02 Boehringer Mannheim Gmbh Verfahren zur herstellung von proteinen in loeslicher form
US5520817A (en) * 1994-10-04 1996-05-28 Biologica Co. Method of fractionating components in liquid chromatography
WO2000002901A1 (en) 1998-07-09 2000-01-20 University Technology Corporation High pressure refolding of protein aggregates and inclusion bodies
EP1434789B1 (de) * 2000-10-31 2008-03-26 BaroFold, Inc. Verbesserter zerfall und rückfaltung von proteinen mittels hohen drucks
FR2860796B1 (fr) * 2003-10-14 2006-02-10 Centre Nat Rech Scient Procede de dissociation de la molecule d'hemoglobine extracellulaire d'arenicola marina, chaines proteiques constituant ladite molecule et sequences nucleotidiques codant pour lesdites chaines proteiques
GB0407701D0 (en) * 2004-04-05 2004-05-12 Johnson Matthey Plc Adhesion promoting compound
CA2663416A1 (en) * 2006-09-15 2008-03-20 Barofold, Inc. High pressure treatment of proteins for reduced immunogenicity
DE102008030142A1 (de) * 2008-06-27 2009-12-31 Qiagen Gmbh Verfahren zur Proteinaufreinigung unter denaturierenden Bedingungen
US9539233B2 (en) 2009-05-04 2017-01-10 Aridis Pharmaceuticals Inc. Gallium formulation for the treatment and prevention of infectious diseases
GB201204160D0 (en) * 2012-03-09 2012-04-25 Nandi Proteins Ltd Process for modifying proteins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3519400A (en) * 1967-01-25 1970-07-07 Atomic Energy Commission Method of centrifugal separation and recovery of chemical species utilizing a liquid medium
GB1356413A (en) * 1971-09-09 1974-06-12 Pfizer Ltd Method of obtaining purified australia antigen from blood serum
PT77014B (en) * 1982-07-20 1986-01-24 Molecular Genetics Inc Production of herpes simplex viral proteins
DE3228501A1 (de) * 1982-07-30 1984-02-02 Behringwerke Ag, 3550 Marburg Verfahren zur herstellung eines herpes-antigens, dazu geeignetes mittel sowie verfahren zu seiner herstellung und die verwendung dieses antigens

Also Published As

Publication number Publication date
EP0192988B1 (de) 1992-10-14
EP0192988A2 (de) 1986-09-03
JPH0660197B2 (ja) 1994-08-10
CA1285699C (en) 1991-07-02
DE3686954T2 (de) 1993-04-01
DE3686954D1 (de) 1992-11-19
JPS61267599A (ja) 1986-11-27
US4659568A (en) 1987-04-21
EP0192988A3 (en) 1989-03-15

Similar Documents

Publication Publication Date Title
ATE81510T1 (de) Loeslichkeits-, reinigungs- und charakterisierungsverfahren fuer proteine aus unloeslichen proteinaggregaten oder proteinkomplexen und diesbezuegliche praeparate.
GB2199328B (en) Use of a 5' untranslated leader sequence of an rna virus to enhance translation
ATE79138T1 (de) Typenspezifische papillomavirus-dns-sequenzen und peptide.
DK0733067T3 (da) N-terminalt kemisk modificerede proteinpræparater og fremgangsmåder
DK0662827T3 (da) Terapeutiske og diagnostiske fremgangsmåder og sammensætninger baseret på Notch-proteiner og nucleinsyrer
DE68929043D1 (de) Für androgen-rezeptor-protein kodierende dna
DK394786D0 (da) Fremgangsmaade til rensning og aktivering af proteiner fra uoploeselige inklusionslegemer
FI103506B1 (fi) Menetelmä syklisten aminohappojohdannaisten valmistamiseksi ja menetelmässä käytettävä välituote
NO852567L (no) Thymopentinanaloger og fremgangsmmaate for deres fremstilling.
DE3650135D1 (de) Proteingewinnung.
DE69133509D1 (de) Verfahren zur herstellung von beta-lactamen
PT87537A (pt) Process for the preparation of cyclic anticoagulant peptides
PT87536A (pt) Process for the preparation of cyclic anticoagulant peptides
ATE43639T1 (de) Verfahren zur entfernung von verunreinigungen aus leukozytinterferon-zubereitungen und das so hergestellte gereinigte leukozytinterferon.
PT85053A (de) Peptide mit einfluss auf die diurese und natriurese verfahren zu ihrer herstellung diese enthaltende mittel und ihre verwendung
ES8704511A1 (es) Procedimiento para sintetizar peptidos a partir de aminoaci-dos
NO930428L (no) Proteinstrukturen til plantetoksingelonin
ATE96805T1 (de) Verfahren zur rueckgewinnung rekombinanter proteine.
ATE41426T1 (de) Verfahren zur herstellung von triacetoxysilanen aus tris(amino)silanen.
ATE356831T1 (de) Neuer galaninrezeptor
DE3687995D1 (de) Verfahren zur reinigung von interferon und so hergestellte zusammensetzung.
DK0463053T3 (da) Fremstilling og anvendelse af anthelmintiske midler og beskyttende immunogener
ES370345A1 (es) Un procedimiento para fabricar una preparacion inyectable de insulina.
DK102787D0 (da) Isohexidnucleosider og farmaceutiske praeparater indeholdende disse
DE69207181D1 (de) Verfahren zur inaktivierung von viren

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee