ATE554164T1 - Primer für nukleinsäureamplifikation bei der detektion von haushaltsgen-mrna und testverfahren unter verwendung der primer - Google Patents
Primer für nukleinsäureamplifikation bei der detektion von haushaltsgen-mrna und testverfahren unter verwendung der primerInfo
- Publication number
- ATE554164T1 ATE554164T1 AT09007530T AT09007530T ATE554164T1 AT E554164 T1 ATE554164 T1 AT E554164T1 AT 09007530 T AT09007530 T AT 09007530T AT 09007530 T AT09007530 T AT 09007530T AT E554164 T1 ATE554164 T1 AT E554164T1
- Authority
- AT
- Austria
- Prior art keywords
- primers
- nucleic acid
- acid amplification
- testing methods
- gene mrna
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 3
- 108020004999 messenger RNA Proteins 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000012360 testing method Methods 0.000 title 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 abstract 2
- 108700039887 Essential Genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
- C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002043866 | 2002-02-20 | ||
| JP2002043867 | 2002-02-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE554164T1 true ATE554164T1 (de) | 2012-05-15 |
Family
ID=27759655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT09007530T ATE554164T1 (de) | 2002-02-20 | 2003-02-13 | Primer für nukleinsäureamplifikation bei der detektion von haushaltsgen-mrna und testverfahren unter verwendung der primer |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20050164190A1 (de) |
| EP (2) | EP2107113B1 (de) |
| JP (2) | JP4426309B2 (de) |
| CN (1) | CN100516211C (de) |
| AT (1) | ATE554164T1 (de) |
| AU (1) | AU2003211947A1 (de) |
| ES (1) | ES2382868T3 (de) |
| WO (1) | WO2003070935A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4977325B2 (ja) * | 2004-10-15 | 2012-07-18 | シスメックス株式会社 | 精度管理用疑似組織及びそれを用いた精度管理方法 |
| EP1893776A2 (de) * | 2005-06-03 | 2008-03-05 | Aviaradx, Inc. | Normierungsgene |
| JP2008136389A (ja) * | 2006-11-30 | 2008-06-19 | Sapporo Breweries Ltd | アップルモザイクウイルスの検出方法、検出用プライマーセット及び検出用キット |
| US8557523B2 (en) * | 2007-10-19 | 2013-10-15 | Eiken Kagaku Kabushiki Kaisha | Method of nucleic acid amplification and measuring reagent and reagent kit therefor |
| US20110053168A1 (en) * | 2008-03-28 | 2011-03-03 | Sysmex Corporation | REAGENT COMPRISING PRIMER FOR DETECTION OF mRNA FOR CYTOKERATIN-7 |
| JP5822824B2 (ja) * | 2009-06-04 | 2015-11-24 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 複合核酸の増幅 |
| JP5710190B2 (ja) * | 2010-09-16 | 2015-04-30 | 株式会社東芝 | βアクチン遺伝子のためのプライマーセット、プローブ、アッセイキットおよび検出方法 |
| JP5782704B2 (ja) * | 2010-11-11 | 2015-09-24 | 東ソー株式会社 | ＧＡＰＤＨｍＲＮＡの測定方法 |
| CN106636348A (zh) * | 2016-11-08 | 2017-05-10 | 河南师范大学 | 运动发酵单胞菌环介导恒温基因扩增快速检测试剂盒及检测方法 |
| CN107287320A (zh) * | 2017-07-12 | 2017-10-24 | 曹国君 | Gapdh基因的lamp检测用引物组合及试剂盒 |
| US11788129B2 (en) * | 2021-02-23 | 2023-10-17 | Industrial Technology Research Institute | Nucleic acid detection kit and nucleic acid detection method |
| WO2022201181A1 (en) * | 2021-03-24 | 2022-09-29 | Indian Council Of Medical Research | RT-LAMP ASSAY FOR DETECTION OF HUMAN β-ACTIN HOUSEKEEPING GENE |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0174608A1 (de) | 1984-09-13 | 1986-03-19 | The Board Of Trustees Of The Leland Stanford Junior University | Beta-Actin-Gen und regulatorische Elemente, deren Herstellung und Verwendung |
| JP2650159B2 (ja) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| WO1994017086A1 (en) * | 1993-01-25 | 1994-08-04 | Apollon, Inc. | Gene regulation by targeting putative intramolecular triple helix |
| US5639606A (en) * | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| US5876978A (en) * | 1993-04-06 | 1999-03-02 | Medical College Of Ohio | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| US5643765A (en) * | 1993-04-06 | 1997-07-01 | University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| DE4416446A1 (de) | 1993-05-12 | 1994-11-17 | Becton Dickinson Co | Für menschliche DNA spezifische Sequenzen |
| JPH07123984A (ja) * | 1993-11-05 | 1995-05-16 | Hitachi Chem Co Ltd | 特異的メッセンジャーｒｎａを検出・測定するためのプライマー |
| EP0907747A1 (de) | 1996-03-15 | 1999-04-14 | Yale University | Verfahren und anordnung zur detektion von stickstoffoxid-synthease |
| GB9607441D0 (en) * | 1996-04-10 | 1996-06-12 | Univ Manitoba | Human hyaluronan receptor |
| US5955268A (en) * | 1996-04-26 | 1999-09-21 | Abbott Laboratories | Method and reagent for detecting multiple nucleic acid sequences in a test sample |
| US6258543B1 (en) * | 1996-05-02 | 2001-07-10 | Xtrana, Inc. | Quantitation of RNA transcripts using genomic DNA as the internal amplification competitor |
| US5817912A (en) * | 1997-01-16 | 1998-10-06 | B.M.R.A. Corporation B.V. | Transgenic mice with disrupted NPY Y1 receptor genes |
| US20030096232A1 (en) * | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
| DE19804372A1 (de) * | 1998-02-04 | 1999-08-05 | Michael W Dr Dr Dahm | Verfahren zur quantitativen Bestimmung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits |
| AU3867199A (en) * | 1998-04-23 | 1999-11-08 | Genentech Inc. | Quantitative analysis of gene expression |
| US6576420B1 (en) * | 1998-06-23 | 2003-06-10 | Regents Of The University Of California | Method for early diagnosis of, and determination of prognosis in, cancer |
| US6743602B1 (en) * | 1999-07-26 | 2004-06-01 | Chiron Corporation | Polynucleotides differentially expressed in adenocarcinomas, polypeptides encoded thereby, and methods of use thereof |
| DE69940623D1 (de) * | 1998-11-09 | 2009-04-30 | Eiken Chemical | Prozess zur Synthetisierung von Nukleinsäure |
| US7135284B1 (en) * | 1999-02-05 | 2006-11-14 | Integrated Dna Technologies, Inc. | Primer extension methods for production of high specific activity nucleic acid probes |
| DE19909503A1 (de) | 1999-03-04 | 2000-09-07 | Boehringer Ingelheim Int | Tumorassoziiertes Antigen |
| US7607022B1 (en) * | 1999-06-11 | 2009-10-20 | General Instrument Corporation | Configurable encryption/decryption for multiple services support |
| US20060009393A1 (en) * | 1999-10-02 | 2006-01-12 | The Government of the U.S.A as represented by the Secretary of the Dept. of Health & Human Services | Immunogenic epitopes for fibroblast growth factors 5 (FGF-5) |
| JP2003523207A (ja) | 2000-01-25 | 2003-08-05 | ジェネンテック・インコーポレーテッド | Ｌｉｖ−１関連タンパク質、それをコードするポリヌクレオチド、及び癌の治療へのその利用 |
| US6618679B2 (en) * | 2000-01-28 | 2003-09-09 | Althea Technologies, Inc. | Methods for analysis of gene expression |
| JP4437856B2 (ja) | 2000-02-29 | 2010-03-24 | 栄研化学株式会社 | 同一鎖内に相補性領域を有する核酸の検出方法 |
| IL137865A0 (en) | 2000-08-15 | 2001-10-31 | Yeda Res & Dev | Method for the diagnosis and follow-up of schizophrenia |
| ATE437225T1 (de) | 2000-09-19 | 2009-08-15 | Eiken Chemical | Verfahren zur polynukleotidsynthese |
| KR100762261B1 (ko) * | 2001-04-27 | 2007-10-04 | (주)바이오니아 | 전장 상보 디옥시리보핵산 제조 방법과 이에 사용되는앵커와 프라이머 |
| US20050118591A1 (en) * | 2001-11-22 | 2005-06-02 | Adnagen Ag | Diagnosis kit, dna chip, and methods for diagnosing or supervising the treatment of testicular cancer |
| EP1461350A4 (de) * | 2001-11-30 | 2006-07-26 | Univ Rochester | Quantitative multiplex-pcr in echtzeit |
-
2003
- 2003-02-13 CN CNB038040697A patent/CN100516211C/zh not_active Expired - Fee Related
- 2003-02-13 US US10/504,930 patent/US20050164190A1/en not_active Abandoned
- 2003-02-13 JP JP2003569828A patent/JP4426309B2/ja not_active Expired - Lifetime
- 2003-02-13 EP EP09007530A patent/EP2107113B1/de not_active Expired - Lifetime
- 2003-02-13 AU AU2003211947A patent/AU2003211947A1/en not_active Abandoned
- 2003-02-13 ES ES09007530T patent/ES2382868T3/es not_active Expired - Lifetime
- 2003-02-13 WO PCT/JP2003/001474 patent/WO2003070935A1/ja not_active Ceased
- 2003-02-13 AT AT09007530T patent/ATE554164T1/de active
- 2003-02-13 EP EP03705099A patent/EP1484394A4/de not_active Withdrawn
-
2009
- 2009-10-19 JP JP2009240455A patent/JP4426640B1/ja not_active Expired - Fee Related
-
2011
- 2011-07-25 US US13/190,317 patent/US8198052B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPWO2003070935A1 (ja) | 2005-06-09 |
| EP1484394A4 (de) | 2005-11-09 |
| WO2003070935A1 (en) | 2003-08-28 |
| EP1484394A1 (de) | 2004-12-08 |
| CN1633500A (zh) | 2005-06-29 |
| AU2003211947A1 (en) | 2003-09-09 |
| ES2382868T3 (es) | 2012-06-14 |
| CN100516211C (zh) | 2009-07-22 |
| EP2107113A1 (de) | 2009-10-07 |
| JP4426640B1 (ja) | 2010-03-03 |
| JP4426309B2 (ja) | 2010-03-03 |
| EP2107113B1 (de) | 2012-04-18 |
| US8198052B2 (en) | 2012-06-12 |
| US20050164190A1 (en) | 2005-07-28 |
| JP2010046078A (ja) | 2010-03-04 |
| US20110281275A1 (en) | 2011-11-17 |
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