ATE528393T1 - Verfahren zur reinigung von protein und glucosedehydrogenase - Google Patents
Verfahren zur reinigung von protein und glucosedehydrogenaseInfo
- Publication number
- ATE528393T1 ATE528393T1 AT03794088T AT03794088T ATE528393T1 AT E528393 T1 ATE528393 T1 AT E528393T1 AT 03794088 T AT03794088 T AT 03794088T AT 03794088 T AT03794088 T AT 03794088T AT E528393 T1 ATE528393 T1 AT E528393T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- target protein
- glucose dehydrogenase
- liquid chromatography
- packing agent
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 8
- 102000004169 proteins and genes Human genes 0.000 title abstract 8
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- 238000004811 liquid chromatography Methods 0.000 abstract 3
- 238000012856 packing Methods 0.000 abstract 3
- 239000012460 protein solution Substances 0.000 abstract 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 238000005571 anion exchange chromatography Methods 0.000 abstract 1
- 229940099352 cholate Drugs 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000006356 dehydrogenation reaction Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000003480 eluent Substances 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 230000027756 respiratory electron transport chain Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002253742 | 2002-08-30 | ||
| PCT/JP2003/010540 WO2004022732A1 (ja) | 2002-08-30 | 2003-08-20 | タンパク質およびグルコース脱水素酵素の精製方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE528393T1 true ATE528393T1 (de) | 2011-10-15 |
Family
ID=31972809
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03794088T ATE528393T1 (de) | 2002-08-30 | 2003-08-20 | Verfahren zur reinigung von protein und glucosedehydrogenase |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20060094098A1 (de) |
| EP (1) | EP1535997B1 (de) |
| JP (1) | JP4537850B2 (de) |
| CN (1) | CN100587066C (de) |
| AT (1) | ATE528393T1 (de) |
| AU (1) | AU2003262258A1 (de) |
| WO (1) | WO2004022732A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE505724T1 (de) | 2001-09-14 | 2011-04-15 | Arkray Inc | Verfahren, gerät und vorrichtung zur konzentrationsmessung |
| EP1661516B1 (de) * | 2003-09-02 | 2012-08-15 | Koji Sode | Glukosesensor und glukosespiegel-messgerät |
| DE602005025104D1 (de) | 2004-04-23 | 2011-01-13 | Arkray Inc | Mutierte glucosedehydrogenase |
| JP5598810B2 (ja) * | 2009-04-10 | 2014-10-01 | 国立大学法人三重大学 | コイ由来抗菌剤の製造方法及び抗菌処理装置 |
| TWI400128B (zh) * | 2009-11-30 | 2013-07-01 | Univ Tamkang | 蛋白質純化方法 |
| SG10201508401TA (en) * | 2010-10-11 | 2015-11-27 | Abbvie Bahamas Ltd | Processes for purification of proteins |
| AU2011343813B2 (en) | 2010-12-15 | 2015-05-21 | Takeda Pharmaceutical Company Limited | Eluate collection using conductivity gradient |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5768138A (en) * | 1980-10-11 | 1982-04-26 | Oriental Yeast Co Ltd | Chromatography adsorbent and refining method of enzyme using this |
| US5250415A (en) * | 1987-04-08 | 1993-10-05 | Merck Patent Gesellschaft Mit Beschrankter Haftung | Process for the preparation of glucose dehydrogenase from Bacillus megaterium |
| DE3711881A1 (de) | 1987-04-08 | 1988-10-27 | Merck Patent Gmbh | Verfahren zur herstellung von glucosedehydrogenase aus bacillus megaterium |
| US5939536A (en) * | 1990-03-05 | 1999-08-17 | Genzyme Corporation | Methods for purifying cystic fibrosis transmembrane conductance regulation |
| AU2002210991B2 (en) | 2000-10-31 | 2006-08-10 | Koji Sode | Novel glucose dehydrogenase and process for producing the dehydrogenase |
-
2003
- 2003-08-20 WO PCT/JP2003/010540 patent/WO2004022732A1/ja not_active Ceased
- 2003-08-20 JP JP2004534105A patent/JP4537850B2/ja not_active Expired - Fee Related
- 2003-08-20 EP EP03794088A patent/EP1535997B1/de not_active Expired - Lifetime
- 2003-08-20 AU AU2003262258A patent/AU2003262258A1/en not_active Abandoned
- 2003-08-20 CN CN03824734A patent/CN100587066C/zh not_active Expired - Lifetime
- 2003-08-20 AT AT03794088T patent/ATE528393T1/de not_active IP Right Cessation
- 2003-08-20 US US10/526,026 patent/US20060094098A1/en not_active Abandoned
-
2011
- 2011-12-01 US US13/308,994 patent/US9206398B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1535997A1 (de) | 2005-06-01 |
| AU2003262258A1 (en) | 2004-03-29 |
| US20120309072A1 (en) | 2012-12-06 |
| CN100587066C (zh) | 2010-02-03 |
| CN1751119A (zh) | 2006-03-22 |
| JPWO2004022732A1 (ja) | 2005-12-22 |
| JP4537850B2 (ja) | 2010-09-08 |
| AU2003262258A8 (en) | 2004-03-29 |
| US20060094098A1 (en) | 2006-05-04 |
| EP1535997B1 (de) | 2011-10-12 |
| US9206398B2 (en) | 2015-12-08 |
| EP1535997A4 (de) | 2007-07-11 |
| WO2004022732A1 (ja) | 2004-03-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Holstein et al. | Improving selectivity in multimodal chromatography using controlled pH gradient elution | |
| ATE337058T1 (de) | Kolonne und mit dieser durchgeführtes chromatographisches verfahren | |
| SE8200715L (sv) | Reduktion av pyrogenhalt | |
| WO2005016968A3 (en) | Reducing protein a leaching during protein a affinity chromatography | |
| WO2005100394A3 (en) | PURIFIED INTERLEUKIN-15/Fc FUSION PROTEIN AND PREPARATION THEREOF | |
| HU212362B (en) | Methods for preparing coagulation factor ix and pharmaceutical compositions containing same | |
| DE502005007091D1 (de) | Verfahren zur reinigung von erythropoietin | |
| AU2003262258A8 (en) | Method of purifying protein and glucose dehydrogenase | |
| MX2009005012A (es) | Una forma pura de rapamicina y procedimiento para la recuperacion y purificacion de la misma. | |
| IL182340A (en) | A method of isolating hepatoglobin from the V segment of human COHN | |
| FR2686883B1 (fr) | Procede de fabrication de fibrinogene de tres haute purete, fibrinogene de tres haute purete obtenu ainsi qu'une composition pharmaceutique le contenant. | |
| JPS5517364A (en) | Purification of pancreatic kallikrein | |
| ATE522617T1 (de) | Verfahren zur aufreinigung von interferon-beta | |
| WO2004008942A3 (en) | Rapid process for purification and concentration of plasmin | |
| JPS60992B2 (ja) | コホルマイシンおよび関連物質の分離法 | |
| Fujita et al. | Heterologous production of desferrioxamines with a fusion biosynthetic gene cluster | |
| Parniak et al. | On the phosphate content of rat liver phenylalanine hydroxylase purified by hydrophobic chromatography | |
| SE0203551D0 (sv) | Prothrombin purification | |
| DK118789A (da) | Fremgangsmaade til affinitetschromatografisk rensning af a-underenheden af faktor xiii | |
| ATE510913T1 (de) | Verfahren zur isolierung und trennung von einzel- und doppelsträngigen nucleinsäuren sowie trennsäule zur durchführung des verfahrens | |
| WO2004071405A3 (en) | Lactate dehydrogenase: a target and reagent for diabetes therapy | |
| JPS5777960A (en) | Affinitive adsorbent for affnity chromatography | |
| JPS61234792A (ja) | マウスインタ−フエロンの精製法 | |
| CA2746502A1 (en) | Purification of recombinantly produced interferon | |
| KR830001822B1 (ko) | 유로 키나제의 제조방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |