ATE528393T1 - Verfahren zur reinigung von protein und glucosedehydrogenase - Google Patents

Verfahren zur reinigung von protein und glucosedehydrogenase

Info

Publication number
ATE528393T1
ATE528393T1 AT03794088T AT03794088T ATE528393T1 AT E528393 T1 ATE528393 T1 AT E528393T1 AT 03794088 T AT03794088 T AT 03794088T AT 03794088 T AT03794088 T AT 03794088T AT E528393 T1 ATE528393 T1 AT E528393T1
Authority
AT
Austria
Prior art keywords
protein
target protein
glucose dehydrogenase
liquid chromatography
packing agent
Prior art date
Application number
AT03794088T
Other languages
English (en)
Inventor
Hideaki Yamaoka
Keisuke Kurosaka
Shido Kawase
Original Assignee
Arkray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray Inc filed Critical Arkray Inc
Application granted granted Critical
Publication of ATE528393T1 publication Critical patent/ATE528393T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT03794088T 2002-08-30 2003-08-20 Verfahren zur reinigung von protein und glucosedehydrogenase ATE528393T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002253742 2002-08-30
PCT/JP2003/010540 WO2004022732A1 (ja) 2002-08-30 2003-08-20 タンパク質およびグルコース脱水素酵素の精製方法

Publications (1)

Publication Number Publication Date
ATE528393T1 true ATE528393T1 (de) 2011-10-15

Family

ID=31972809

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03794088T ATE528393T1 (de) 2002-08-30 2003-08-20 Verfahren zur reinigung von protein und glucosedehydrogenase

Country Status (7)

Country Link
US (2) US20060094098A1 (de)
EP (1) EP1535997B1 (de)
JP (1) JP4537850B2 (de)
CN (1) CN100587066C (de)
AT (1) ATE528393T1 (de)
AU (1) AU2003262258A1 (de)
WO (1) WO2004022732A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2330407E (pt) 2001-09-14 2013-07-09 Arkray Inc Método, ferramenta e dispositivo para medir a concentração
CN100479750C (zh) * 2003-09-02 2009-04-22 早出广司 葡萄糖传感器和葡萄糖浓度测定装置
KR101186718B1 (ko) * 2004-04-23 2012-09-27 아크레이 가부시키가이샤 변이 글루코오스 탈수소효소
JP5598810B2 (ja) * 2009-04-10 2014-10-01 国立大学法人三重大学 コイ由来抗菌剤の製造方法及び抗菌処理装置
TWI400128B (zh) * 2009-11-30 2013-07-01 Univ Tamkang 蛋白質純化方法
WO2012051147A1 (en) * 2010-10-11 2012-04-19 Abbott Laboratories Processes for purification of proteins
CA2821711C (en) 2010-12-15 2017-10-10 Baxter International Inc. Eluate collection using conductivity gradient

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5768138A (en) * 1980-10-11 1982-04-26 Oriental Yeast Co Ltd Chromatography adsorbent and refining method of enzyme using this
US5250415A (en) * 1987-04-08 1993-10-05 Merck Patent Gesellschaft Mit Beschrankter Haftung Process for the preparation of glucose dehydrogenase from Bacillus megaterium
DE3711881A1 (de) * 1987-04-08 1988-10-27 Merck Patent Gmbh Verfahren zur herstellung von glucosedehydrogenase aus bacillus megaterium
US5939536A (en) * 1990-03-05 1999-08-17 Genzyme Corporation Methods for purifying cystic fibrosis transmembrane conductance regulation
AU2002210991B2 (en) 2000-10-31 2006-08-10 Koji Sode Novel glucose dehydrogenase and process for producing the dehydrogenase

Also Published As

Publication number Publication date
EP1535997A4 (de) 2007-07-11
JPWO2004022732A1 (ja) 2005-12-22
AU2003262258A1 (en) 2004-03-29
JP4537850B2 (ja) 2010-09-08
WO2004022732A1 (ja) 2004-03-18
AU2003262258A8 (en) 2004-03-29
CN100587066C (zh) 2010-02-03
EP1535997A1 (de) 2005-06-01
EP1535997B1 (de) 2011-10-12
US9206398B2 (en) 2015-12-08
US20120309072A1 (en) 2012-12-06
CN1751119A (zh) 2006-03-22
US20060094098A1 (en) 2006-05-04

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Legal Events

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