ATE525469T1 - Nachweis kleiner nukleinsäuren - Google Patents

Nachweis kleiner nukleinsäuren

Info

Publication number
ATE525469T1
ATE525469T1 AT03813850T AT03813850T ATE525469T1 AT E525469 T1 ATE525469 T1 AT E525469T1 AT 03813850 T AT03813850 T AT 03813850T AT 03813850 T AT03813850 T AT 03813850T AT E525469 T1 ATE525469 T1 AT E525469T1
Authority
AT
Austria
Prior art keywords
detection
present
nucleic acids
methods
mirnas
Prior art date
Application number
AT03813850T
Other languages
English (en)
Inventor
James Dahlberg
Hatim Allawi
Victor Lyamichev
Bruce Neri
Marilyn Olson-Munoz
Luanne Chehak
Sarah Olson
Original Assignee
Third Wave Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Third Wave Tech Inc filed Critical Third Wave Tech Inc
Priority claimed from US10/740,256 external-priority patent/US7851150B2/en
Application granted granted Critical
Publication of ATE525469T1 publication Critical patent/ATE525469T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
AT03813850T 2002-12-18 2003-12-18 Nachweis kleiner nukleinsäuren ATE525469T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US43451802P 2002-12-18 2002-12-18
PCT/US2003/041549 WO2004057017A2 (en) 2002-12-18 2003-12-18 Detection of small nucleic acids
US10/740,256 US7851150B2 (en) 2002-12-18 2003-12-18 Detection of small nucleic acids

Publications (1)

Publication Number Publication Date
ATE525469T1 true ATE525469T1 (de) 2011-10-15

Family

ID=32685318

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03813850T ATE525469T1 (de) 2002-12-18 2003-12-18 Nachweis kleiner nukleinsäuren

Country Status (9)

Country Link
EP (2) EP2361991A1 (de)
JP (1) JP4230457B2 (de)
AT (1) ATE525469T1 (de)
AU (1) AU2003297541A1 (de)
CA (1) CA2510381C (de)
HK (1) HK1083111A1 (de)
MX (1) MXPA05006633A (de)
NO (1) NO20053465L (de)
WO (1) WO2004057017A2 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
AU2003291433B2 (en) 2002-11-13 2008-05-22 Thomas Jefferson University Compositions and methods for cancer diagnosis and therapy
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
US7683036B2 (en) 2003-07-31 2010-03-23 Regulus Therapeutics Inc. Oligomeric compounds and compositions for use in modulation of small non-coding RNAs
CA2539654A1 (en) * 2003-10-14 2005-05-06 Novartis Ag Oligonucleotide microarray
JP5192229B2 (ja) * 2004-04-07 2013-05-08 エクシコン・アクティーゼルスカブ microRNAおよび低分子干渉RNAの定量化のための新規方法
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
WO2006033020A2 (en) * 2004-04-26 2006-03-30 Rosetta Genomics Ltd. Methods and apparatus for the detection and validation of micrornas
EP1784501B1 (de) 2004-05-14 2015-11-18 Rosetta Genomics Ltd Micro-rnas und deren verwendung
US7795419B2 (en) 2004-05-26 2010-09-14 Rosetta Genomics Ltd. Viral and viral associated miRNAs and uses thereof
AU2005250432B2 (en) 2004-05-28 2011-09-15 Asuragen, Inc. Methods and compositions involving microRNA
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules
ES2534304T3 (es) 2004-11-12 2015-04-21 Asuragen, Inc. Procedimientos y composiciones que implican miARN y moléculas inhibidoras de miARN
WO2006078042A1 (ja) * 2005-01-20 2006-07-27 Kurume University 小分子rnaの検出方法および小分子rna検出用試薬
JP2006292367A (ja) * 2005-04-05 2006-10-26 Mitsubishi Rayon Co Ltd miRNA検出用マイクロアレイ
WO2006135765A1 (en) * 2005-06-09 2006-12-21 Epoch Biosciences, Inc. Improved primer-based amplification methods
ATE491045T1 (de) 2005-06-23 2010-12-15 Keygene Nv Strategien mit hohem durchsatz zur identifizierung und zum nachweis von polymorphismen
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
JP5237099B2 (ja) 2005-09-29 2013-07-17 キージーン ナムローゼ フェンノートシャップ 変異させた集団のハイスループットスクリーニング
WO2007073165A1 (en) 2005-12-22 2007-06-28 Keygene N.V. Method for high-throughput aflp-based polymorphism detection
JP4989493B2 (ja) * 2006-01-20 2012-08-01 オリンパス株式会社 分子内プローブによる核酸配列の検出方法
ES2545264T3 (es) 2006-04-04 2015-09-09 Keygene N.V. Detección de alto rendimiento de marcadores moleculares basada en fragmentos de restricción
CN101541975B (zh) * 2006-06-01 2013-04-03 第三次浪潮技术公司 核酸的检测
US8445238B2 (en) 2006-10-04 2013-05-21 Third Wave Technologies, Inc. Snap-back primers and detectable hairpin structures
US8361714B2 (en) 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
EP2285960B1 (de) 2008-05-08 2015-07-08 Asuragen, INC. Zusammensetzungen und verfahren in zusammenhang mit der mirna-184modulation von neovaskularisation oder angiogenese
JP5322066B2 (ja) 2008-07-03 2013-10-23 国立大学法人神戸大学 関節リウマチ関連マイクロrna
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
GB2520763A (en) 2013-12-02 2015-06-03 Vanadis Diagnostics Ab Nucleic acid probe and method of detecting genomic fragments
JP6688318B2 (ja) * 2015-05-01 2020-04-28 ジェン−プローブ・インコーポレーテッド 多重侵入切断アッセイ
BR112018001686A2 (pt) 2015-09-18 2018-09-18 Vanadis Diagnostics conjunto de sonda para analisar uma amostra de dna e método para usar o mesmo
EP3668998A1 (de) 2017-10-06 2020-06-24 Cartana AB Rna-template-ligation
JP2024000312A (ja) * 2022-06-20 2024-01-05 Toppanホールディングス株式会社 蛍光物質及び消光物質で標識された一本鎖オリゴヌクレオチド
WO2023248568A1 (ja) * 2022-06-20 2023-12-28 Toppanホールディングス株式会社 蛍光を検出するための方法

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5288609A (en) 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5359100A (en) 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5925525A (en) 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US5849481A (en) 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US7150982B2 (en) 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5614402A (en) 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
US6096880A (en) * 1993-04-15 2000-08-01 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US6068818A (en) 1993-11-01 2000-05-30 Nanogen, Inc. Multicomponent devices for molecular biological analysis and diagnostics
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AU8102694A (en) 1993-11-17 1995-06-06 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
CA2182517C (en) 1994-02-07 2001-08-21 Theo Nikiforov Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis
US5851770A (en) 1994-04-25 1998-12-22 Variagenics, Inc. Detection of mismatches by resolvase cleavage using a magnetic bead support
AU693198B2 (en) 1994-04-25 1998-06-25 Avitech Diagnostics, Inc. Detection of mutation by resolvase cleavage
EP1260593A3 (de) 1994-12-23 2003-12-03 Dade Behring Inc. Nachweis von Nukleinsäuren durch Nuklease-katalysierte Produktbildung
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
AU714486B2 (en) 1995-11-21 2000-01-06 Yale University Unimolecular segment amplification and detection
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
EP0876510B1 (de) 1995-12-22 2008-05-07 Dade Behring Marburg GmbH Homogene vervielfältigung und nachweis von nukleinsäuren
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
CA2243353C (en) 1996-01-24 2010-03-30 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
DE69738687D1 (de) 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
CA2273204C (en) 1996-11-29 2008-10-14 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods
US6001311A (en) 1997-02-05 1999-12-14 Protogene Laboratories, Inc. Apparatus for diverse chemical synthesis using two-dimensional array
AU744369B2 (en) 1997-05-05 2002-02-21 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US6194149B1 (en) 1998-03-03 2001-02-27 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US6013459A (en) 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US6063573A (en) 1998-01-27 2000-05-16 Clinical Micro Sensors, Inc. Cycling probe technology using electron transfer detection
DE69902996T2 (de) 1998-02-04 2003-04-30 Variagenics Inc Techniken zu detektion von fehlpaarungen
EP2360271A1 (de) 1998-06-24 2011-08-24 Illumina, Inc. Dekodierung von Arraysensoren mit Mikrosphären
US6316229B1 (en) 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US6221603B1 (en) * 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
JP2004521606A (ja) 2000-05-24 2004-07-22 サード・ウェーブ・テクノロジーズ・インク Rnaの検出法
WO2001098537A2 (en) 2000-06-17 2001-12-27 Third Wave Technologies, Inc. Nucleic acid accessible hybridization sites
WO2002070755A2 (en) 2000-11-15 2002-09-12 Third Wave Technologies, Inc. Fen endonucleases
WO2002099386A2 (en) * 2001-06-07 2002-12-12 Proligo Llc Microcalorimetric detection of analytes and binding events
US20030148519A1 (en) 2001-11-14 2003-08-07 Engelke David R. Intracellular expression and delivery of siRNAs in mammalian cells
GB0212995D0 (en) 2002-06-06 2002-07-17 Unilever Plc Ligand and complex for catalytically bleaching a substrate
JPWO2010084839A1 (ja) 2009-01-26 2012-07-19 日本電気株式会社 尤度推定装置、コンテンツ配信システム、尤度推定方法および尤度推定プログラム

Also Published As

Publication number Publication date
CA2510381A1 (en) 2004-07-08
NO20053465L (no) 2005-09-13
WO2004057017A2 (en) 2004-07-08
CA2510381C (en) 2014-07-08
JP4230457B2 (ja) 2009-02-25
HK1083111A1 (en) 2006-06-23
NO20053465D0 (no) 2005-07-15
EP1573009A2 (de) 2005-09-14
JP2006510372A (ja) 2006-03-30
MXPA05006633A (es) 2006-05-25
EP2361991A1 (de) 2011-08-31
EP1573009B1 (de) 2011-09-21
WO2004057017A3 (en) 2005-03-10
EP1573009A4 (de) 2006-07-12
AU2003297541A1 (en) 2004-07-14

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