WO2005040379A3 - RNA INTERFERENCE MEDIATED INHIBITION OF RAS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) - Google Patents

RNA INTERFERENCE MEDIATED INHIBITION OF RAS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) Download PDF

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Publication number
WO2005040379A3
WO2005040379A3 PCT/US2004/027333 US2004027333W WO2005040379A3 WO 2005040379 A3 WO2005040379 A3 WO 2005040379A3 US 2004027333 W US2004027333 W US 2004027333W WO 2005040379 A3 WO2005040379 A3 WO 2005040379A3
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WIPO (PCT)
Prior art keywords
ras
nucleic acid
short interfering
sina
gene expression
Prior art date
Application number
PCT/US2004/027333
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French (fr)
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WO2005040379A2 (en
Inventor
James Mcswiggen
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Sirna Therapeutics Inc
James Mcswiggen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/693,059 external-priority patent/US20080039414A1/en
Priority claimed from US10/720,448 external-priority patent/US8273866B2/en
Priority claimed from US10/727,780 external-priority patent/US20050233329A1/en
Priority claimed from US10/757,803 external-priority patent/US20050020525A1/en
Priority claimed from US10/780,447 external-priority patent/US7491805B2/en
Priority claimed from US10/826,966 external-priority patent/US20050032733A1/en
Priority claimed from PCT/US2004/013456 external-priority patent/WO2005041859A2/en
Priority claimed from PCT/US2004/016390 external-priority patent/WO2005019453A2/en
Application filed by Sirna Therapeutics Inc, James Mcswiggen filed Critical Sirna Therapeutics Inc
Priority to EP04816819A priority Critical patent/EP1675953A2/en
Publication of WO2005040379A2 publication Critical patent/WO2005040379A2/en
Publication of WO2005040379A3 publication Critical patent/WO2005040379A3/en

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Abstract

This invention relates to compounds, compositions, and methods useful for modulating RAS, e.g. K-RAS, H-RAS, and/or N-RAS gene expression using short interfering nucleic acid (siNA) molecules. This invention also relates to compounds, compositions, and methods useful for modulating the expression and activity of other genes involved in pathways of RAS, e.g. K-RAS, H-RAS, and/or N-RAS gene expression and/or activity by RNA interference (RNAi) using small nucleic acid molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules and methods used to modulate the expression of RAS genes, such as K-RAS, H-RAS, and/or N-RAS.
PCT/US2004/027333 2003-10-23 2004-08-20 RNA INTERFERENCE MEDIATED INHIBITION OF RAS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) WO2005040379A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04816819A EP1675953A2 (en) 2003-10-23 2004-08-20 RNA INTERFERENCE MEDIATED INHIBITION OF RAS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)

Applications Claiming Priority (18)

Application Number Priority Date Filing Date Title
US10/693,059 2003-10-23
US10/693,059 US20080039414A1 (en) 2002-02-20 2003-10-23 RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US10/720,448 US8273866B2 (en) 2002-02-20 2003-11-24 RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SINA)
US10/720,448 2003-11-24
US10/727,780 US20050233329A1 (en) 2002-02-20 2003-12-03 Inhibition of gene expression using duplex forming oligonucleotides
US10/727,780 2003-12-03
US10/757,803 2004-01-14
US10/757,803 US20050020525A1 (en) 2002-02-20 2004-01-14 RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US54348004P 2004-02-10 2004-02-10
US60/543,480 2004-02-10
US10/780,447 US7491805B2 (en) 2001-05-18 2004-02-13 Conjugates and compositions for cellular delivery
US10/780,447 2004-02-13
US10/826,966 2004-04-16
US10/826,966 US20050032733A1 (en) 2001-05-18 2004-04-16 RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SiNA)
USPCT/US04/013456 2004-04-30
PCT/US2004/013456 WO2005041859A2 (en) 2003-04-30 2004-04-30 Conjugates and compositions for cellular delivery.
PCT/US2004/016390 WO2005019453A2 (en) 2001-05-18 2004-05-24 RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
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Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
WO2005066363A2 (en) * 2004-01-09 2005-07-21 Medigen Biotechnology Corporation Cancer specific gene ccndbp1
EP1704228B1 (en) 2004-01-16 2012-04-11 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Immunokinases
WO2006002538A1 (en) 2004-07-02 2006-01-12 Protiva Biotherapeutics, Inc. Immunostimulatory sirna molecules and uses therefor
WO2006043014A1 (en) * 2004-10-22 2006-04-27 Neuregenix Limited Neuron regeneration
EP1915462A2 (en) * 2005-07-28 2008-04-30 Oncotherapy Science, Inc. Cancer related gene rasgef1a
CN101268194A (en) 2005-09-20 2008-09-17 巴斯福植物科学有限公司 Methods for controlling gene expression using ta-siRNA
US20070099858A1 (en) * 2005-10-03 2007-05-03 Sirna Therapeutics, Inc. RNA interference mediated of inhibition of influenza virus gene expression using short interfering nucleic acid (siNA)
AU2013203219B2 (en) * 2005-11-02 2016-09-22 Arbutus Biopharma Corporation MODIFIED siRNA MOLECULES AND USES THEREOF
EP2395012B8 (en) 2005-11-02 2018-06-06 Arbutus Biopharma Corporation Modified siRNA molecules and uses thereof
US20070218122A1 (en) * 2005-11-18 2007-09-20 Protiva Biotherapeutics, Inc. siRNA silencing of influenza virus gene expression
EP1800695A1 (en) 2005-12-21 2007-06-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Immuno-RNA-constructs
BRPI0707276B1 (en) 2006-01-27 2021-08-31 Biogen Ma Inc NOGO RECEPTOR ANTAGONIST FUSION POLYPEPTIDE
NZ587616A (en) * 2006-05-11 2012-03-30 Alnylam Pharmaceuticals Inc Compositions and methods for inhibiting expression of the pcsk9 gene
US7723052B2 (en) 2006-05-11 2010-05-25 Quark Pharmaceuticals, Inc. Screening systems utilizing RTP801
CN101489566B (en) * 2006-05-19 2012-04-18 阿尔尼拉姆医药品有限公司 Rnai modulation of aha and therapeutic uses thereof
CA2653052A1 (en) * 2006-05-19 2007-11-29 The Scripps Research Institute Treatment of protein misfolding
CA2653451C (en) * 2006-05-22 2015-12-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of ikk-b gene
EP2026843A4 (en) 2006-06-09 2011-06-22 Quark Pharmaceuticals Inc Therapeutic uses of inhibitors of rtp801l
US7915399B2 (en) 2006-06-09 2011-03-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
DE102006032424A1 (en) * 2006-07-13 2008-01-17 Ernst-Moritz-Arndt Universität Greifswald Treatment of T-cell malignancies
JP2010519912A (en) * 2007-03-02 2010-06-10 エムディーアールエヌエー，インコーポレイテッド Nucleic acid compound for suppressing expression of RAS gene and use thereof
US11078262B2 (en) 2007-04-30 2021-08-03 Allergan, Inc. High viscosity macromolecular compositions for treating ocular conditions
FR2921935B1 (en) * 2007-10-05 2011-10-28 Centre Nat Rech Scient METHOD OF SCREENING COMPOUNDS FOR TREATING RESPIRATORY DISORDERS
US8614311B2 (en) 2007-12-12 2013-12-24 Quark Pharmaceuticals, Inc. RTP801L siRNA compounds and methods of use thereof
EP2123773A1 (en) * 2008-05-21 2009-11-25 sanofi-aventis Use of GPR151 modulators for the treatment of pain
EP2756845B1 (en) * 2009-04-03 2017-03-15 Dicerna Pharmaceuticals, Inc. Methods and compositions for the specific inhibition of KRAS by asymmetric double-stranded RNA
PL3199165T3 (en) * 2009-04-03 2022-09-05 Dicerna Pharmaceuticals, Inc. Methods and compositions for the specific inhibition of kras by asymmetric double-stranded rna
EP2432499A2 (en) 2009-05-20 2012-03-28 Schering Corporation Modulation of pilr receptors to treat microbial infections
ES2655079T3 (en) 2009-09-10 2018-02-16 Merck Sharp & Dohme Corp. Use of IL-33 antagonists to treat fibrotic diseases
US8691227B2 (en) 2009-12-17 2014-04-08 Merck Sharp & Dohme Corp. Methods of treating multiple sclerosis, rheumatoid arthritis and inflammatory bowel disease using agonists antibodies to PILR-α
WO2011127175A1 (en) * 2010-04-06 2011-10-13 Isis Pharmaceuticals, Inc. Modulation of cd130 (gp130) expression
US9771579B2 (en) * 2010-06-23 2017-09-26 Curna, Inc. Treatment of sodium channel, voltage-gated, alpha subunit (SCNA) related diseases by inhibition of natural antisense transcript to SCNA
CN103140582A (en) 2010-08-24 2013-06-05 默沙东公司 Single-stranded rnai agents containing an internal, non-nucleic acid spacer
WO2012058210A1 (en) 2010-10-29 2012-05-03 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACIDS (siNA)
US9920317B2 (en) 2010-11-12 2018-03-20 The General Hospital Corporation Polycomb-associated non-coding RNAs
EP3702460A1 (en) 2010-11-12 2020-09-02 The General Hospital Corporation Polycomb-associated non-coding rnas
WO2013070771A1 (en) * 2011-11-07 2013-05-16 Isis Pharmaceuticals, Inc. Administration of factor xi antisense oligonucleotides
CN109481455A (en) * 2012-05-02 2019-03-19 箭头研究公司 The organic composite for treating KRAS related disease
JP6283661B2 (en) * 2012-05-09 2018-02-21 ストライク バイオ インク．Ｓｔｒｉｋｅ Ｂｉｏ，Ｉｎｃ． Bifunctional small hairpin RNA specific for single-base KRAS mutation (BI-SHRNA, Bi-functional Short-Hairpin RNA)
CA2873794A1 (en) 2012-05-16 2013-11-21 Rana Therapeutics Inc. Compositions and methods for modulating smn gene family expression
CN104583399A (en) 2012-05-16 2015-04-29 Rana医疗有限公司 Compositions and methods for modulating hemoglobin gene family expression
JP2015523853A (en) 2012-05-16 2015-08-20 ラナ セラピューティクス インコーポレイテッド Compositions and methods for modulating ATP2A2 expression
BR112014028634A2 (en) 2012-05-16 2017-06-27 Rana Therapeutics Inc compositions and methods for modulating utrn expression
EA201492116A1 (en) 2012-05-16 2015-05-29 Рана Терапьютикс, Инк. COMPOSITIONS AND METHODS FOR MODULATING THE EXPRESSION OF MECP2
US10837014B2 (en) 2012-05-16 2020-11-17 Translate Bio Ma, Inc. Compositions and methods for modulating SMN gene family expression
US10125369B2 (en) 2012-12-05 2018-11-13 Alnylam Pharmaceuticals, Inc. PCSK9 iRNA compositions and methods of use thereof
EP3212824A4 (en) 2014-10-30 2018-08-08 The General Hospital Corporation Methods for modulating atrx-dependent gene repression
EP3271460A4 (en) 2015-03-17 2019-03-13 The General Hospital Corporation The rna interactome of polycomb repressive complex 1 (prc1)
WO2017021855A2 (en) * 2015-07-31 2017-02-09 Vascular Biogenics Ltd. Motile sperm domain containing protein 2 and inflammation
CN108348541A (en) 2015-08-25 2018-07-31 阿尔尼拉姆医药品有限公司 Method and composition for treating proprotein convertase subtilisin KEXIN (PCSK9) gene-correlation obstacle
US11260073B2 (en) 2015-11-02 2022-03-01 Ionis Pharmaceuticals, Inc. Compounds and methods for modulating C90RF72
CN106755293B (en) * 2016-09-23 2020-09-04 宁波大学 Lung cancer auxiliary diagnosis related lncRNA marker and application thereof
EP3548005A4 (en) 2016-11-29 2020-06-17 Puretech Health LLC Exosomes for delivery of therapeutic agents
CN108251529B (en) * 2018-02-01 2021-06-01 新乡医学院 Application of ANO5 in preparation of medicine for treating pancreatic cancer
CN110564771B (en) * 2018-06-05 2022-09-13 中国科学院脑科学与智能技术卓越创新中心 Preparation method of cerebral calcification disease model
CN108815512B (en) * 2018-08-23 2021-06-11 河南师范大学 Anticancer medicine and its application
CN112553333B (en) * 2020-12-08 2022-03-08 南方医科大学深圳医院 Application of miR-1207 and target gene thereof in detection of laryngeal squamous cell carcinoma
JP2024500035A (en) 2020-12-23 2024-01-04 アルゴノート アールエヌエー リミテッド Treatment of cardiovascular diseases
WO2023041508A2 (en) 2021-09-14 2023-03-23 Argonaute RNA Limited Treatment of cardiovascular disease

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002044321A2 (en) * 2000-12-01 2002-06-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Rna interference mediating small rna molecules
WO2002097114A2 (en) * 2001-05-29 2002-12-05 Sirna Therapeutics, Inc. Nucleic acid treatment of diseases or conditions related to levels of ras, her2 and hiv
WO2003070918A2 (en) * 2002-02-20 2003-08-28 Ribozyme Pharmaceuticals, Incorporated Rna interference by modified short interfering nucleic acid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002081628A2 (en) * 2001-04-05 2002-10-17 Ribozyme Pharmaceuticals, Incorporated Modulation of gene expression associated with inflammation proliferation and neurite outgrowth, using nucleic acid based technologies
AU3811101A (en) * 2000-02-11 2001-08-20 Lawrence Blatt Method and reagent for the modulation and diagnosis of cd20 and nogo gene expression
WO2003070887A2 (en) * 2002-02-20 2003-08-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF POLYCOMB GROUP PROTEIN EZH2 GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
AU2003207708A1 (en) * 2002-02-20 2003-09-09 Sirna Therapeutics, Inc. Rna interference mediated inhibition of map kinase genes
EP1448580A4 (en) * 2002-02-20 2005-07-20 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002044321A2 (en) * 2000-12-01 2002-06-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Rna interference mediating small rna molecules
WO2002097114A2 (en) * 2001-05-29 2002-12-05 Sirna Therapeutics, Inc. Nucleic acid treatment of diseases or conditions related to levels of ras, her2 and hiv
WO2003070918A2 (en) * 2002-02-20 2003-08-28 Ribozyme Pharmaceuticals, Incorporated Rna interference by modified short interfering nucleic acid

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE NCBI 9 June 2005 (2005-06-09), XP002341785, Database accession no. NM_002524 *
ELBASHIR S M ET AL: "Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate", EMBO JOURNAL, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 20, no. 23, 3 December 2001 (2001-12-03), pages 6877 - 6888, XP002225998, ISSN: 0261-4189 *
ESKANDARPOUR MALIHE ET AL: "Suppression of oncogenic NRAS by RNA interference induces apoptosis of human melanoma cells", INTERNATIONAL JOURNAL OF CANCER, vol. 115, no. 1, May 2005 (2005-05-01), pages 65 - 73, XP002341769, ISSN: 0020-7136 *

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