ATE522811T1 - Reagentien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptor-farbstoff - Google Patents

Reagentien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptor-farbstoff

Info

Publication number
ATE522811T1
ATE522811T1 AT08756173T AT08756173T ATE522811T1 AT E522811 T1 ATE522811 T1 AT E522811T1 AT 08756173 T AT08756173 T AT 08756173T AT 08756173 T AT08756173 T AT 08756173T AT E522811 T1 ATE522811 T1 AT E522811T1
Authority
AT
Austria
Prior art keywords
kits
reagents
energy
methods
acceptor dye
Prior art date
Application number
AT08756173T
Other languages
English (en)
Inventor
Rouh-Rong Juo
John Voyta
Brooks Edwards
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE522811T1 publication Critical patent/ATE522811T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT08756173T 2007-05-23 2008-05-23 Reagentien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptor-farbstoff ATE522811T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92464007P 2007-05-23 2007-05-23
PCT/US2008/064643 WO2008147949A1 (en) 2007-05-23 2008-05-23 Reagents, kits and methods for detecting biological molecules by energy transfer from an activated chemiluminescent substrate to an energy acceptor dye

Publications (1)

Publication Number Publication Date
ATE522811T1 true ATE522811T1 (de) 2011-09-15

Family

ID=39673350

Family Applications (2)

Application Number Title Priority Date Filing Date
AT09180519T ATE533052T1 (de) 2007-05-23 2008-05-23 Reagenzien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptorfarbstoff
AT08756173T ATE522811T1 (de) 2007-05-23 2008-05-23 Reagentien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptor-farbstoff

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT09180519T ATE533052T1 (de) 2007-05-23 2008-05-23 Reagenzien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptorfarbstoff

Country Status (6)

Country Link
US (3) US8865478B2 (de)
EP (3) EP2165197B1 (de)
JP (1) JP2010528294A (de)
CN (1) CN101784897A (de)
AT (2) ATE533052T1 (de)
WO (1) WO2008147949A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2165197B1 (de) 2007-05-23 2011-08-31 Life Technologies Corporation Reagentien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptor-farbstoff
AU2010273431B2 (en) * 2009-07-16 2016-08-25 Beckman Coulter, Inc. Novel fluorescent dyes and uses thereof
US20110097723A1 (en) * 2009-09-19 2011-04-28 Qun Liu Methods and reagents for analyte detection
US20110306148A1 (en) * 2010-06-14 2011-12-15 Siemens Healthcare Diagnostics Inc. Composition for use as an assay reagent
US9637773B2 (en) 2011-01-13 2017-05-02 Enzo Life Sciences, Inc. Compounds and methods for detection of enzymes that remove formyl, succinyl, methyl succinyl or myristoyl groups from ε-amino lysine moieties
CN102175856A (zh) * 2011-01-20 2011-09-07 郑州大学 磺胺六甲氧嘧啶检测试剂盒、其制备方法及其应用
WO2012151142A2 (en) * 2011-05-03 2012-11-08 Life Technologies Corporation Flash and glow 1,2-dioxetanes
CN102504060B (zh) * 2011-09-16 2013-10-30 淮海工学院 含有季铵基和香豆素酰腙的多功能聚苯乙烯螯合树脂及其应用
CN103868913B (zh) * 2014-02-11 2017-07-14 中生北控生物科技股份有限公司 碱性磷酸酶的酶促化学发光底物液
US20160033497A1 (en) * 2014-08-02 2016-02-04 Tianxin Wang Luminescence methods and reagents for analyte detection
CN106753341B (zh) * 2016-12-27 2019-03-19 湘潭大学 一种近红外碱性磷酸酶荧光探针的制备方法和应用
KR101986431B1 (ko) * 2017-11-21 2019-06-07 한국교통대학교산학협력단 박테리아 진단, 사멸기능을 갖는 유기공중합체, 탄소양자점 및 그 제조방법
CN109187514B (zh) * 2018-11-28 2020-12-08 广西壮族自治区农业科学院农产品质量安全与检测技术研究所 一种基于纳米金表面的化学发光共振能量转移传感器快速检测百草枯的方法
DE102019219531A1 (de) * 2019-12-13 2021-06-17 Robert Bosch Gmbh Verfahren zur Durchführung einer Amplifikationsreaktion in einer mikrofluidischen Vorrichtung
CN112903664A (zh) * 2021-01-26 2021-06-04 金华市鑫科医疗器械有限公司 一种碱性磷酸酶的酶促化学发光底物液

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340590C (en) 1986-07-24 1999-06-08 John C. Voyta Chemiluminescence enhancement
US5169788A (en) * 1989-06-09 1992-12-08 New England Deaconess Hospital Corporation Methods of measuring membrane potential using j-aggregate forming dyes
US5071909A (en) * 1989-07-26 1991-12-10 Millipore Corporation Immobilization of proteins and peptides on insoluble supports
US5547836A (en) 1990-08-30 1996-08-20 Tropix, Inc. Enhancement of chemiluminescent assays
US5336596A (en) 1991-12-23 1994-08-09 Tropix, Inc. Membrane for chemiluminescent blotting applications
US5451347A (en) 1993-06-24 1995-09-19 Lumigen, Inc. Methods and compositions providing enhanced chemiluminescence from chemiluminescent compounds using dicationic surfactants
US6028190A (en) * 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
WO1997014692A1 (en) * 1995-10-17 1997-04-24 Tropix, Inc. 1,2 chemiluminescent dioxetanes of improved performance
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US6860980B2 (en) 2000-09-15 2005-03-01 The United States Of America As Represented By The Secretary Of Commerce Polyelectrolyte derivatization of microfluidic devices
KR20020053514A (ko) 2000-12-27 2002-07-05 이구택 냉연강판의 두께편차 제어장치
US7368296B2 (en) * 2002-01-17 2008-05-06 Applied Biosystems Solid phases optimized for chemiluminescent detection
EP1542013B1 (de) * 2002-06-24 2016-04-13 Fujirebio Inc. Chemilumineszenzverbesserer
US20050026151A1 (en) * 2003-07-17 2005-02-03 Voyta John C. Simultaneous generation of multiple chemiluminescent signals on solid supports
DE03405645T1 (de) * 2003-09-04 2005-09-01 Rolex Sa Entfärbungsbeständige Uhr oder Schmuck
US7910753B2 (en) * 2004-09-10 2011-03-22 Anaspec Incorporated Cyanine dyes and their applications as luminescence quenching compounds
EP2165197B1 (de) 2007-05-23 2011-08-31 Life Technologies Corporation Reagentien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptor-farbstoff

Also Published As

Publication number Publication date
ATE533052T1 (de) 2011-11-15
EP2175272B1 (de) 2011-11-09
US8865478B2 (en) 2014-10-21
EP2175272A3 (de) 2010-07-28
WO2008147949A1 (en) 2008-12-04
EP2175272A2 (de) 2010-04-14
US20090047688A1 (en) 2009-02-19
CN101784897A (zh) 2010-07-21
US20170153229A1 (en) 2017-06-01
US20150010930A1 (en) 2015-01-08
EP2165197A1 (de) 2010-03-24
EP2165197B1 (de) 2011-08-31
EP2390663B1 (de) 2013-11-13
EP2390663A1 (de) 2011-11-30
JP2010528294A (ja) 2010-08-19

Similar Documents

Publication Publication Date Title
ATE533052T1 (de) Reagenzien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptorfarbstoff
DE602004028445D1 (de) Lichtdurchlässige festmatrix-prüfvorrichtung zur mikroarray-analyse
JP2016512429A5 (de)
ATE524736T1 (de) Sequenzielle analyse biologischer proben mit bleichung von intermediären fluoreszenzsignalen
WO2005088308A3 (en) Fluorescent signal emitting live cell biosensor molecules and dyes for detection and quantification of protein activities
TW200801203A (en) Multiplexed analyses of test samples
ATE489482T1 (de) Verfahren zur herstellung von hybridsubstraten mit dna und antikörpern sowie verwendungen davon
ATE477338T1 (de) Vorrichtung und verfahren zum nachweis von molekularen wechselwirkungen
WO2009058902A3 (en) Methods and devices for analyte detection
WO2011056185A3 (en) Reactivity-dependent and interaction-dependent pcr
ATE526333T1 (de) Reagentien zum nachweis von hypochloriger säure
MX2013003161A (es) Dispositivos, metodos y kits de inmunocromatografia.
ATE511636T1 (de) Verfahren zum nachweis biologischer organismen mittels raman-streuung
ATE462803T1 (de) Kit und verfahren zum nachweis von urothelkarzinom
ATE447712T1 (de) Immunoassay mit doppelter standardkurve
UA119466C2 (uk) Спосіб виявлення активних форм аналіту та визначення здатності речовин зв'язуватися з активними центрами аналіту
WO2008117792A1 (ja) アデノシン三リン酸測定のための蛍光標識融合タンパク質
ATE523603T1 (de) Konjugatkomplexe zum analytnachweis
WO2008123180A1 (ja) 標的物質の検出方法、並びに、これに用いるタグ、ｄｎａ、ベクター、プローブ及び検出キット
ATE533053T1 (de) Nicht-elispot-test
ATE394417T1 (de) Sonde zum nachweis von nukleinsäuren
WO2009014613A3 (en) An automated synthesis or sequencing apparatus and method for making and using same
EE01561U1 (et) Meetod vedelas proovis vähemalt ühe orgaanilise analüüdi tuvastamiseks ja kvantifitseerimiseks.
ATE477488T1 (de) Verfahren zur bestimmung einer dna- bindungsprotein-menge
JP2007000082A5 (de)

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties