ATE516349T1 - Verfahren zur rückfaltung von säuger- glycosyltransferasen - Google Patents

Verfahren zur rückfaltung von säuger- glycosyltransferasen

Info

Publication number
ATE516349T1
ATE516349T1 AT05713041T AT05713041T ATE516349T1 AT E516349 T1 ATE516349 T1 AT E516349T1 AT 05713041 T AT05713041 T AT 05713041T AT 05713041 T AT05713041 T AT 05713041T AT E516349 T1 ATE516349 T1 AT E516349T1
Authority
AT
Austria
Prior art keywords
refolding
mammal
refolded
glycosyltransferases
glycosyl transferases
Prior art date
Application number
AT05713041T
Other languages
English (en)
Inventor
Sami Saribas
David Hakes
Scott Willett
Karl Johnson
Daniel James Bezila
Shawn Defrees
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE516349T1 publication Critical patent/ATE516349T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT05713041T 2004-02-04 2005-02-04 Verfahren zur rückfaltung von säuger- glycosyltransferasen ATE516349T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US54221004P 2004-02-04 2004-02-04
US59940604P 2004-08-06 2004-08-06
US62740604P 2004-11-12 2004-11-12
PCT/US2005/003856 WO2005089102A2 (en) 2004-02-04 2005-02-04 Methods of refolding mammalian glycosyltransferases

Publications (1)

Publication Number Publication Date
ATE516349T1 true ATE516349T1 (de) 2011-07-15

Family

ID=34994147

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05713041T ATE516349T1 (de) 2004-02-04 2005-02-04 Verfahren zur rückfaltung von säuger- glycosyltransferasen

Country Status (10)

Country Link
EP (2) EP2484759B1 (de)
JP (1) JP4892358B2 (de)
AT (1) ATE516349T1 (de)
AU (1) AU2005222782B2 (de)
CA (1) CA2555109C (de)
ES (1) ES2501642T3 (de)
IL (1) IL177179A (de)
MX (1) MXPA06008812A (de)
NZ (1) NZ548893A (de)
WO (1) WO2005089102A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080021590A (ko) 2005-03-24 2008-03-07 네오스 테크놀로지스, 인크. 가용성이고 활성인 진핵생물 글리코실트랜스퍼라제의원핵생물 유기체 내에서의 발현
US20080249286A1 (en) * 2007-04-05 2008-10-09 Seefeldt Matthew B High-pressure refolding of proteins in the presence of binding partners
WO2008143944A2 (en) * 2007-05-14 2008-11-27 Government Of The Usa, As Represented By The Secretary, Department Of Health And Human Services Methods of glycosylation and bioconjugation
JP5358840B2 (ja) * 2007-11-24 2013-12-04 独立行政法人産業技術総合研究所 Ｇｆｐ（緑色蛍光蛋白質）の機能賦活・回復方法
EA020621B1 (ru) 2009-06-22 2014-12-30 Амген Инк. Рефолдинг белков с использованием химически контролируемого окислительно-восстановительного состояния
JP2012531428A (ja) 2009-06-25 2012-12-10 アムジエン・インコーポレーテツド 哺乳類以外の系で発現されるタンパク質の捕獲精製プロセス
ES2930534T3 (es) 2017-09-04 2022-12-16 89Bio Ltd Conjugados de péptidos de FGF-21 mutantes y usos de los mismos
AU2019349934B2 (en) * 2018-09-25 2024-12-05 Absci Corporation Protein purification methods

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US232263A (en) 1880-09-14 Heating stove and grate
US4179337A (en) 1973-07-20 1979-12-18 Davis Frank F Non-immunogenic polypeptides
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4935349A (en) 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
US5272066A (en) 1986-03-07 1993-12-21 Massachusetts Institute Of Technology Synthetic method for enhancing glycoprotein stability
GB8612087D0 (en) 1986-05-19 1986-06-25 Ici Plc Hybridisation probes
US5017478A (en) 1987-07-16 1991-05-21 Berlex Laboratories, Inc. Transfected cells containing plasmids having genes oriented in opposing directions and methods of using
US5106739A (en) 1989-04-18 1992-04-21 Calgene, Inc. CaMv 355 enhanced mannopine synthase promoter and method for using same
US5032519A (en) 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
JP3476455B2 (ja) 1991-03-18 2003-12-10 ザ スクリップス リサーチ インスティテュート ＮｅｕＡｃα２、６Ｇａｌβ１、４ＧｌｃＮＡｃ及びシアリルＬｅｘの合成法
US5352670A (en) 1991-06-10 1994-10-04 Alberta Research Council Methods for the enzymatic synthesis of alpha-sialylated oligosaccharide glycosides
US5374655A (en) 1991-06-10 1994-12-20 Alberta Research Council Methods for the synthesis of monofucosylated oligosaccharides terminating in di-N-acetyllactosaminyl structures
US5414085A (en) 1992-04-06 1995-05-09 Biosite Diagnostics, Inc. Barbiturate derivatives and protein and polypeptide barbiturate derivative conjugates and labels
WO1994012646A1 (en) 1992-11-27 1994-06-09 Ciba-Geigy Ag Proteins having glycosyltransferase activity
US5374541A (en) 1993-05-04 1994-12-20 The Scripps Research Institute Combined use of β-galactosidase and sialyltransferase coupled with in situ regeneration of CMP-sialic acid for one pot synthesis of oligosaccharides
US5409817A (en) 1993-05-04 1995-04-25 Cytel, Inc. Use of trans-sialidase and sialyltransferase for synthesis of sialylα2→3βgalactosides
US5426039A (en) 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
US5691180A (en) 1994-06-09 1997-11-25 The Regents Of The University Of Michigan DNA sequence encoding N-acetyl-galactosamine-transferase
US5545553A (en) 1994-09-26 1996-08-13 The Rockefeller University Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them
US5728554A (en) 1995-04-11 1998-03-17 Cytel Corporation Enzymatic synthesis of glycosidic linkages
US5716812A (en) 1995-12-12 1998-02-10 The University Of British Columbia Methods and compositions for synthesis of oligosaccharides, and the products formed thereby
AU735695B2 (en) 1996-10-10 2001-07-12 Neose Technologies, Inc. Carbohydrate purification using ultrafiltration, reverse osmosis and nanofiltration
US6117651A (en) 1996-11-08 2000-09-12 Neose Technologies, Inc. Expression vectors
WO1999015636A1 (en) 1997-09-24 1999-04-01 Novo Nordisk A/S Expression of starch-binding domain (sbd)
US7244601B2 (en) * 1997-12-15 2007-07-17 National Research Council Of Canada Fusion proteins for use in enzymatic synthesis of oligosaccharides
US6037730A (en) * 1998-11-12 2000-03-14 Eaton Corporation Degraded electronic throttle operation method and system

Also Published As

Publication number Publication date
IL177179A (en) 2012-04-30
EP1718739A2 (de) 2006-11-08
EP2484759B1 (de) 2014-06-25
WO2005089102A3 (en) 2007-04-26
ES2501642T3 (es) 2014-10-02
EP2484759A3 (de) 2012-11-21
CA2555109C (en) 2016-12-06
JP4892358B2 (ja) 2012-03-07
EP1718739A4 (de) 2009-08-05
AU2005222782B2 (en) 2010-06-17
EP1718739B1 (de) 2011-07-13
IL177179A0 (en) 2008-03-20
AU2005222782A1 (en) 2005-09-29
MXPA06008812A (es) 2008-03-07
NZ548893A (en) 2009-09-25
EP2484759A2 (de) 2012-08-08
JP2007535309A (ja) 2007-12-06
WO2005089102A2 (en) 2005-09-29
WO2005089102A9 (en) 2006-03-16
CA2555109A1 (en) 2005-09-29

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